sp|O80813|YC20L_ARATH ----> Ycf20-like protein

Intron 1 GTGTGCCTTC ... GTTGCTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	64.91	10	26	11
A2	63.64	4	10	4
A3	60	12	21	15
A4	72.73	3	10	6
A5	84.62	1	7	4
A6	66.04	36	79	61
A7	53.51	43	60	39
A8	58.88	44	72	54
A9	60.77	41	70	57
A10	49.13	44	50	35

>TRINITY_DN120114_c0_g1_i5 from sample A1 / Example spliced isoform

CAGGCTACTTCGTTGGTTTCGCGGGTTCAGAACATGGTTTTCTTTGGTGAGGGTTTCAATGGCGTCTTAGGGTTTGAAAATGAACTTCCCCTCTACCAAATTCCTAAACTCATGGAGGCCAAACCACAGAAAACAATACTATGGCAAATGCCGTGAGATTATTGAAGCCACTACTACAACAAAATTTTCCTTTCAACAAC
GAATAAACCCAGTTTCATTTTAGGTTTATACACTAAATTGCCATATATATGAAGTGCAATTTTGTAAATTTGACGTGCAATCCCCGTTCTCGCTTCATTTCTGCGATTAGGATGCAACAGGGACTGATCGAATTTAATATATTTCAAGGAGTTGATAATTCTAGTGCTTGATGGAGCTAAGAGGGCGAGGAATTTGACAA
TCTCCTTCAAGGTTCGAATCGCAGGGAAGACACTGGTGTTCTTATCTCCTTGGTATGGCTTTGGAAGGGCTCCACGTTCCATCTCTGTCAAATAGTCATTTAAGCGCAAAAGCATTAGCAACAGTTGCTGTTCCATGTAATGTTGGCAGGAGCTTGGCATTTGGTCAAGGGAGATTTCGGTGTATACGTTGCATCCATAA
CCGAGGTGCATCTGTATCTTTGAACATCAAAAGAAAATGGAAGCACAAGTCTTTGGTCATAAAGTGTAGGAGAAATAACGGGAGTTCTGATAAAAGTGAGACTCCACAAAGTGATGGGCCTTTAAGACGATCTGGTTTAGGAAGAACACGCCTCATTAGAGTTGTTCAGGAGGTTCAGAACAAAATAACAAAAAAATTCA
AAGAGCTGCGAAAGAACTTTCCGATGAAAGTATTGTGTCTGCTTTTAGGATTTTACTGTGCAGGTCTTGTTGCTACAAGTCTAGCTCAGACAGGGGATTGGGATATTTTGTCTGCAGGTCTGGCAGTAGTTATAATCGAAGCAATTGGTGCATTAATGTATCGGGCATATCCATTAATAGAAAGGTTACATTTTTTTGTC
ATAATGGTCAATTATTGGAAGGCAGGTCTTTCTTTAGGTCTATTTTTGGATGCATTCAAGTATGAAATGGATGTAAATTCAGTGCTATGTGATTTATTTTATCTTGAAATAAATCTATTTTCAGTGCTTTGGTGGTAGGGCTTTCATCTAGAGTTTCCATGGCCTTTCATTTGGACTTTTCACCTTCTGGATGGAAGGAA
AACAAGAAGTAAAAAGCAATCAGTAGAGATACAAGAGAAAGCACACCATAATTGTGTGTAAGTGAATGTTGACCGTTCTCTCCTGCACATGCTCTGGTTCGAGAGACATTTTTTTGTATTATATTTAGAACAGTTTGACATCCCTGTTGGAATTCTCCATATTACTGTCATTAAGTTTGAACCCAGTTAAATATATGAGC
AGTAATCACAAACTGCATTTCAGCACTAGCTACCGATAATTAAGGGGAGG

>TRINITY_DN120114_c0_g1_i1 from sample A1 / Example unspliced isoform:

CAGGCTACTTCGTTGGTTTCGCGGGTTCAGAACATGGTTTTCTTTGGTGAGGGTTTCAATGGCGTCTTAGGGTTTGAAAATGAACTTCCCCTCTACCAAATTCCTAAACTCATGGAGGCCAAACCACAGAAAACAATACTATGGCAAATGCCGTGAGATTATTGAAGCCACTACTACAACAAAATTTTCCTTTCAACAAC
GAATAAACCCAGTTTCATTTTAGGTTTATACACTAAATTGCCATATATATGAAGTGCAATTTTGTAAATTTGACGTGCAATCCCCGTTCTCGCTTCATTTCTGCGATTAGGATGCAACAGGGACTGATCGAATTTAATATATTTCAAGGAGTTGATAATTCTAGTGCTTGATGGAGCTAAGAGGGCGAGGAATTTGACAA
TCTCCTTCAAGGTTCGAATCGCAGGGAAGACACTGGTGTTCTTATCTCCTTGGTATGGCTTTGGAAGGGCTCCACGTTCCATCTCTGTCAAATAGTCATTTAAGCGCAAAAGCATTAGCAACAGTTGCTGTTCCATGTAATGTTGGCAGGAGCTTGGCATTTGGTCAAGGGAGATTTCGGTGTATACGTTGCATCCATAA
CCGAGGTGCATCTGTATCTTTGAACATCAAAAGAAAATGgtgtgccttcaactctgttttttcttcagccttatccctgcttccttttctcagaagaggataacactacaaaatatcaaggaaatactgtggtcatctttgtattaggtcacgcttctgatggcccatctttgccaatacctaatttgagtttgccagtg
atgcaaattactttgcatgttgctgcagGAAGCACAAGTCTTTGGTCATAAAGTGTAGGAGAAATAACGGGAGTTCTGATAAAAGTGAGACTCCACAAAGTGATGGGCCTTTAAGACGATCTGGTTTAGGAAGAACACGCCTCATTAGAGTTGTTCAGGAGGTTCAGAACAAAATAACAAAAAAATTCAAAGAGCTGCGA
AAGAACTTTCCGATGAAAGTATTGTGTCTGCTTTTAGGATTTTACTGTGCAGGTCTTGTTGCTACAAGTCTAGCTCAGACAGGGGATTGGGATATTTTGTCTGCAGGTCTGGCAGTAGTTATAATCGAAGCAATTGGTGCATTAATGTATCGGGCATATCCATTAATAGAAAGGTTACATTTTTTTGTCATAATGGTCAA
TTATTGGAAGGCAGGTCTTTCTTTAGGTCTATTTTTGGATGCATTCAAGTATGAAATGGATGTAAATTCAGTGCTATGTGATTTATTTTATCTTGAAATAAATCTATTTTCAGTGCTTTGGTGGTAGGGCTTTCATCTAGAGTTTCCATGGCCTTTCATTTGGACTTTTCACCTTCTGGATGGAAGGAAAACAAGAAGTA
AAAAGCAATCAGTAGAGATACAAGAGAAAGCACACCATAATTGTGTGTAAGTGAATGTTGACCGTTCTCTCCTGCACATGCTCTGGTTCGAGAGACATTTTTTTGTATTATATTTAGAACAGTTTGACATCCCTGTTGGAATTCTCCATATTACTGTCATTAAGTTTGAACCCAGTTAAATATATGAGCAGTAATCACAA
ACTGCATTTCAGCACTAGCTACCGATAATTAAGGGGAGG

Intron 2 GTTTATACAC ... ATATTTCAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	66.75	10.7	26	16.7
A2	68.25	12.6	29	23.7
A3	73.33	6	21	12
A4	69.55	7	17	14.3
A5	56.25	7	11	7
A6	66.84	28.5	60	53
A7	66.96	34.3	81	54.5
A8	70.86	15.9	45	31.3
A9	68.64	29	76	47.2
A10	71.24	29.7	103	42.8

>TRINITY_DN120114_c0_g1_i6 from sample A1 / Example spliced isoform

CAGGCTACTTCGTTGGTTTCGCGGGTTCAGAACATGGTTTTCTTTGGTGAGGGTTTCAATGGCGTCTTAGGGTTTGAAAATGAACTTCCCCTCTACCAAATTCCTAAACTCATGGAGGCCAAACCACAGAAAACAATACTATGGCAAATGCCGTGAGATTATTGAAGCCACTACTACAACAAAATTTTCCTTTCAACAAC
GAATAAACCCAGTTTCATTTTAGGAGTTGATAATTCTAGTGCTTGATGGAGCTAAGAGGGCGAGGAATTTGACAATCTCCTTCAAGGTTCGAATCGCAGGGAAGACACTG

>TRINITY_DN120114_c0_g1_i1 from sample A1 / Example unspliced isoform:

CAGGCTACTTCGTTGGTTTCGCGGGTTCAGAACATGGTTTTCTTTGGTGAGGGTTTCAATGGCGTCTTAGGGTTTGAAAATGAACTTCCCCTCTACCAAATTCCTAAACTCATGGAGGCCAAACCACAGAAAACAATACTATGGCAAATGCCGTGAGATTATTGAAGCCACTACTACAACAAAATTTTCCTTTCAACAAC
GAATAAACCCAGTTTCATTTTAGgtttatacactaaattgccatatatatgaagtgcaattttgtaaatttgacgtgcaatccccgttctcgcttcatttctgcgattaggatgcaacagggactgatcgaatttaatatatttcaagGAGTTGATAATTCTAGTGCTTGATGGAGCTAAGAGGGCGAGGAATTTGACAA
TCTCCTTCAAGGTTCGAATCGCAGGGAAGACACTGGTGTTCTTATCTCCTTGGTATGGCTTTGGAAGGGCTCCACGTTCCATCTCTGTCAAATAGTCATTTAAGCGCAAAAGCATTAGCAACAGTTGCTGTTCCATGTAATGTTGGCAGGAGCTTGGCATTTGGTCAAGGGAGATTTCGGTGTATACGTTGCATCCATAA
CCGAGGTGCATCTGTATCTTTGAACATCAAAAGAAAATGGTGTGCCTTCAACTCTGTTTTTTCTTCAGCCTTATCCCTGCTTCCTTTTCTCAGAAGAGGATAACACTACAAAATATCAAGGAAATACTGTGGTCATCTTTGTATTAGGTCACGCTTCTGATGGCCCATCTTTGCCAATACCTAATTTGAGTTTGCCAGTG
ATGCAAATTACTTTGCATGTTGCTGCAGGAAGCACAAGTCTTTGGTCATAAAGTGTAGGAGAAATAACGGGAGTTCTGATAAAAGTGAGACTCCACAAAGTGATGGGCCTTTAAGACGATCTGGTTTAGGAAGAACACGCCTCATTAGAGTTGTTCAGGAGGTTCAGAACAAAATAACAAAAAAATTCAAAGAGCTGCGA
AAGAACTTTCCGATGAAAGTATTGTGTCTGCTTTTAGGATTTTACTGTGCAGGTCTTGTTGCTACAAGTCTAGCTCAGACAGGGGATTGGGATATTTTGTCTGCAGGTCTGGCAGTAGTTATAATCGAAGCAATTGGTGCATTAATGTATCGGGCATATCCATTAATAGAAAGGTTACATTTTTTTGTCATAATGGTCAA
TTATTGGAAGGCAGGTCTTTCTTTAGGTCTATTTTTGGATGCATTCAAGTATGAAATGGATGTAAATTCAGTGCTATGTGATTTATTTTATCTTGAAATAAATCTATTTTCAGTGCTTTGGTGGTAGGGCTTTCATCTAGAGTTTCCATGGCCTTTCATTTGGACTTTTCACCTTCTGGATGGAAGGAAAACAAGAAGTA
AAAAGCAATCAGTAGAGATACAAGAGAAAGCACACCATAATTGTGTGTAAGTGAATGTTGACCGTTCTCTCCTGCACATGCTCTGGTTCGAGAGACATTTTTTTGTATTATATTTAGAACAGTTTGACATCCCTGTTGGAATTCTCCATATTACTGTCATTAAGTTTGAACCCAGTTAAATATATGAGCAGTAATCACAA
ACTGCATTTCAGCACTAGCTACCGATAATTAAGGGGAGG