sp|Q7Y0S0|URED_ARATH ----> Urease accessory protein D

Intron 1 GTCATTTGTG ... GATCATGTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	73.33	6	12	21
A2	69.23	2	4	5
A3	76.92	3	11	9
A4	57.89	8	9	13
A5	27.27	4	1	2
A6	48	13	9	15
A7	61.29	18	11	46
A8	50	41	26	56
A9	42.86	22	7	26
A10	58.33	10	6	22

>TRINITY_DN43628_c4_g1_i11 from sample A9 / Example spliced isoform

TCAGCCATTGTTTCTTGACTCGACACAGCTGGAGCAAGGTGCTGGCTATTCTGTTGTGGATCGCATGCAATCTTTTCACGTGATAGCAATGCTGGTAGTTTTGGGCTTGAACAATAATATGAAAGATTGCAAGTGTTGTGAAGCAGTCTACCTCATTCTTTTTTACATGACTGACCAAAGTTGGAACCCACACGGAAGCA
TATAATGGACAAAATTAGGGAGAGAAATTCGGAGTTGTTTGGCGGTCCATTACAGAACTCAAATACTCATGCTAAGAAAGCAAAGCTCGACGAAAGCAAAGCATCAGAAGCAGTTCTACTTGCCTCATGTAGTGCATTTGG

>TRINITY_DN43628_c4_g1_i9 from sample A9 / Example unspliced isoform:

TGGGTTTTTTTGTGTTACAGCCTGGGGTGGAAGAGATGTCAGATGTAATGTTAATAAACTAAAATAAGAAAAGGTTGACATCCAAACATGTAATGTTTCAAAAGAAAAGATTATTATAATGTAAAATAAATAAACTAGCATAGATCCATCTTAACCCTAAATTTTCAGTCATTTTACATTTGCTTAACAAAGGAACAGGG
AATATAATGGAAATTTGATTCCCACCCAGTGATCAGTCTTCTACAAGAGATTCTTTCACTTTCACCCTATTCAAAGAGGGGTTGAAACTTTATTGAAGCAAAAGAGTGGAAACAATAGCTTGCTGTCATGCTCACAGTACTTTGTCCATGTGCAGGGATTTTGATTGCAGTATTACATCTCTACATTTGCCATAATCCTT
ATGCTAACCATTTTGTCTTTGTTTTGTTTTGATATTTTCAGCTTGAACAATAATATGAAAGATTGCAAGTGTTGTGAAGCAGTCTACCTCATTCTTTTTTACATGACTGgtcatttgtgaactctaattactctctttgtttcttttaaatgtctttaccattttatcttttggtcaaatatttattgcaaagaaacata
tgccttttccctttatacatttggcttcggtaattttcctccaactttcaatgtatttttcaaatgctctcttctttgaaaggcagttcatccgctgacaatatgctatggatctaatattgctttggttaaagatgtgaccagatatattagggcacgttctaaccatggctgtgtaaagctttgttcactttgatttt
ttaatgcatgaagttatttcctcgaaaagagaagaagtccatccataacttgccgatttgatcatgtagACCAAAGTTGGAACCCACACGGAAGCATATAATGGACAAAATTAGGGAGAGAAATTCGGAGTTGTTTGGCGGTCCATTACAGAACTCAAATACTCATGCTAAGAAAGCAAAGCTCGACGAAAGCAAAGCAT
CAGAAGCAGTTCTACTTGCCTCATGTAGTGCATTTGGCCCTATGGATATTGGTTTGGTGGTTCGTATAGTAGCAACTACCACTGAATCAGTTTACAAATTCTTCAGAGAACATCTTACATCTATTGGGCCTTTTATAGGGACATGCCCATACGGGGGAAAGACGATTACCTAATGCATATTTTAACAAGTACAAGAACGC
ATGGCAAAATTGTAAAAGATACTACACAAATAAAATGTTTTTGTGTTGTCTTTTGAAATTGTAATATGCCTAACTTAAATAGAAAAGCTTTGGAGAATAATAAGATGAACATGGATCTAATAAAGTGAACATTTTGGATGCAGAATGAGAATGATAGGCATTCCTTGCAGTGATGCAAATACATCTAACAGTTTATGAAA
GCTATATTTTGGTTATGATGCTGTGATTGGGGGTATTTATTGTGCCAGGTTTGTTTGTGACTTAAACGTGGTCCTAATTGCATGAAATAGC

Intron 2 GTAAGGGATT ... TCTTTTTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	42.86	12	10	8
A2	33.33	10	3	7
A3	29.73	13	6	5
A4	48.39	8	14	1
A5	28.57	5	3	1
A6	28.3	19	9	6
A7	36.32	71.1	59	22.1
A8	33.06	81.6	47	33.6
A9	24.24	50	25	7
A10	29.02	54.2	34.9	9.4

>TRINITY_DN117549_c1_g1_i4 from sample A3 / Example spliced isoform

AGCTGGAGCAAGGTGCTGGCTATTCTGTTGTGGATCGCATGCAATCTTTTCACGTGCTAGCAATGCTGGTAGTTTTGGGACCAAAGTTGGAACCCACACGGAAGCATATAATGGACAAAATTAGGGAGAGAAATTCGGAGTTGTTTGGCGGTCCATTACAGAACTCAAATACTCATGCTAAGAAAGCAAAGCTCGACGAA
AGCAAAGCATCAGAAGCAGTTCTACTTGCCTCATGTAGTGCATTTGGTCCTATGGATATTGGTTTGGTGGTTCGTATAGTAGCAACTACCACTGAATCAGTTTACAAATTCTTCAGAGAACATCTTACATCTATTGGGCCTTTTATTGGGACATGCCCATACGGGGGAAAGACGATTACCTAATGCATATTTTAACAAGT
ACAAGAACGCATGGCAAAATTGTAAAAGATACTACACAAATAAAATGTTTTTGTGTTGTCTTTTGAAATTGTAATATGCCTAACTTAAATAGAAAAGCTTTGGAGAATAATAAGATGAACATGGATCTAATAAAGTGAACATTTTGGATGCAGAATGAGAATGATAGGCATTCCTTGCAGTGATGCAAATACATCTAACA
GTTTATGAAAGCTATATTTTGGTTATGATGCTGTGATTGGGGGTATTTATTGTGCCAGGTTTGTTTGTGACTTAAACGTGGTCCTAATTGCATGAAATAGCCTACTTA

>TRINITY_DN117549_c1_g1_i3 from sample A3 / Example unspliced isoform:

AAAGATTATTATAATGTAAAACAAATAAACTAGCATAGATCCATCTTAACCCTAAATTTTCAGTCATTTTATATTTACTTAACAAAGGAACAGGGAATATAATGGAAATTTGATTCTCACCCAGTGATCAGTCTTCTACCAGAGATTCTTTCACTTTCACCCTATTCAAAGAGGGGTTGAAAATTTATTGAAGCAAAAGA
GTAGAAACAATAGCTTGCTGTCATGCTCACAGTACTTTGTCCATGTGCAGGGATTTTGATTGCAGTATTACATCTCTACATTTGCCATAATCCTTATGCTAACCATTTTGTCTTTGTTTTGTTTTGATATTTTCAGCTTGAACAATAATATGAAAGATTGCAAGTGTTGTGAAGCAGTCTACCTCATTCTTTTTTACATG
ACTGGTCATTTGTGAACTCTAATTACTCTCTTTGTTTCTTTTAAATGTCTTTACCATTTTATCTTTTGGTCAAATATTTATTGCAAAGAAACATATGCCTTTTCCCTTTATACGTTTGGCTTCAAATGCTCTCTTCTTTGAAAGGCAGTTCATCCGCTGACAATATGCTATGGATCTAATATTGCTTTGGTTAAAGATGT
GACCAGATATATTAGGGCACGTTCTAACCATGGCTGTGTAAAGCTTTGTTCACTTTGATTTTTTAATGCATGAAGTTATTTCCTCGAAAAGAGAAGAAGTCCATCCATAACTTGCCGATTTGATCATGTAGACCAAAGTTGGAACCCACACGGAAGCATATAATGGACAAAATTAGGGAGAGAAATTCGGAGTTGTTTGG
CGGTCCATTACAGAACTCAAATACTCATGCTAAGAAAGCAAAGCTCGACGAAAGCAAAGCATCAGAAGCAGTTCTACTTGCCTCATGTAGTGCATTTGGTCCTATGgtaagggattattgcattgtgaagtaattcaaaagaggtttgtaaacggaagtccatattttataatcagtgcctgctgcatcagaacttttca
taaaccccaattcaaacaatcatctaaaaaacgtttctttttcagGATATTGGTTTGGTGGTTCGTATAGTAGCAACTACCACTGAATCAGTTTACAAATTCTTCAGAGAACATCTTACATCTATTGGGCCTTTTATTGGGACATGCCCATACGGGGGAAAGACGATTACCTAATGCATATTTTAACAAGTACAAGAACG
CATGGCAAAATTGTAAAAGATACTACACAAATAAAATGTTTTTGTGTTGTCTTTTGAAATTGTAATATGCCTAACTTAAATAGAAAAGCTTTGGAGAATAATAAGATGAACATGGATCTAATAAAGTGAACATTTTGGATGCAGAATGAGAATGATAGGCATTCCTTGCAGTGATGCAAATACATCTAACAGTTTATGAA
AGCTATATTTTGGTTATGATGCTGTGATTGGGGGTATTTATTGTGCCAGGTTTGTTTGTGACTTAAACGTGGTCCTAATTGCATGAAATAGCCTACTTA

Intron 3 GTCATTTGTG ... GATCATGTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	73.33	6	12	21
A2	69.23	2	4	5
A3	76.92	3	11	9
A4	58.97	8	9	14
A5	27.27	4	1	2
A6	48	13	9	15
A7	61.29	18	11	46
A8	50	41	26	56
A9	42.86	22	7	26
A10	58.33	10	6	22

>TRINITY_DN43628_c4_g1_i11 from sample A9 / Example spliced isoform

TCAGCCATTGTTTCTTGACTCGACACAGCTGGAGCAAGGTGCTGGCTATTCTGTTGTGGATCGCATGCAATCTTTTCACGTGATAGCAATGCTGGTAGTTTTGGGCTTGAACAATAATATGAAAGATTGCAAGTGTTGTGAAGCAGTCTACCTCATTCTTTTTTACATGACTGACCAAAGTTGGAACCCACACGGAAGCA
TATAATGGACAAAATTAGGGAGAGAAATTCGGAGTTGTTTGGCGGTCCATTACAGAACTCAAATACTCATGCTAAGAAAGCAAAGCTCGACGAAAGCAAAGCATCAGAAGCAGTTCTACTTGCCTCATGTAGTGCATTTGG

>TRINITY_DN43628_c4_g1_i2 from sample A9 / Example unspliced isoform:

TGGGTTTTTTTGTGTTACAGCCTGGGGTGGAAGAGATGTCAGATGTAATGTTAATAAACTAAAATAAGAAAAGGTTGACATCCAAACATGTAATGTTTCAAAAGAAAAGATTATTATAATGTAAAATAAATAAACTAGCATAGATCCATCTTAACCCTAAATTTTCAGTCATTTTATATTTACTTAACAAAGGAACAGGG
AATATAATGGAAATTTGATTCCCACCCAGTGATCAGTCTTCTACAAGAGATTCTTTCACTTTCACCCTATTCAAAGAGGGGTTGAAACTTTATTGAAGCAAAAGAGTGGAAACAATAGCTTGCTGTCATGCTCACAGTACTTTGTCCATGTGCAGGGATTTTGATTGCAGTATTACATCTCTACATTTGCCATAATCCTT
ATGCTAACCATTTTGTCTTTGTTTTGTTTTGATATTTTCAGCTTGAACAATAATATGAAAGATTGCAAGTGTTGTGAAGCAGTCTACCTCATTCTTTTTTACATGACTGgtcatttgtgaactctaattactctctttgtttcttttaaatgtctttaccattttatcttttggtcaaatatttattgcaaagaaacata
tgccttttccctttatacgtttggcttcaaatgctctcttctttgaaaggcagttcatccgctgacaatatgctatggatctaatattgctttggttaaagatgtgaccagatatattagggcacgttctaaccatggctgtgtaaagctttgttcactttgattttttaatgcatgaagttatttcctcgaaaagagaa
gaagtccatccataacttgccgatttgatcatgtagACCAAAGTTGGAACCCACACGGAAGCATATAATGGACAAAATTAGGGAGAGAAATTCGGAGTTGTTTGGCGGTCCATTACAGAACTCAAATACTCATGCTAAGAAAGCAAAGCTCGACGAAAGCAAAGCATCAGAAGCAGTTCTACTTGCCTCATGTAGTGCAT
TTGGCCCTATGGATATTGGTTTGGTGGTTCGTATAGTAGCAACTACCACTGAATCAGTTTACAAATTCTTCAGAGAACATCTTACATCTATTGGGCCTTTTATAGGGACATGCCCATACGGGGGAAAGACGATTACCTAATGCATATTTTAACAAGTACAAGAACGCATGGCAAAATTGTAAAAGATACTACACAAATAA
AATGTTTTTGTGTTGTCTTTTGAAATTGTAATATGCCTAACTTAAATAGAAAAGCTTTGGAGAATAATAAGATGAACATGGATCTAATAAAGTGAACATTTTGGATGCAGAATGAGAATGATAGGCATTCCTTGCAGTGATGCAAATACATCTAACAGTTTATGAAAGCTATATTTTGGTTATGATGCTGTGATTGGGGG
TATTTATTGTGCCAGGTTTGTTTGTGACTTAAACGTGGTCCTAATTGCATGAAATAGC