sp|P49310|GRP1_SINAL ----> Glycine-rich RNA-binding protein GRP1A

Intron 1 GTCTAGGCTG ... GCAATTATAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	32.14	133	6	63
A2	36.71	100	5	58
A3	37.93	144	5	88
A4	37.01	97	6	57
A5	33.9	78	5	40
A6	22.22	7	1	2
A7	35.1	98	2	53
A8	23.08	10	0	3
A9	30.7	79	2	35
A10	36.8	79	3	46

>TRINITY_DN48236_c1_g6_i3 from sample A10 / Example spliced isoform

TGACGAGTGGCTCAACTGACCAAGATTTGGAACTGAATTCCAAACCTGTAAAGAATCTGAATCTTTTTGCGTTGAAGTTACACGAAAACAACGACTCCGTACATTCAACGCTCTTATTATAACCGTTGTGCACAAATCCCTGTTGTGGAGTACCGGGCACCAACTCTTCCACTTTCCGGACACATTTCTTCTTGTTTTCG

CTCCAGGCAATTTACAAGAATTTTGGACAATAATGGAACCTGGAGAGGAGCAATTAGATATTGAGGATGATGATTTTGGAGGAGTTGAGCAGAACAGCATTTTTCAGACAGGTCAGACAGTTGATGCAACATGGGTTGATGATGATGAAGGTGATGATCTTTACAATGATTTAAACGTGAGTTTTATCCAGGCACCTGTG
ATAGCCTACCACAGCACCAAGCAGGAACATGTTGGGAATGGAAGGGGTCAGCAAAAACTTCCATATGAAATTGAGCAGACTAAGGGACCAATTCCAGGGGAAGGTAGAATTGGTGAGCCTTTTGCTGGTAGTGTGGGGTGCATCAAACATGATGAAATTAATCAAGTTGAACAAGGAGGGATCAGTGGGAATATTACTGA
ATTTTCTATTCAGTCTCGTCCTAAAAAGGACCATTGGAATGATGATGAGGTAATGCAGCACAGAAGAGGAATGAAAAAGGAGCTGAGATTTGGGCATCCTGTGCCTATTTCTTCAACGACTTCATTTACATCGAATGGTAATAAGGCTCCAAAAAATCATACAATGTATGAGGATGATGAATCATATATGCAGCAGGTTT
CCCAAGGCACAAATCAATATGATAAAACTGACGAAGGACATAAGCTTGTTGTTGTACCCGAACAAAGGCAGTCATTTCCTGGATCCAGACTTGTTGCTTCTCCATTGGAAGATATGGCTAGTCAAGTAAGCGCAGAAGCAGTAGAATCAAATATGGAAGTACCTGAAGTGTTCCAAGGTCATGCTAGAAATGCAACTGCA
AGCTTTAGAGAGTCTGCAGCATCAGGCAAAGCAGGCAGATTGAATGTTGGTGATGGATCAATAAATTTGGGAGGAAACAATTTTGGTGCTTCTTCTGGACAGGACAGTACATCATCTCCTGCTGTCTCCCCTTCCAACAGAATAGGTCCAGCCTCGCCTCCTGCTGCCTCCACTTCCAACAGAAAAGGTCGAGTTCCAAC
TGAGTGCGTAGGTCAACAAGATAATTCATTTTTGCATTCAGGTGTAGAACGAGGAACAATGCTGTTTGTGGGAGAGCTGCACTGGTGGAC

>TRINITY_DN48236_c1_g6_i1 from sample A10 / Example unspliced isoform:

TGACGAGTGGCTCAACTGACCAAGATTTGGAACTGAATTCCAAACCTGTAAAGAATCTGAATCTTTTTGCGTTGAAGTTACACGAAAACAACGACTCCGTACATTCAACGCTCTTATTATAACCGTTGTGCACAAATCCCTGTTGTGGAGTACCGGGCACCAACTCTTCCACTTTCCGGACACATTTCTTCTTGTTTTCG

CTCCAGgtctaggctgttagggcatgtacttatagttacatctcttcttgtgcaattatagGCAATTTACAAGAATTTTGGACAATAATGGAACCTGGAGAGGAGCAATTAGATATTGAGGATGATGATTTTGGAGGAGTTGAGCAGAACAGCATTTTTCAGACAGGTCAGACAGTTGATGCAACATGGGTTGATGATGA
TGAAGGTGATGATCTTTACAATGATTTAAACGTGAGTTTTATCCAGGCACCTGTGATAGCCTACCACAGCACCAAGCAGGAACATGTTGGGAATGGAAGGGGTCAGCAAAAACTTCCATATGAAATTGAGCAGACTAAGGGACCAATTCCAGGGGAAGGTAGAATTGGTGAGCCTTTTGCTGGTAGTGTGGGGTGCATCA
AACATGATGAAATTAATCAAGTTGAACAAGGAGGGATCAGTGGGAATATTACTGAATTTTCTATTCAGTCTCGTCCTAAAAAGGACCATTGGAATGATGATGAGGTAATGCAGCACAGAAGAGGAATGAAAAAGGAGCTGAGATTTGGGCATCCTGTGCCTATTTCTTCAACGACTTCATTTACATCGAATGGTAATAAG
GCTCCAAAAAATCATACAATGTATGAGGATGATGAATCATATATGCAGCAGGTTTCCCAAGGCACAAATCAATATGATAAAACTGACGAAGGACATAAGCTTGTTGTTGTACCCGAACAAAGGCAGTCATTTCCTGGATCCAGACTTGTTGCTTCTCCATTGGAAGATATGGCTAGTCAAGTAAGCGCAGAAGCAGTAGA
ATCAAATATGGAAGTACCTGAAGTGTTCCAAGGTCATGCTAGAAATGCAACTGCAAGCTTTAGAGAGTCTGCAGCATCAGGCAAAGCAGGCAGATTGAATGTTGGTGATGGATCAATAAATTTGGGAGGAAACAATTTTGGTGCTTCTTCTGGACAGGACAGTACATCATCTCCTGCTGTCTCCCCTTCCAACAGAATAG
GTCCAGCCTCGCCTCCTGCTGCCTCCACTTCCAACAGAAAAGGTCGAGTTCCAACTGAGTGCGTAGGTCAACAAGATAATTCATTTTTGCATTCAGGTGTAGAACGAGGAACAATGCTGTTTGTGGGAGAGCTGCACTGGTGGAC

Intron 2 GTCAGACAGT ... TTTTATCCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	88.03	24	145	208
A2	91.03	17	155	190
A3	85.99	29	140	216
A4	88.8	14	92	130
A5	87.6	8	44	69
A6	93.75	1	18	12
A7	91.75	12	118	149
A8	83.56	6	23	38
A9	88.24	14	88	122
A10	86.8	20	99	164

>TRINITY_DN48236_c1_g6_i2 from sample A10 / Example spliced isoform

CAGAACAGCATTTTTCAGACAGGAACCTGGGATTGCCTACCACAGCACCAAGCAGGAACATGTTGGGAATGGAAGGTGCCAGCAAAAACTTCAATATGAAATTGAGCAGACTAAGGGACCAATTCCAGGGGAAGGTAGAATTGGTGAGCCTTTTGCTGGTAGTGTGGGGTGCATCAAACATGATGAAATTAATAAAGTTG
AGCAAGGAGGGATCAGTGGGAATATTACTGAATTTTCTATTCAGTCTCGTCCTAAAAAGGACCATTGGAATGATGATGAGGTAAAACAGCACAGAGGAGGAATGAAAAAGGAACTGAGATTTGGGCATCCTGGGCCTACTTCTTCAACGACTTCATTTACATCGAATGGTAACAAGGCTCCAAAAAATCGTACAATGTAT
AAGGATGATGAATCATATATGCAGCAGGTTTCCCAAGGCACAAATCAATATGATAAAACTGACGAAGGACATAAGCTTGTTGTTGTACCCGAACAAAGGCAGTCATTTCCTGGATCCAGACTTGTTGCTTCTCCATTGGAAGATAGGGGTAGTCAAGTAAGCGCAGAAGCAG

>TRINITY_DN48236_c1_g6_i4 from sample A10 / Example unspliced isoform:

GATTTACAAGAATTTTGGGTAATAATGGAACCTGGAGAGGAGCAATTAGATATTGAGGATGATGATTTTGGAGGAGTTGAGCAGAACAGCATTTTTCAGACAGgtcagacagttgatgcaacctgggctgatgatgatgaatgtgatgatctttacaatgatttaaacgtgagttttatccagGAACCTGGGATTGCCTA
CCACAGCACCAAGCAGGAACATGTTGGGAATGGAAGGTGCCAGCAAAAACTTCAATATGAAATTGAGCAGACTAAGGGACCAATTCCAGGGGAAGGTAGAATTGGTGAGCCTTTTGCTGGTAGTGTGGGGTGCATCAAACATGATGAAATTAATAAAGTTGAGCAAGGAGGGATCAGTGGGAATATTACTGAATTTTCTA
TTCAGTCTCGTCCTAAAAAGGACCATTGGAATGATGATGAGGTAAAACAGCACAGAGGAGGAATGAAAAAGGAACTGAGATTTGGGCATCCTGGGCCTACTTCTTCAACGACTTCATTTACATCGAATGGTAACAAGGCTCCAAAAAATCGTACAATGTATAAGGATGATGAATCATATATGCAGCAGGTTTCCCAAGGC
ACAAATCAATATGATAAAACTGACGAAGGACATAAGCTTGTTGTTGTACCCGAACAAAGGCAGTCATTTCCTGGATCCAGACTTGTTGCTTCTCCATTGGAAGATATGGCTAGTCAAGTAAGCGCAGAAGCAGTAGAATCAAATATGGAAGTACCTGAAGTGTTCCAAGGTCATGCTAGAAATGCAACTGCAAGCTTTAG
AGAGTCTGCAGCATCAGGCAAAGCAGGCAGATTGAATGTTGGTGATGGATCAATAAATTTGGGAGGAAACAATTTTGGTGCTTCTTCTGGACAGGACAGTACATCATCTCCTGCTGTCTCCCCTTCCAACAGAATAGGTCCAGCCTCGCCTCCTGCTGCCTCCACTTCCAACAGAAAAGGTCGAGTTCCAACTGAGTGCG
TAGGTCAACAAGATAATTCATTTTTGCATTCAGGTGTAGAACGAGGAACAATGCTGTTTGTGGGAGAGCTGCACTGGTGGAC