NA ----> NA

Intron 1 GTGTGTTTGT ... GTTGAAACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	16.67	5	0	1
A2	25	3	0	1
A3	33.33	2	1	1
A4	50	2	0	2
A5	30.55	7	0	3
A6	13.95	74	0	12
A7	30.05	39.7	0	17
A8	32.63	47.5	1	23
A9	31.08	35.5	1	16
A10	26.18	36.7	2	13

>TRINITY_DN43529_c0_g5_i2 from sample A8 / Example spliced isoform

TTTATTATCTTTCTGTTCATGATCCCACATGTGGAACTCTGAAGTATTTGGAACGTAGGAAATCAGCCAGGAAAAGCATTTCTGGTTCTAGATTATTAAGCAAAAGGGCTGCAAATTTATCAGGTCCAGGTGCAATTCAGGCCAAGCCACCTGCGTGCTATGCTGAGGATGTACCGTACCCACACAAGTCCTATGAGATT
AGTGTGCAACAAATTCGAAGATCTGCAGTAGCTCAAGCACAAGCAGTTGGCGCCCCTGGCAATTTTAAAAGCTTTGATTCTCAATTTGGAAACTATCTTGTACCTGTCATCCCCACTCATCTTGTGAACAGGTCACAACTTTTACCTACAGAAGGTGAAGCCATGGTGCCATCATCTTTGACACCAAAATAGTTTTTTAA
CCATTCTTTCAGCATCCTTTCATCAGCATTCATATTCATTGTTAGCTGCTTCTTTTGGAACAGTTTTCAGAGTTTAGTTTATTTATTGATCATTTCAATTTCTTTTATTTGTGATGAGTCAAATTTACATCATTATTGCATCAAACTTTGTGAGAAATTGACCTAAAGCATGGATGCACTATTTTCATGGCTTTCCTCTT
TTACATAGGCAGTGATTTGACAAGGGTAGGGTTATGTCAAGGAGCATGCAACTTCTGCAAGTTGAATTCACAATTTGCATCCTTACTAAACTGCTGCCATGGATATAGCTAGCTAGAATGTGTTTTATATGCCCATCGTAACAATCAGTATCTTAGTTTGGGATGGATATTTTTGAATGATATTGTAAAGGCACTTTTTA
TGCACAAACATATAGGCCTCCCCGCATCGCCAATTTCTTCATAAAAAATATATGCTCAACTTATGGGTTGAATTAGTGAAATCATGCATCTACCATCAATCCCTCCAATCGATGGAGTGATTTGATGCTCTGGATGAGCAATATGCTTGAAATTCTGTCTAAAATTGTTG

>TRINITY_DN43529_c0_g5_i3 from sample A8 / Example unspliced isoform:

CAATTTTGTTCTTTCAACATGTGTCTTATTATTATGTAGTCCTCTTTCGTTTCTACAGATTTATGTTAAATTGCTAATTGTTTTTGTATTTGCTCAGGGCTGCAAATTTATCAGGTCCAGGTGCAATTCAGGCCAAGCCACCTGCGTGCTATGCTGAGgtgtgtttgttcattttcgttttctccttctaggtaaaagta
tgatctgttaagctggtatcaattattggaattgaagtcatgttgaaacagGATGTACCGTACCCACACAAGTCCTATGAGATTAGTGTGCAACAAATTCGAAGATCTGCAGTAGCTCAAGCACAAGCAGTTGGCGCCCCTGGCAATTTTAAAAGCTTTGATTCTCAATTTGGAAACTATCTTGTACCTGTCATCCCCAC
TCATCTTGTGAACAGGTCACAACTTTTACCTACAGAAGGTGAAGCCATGGTGCCATCATCTTTGACACCAAAATAGTTTTTTAACCATTCTTTCAGCATCCTTTCATCAGCATTCATATTCATTGTTAGCTGCTTCTTTTGGAACAGTTTTCAGAGTTTAGTTTATTTATTGATCATTTCAATTTCTTTTATTTGTGATG
AGTCAAATTTACATCATTATTGCATCAAACTTTGTGAGAAATTGACCTAAAGCATGGATGCACTATTTTCATGGCTTTCCTCTTTTACATAGGCAGTGATTTGACAAGGGTAGGGTTATGTCAAGGAGCATGCAACTTCTGCAAGTTGAATTCACAATTTGCATCCTTACTAAACTGCTGCCATGGATATAGCTAGCTAG
AATGTGTTTTATATGCCCATCGTAACAATCAGTATCTTAGTTTGGGATGGATATTTTTGAATGATATTGTAAAGGCACTTTTTATGCACAAACATATAGGCCTCCCCGCATCGCCAATTTCTTCATAAAAAATATATGCTCAACTTATGGGTTGAATTAGTGAAATCATGCATCTACCATCAATCCCTCCAATCGATGGA
GTGATTTGATGCTCTGGATGAGCAATATGCTTGAAATTCTGTCTAAAATTGTTG

Intron 2 GTGAAGCCAT ... ACATAGGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	47.83	6	6	5
A2	60	1	1	2
A3	81.82	1	6	3
A4	100	0	3	1
A5	50	2	2	2
A6	75.48	19	84	33
A7	74.12	11	30	33
A8	70.91	24	74	43
A9	70.27	22	61	43
A10	72.73	12	44	20

>TRINITY_DN35652_c0_g1_i2 from sample A9 / Example spliced isoform

TGGGGAAGTATGGATGAGTTTCCTCCCAGAAAAGAAAAGCGTTTACGTATAAGTATAATCCCCGCTGGATTAATAAAACTTTTCCAATCATATGAAAGAGAATAAAGCTTCTACGAAATTTATGTTGATGGCAAAGATCATAGGGGCCACCCTGAACCATTTGAAAGAAAATCAAAGAAATTTATGTTGATGGATTGATA
CGCAGAGTATCTTTTATGAATCCATGGACAAGATCACAGAGCCCGTTTGAACCATCTTAAGAAGATTCAAATTCACACTACTGAAGGTTTCAACTTCTGTCCCGGCTCCTCAGACGCAGTCGTCAATTCTTTTTGACCACGACATCGGAGGACTGTAATTGTATTAGTGTTTGAGGTCCAGCAATTCTATGGCAGAGGCT
GCAAAAATGCGCGACCAGCCCAAGCCACGACGTACCACGCTAAACAAGTCTCCATGGAAATCAGCCAGGAAAAGCATTTCTGGTTCTAGATTATTAAGCAAAAGGGCTGCAAATTTATCAGGTCCAGGTGCAATTCAGGCCAAGCCACCTGCGTGCTATGCTGAGGATGTACCGTACCCACACAAGTCCTATGAGATTAG
TGTGCAACAAATTCGAAGATCTGCAGTAGCTCAAGCACAAGCAGTTGGCGCCCCTGGCAATTTTAAAAGCTTTGATTCTCAATTTGGAAACTATCTTGTACCTGTCATCCCCACTCATCTTGTGAACAGGTCACAACTTTTACCTACAGAAGTGATTTGACAAGGGTAGGGTTATGTCAAGGAGCATGCAACTTCTGCAA
ATTGAATTCACAATTTGCATCCTTACTAAACTGCTGCCATGGATATAGCTAGCTAGAATGTGTTTTATATGCCCATCGTAACAATCAGTATCTTAGTTTGGGATGGATATTTTTGAATGATATTGTAAAGGCACTTTTTATGCACAAACATATAGGCCTCCCCGCATCGCCAATTTCTTCATAAAAAATATATGC

>TRINITY_DN35652_c0_g1_i3 from sample A9 / Example unspliced isoform:

GCCACCTGCGTGCTATGCTGAGGTGTGTTTGTTCATTTTCGTTTTCTCCTTCTAGGTAAAAGTATGATCTGTTAAGCTGGTATCAATTATTGGAATTGAAGTCATGTTGAAACAGGATGTACCGTACCCACACAAGTCCTATGAGATTAGTGTGCAACAAATTCGAAGATCTGCAGTAGCTCAAGCACAAGCAGTTGGCG
CCCCTGGCAATTTTAAAAGCTTTGATTCTCAATTTGGAAACTATCTTGTACCTGTCATCCCCACTCATCTTGTGAACAGGTCACAACTTTTACCTACAGAAGgtgaagccatggtgccatcatctttgacaccaaaatagttttttaaccattctttcagcatcctttcatcagcattcatattcattgttagctgcttc
ttttggaacagttttcagagtttagtttatttattgatcatttcaatttcttttatttgtgatgagtcaaatttacatcattattgcgtcaaactttgtgagaaattgacctaaagcatggatgcactattttcatggctttcctcttttacataggcagTGATTTGACAAGGGTAGGGTTATGTCAAGGAGCATGCAAC
TTCTGCAAATTGAATTCACAATTTGCATCCTTACTAAACTGCTGCCATGGATATAGCTAGCTAGAATGTGTTTTATATGCCCATCGTAACAATCAGTATCTTAGTTTGGGATGGATATTTTTGAATGATATTGTAAAGGCACTTTTTATGCACAAACATATAGGCCTCCCCGCATCGCCAATTTCTTCATAAAAAATATA
TGC