NA ----> NA

Intron 1 GTACATCTTA ... AAAGCCCTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	-1	0	0	0
A2	-1	0	0	0
A3	-1	0	0	0
A4	60	1	1	2
A5	-1	0	0	0
A6	100	0	1	1
A7	77.27	5	16	18
A8	66.67	1	2	2
A9	57.14	3	4	4
A10	84.62	1	7	4

>TRINITY_DN33193_c0_g1_i1 from sample A9 / Example spliced isoform

CTCTGCCATTATCCTTCCCGGTCTTTACTACTGAAGGGTTTTCATTGGAAGGAGATCTAGAGGTGTAGGGTTTTCGCTGTTTTTGCAACTTCGCTGGCTTACGTTTGATTGTTTCAAGCATTCAGAATCCATGGCTTACACGGACAACACCGAACGCGTATCTCCAGAGGAGGAAGGCTGAGCTAGCAGATTGTTTCCGC
CAAATTGAAGCCCTAAAGGTCGTCATGTATGGAGAATATAAATTCTGTCGTGAACGAGGCGAAAGTGAATTTAGCGGAGAAAGCGCGGGCGCTACAGAGGGTTATGGATGAATTATGCCTGCAGGGAGCGAATAATAGTTTTTCCGTAGGTTCTAGCCATTGAATTCACAGTGAGTGTGGACCCAACAACCTTCCTTCGA
AAATGGCGATCTATGTGTCTAATTCTTCTTCAGAAACTACTAACATAATTATAGAGTAGATGGATAGAGAAGCAGCGGCTTTATTCCAAAGACTGTGAGCAATGGCTATGTGACATACCAAGAAATACTGCAAGAAGTACAGCACGCTGATCATGAAGAGAAGATTGTGATGGCAGCTGCAGGTCGCATGAATGAATCCG
CAGGAGAACCTGAAATCTTCGAGATAGAAACGGGTCAAGAGGAAACACCTGCTTCTAGCAAGAAAAGTGGGTTCCTTAAAAACAAATTACATCGCCGCGGTGATGATAATTCACAAAGCTCAGAAAATAGAAAGAGAGGCACATTTCGTATGTTCTTTAGAAAGTTTTCAACATGCCTCCATGCGGAAGGATAAATCTAG
CAATGTAGACGGGCGGTGATCGAATTGCTTTGAATCAGTGGAAGGCGAGCTTCTTTCAGTTTGTTTTTATGCTTTCAAAGTGCATTAGGGTTTTTAAAAAAACTTGTGTTGACTGAACATTTAAACATGGTGAAGGTTAATGTCCATGTTGGCTAACATAGGTTTGAACGGTTTGACCATTAACAATTTTGAGGTTTTAA
ATGTACCAAGAAAGTGGTTTTATTATACTGGAT

>TRINITY_DN33193_c0_g1_i2 from sample A9 / Example unspliced isoform:

CTCTGCCATTATCCTTCCCGGTCTTTACTACTGAAGGGTTTTCATTGGAAGGAGATCTAGAGGTGTAGGGTTTTCGCTGTTTTTGCAACTTCGCTGGCTTACGTTTGATTGTTTCAAGCATTCAGAATCCATGGCTTACACGGACAACACCGAACGCGTATCTCCAGAGGAGGAAGGCTGAGCTAGCAGATTGTTTCCGC
CAAATTGAAGCCCTAAAGgtacatcttattgtttgttttacatgttttcagtaggcaaaaattccatgtatatttcttcaattaaagccctagGTCGTCATGTATGGAGAATATAAATTCTGTCGTGAACGAGGCGAAAGTGAATTTAGCGGAGAAAGCGCGGGCGCTACAGAGGGTTATGGATGAATTATGCCTGCAGG
GAGCGAATAATAGTTTTTCCGTAGGTTCTAGCCATTGAATTCACAGTGAGTGTGGACCCAACAACCTTCCTTCGAAAATGGCGATCTATGTGTCTAATTCTTCTTCAGAAACTACTAACATAATTATAGAGTAGATGGATAGAGAAGCAGCGGCTTTATTCCAAAGACTGTGAGCAATGGCTATGTGACATACCAAGAAA
TACTGCAAGAAGTACAGCACGCTGATCATGAAGAGAAGATTGTGATGGCAGCTGCAGGTCGCATGAATGAATCCGCAGGAGAACCTGAAATCTTCGAGATAGAAACGGGTCAAGAGGAAACACCTGCTTCTAGCAAGAAAAGTGGGTTCCTTAAAAACAAATTACATCGCCGCGGTGATGATAATTCACAAAGCTCAGAA
AATAGAAAGAGAGGCACATTTCGTATGTTCTTTAGAAAGTTTTCAACATGCCTCCATGCGGAAGGATAAATCTAGCAATGTAGACGGGCGGTGATCGAATTGCTTTGAATCAGTGGAAGGCGAGCTTCTTTCAGTTTGTTTTTATGCTTTCAAAGTGCATTAGGGTTTTTAAAAAAACTTGTGTTGACTGAACATTTAAA
CATGGTGAAGGTTAATGTCCATGTTGGCTAACATAGGTTTGAACGGTTTGACCATTAACAATTTTGAGGTTTTAAATGTACCAAGAAAGTGGTTTTATTATACTGGAT

Intron 2 GTACATCTTA ... TGAAGAGAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	-1	0	0	0
A2	100	0	0	2
A3	100	0	0	1
A4	100	0	1	0
A5	-1	0	0	0
A6	100	0	1	2
A7	80.95	2	16	1
A8	100	0	2	3
A9	63.64	2	4	3
A10	65.22	4	7	8

>TRINITY_DN34688_c0_g1_i2 from sample A10 / Example spliced isoform

ATCTAGAGGTGTAGGGTTTTCGCTGTTTTTGCAACTTCGCTGGCTTACGTTTGATTGTTTCAAGCATTCAGAATCCATGGCTTACACGGACAACACCGAACGCGTATCTCCAGAGGAGGAAGGCTGAGCTAGCAGATTGTTTCCGCCAAATTGAAGCCCTAAAGATTGTGATGGCAGCTGCAGGTCGCATGAATGAATCC
GCAGGAGAACCTGAAATCTTCGAGATAGAAACGGGTCAAGAGGAAACACCTGCTTCTAGCAAGAAAAGTGGGTTCCTTAAAAACAAATTACATCGCCGCGGTGATGATAATTCACAAAGCTCAGAAAATAGAAAGAGAGGCACATTTCGTATGTTCTTTAGAAAGTTTTCAACATGCCTCCATGCGGAAGGATAAATCTA
GCAATGTAGACGGGCGGTGATCGAATTGCTTTGAATCAGTGGAAGGCGAGCTTCTTTCAGTTTGTTTTTATGCTTTCAAAGTGCATTAGGGTTTTTAAAAAAACTTGTGTTGACTGAACATTTAAACATGGTGAAGGTTAATGTCCATGTTGGCTAACATAGGTTTGAACGGTTTGACCAAAAAAA

>TRINITY_DN34688_c0_g1_i1 from sample A10 / Example unspliced isoform:

ATCTAGAGGTGTAGGGTTTTCGCTGTTTTTGCAACTTCGCTGGCTTACGTTTGATTGTTTCAAGCATTCAGAATCCATGGCTTACACGGACAACACCGAACGCGTATCTCCAGAGGAGGAAGGCTGAGCTAGCAGATTGTTTCCGCCAAATTGAAGCCCTAAAGgtacatcttattgtttgttttacatgttttcagtag
gcaaaaattccatgtatatttcttcaattaaagccctaggtcgtcatgtatggagaatataaattctgtcgtgaacgaggcgaaagtgaatttagcggagaaagcgcgggcgctacagagggttatggatgaattatgcctgcagggagcgaataatagtttttccgtaggttctagccattgaattcacagtgagtgtg
gacccaacaaccttccttcgaaaatggcgatctatgtgtctaattcttcttcagaaactactaacataattatagagtagatggatagagaagcagcggctttattccaaagactgtgagcaatggctatgtgacataccaagaaatactgcaagaagtacagcacgctgatcatgaagagaagATTGTGATGGCAGCTG
CAGGTCGCATGAATGAATCCGCAGGAGAACCTGAAATCTTCGAGATAGAAACGGGTCAAGAGGAAACACCTGCTTCTAGCAAGAAAAGTGGGTTCCTTAAAAACAAATTACATCGCCGCGGTGATGATAATTCACAAAGCTCAGAAAATAGAAAGAGAGGCACATTTCGTATGTTCTTTAGAAAGTTTTCAACATGCCTC
CATGCGGAAGGATAAATCTAGCAATGTAGACGGGCGGTGATCGAATTGCTTTGAATCAGTGGAAGGCGAGCTTCTTTCAGTTTGTTTTTATGCTTTCAAAGTGCATTAGGGTTTTTAAAAAAACTTGTGTTGACTGAACATTTAAACATGGTGAAGGTTAATGTCCATGTTGGCTAACATAGGTTTGAACGGTTTGACCA
AAAAAA