sp|Q8LC83|RS242_ARATH ----> Small ribosomal subunit protein eS24y

Intron 1 GTAATATTGT ... GGTTGTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	16.44	424	19.7	78.8
A2	17.68	304.3	13.8	61.5
A3	22.04	344.7	18.7	91
A4	17.94	159.2	15	33
A5	17.47	115.3	9	22.8
A6	20.95	1937.7	45.7	479.8
A7	19.68	3244.8	79.5	742
A8	19.95	4262.5	102.8	985.3
A9	19.44	3696.3	82.8	834
A10	16.63	2157.5	48.7	404

>TRINITY_DN123166_c8_g1_i2 from sample A1 / Example spliced isoform

CATAGAACATTTTTATTAACTTTACAATTTTTGTCTATGAGAGACGCCCTGTCAGCAAAACTCTACATCTGACGTGGCAGTTAATTGCCAACACGACCTCCAACGAGAGTGGTCAAATATTGGTGGCTTTCCTCTAATAAGACGATAGGTTTAGGGTTTCGGCAAAGCATAGGAAGACAGTGTTCTCTGAGCTCACACCG
AGGAGAAGAGCCGAGGCCGCGAGATCCGTGAGGAGGAAGGAGCCGAATACCATCAACAATGGCGGATGCTAAAGCCGTTACAGTCAGAACTCGCAAGTTCATGACTAACAGGCTTCTATCAAGGAAGCAATTCGTGATTGATGTTCTTCATCCTGGTAGGCCAAATGTATCCAAGGCCGAGCTGAAGGAGAAGCTGGCAA
AGCTGTACGAGGTCAGAGATCCGCAGACAATATTTGTTTTTGGGTTCAGAACCCAATTCGGAGGTGGAAAATCTTCGGGGTTTGGCTTGATCTATGACAACTTGGAGCATGCAAAGAAATACGAACCCAAGTACAGGCTTATCAGGAATGGACTCGCAACCAAGGTAGAGAAATCCAGGAAGCAAATGAAGGAAAGGAAG
AACAGAGCTAAGAAAATTCGTGGAATCAAGAAGACTAAGGCTGGAGATGCAGCTAAGAAGAAGAAATGATGGGTTTTTGCAATTAACTATAATAGGAGAGTTTTTATACATCGCTGGTTCTTAGGCATGGAATTTTGTGCAAGACTTTTTGTTGGCTGGATTATTATCCATACAAGGTTGAGCATATCGATGACTGTATT
GCAAATCACTAGAATTTATTTATTAAGCAAATTTTTGTAATGTTGCAATCTGCATTTTGTTACTTATTTAAAATTTAAAGAAAACTGTGGTATAATTGTCTTGGGAGATTTAAATGTGTTGACTTGTATTTCAATCGTCTACGTTTCTATGTGTTTGAGATATAAT

>TRINITY_DN123166_c8_g1_i1 from sample A1 / Example unspliced isoform:

AATGGTTTGGATTTACATTGATAATATTTTTAGTAAACCAGTCCAGTTTGACCCTCAGCCATTCCGAGTTTGATTCCAATAATTTTATTATGAATTTGTGTCAGAAAGTTTCCCATCCACCTGTTTAAAAGTCTTTCTTTTGTGGACCCAGTGTTGGTTTTCATCTCAAGGTGATGAAAATCCATTATCTTATAAGGTGG
TTGTAATTGGCATACCTTGAGTTTGTGTGCAGAATGGACTCGCAACCAAGGTAGAGAAATCCAGGAAGCAAATGAAGGAAAGGAAGAACAGAGCTAAGAAAATTCGTGGAATCAAGAAGgtaatattgtgttattaaggtgctttgctcactagcaatataacacgtgaaacctctgtatttattgcattctcggttttt
ggttgtgcagACTAAGGCTGGAGATGCAGCTAAGAAGAAGAAATGATGGGTTTTTGCAATTAACTATAATAGGAGAGTTTTTATACATCGCTGGTTCTTAGGCATGGAATTTTGTGCAAGACTTTTTGTTGGCTGGATTATTATCCATACAAGGTTGAGCATATCGATGACTGTATTGCAAATCACTAGAATTTATTTAT
TAAGCAAATTTTTGTAATGTTGCAATCTGCATTTTGTTACTTATTTAAAATTTAAAGAAAACTGTGGTATAATTGTCTTGGGAGATTTAAATGTGTTGACTTGTATTTCAATCGTCTACGTTTCTATGTGTTTGAGATATAAT

Intron 2 GTAAGTATTT ... CTTGTCACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	17.8	702	152	4
A2	18.1	706	156	10
A3	16.49	856	169	2
A4	19.85	416	103	6
A5	18.26	300	67	2
A6	16.36	2342	458	0
A7	17	4833	990	0
A8	15.45	4078	745	0
A9	17.49	4995	1059	3
A10	15.95	4316	819	0

>TRINITY_DN110672_c4_g6_i1 from sample A5 / Example spliced isoform

AGGGTTTCGGCAAAGCATAGGAAGACAGTGTTCTCTGAGCTCACACCGAGGAGAAGAGCCGAGGCCGCGAGATCCGTGAGGAGGAAGGAGCCGAATACCATCAACAATGGCGGATGCTAAAGCCGTTACAGTCAGAACTCGCAAGTTCATGACTAACAGGCTTCTATCAAGGAAGCAATTCGTGATTGATGTTCTTCATC
CTGGTAGGCCAAATGTATCCAAGGCCGAGCTGAAGGAGAAGCTGGCAAAGCTGTACGAGGTCAGAGATCCGCAGACAATATTTGTTTTTGGGTTCAGAACCCAATTCGGAGGTGGAAAATCTTCGGGGTTTGGCTTGATCTATGACAACTTGGAGCATGCAAAGAAATACGAACCCAAGTACAGGCTTATCAGGAATGGA
CTCGCAACCAAGGTAGAGAAATCCAGGAAGCAAATGAAGGAAAGGAAGAACAGAGCTAAGAAAATTCGTGGAATCAAGAAGACTAAGGCTGGAGATGCAGCTAAGAAGAAGAAATGATGGGTTTTTGCAATTAACTATAATAGGAGAGTTTTTATACATCGCTGGTTCTTAGGCATGGAATTTTGTGCAAGACTTTTTGT
TGGCTGGATTATTATCCATACAAGGTTGAGCATATCGATGACTGTATTGCAAATCACTAGAATTTATTTATTAAGCAAATTTTTGTAATGTTGCAATCTGCATTTTGTTACTTATTTAAAATTTAAAGAAAACTGTGGTATAATTGTCTTGGGAGATTTAAATGTGTTGACTTGTATTTCAATCGTCTACGTTTCTATGT
GTTTGAGATATAATTAATGCAAATTGTGGAAATATCTTGAAATTTGCGGCATTCAGACGCTTTTTGGTAAACAGAGTTTGTTGCAAAATGTTTTTCTTTAATAAATATTTTTTCTAAGTGGGTTTTATAGAGATATCTTGAAATTTTGAGCAATCGGTTTTTTGGTTTCTCTAGGTCAATTTTTTTAGTTGTAAAACGAA
GAAGACATAGCTTTTTTTAATTGGGAAGATATATTTTCCATGTAATTGTAGCTCTATTTGTAAGAGCAAGGTGACACCAAAGGAATATAAAAATGTGAGAGTTGTTTAACTTTATTTTGTTGTAGTCTCATTTTGTGCATTTAGTAAATTTTTAAGATTTATGAATCACATTTTCTAACTCACCATTTTAGGAGCCAATA
TTTGTGTTATGATCAATATTTCCAAGTTTGGGTTGGGCGTATTGAAATAACTTTTTTCGTAGGTTTTAAAGATCAGATGCAAAATAAACTAGATAGAACATTTTGATCTCATTCGGCGCTGTCTCCTTGTTACTATTATGTTCCGAAGCATAGCTTCACCGTGACTGGTTAAGGCAGATATTCAGAGTATAGATTTTAAT
TTTTTTTATCTTACCATGTTAGGTGGTAATATAACATTTTGAAATGACATCTGATACTTGACGTGGACCATCAATATCAATCAATATAATTGATTTATATTATTTATTAGTTGCTGAGATCGTGTGTATGCATTGAACCTTCCTAGCGTCAAGAGAACAGGCATGTCAAATTCTGGTCCATATAATGATTTGAACTGTAC
TTGTACTCATTGAAGCCAGAGCTATTTCCTCAAATAAACATTCCAGAATCCATGGTAATTGATGAGATATACAAGCTATAACCAGCACTCTTCAAAGAAATATTGAAACATTACAATATCTGATGGGATCATAAGCAATAACCATC

>TRINITY_DN110672_c4_g6_i5 from sample A5 / Example unspliced isoform:

AGGGTTTCGGCAAAGCATAGGAAGACAGTGTTCTCTGAGCTCACACCGAGGAGAAGAGCCGAGGCCGCGAGATCCGTGAGGAGGAAGGAGCCGAATACCATCAACAATGGCGGATGCTAAAGCCGTTACAGTCAGAACTCGCAAGTTCATGACTAACAGGCTTCTATCAAGGAAGCAATTCgtaagtatttgagtgcctt
ccaacaaaactgttgttttgagtactgctttcggtccattgggtgtctgaatattaatttacttcgctattttctcttcaatttcacttgtcacagGTGATTGATGTTCTTCATCCTGGTAGGCCAAATGTATCCAAGGTAAGCAGATCGATTATAATCATATTATGGGTCTTGTGATTAATGGATTTCATGCATTTGCG
ACACTTTGGGTTTGGAGATTTGTTTGTTGGGTAGATGTTTATAGTATAGTTTTGAGCCCAATGCAGTCGTTGGCTGTTTGTATATTGAATATTGGTTCCATTTTCGTTGAAGGCCGAGCTGAAGGAGAAGCTGGCAAAGCTGTACGAGGTCAGAGATCCGCAGACAATATTTGTTTTTGGGTTCAGAACCCAATTCGGAG
GTGGAAAATCTTCGGGGTTTGGCTTGATCTATGACAACTTGGAGCATGCAAAGAAATACGAACCCAAGTACAGGCTTATCAGGGTAAGTCCCATGCATGTTTTCTGTTATTTAATAGTGGGCAGTCATTCGGGCTTCTCTTGTTTTATGGTGTTACCCTCATTCGGATGCCGTGGATAAGAAATTTTCATAAGATTTTCA
AGGTTAAAGTAGGTCATTCTTTTAGACGGGAAAAAATGAACGATACCCAATAATGGCTGATGTGGGATGTTTATTTTAATCCCTGCCATGTATCGATCTTAGATATCTAATCATAGACCCACACGGAATCTCAAATCTGATAATTTCAATTTATACCCTTTTGGGAAGTTTCTTGAGAAACATGATCGGAGGGGTAAGTA
TTCTGTAAAAAACGGCCTGAAACTTCCACCCGGTCTTCTCTCTTAGAACTTATTCAATATGGGTGGGGCAGGCCGAAGCGAGAACAGGACAGTGGTGGACAGCTTAATACACCACCAACCTCACCTTTAAAGAACGTACTATGGCAAAATTTTGTGCCATTTACTGAATTCAACATTGCCTCGTTTTCCATCATTTTCAT
GTACAGTATTCTTATCTATAAATGACATCAGCCCATGGTATGCTTTTAAAGAATTGAGGTTCGGAACCCTAATCCTCACAAGCACTTCTGCTAGGATATTTTCAGAGAACTTTGTAACCAGTGTCCTTATCTTCTTCAGTGTCAGTGGAACGAGTGTTGTCAAGAGAAGAGTAGAACTACGTTTTTGTATAATTTCTTTC
TGAATATTTTTTATATCCCAATTTGCAATTATGCTTTTCTACCACTTGTATATTTAAACTTCCAATCAGTGTTGTATAATAATATAATAAACATGTGATATATCATTTATGTCAAGTGATATATCATTTATGTCATTAATCAATGATACATATCCCATGAGTATAGTAAAGTTAGTCATTAACCCATATCCTATGAGTAT
AGTCGCAGCTGACCAAGATGATCCTACAGTAAGGATCGAAACGTCGAGAACTAGCATTTCACGAACATAGATCCTCTCTTCTCTCAAATCCATCTCTGCGTATGGCACTTTATAAACAATTTCTCATGGTTTTGAATAATCCACTTAGCAATCATTGCATTGGGTGATGCTCACAGGGGTGAAGAAGTGAGATTGAAAC

Intron 3 GTAAGCAGAT ... TTCGTTGAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	10.24	570	1	65
A2	12.46	569	5.5	81
A3	13.03	734	2.5	110
A4	12.3	335	3.5	47
A5	15.95	261	6.5	49.5
A6	13.69	1979	0	314
A7	12.72	3946	1	575
A8	15.54	3721.8	0	685
A9	13.31	4167	0.5	640
A10	10.87	3492	1	426

>TRINITY_DN100378_c3_g4_i5 from sample A4 / Example spliced isoform

AGGGTTTCGGCAAAGCATAGGAAGACAGTGTTCTCTGAGCTCACACCGAGGAGAAGAGCCGAGGCCGCGAGATCCGTGAGGAGGAAGGAGCCGAATACCATCAACAATGGCGGATGCTAAAGCCGTTACAGTCAGAACTCGCAAGTTCATGACTAACAGGCTTCTATCAAGGAAGCAATTCGTGATTGATGTTCTTCATC
CTGGTAGGCCAAATGTATCCAAGGCCGAGCTGAAGGAGAAGCTGGCAAAGCTGTACGAGGTCAGAGATCCGCAGACAATATTTGTTTTTGGGTTCAGAACCCAATTCGGAGGTGGAAAATCTTCGGGGTTTGGCTTGATCTATGACAACTTGGAGCATGCAAAGAAATACGAACCCAAGTACAGGCTTATCAGGAATGGA
CTCGCAACCAAGGTAGAGAAATCCAGGAAGCAAATGAAGGAAAGGAAGAACAGAGCTAAGAAAATTCGTGGAATCAAGAAGACTAAGGCTGGAGATGCAGCTAAGAAGAAGAAATGATGGGTTTTTGCAATTAACTATAATAGGAGAGTTTTTATACATCGCTGGTTCTTAGGCATGGAATTTTGTGCAAGACTTTTTGT
TGGCTGGATTATTATCCATACAAGGTTGAGCATATCGATGACTGTATTGCAAATCACTAGAATTTATTTATTAAGCAAATTTTTGTAATGTTGCAATCTGCATTTTGTTACTTATTTAAAATTTAAAGAAAACTGTGGTATAATTGTCTTGGGAGATTTAAATGTGTTGACTTGTATTTCAATCGTCTACGTTTCTATGT
GTTTGAGATATAATTAATGCAAATTGTGGAAATATCTTGAAATTTGCGGCATTCAGATGCTTTTTGGTAAACAGAGTTTGTTGCAAAATGTTTTTCTTTAATAAATATTTTTTCTAAGTGGGTTTTATAGAGATATCTTGAAATTTTGAG

>TRINITY_DN100378_c3_g4_i1 from sample A4 / Example unspliced isoform:

TGGTAGGCCAAATGTATCCAAGgtaagcagatcgattataatcatattatgggtcttgtgattaatggatttcatgcatttgcgacactttgggtttggagatttgtttgttgggtagatgtttatagtatagttttgagcccaatgcagtcgttggctgtttgtatattgaatattggttccattttcgttgaagGCCG
AGCTGAAGGAGAAGCTGGCAAAGCTGTACGAGGTCAGAGATCCGCAGACAATATTTGTTTTTGGGTTCAGAACCCAATTCGGAGGTGGAAAATCTTCGGGGTTTGGCTTGATCTATGACAACTTGGAGCATGCAAAGAAATACGAACCCAAGTACAGGCTTATCAGGGTAAGTCCCATGCATGTTTTCTGTTATTTAATA
GTGGGCAGTCATTCGGGCTTCTCTTGTTTTATGGTGTTACCCTCATTCGGATGCCGTGGATAAGAAATTTTCATAAGATTTTCAAGGTTAAAGTAGGTCATTCTTTTAGACGGGAAAAAATGAACGATACCCAATAATGGCTGATGTGGGATGTTTATTTTAATCCCTGCCATGTATCGATCTTAGATATCTAATCATAC
ACCCACACGGAATCTCAAATCTGATAATTTCAATTTATACCCTTTTGGGAAGTTTCTTGAGAAACATGATCGGAGGGGTAAGTATTCTGTAAAAAACGGCCTGAAACTTCCACCCGGTCTTCTCTCTTAGAACTTATTCAATATGGGTGGGGCAGGCCGAAGCGAGAACAGGACAGTGGTGGACAGCTTAATACACCACC
AACCTCACCTTTAAAGAACGTACTATGGCAAAATTTTGTGCCATTTACTGAATTCAACATTGCCTCGTTTTCCATCATTTTCATGTACAGTATTCTTATCTATAAATGACATCAGCCCATGGTATGCTTTTAAAGAATTGAGGTTCGGAACCCTAATCCTCACAAGCACTTCTGCTAGGATATTTTCAGAGAACTTTGTA
ACCAGTGTCCTTATCTTCTTCAGTGTCAGTGGAACGAGTGTTGTCAAGAGAAGAGTAGAACTACGTTTTTGTATAATTTCTTTCTGAATATTTTTTATATCCCAATTTGCAATTATGCTTTTCTACCACTTGTATATTTAAACTTCCAATCAGTGTTGTATAATAATATAATAAACATGTGATATATCATTTATGTCAAG
TGATATATCATTT