sp|Q8RWA2|MED9_ARATH ----> Mediator of RNA polymerase II transcription subunit 9

Intron 1 GTACCTAGTG ... TTGTTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	50	9	1	9
A2	50	1	0	1
A3	55.56	4	2	5
A4	-1	0	0	0
A5	40	3	0	2
A6	37.25	32	1	19
A7	30.95	29	1	13
A8	35.71	36	0	20
A9	17.95	32	0	7
A10	45.83	13	0	11

>TRINITY_DN35046_c0_g2_i4 from sample A7 / Example spliced isoform

TTGATTGAGGGCTTGGCAAGTGCAGTTGAAGGAGGAGTTCGAGATCAGAATTCAGATATGCTGGTTAATGAGTTGACAGCACAGTTTGAGAAATGCCAGCAATCACTGAATGCTCTTGCAGGACCCAATGATACTATGACTGTCCGTGGCCAAAAGCAAAAGTTGGAGGAGTATGAAAAGCAACTAAACAATAGGAGGTT
TGTCTGTCTATTCTGTGTTAGCGAGAATTGGTCATGAAATACAAAAGCATAGTGGAAAACTATGTCGGATCATCTTGATTTTAAAAGAAGTTTCAGTCTGACTAAAGCATGAGAGGATAGAGAACCATTGGCTGTTTCAGCTTCTATCACGTTCATGATGTATAACCTATTTTCAGTTACCATGTTAGGCTGATTCTCAT
TCTTTTTCTTTATAGATACATTGAGAAAAGGGTGTGAGCTATACTTAGAATTTGTGCGGATATTAATGCAGATCACTAGTGAGCAATTACAGTTAGTTGTTAGAAGCTTGTCAATGGATTTTTGCAGCTTTACTTGAGAGGCTGGTTATTGATAATTTAGTTTTACACAACACTGCAAAACAATACATGTGGGCACCATT
TCTTTAGGAAATGGGGTGGTACGGAAACTCTGTTTCATGGGCATTTAATGTGCCCATGGCAGCATTGAAGAAAATGGCTGACTTGAGAAACCACCATAAAGGTAATAAAAAAAGATATGGATGTAATTTAATTTATAACATTGATACAGTATGATGATGATCACTCTCCATTGAGACCAAG

>TRINITY_DN35046_c0_g2_i2 from sample A7 / Example unspliced isoform:

TTGATTGAGGGCTTGGCAAGTGCAGTTGAAGGAGGAGTTCGAGATCAGAATTCAGATATGCTGGTTAATGAGTTGACAGCACAGTTTGAGAAATGCCAGCAATCACTGAATGCTCTTGCAGGACCCAATGATACTATGACTGTCCGTGGCCAAAAGCAAAAGTTGGAGGAGTATGAAAAGCAACTAAACAATAGGAGGTT
TGTCTGTCTATTCTGTGTTAGCGgtacctagtgtaaattaacaagtactactgtctttttgctattaatatttgttagctcttgttataaattgtttctttcttgtatctcttcatgagtgatgtttttgtttgtttgcagAGAATTGGTCATGAAATACAAAAGCATAGTGGAAAACTATGTCGGATCATCTTGATTTT
AAAAGAAGTTTCAGTCTGACTAAAGCATGAGAGGATAGAGAACCATTGGCTGTTTCAGCTTCTATCACGTTCATGATGTATAACCTATTTTCAGTTACCATGTTAGGCTGATTCTCATTCTTTTTCTTTATAGATACATTGAGAAAAGGGTGTGAGCTATACTTAGAATTTGTGCGGATATTAATGCAGATCACTAGTGA
GCAATTACAGTTAGTTGTTAGAAGCTTGTCAATGGATTTTTGCAGCTTTACTTGAGAGGCTGGTTATTGATAATTTAGTTTTACACAACACTGCAAAACAATACATGTGGGCACCATTTCTTTAGGAAATGGGGTGGTACGGAAACTCTGTTTCATGGGCATTTAATGTGCCCATGGCAGCATTGAAGAAAATGGCTGAC
TTGAGAAACCACCATAAAGGTAATAAAAAAAGATATGGATGTAATTTAATTTATAACATTGATACAGTATGATGATGATCACTCTCCATTGAGACCAAG

Intron 2 GTTTGTCTGT ... TTGTTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	39.13	14	2	9
A2	33.33	2	0.7	1
A3	26.32	14	3.7	5
A4	-1	0	0	0
A5	40	3	0	2
A6	29.69	45	4	19
A7	27.47	34.3	3	13
A8	26.67	55	4.7	20
A9	12.43	49.3	8.7	7
A10	32.35	23	4.7	11

>TRINITY_DN30758_c0_g1_i2 from sample A6 / Example spliced isoform

GGGGAGTGTGATTATTTTTCTGTCTGTACGTGCCTTCTGCGTTTTTTCTCTTCTCTGGCCAACGTAATGGATCCATCAATGATGGCCTGGATGGGCATGCAGAGTCAGCAACAGAATCAAAATCAGAACCAACTTCAAATACAACAGCACCAGCCATCAGCTTCTCAGTTCAATCTCTTTCCCTTGATTGAGGGCTTGGC
AAGTGCAGTTGAAGGAGGAGTTCGAGATCAGAATTCAGATATGCTGGTTAATGAGTTGACAGCACAGTTTGAGAAATGCCAGCAATCACTGAATGCTCTTGCAGGACCCAATGATACTATGACTGTCCGTGGCCAAAAGCAAAAGTTGGAGGAGTATGAAAAGCAACTAAACAATAGGAGAGAATTGGTCATGAAATACA
AAAGCATAGTGGAAAACTATGTCGGATCATCTTGATTTTAAAAGAAGTTTCAGTCTGACTAAAGCATGAGAGGATAGAGAACCATTGGCTGTTTCAGCTTCTATCACGTTCATGATGTATAACCTATTTTCAGTTACCATGTTAGGCTGATTCTCATTCTTTTTCTTTATAGATACATTGAGAAAAGGGTGTGAGCTATA
CTTAGAATTTGTGCGGATATTAATGCAGATCACTAGTGAGCAATTACAGTTAGTTGTTAGAAGCTTGTCAATGGATTTTTGCAGCTTTACTTGAGAGGCTGGTTATTGATAATTTAGTTTTACACAACACTGCAAAACAATACATGTGGGCACCATTTCTTTAGGAAATGGGGTGGTACGGAAACTCTGTTTCATGGGCA
TTTAATGTGCCCATGGCAGCATTGAAGAAAATGGCTGACTTGAGAAACCACCATAAAGGTAATAAAAAAAGATATGGATGTAATTTAATTTATAACATTGATACAGTATGATGATGATCACTCTCCATTGAGACCAACATTGGTCTATTAAATCTTTGAGAAAATAAACATAGG

>TRINITY_DN30758_c0_g1_i3 from sample A6 / Example unspliced isoform:

GAAAAGCAACTAAACAATAGGAGgtttgtctgtctattctgtgttagcggtacctagtgtaaattaacaagtactactgtctttttgctattaatatttgttagctcttgttataaattgtttctttcttgtatctcttcatgagtgatgtttttgtttgtttgcagAGAATTGGTCATGAAATACAAAAGCATAGTGGA
AAACTATGTCGGATCATCTTGATTTTAAAAGAAGTTTCAGTCTGACTAAAGCATGAGAGGATAGAGAACCATTGGCTGTTTCAGCTTCTATCACGTTCATGATGTATAACCTATTTTCAGTTACCATGTTAGGCTGATTCTCATTCTTTTTCTTTATAGATACATTGAGAAAAGGGTGTGAGCTATACTTAGAATTTGTG
CGGATATTAATGCAGATCACTAGTGAGCAATTACAGTTAGTTGTTAGAAGCTTGTCAATGGATTTTTGCAGCTTTACTTGAGAGGCTGGTTATTGATAATTTAGTTTTACACAACACTGCAAAACAATACATGTGGGCACCATTTCTTTAGGAAATGGGGTGGTACGGAAACTCTGTTTCATGGGCATTTAATGTGCCCA
TGGCAGCATTGAAGAAAATGGCTGACTTGAGAAACCACCATAAAGGTAATAAAAAAAGATATGGATGTAATTTAATTTATAACATTGATACAGTATGATGATGATCACTCTCCATTGAGACCAACATTGGTCTATTAAATCTTTGAGAAAATAAACATAGG