sp|Q8LBL5|PRT1_ARATH ----> E3 ubiquitin-protein ligase PRT1

Intron 1 GCACTCTAAG ... CTCACGAGAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	29.83	127	54	0
A2	34.26	165	86	2
A3	33.33	170	85	0
A4	32.41	146	70	0
A5	31.48	74	34	2
A6	30.46	105	46	0
A7	29.54	353	148	0
A8	29.39	161	67	0
A9	34.18	260	135	0
A10	27.17	386	144	2

>TRINITY_DN38074_c0_g1_i2 from sample A10 / Example spliced isoform

AGAAAACCTACAGTTAAAATAGCGCGAATCCCCCTATTTTGGAGGGGAAACTGACTTCTATGTATTTGGGTGGCTAACTTCAACGTCGTGCGCATTTGATGGATTCACGGAATGAGAATCTCGATATCAGCAGAATGGATGGAGTTGATGAGCCAAGGGAGAAGGAAGTCGCGGCGTCGGAGAATTTGGCGGAAAGGAAT
GGCGACATGGAGAGAAAGATGGAAGGTTCAAACTCTCGTCCTTCAATAGCCATGGATGTGGATGATGGTAGCGAGTTCTTCCTTTGTGTAATATGCCTGGAGCTGGTGTACAAGCCTATCGTTCAGTCTTGCGGTCATCTCTTCTGCTTCTGGTGTGTTCATCGAGCCATGAACAGTGACACAGAATCTTACTGTCCTCT
CTGTAGGAGTCCATACAATCATTTTCCAAGAGTGTGTGAATTGCTTCACTTTCTTCTCTTTAAAGCATTTCCAGAGGATTACAGACAAAGAGCAGAGGAAGTACTAGAACAAGAGAGAACATTAGAAATCTTTTCTCCCCAATTCGGTGATCCTCAAATTGCCAAAATCATGGATGATTCAAAGTTGAGTAGCTCAGAAA
CTACTGCAAATTTTCCTGAAAGGGAATCCTCACAAGAAGATTCACTTTCAGAATCAAATGTTGCAACAAAATGCATGGACAGTATTTTATCATCTGGCCTTATTAGGAATGGAACTGATTCAAGGCATGCATCTTCTCTACGTCATCAATGTGTATCGGTCAATGTTGAAACATCAGTGAGCATCAACAGTCTTGAAGCA
AACGATGGTTCAGAAAGTGTAGTTTCCAGAACAACAAATGGTCAGGCAAATGCACCAAGTCTTATTTCATTTAATAAAGTTACAGTGGAGGATGCTTTGTGTTGCTACTGCAAAAGGTTACTGTATCATCCTACAGTCCTCAATTGTGGCCATGTCTTTTGTCAATCATGCATCAAGACCTCTGAAGAGAGAACTCTTAA
CTGCCAAGTTTGTCAAAGTTTACACCCTGGAGATCAACCATGTGTTTGTTTGCGGTTGCACCAGTTCTTGGAGCAGACATTTACTGTAGAATATGCTGAAAGGAAACAATCAGTTCTTGTCCAAGAAAAAATGCACCAACCAAGATGTATTTCCTACAAATCTCTTCCAGTCCTGTTAGGTCTTCATCATTATGCGAGCC
AAGGCTATTGTTCTTTCTAAGGACCAAAAGCCTTTTTTCCTGTGGGCTAGATCATAGAAGTGTATTAAGGAAATTATGCTGAAACTGCTCATTGC

>TRINITY_DN38074_c0_g1_i4 from sample A10 / Example unspliced isoform:

AGAAAACCTACAGTTAAAATAGCGCGAATCCCCCTATTTTGGAGGGGAAACTGACTTCTATGTATTTGGGTGGCTAACTTCAACGTCGTGCGCATTTGATGGATTCACGGAATGAGAATCTCGATATCAGCAGAATGGATGGAGTTGATGAGCCAAGGGAGAAGGAAGTCGCGGCGTCGGAGAATTTGGCGGAAAGGAAT
GGCGACATGGAGAGAAAGATGGAAGGTTCAAACTCTCGTCCTTCAATAGCCATGGATGTGGATGATGGTAGCGAGTTCTTCCTTTGTGTAATATGCCTGgcactctaagagaaactgattttcatcctcaacactcatagtctcatagagatgacaaattctttcaacccacagtgttttaggaatcgtccgatgcccta
tttcaatatggggctcacgagagGAGCTGGTGTACAAGCCTATCG

Intron 2 GTAAGCCAGT ... TTTTTGGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	26.51	60.7	8.7	15
A2	18.62	72.1	7.9	12
A3	28.94	68	5.6	22
A4	23.12	69.1	8.9	16
A5	0	29.6	3.1	0
A6	25.33	57.1	3.9	17
A7	18.59	167.6	13.3	27
A8	18.15	74.9	6.7	12
A9	15.57	105.9	10.1	15
A10	19.49	193.4	13.4	34

>TRINITY_DN130166_c2_g1_i5 from sample A1 / Example spliced isoform

GCGTGGGCTAAATTAATTGCAGAGAAAACCTACAGTTAAAATAGCGCGAATCCCCCTATTTTGGAGGGGAAACTGACTTCTATGTATTTGGGTGGCTAACTTCAACGTCGTGCGCATTTGATGGATTCACGGAATGAGAATCTCGATATCAGCAGAATGGATGGAGTTGATGAGCCAAGGGAGAAGGAAGTCGCGGCGTC
GGAGAATTTGGCGGAAAGGAATGGCGACATGGAGAGAAAGATGGAAGGTTCAAACTCTCGTCCTTCAATAGCCATGGATGTGGATGATGGTAGCGAGTTCTTCCTTTGTGTAATATGCCTGGAGCTGGTGTACAAGCCTATCGTTCAGTCTTGCGGTCATCTCTTCTGCTTCTGGTGTGTTCATCGAGCCATGAACAGTG
ACACAGAATCTTACTGTCCTCTCTGTAGGAGTCCATACAATCATTTTCCAAGAGTGTGTGAATTGCTTCACTTTCTTCTCTTTAAAGCATTTCCAGAGGATTACAGACAAAGAGCAGAGGAAGTACTAGAACAAGAGAGAACATTAGAAATCTTTTCTCCCCAATTCGGTGATCCTCAAATTGCCAAAATCATGGATGAT
TCAAAGTTGAGTAGCTCAGAAACTACTGCAAATTTTCCTGAAAGGGAATCCTCACAAGAAGATTCACTTTCAGAATCAAATGTTGCAACAAAATGCATGGACAGTATTTTATCATCTGGCCTTATTAGGAATGGAACTGATTCAAGGCATGCATCTTCTCTACGTCATCAATGTGTATCGGTCAATGTTGAAACATCAGT
GAGCATCAACAGTCTTGAAGCAAACGATGGTTCAGAAAGTGTAGTTTCCAGAACAACAAATGGTCAGGCAAATGCACCAAGTCTTATTTCATTTAATAAAGTTACAGTGGAGGATGCTTTGTGTTGCTACTGCAAAAGGTTACTGTATCATCCTACAGTCCTCAATTGTGGCCATGTCTTTTGTCAATCATGCATCAAGA
CCTCTGAAGAGAGAACTCTTAACTGCCAAGTTTGTCAAAGTTTACACCCTGGAGATCAACCATGTGTTTGTTTGCGGTTGCACCAGTTCTTGGAGCAGACATTTACTGTAGAATATGCTGAAAGGAAACAATCAGTTCTTGTCCAAGAAAAAATGCACCAACCAAGATGCACAAAAGAAGCCAAAAGAACAATCGCTGAG
ATGCTCGAGATCCACCCAGGTGCTGGATGTGATTATTGTGGGGTGATGCCAATCGTAGGAAAGAGGTATAAATGTCTAGATTGTGAAGAGTCTATAGGGTTTGACTTGTGTGGAACATGTTATGAGAAAGGTTCAATGCTCCCTGGGCGGTTTAATCAACAGCATACATCAGAACATCATTTCCAGGAAATCGCGATCAG
ATATGAGGAATATCTGACACATCTCTTTCCGGTACTCATTAATCAGTTATCTAGGACAAGCTCGATAGTACAGATGGGTGAAGAAGACAGTGAAAGCTCAATGATTCATAACAGTGCAGAGAGCTCTGAGAATTCTGAGAGATCAAACTGAAAGTGATGAAGATTGATGTCATCACTGTGTATAGGGGTTTAATGAAGTG
TCAAATTTGTTGTAGTATGATGTTTCCATGACACCATGCATAGGGATTTGTGTTAACGATATCTGTCATATTTGCTATTGTATTGCTATACGCAACTTCTCAAAATTGCAAAGTGCATAGGAATTTAAGTTACTAGACTGGCTAAAAATCTTTAGGTCATCAGCTGTATGTTTAACTTTATACAACTTTTACTATCTAAT
GAAAAGCAGTTGAGTGATGC

>TRINITY_DN130166_c2_g1_i3 from sample A1 / Example unspliced isoform:

GGTGTACAAGCCTATCGTTCAGTgtaagccagtgcttgctcctgtctcttgttggattatactcttaaatgaacagatggtttatgttcaaagaaattcgtttaagaaacttaagttcattcgagttgaaagtacctttttttggcagCTTGCGGTCATCTCTTCTGCTTCTGGTGTGTTCATCGAGCCATGAACAGTGA
CACAGAATCTTACTGTCCTCTCTGTAGGAGTCCATACAATCATTTTCCAAGAGTGTGTGAATTGCTTCACTTTCTTCTCTTTAAAGCATTTCCAGAGGATTACAGACAAAGAGCAGAGGAAGTACTAGAACAAGAGAGAACATTAGAAATCTTTTCTCCCCAATTCGGTGATCCTCAAATTGCCAAAATCATGGATGATT
CAAAGTTGAGTAGCTCAGAAACTACTGCAAATTTTCCTGAAAGGGAATCCTCACAAGAAGATTCACTTTCAGAATCAAATGTTGCAACAAAATGCATGGACAGTATTTTATCATCTGGCCTTATTAGGAATGGAACTGATTCAAGGCATGCATCTTCTCTACGTCATCAATGTGTATCGGTCAATGTTGAAACATCAGTG
AGCATCAACAGTCTTGAAGCAAACGATGGTTCAGAAAGTGTAGTTTCCAGAACAACAAATGGTCAGGCAAATGCACCAAGTCTTATTTCATTTAATAAAGTTACAGTGGAGGATGCTTTGTGTTGCTACTGCAAAAGGTTACTGTATCATCCTACAGTCCTCAATTGTGGCCATGTCTTTTGTCAATCATGCATCAAGAC
CTCTGAAGAGAGAACTCTTAACTGCCAAGTTTGTCAAAGTTTACACCCTGGAGATCAACCATGTGTTTGTTTGCGGTTGCACCAGTTCTTGGAGCAGACATTTACTGTAGAATATGCTGAAAGGAAACAATCAGTTCTTGTCCAAGAAAAAATGCACCAACCAAGATGCACAAAAGAAGCCAAAAGAACAATCGCTGAGA
TGCTCGAGATCCACCCAGGTGCTGGATGTGATTATTGTGGGGTGAGCAATATTTGATCTCATCTTTTGGAGCCTTTTGGTGTGATCTGGTTTTGAGAAGTACTTTTCCAAAGAG