NA ----> NA

Intron 1 GTATTTTCTC ... CTTTTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	11.72	610	65	97
A2	9.95	493	54	55
A3	10.12	515	40	76
A4	10.57	402	39	56
A5	13.13	172	17	35
A6	11.06	905	75	150
A7	10.42	1565	149	215
A8	12.84	1792	188	340
A9	12.6	1366	154	240
A10	11.1	1109	134	143

>TRINITY_DN41575_c0_g3_i2 from sample A8 / Example spliced isoform

ATTTAATTAGAAATGAAGAACACTATGGAGTTTCAAATAGTAATTTAGAAGGAACCATGAGGGATGATAAGGTTGAGAATGGAATCTCTGATTTGGGTTCTAGTTTGCACCAGGAGAACAAAATGAAGCTCGTGTCTTGGCCTGTGAATTTAGAACATTTGCCATCTCCAAGACGGAACTCTGGTTTGTGTTCTACTTTG
CAAGAGAATGAAATGGAGCCTGTGTCTTGGCATGTAAGATCAGAACATTTGCCATCCCTAGGCCAGAAGGAAATAGAGAAGGTATCTATTGGGTTAGACATTGTTTCAACCACTGGTTCCGTTTCAGTTCAGATTGAAAAGCAGGATGGTGCTTCCTGTCAAGTTTCTCAGGCAAGTATCTCTATGCAAGATGAGGATTT
ACATGATAATGTTAGAAATGGAAATTCTAATTTGGTTTGTCAGTTGCAGTTGGAAAATGAAATGGAGACTACATCTCAGCCTGTAAAATTTGAGCAGTCCCTGGCTACAAGAAAAGGAGAAACACAGAAGGTAACTGAAGAATTCCTCTTTTCTGAAGCCTCTAGTCTTGATAAGGGTTTGGATGTAGTTCAAAATGAAG
TAAATGATAGAGCTTGCACCAAAGCTTGCCAAGGAAGTATGAATATCCAGAGTGACTATTCAAATGACAAGGTCAAATCCATATTATCTGATGTTACTGATAGCTTGGAATTCAAAACTGGAGTGGTGTTTAATCTTAAAGAGAATGTCACAAATTCTTCCAACAATTATCAAAATAATACCACCGATACGCTTATTACT
CCATCCCTATTGTCAGGCTGTCCTCCATGTGAAGCTTTTCCTTCAGTCATAGAACATAAATCTATAGATAAAGATGATTCTTCATTACGTAATATGAAATTGTCTGATAGCTCGGTTACGCTAAAAGTGCAAATGATGTCTGGGCTCAAAGTTGCTGGTAATGAAGTTAAATTTAAGGGAAGAAGTCCTTTGGCTACCAA
TTACGAGCTGCTGAACTCTACTGCTAAGGAAATATCCCAGAAACATGTCAAAGAACAGAGGTGTTCAATGGATGGTTCAGAGAGATTGACATCCTTGCCTAGGTCATGTCCTGTATTGGATGATACAGCAGAGAAAGAGAACAGCAGGCCAGAAGGGCAAGATGGAGTTGGCATGTCAGAAGTGCATGGTGCAGATGGTC
ATTATGTAAGCTCTGGAAGAAAGTCAATGGGTAACCTAAGTGTTCAAGTACAGAAAATAAAGAACCCTTTAGATGCTTCGCCATTTTCAGAAGAGTGGATTGCTGCTATACAAGCTGTGGGTGAGGAATGGCTTCAAATGAAAAGAGGACCAGTGCAGAACTCACCACCAAACAAAATGGTTCCCCAACCAGGTCCATGG

TCTCCGGTTCGGCGCCCTAACCAGCAACTAGGTCCATTTGATTGTACTAAATATACAAATGCAGCGGACAGTACTGATGTTTATGAAGCTGAATAAGATGCCTGTGGTAGGCATGCTTTTCATGTATGGCAGAAGGGTGATCAGTCTTGTACATAGTTCCTAATTACCAATATGTACGAACTATGAAGTTACCATCTGCG
GCAAACAAAGGCATGGAATCTGGCTTGCAGGGAACTCTTATTATATGCTATGATGCCCTTATGCCAGGGTCTGTTCCAGTAGAATCTCCCATGTTTCTTGTTTTGAAAGATTGTGGTCAAGAACGATACAATAGCAGGCTAAAAGTCTGTTGGTGGAGCAGGATCTGATAGTTTGTTGCAAGTGACCTTAGTGTTGATCA
TTTTAGATATTTTGTGATTTATTCTGTTTACAAACTTGTCTTGTAAGTGAACCCATCTCAGGGCTATTGTAATCTGTTTTCATTAGGCTTTCCTTTGTTGGCTGAGAAAATTTCTCAGAAGGATCAATTTTTTGAGCATTTCAGAAATATTTCTGCATTCCAGGCTCCTAAGACAATCCAAATTACACAATGCATTGAAC
GTTGCAGTTGTGTATGGGTACTCTTATTTACTTCTGTACATGAGTGCTTGATGGTGCAGAAGAAATGATGGAATTCTTTTCATAAGTAGCGGTTTGGTGCACCAGCTACAGGAAATATATCTGGATGTTAAGAAGGTACTTGCAGCAATTGTTCATGATGTCTTTGTAATTTGGATGTATAAAACTGTAGATAATAAGCT
TCCTTTAACACATCTAATTTAAAAAAGCTGTTAAGTGTAGG

>TRINITY_DN41575_c0_g3_i4 from sample A8 / Example unspliced isoform:

ATTTAATTAGAAATGAAGAACACTATGGAGTTTCAAATAGTAATTTAGAAGGAACCATGAGGGATGATAAGGTTGAGAATGGAATCTCTGATTTGGGTTCTAGTTTGCACCAGGAGAACAAAATGAAGCTCGTGTCTTGGCCTGTGAATTTAGAACATTTGCCATCTCCAAGACGGAACTCTGGTTTGTGTTCTACTTTG
CAAGAGAATGAAATGGAGCCTGTGTCTTGGCATGTAAGATCAGAACATTTGCCATCCCTAGGCCAGAAGGAAATAGAGAAGGTATCTATTGGGTTAGACATTGTTTCAACCACTGGTTCCGTTTCAGTTCAGATTGAAAAGCAGGATGGTGCTTCCTGTCAAGTTTCTCAGGCAAGTATCTCTATGCAAGATGAGGATTT
ACATGATAATGTTAGAAATGGAAATTCTAATTTGGTTTGTCAGTTGCAGTTGGAAAATGAAATGGAGACTACATCTCAGCCTGTAAAATTTGAGCAGTCCCTGGCTACAAGAAAAGGAGAAACACAGAAGGTAACTGAAGAATTCCTCTTTTCTGAAGCCTCTAGTCTTGATAAGGGTTTGGATGTAGTTCAAAATGAAG
TAAATGATAGAGCTTGCACCAAAGCTTGCCAAGGAAGTATGAATATCCAGAGTGACTATTCAAATGACAAGGTCAAATCCATATTATCTGATGTTACTGATAGCTTGGAATTCAAAACTGGAGTGGTGTTTAATCTTAAAGAGAATGTCACAAATTCTTCCAACAATTATCAAAATAATACCACCGATACGCTTATTACT
CCATCCCTATTGTCAGGCTGTCCTCCATGTGAAGCTTTTCCTTCAGTCATAGAACATAAATCTATAGATAAAGATGATTCTTCATTACGTAATATGAAATTGTCTGATAGCTCGGTTACGCTAAAAGTGCAAATGATGTCTGGGCTCAAAGTTGCTGGTAATGAAGTTAAATTTAAGGGAAGAAGTCCTTTGGCTACCAA
TTACGAGCTGCTGAACTCTACTGCTAAGGAAATATCCCAGAAACATGTCAAAGAACAGAGGTGTTCAATGGATGGTTCAGAGAGATTGACATCCTTGCCTAGGTCATGTCCTGTATTGGATGATACAGCAGAGAAAGAGAACAGCAGGCCAGAAGGGCAAGATGGAGTTGGCATGTCAGAAGTGCATGGTGCAGATGGTC
ATTATGTAAGCTCTGGAAGAAAGTCAATGGGTAACCTAAGTGTTCAAGTACAGAAAATAAAGAACCCTTTAGATGCTTCGCCATTTTCAGAAGAGTGGATTGCTGCTATACAAGCTGTGGGTGAGAAACCCGTTTTCAGGAATGGCTTCAAATGAAAAGAGGACCAGTGCAGAACTCACCACCAAACAAAATGGTTCCCC
AACCAGGTCCATGGTCTCCGgtattttctcatccactaggttgtgtagtaacaaaatgtatgtaaacataattagtaaacactgagctgaatattccagagagatgactaatgtacctaagattactacaccctgttggtttagaatccactattttagtgctgatgtagccctttttgcagGTTCGGCGCCCTAACCAG
CAACTAGGTCCATTTGATTGTACTAAATATACAAATGCAGCGGACAGTACTGATGTTTATGAAGCTGAATAAGATGCCTGTGGTAGGCATGCTTTTCATGTATGGCAGAAGGGTGATCAGTCTTGTACATAGTTCCTAATTACCAATATGTACGAACTATGAAGTTACCATCTGCGGCAAACAAAGGCATGGAATCTGGC
TTGCAGGGAACTCTTATTATATGCTATGATGCCCTTATGCCAGGGTCTGTTCCAGTAGAATCTCCCATGTTTCTTGTTTTGAAAGATTGTGGTCAAGAACGATACAATAGCAGGCTAAAAGTCTGTTGGTGGAGCAGGATCTGATAGTTTGTTGCAAGTGACCTTAGTGTTGATCATTTTAGATATTTTGTGATTTATTC
TGTTTACAAACTTGTCTTGTAAGTGAACCCATCTCAGGGCTATTGTAATCTGTTTTCATTAGGCTTTCCTTTGTTGGCTGAGAAAATTTCTCAGAAGGATCAATTTTTTGAGCATTTCAGAAATATTTCTGCATTCCAGGCTCCTAAGACAATCCAAATTACACAATGCATTGAACGTTGCAGTTGTGTATGGGTACTCT
TATTTACTTCTGTACATGAGTGCTTGATGGTGCAGAAGAAATGATGGAATTCTTTTCATAAGTAGCGGTTTGGTGCACCAGCTACAGGAAATATATCTGGATGTTAAGAAGGTACTTGCAGCAATTGTTCATGATGTCTTTGTAATTTGGATGTATAAAACTGTAGATAATAAGCTTCCTTTAACACATCTAATTTAAAA
AAGCTGTTAAGTGTAGG

Intron 2 GCAAGTATCT ... TCAGTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	71.14	145	392	323
A2	68.83	127	283	278
A3	67.14	139	297	271
A4	75.81	101	330	303
A5	75.63	34	112	99
A6	68.5	66	138	149
A7	73.7	139	365	414
A8	71.87	109	253	304
A9	72.79	126	339	335
A10	72.72	115	307	306

>TRINITY_DN41575_c0_g3_i7 from sample A8 / Example spliced isoform

ATTTAATTAGAAATGAAGAACACTATGGAGTTTCAAATAGTAATTTAGAAGGAACCATGAGGGATGATAAGGTTGAGAATGGAATCTCTGATTTGGGTTCTAGTTTGCACCAGGAGAACAAAATGAAGCTCGTGTCTTGGCCTGTGAATTTAGAACATTTTCCATCTCCAAGACGAAACTCTGGTTTGTGTTCTACTTTG
CAAGAGAATGAAATGGAGCCCGTGTCTTGGCGTGTAAGGTCAGAACATTTGCCATCCCTAGGCCAAAAGGAAATAGAGAAGGTATATATTGGGTTAGACATTGCTTCAACCACTGGTTCCGTTTCAGTTCAGATTGAAAAGCATGGTGGTGATTCCTGTCAAGTTTTTCAGTTGGAAAATGAAATGGAGACTACATCTCA
GTCTGTGAAATTTGAGCAGTCCCTGGCTACAATAAAAGGAGAAACAGAGAAGGTAACTGAAGAATTCCTCTTTTCTGAAGCCTCTAGTCTTGAGAAGGGTTTGGAGGTAGTTCAAAATGAAGTAAATGATAGAGCTTGCACCAAAGCTTGCCAAGGAAGTATGAATATCCAGAGTGACTATTCAAATGACAAGGTCAAAT
CCATATTCTCTGATGTGATTGGTAGCTTGGAATTCAAAACTGGAATTGTATTTAATCTTAAAGAGAATTCCACAAATTCTTCCAACAATAATCAACATAATACCACCGATACACTTATTACTCCACGTGGGCATTTTGGACAAAGTTGAACAGTTGTGGGAGATTTGCTAGTTCTCTTTCGAGGTATATATGATAGTGGA
ATTGGCATAGTCTATCTTTAAATTTTATTTTATTTTATTTATATATCAATATGAACATGGTTTCTGCAACACTTTTTCTTGATTGAAAACATTTTTTCCTACAAAATTTCTCGTTCATTCTCTTGTGGTGTGTAAG

>TRINITY_DN41575_c0_g3_i1 from sample A8 / Example unspliced isoform:

ATTTAATTAGAAATGAAGAACACTATGGAGTTTCAAATAGTAATTTAGAAGGAACCATGAGGGATGATAAGGTTGAGAATGGAATCTCTGATTTGGGTTCTAGTTTGCACCAGGAGAACAAAATGAAGCTCGTGTCTTGGCCTGTGAATTTAGAACATTTTCCATCTCCAAGACGAAACTCTGGTTTGTGTTCTACTTTG
CAAGAGAATGAAATGGAGCCCGTGTCTTGGCGTGTAAGGTCAGAACATTTGCCATCCCTAGGCCAAAAGGAAATAGAGAAGGTATATATTGGGTTAGACATTGCTTCAACCACTGGTTCCGTTTCAGTTCAGATTGAAAAGCATGGTGGTGATTCCTGTCAAGTTTTTCAGgcaagtatctctatgcaagatgaggattt
acatgataatgttagaaatggaaattctaatttggtttgtcagttgcagTTGGAAAATGAAATGGAGACTACATCTCAGTCTGTGAAATTTGAGCAGTCCCTGGCTACAATAAAAGGAGAAACAGAGAAGGTAACTGAAGAATTCCTCTTTTCTGAAGCCTCTAGTCTTGAGAAGGGTTTGGAGGTAGTTCAAAATGAAG
TAAATGATAGAGCTTGCACCAAAGCTTGCCAAGGAAGTATGAATATCCAGAGTGACTATTCAAATGACAAGGTCAAATCCATATTCTCTGATGTGATTGGTAGCTTGGAATTCAAAACTGGAATTGTATTTAATCTTAAAGAGAATTCCACAAATTCTTCCAACAATAATCAACATAATACCACCGATACACTTATTACT
CCACGTGGGCATTTTGGACAAAGTTGAACAGTTGTGGGAGATTTGCTAGTTCTCTTTCGAGGTATATATGATAGTGGAATTGGCATAGTCTATCTTTAAATTTTATTTTATTTTATTTATATATCAATATGAACATGGTTTCTGCAACACTTTTTCTTGATTGAAAACATTTTTTCCTACAAAATTTCTCGTTCATTCTC
TTGTGGTGTGTAAG