sp|Q42396|CDPKC_ARATH ----> Calcium-dependent protein kinase 12

Intron 1 GTATTTTTTC ... TTCAAGGAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	74.1	65	32	186
A2	84.86	33	18	185
A3	85.77	35	41	211
A4	84.77	37	37	206
A5	83.85	21	26	109
A6	81.34	167	101	728
A7	82.6	305	263	1448
A8	81.81	181	132	814
A9	78.42	505	373	1835
A10	81.43	378	341	1658

>TRINITY_DN135660_c3_g1_i5 from sample A1 / Example spliced isoform

CAACTTCACGTATCTCTGTTCTTTATTCTTGGGGGCAAGCAGTAAAATATTCATCGTGCAATCGCAATCTCAAAGTGATCAACTTAACTTGAAACCATGCTGCGCGCGATACAATTTGTGTGCCCTTGTTGCAGCATATGTGAGGACGATTCCAACCTTTTAGTGGACAAGGTCGATCATGAGATTGGGGAGCTTTTGGT
GGAGAAGAGATATGAGATTG

>TRINITY_DN135660_c3_g1_i2 from sample A1 / Example unspliced isoform:

CAACTTCACGTATCTCTGTTCTTTATTCTTGGGGGCAAGCAGTAAAATATTCATCGTGCAATCGCAATCTCAAAGTGATCAACTTAACgtattttttcagaggatcatatttgttctttatctattctttggttcgtgattagctgttaaagagctgctctgttctgttctgttcttttatctggactgttaaatttgta
ttgtcatttctttcaaggaagTTGAAACCATGCTGCGCGCGATACAATTTGTGTGCCCTTGTTGCAGCATATGTGAGGACGATTCCAACCTTTTAGTGGACAAGGTCGATCATGAGATTGGGGAGCTTTTGGTGGAGAAGAGATATGAGATTGGGGAGGTGATTGGCCAAGGACGGTTGGGAGTAGTGCACAAGTGCATT
CACAGGAGAAACAGACAAGAATTCGCCTGTAAAAAGATGCCTATTACCAAGAGTGCATATAATGAGATCGCTATACTCGAAAACCACTTATACAATGGACCCCAAGGAATCTTGCGTAT

Intron 2 GTATTTTTTT ... TTCAAGGAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	65.06	94	29	175
A2	67.21	81	14	166
A3	74.9	64	41	191
A4	70.48	80	33	191
A5	73.48	35	22	97
A6	70.84	282	95	685
A7	73.61	492	237	1372
A8	72.75	294	126	785
A9	69.46	765	355	1740
A10	72.76	591	290	1579

>TRINITY_DN46931_c0_g1_i9 from sample A10 / Example spliced isoform

TGGTGTGGGAGTTTACGGTACTGAAGTGCTGCGAGTTTACGGTACTTAAGTTTTGAGAGCATCATATTTGTTCTTGACTTAGAGGATCAACTTCACGGATCTCTGTTCTTTATTCTTGGGGGCAAGCAGTAAAATATTCATCGTGCAATCGCAATCTCAAAGTGATCAACTTAACTTGAAACCATGCTGCGCGCGATACA
ATTTGTGTGCCCTTGTTGCAGCATATGTGAGGACGATTCCAACCTTTTAGTGGACAAGGTCGATCATGAGATTGGGGAGCTTTTGGTGGAGAAGAGATATGAGATTGGGGAGGTGATTGGCCAAGGACGGTTGGGAGTAGTGCACAAGTGCATTCACAGGAGAACCAGACAAGAATTCGCCTGTAAAAAGATGCCTATTA
CCAAGAATGCATATAATGAGATCACTATACTTGAAAACCACTTATACAACGGACCCCAAGGAATCTTGCGTATCAGAGACCACTGCTGGCTGGAGCAGGATAGATTGTCAAAGAATGTCTACATGATTTC

>TRINITY_DN46931_c0_g1_i10 from sample A10 / Example unspliced isoform:

TGGTGTGGGAGTTTACGGTACTGAAGTGCTGCGAGTTTACGGTACTTAAGTTTTGAGAGCATCATATTTGTTCTTGACTTAGAGGATCAACTTCACGGATCTCTGTTCTTTATTCTTGGGGGCAAGCAGTAAAATATTCATCGTGCAATCGCAATCTCAAAGTGATCAACTTAACgtatttttttgagaggatcacattt
gttctttatctattctttggttcgtgattagctgttaaagagcttctctgttctgttctgttcttttatctggactgttaaatttgtattgtcatttctttcaaggaagTTGAAACCATGCTGCGCGCGATACAATTTGTGTGCCCTTGTTGCAGCATATGTGAGGACGATTCCAACCTTTTAGTGGACAAGGTCGATCA
TGAGATTGGGGAGCTTTTGGTGGAGAAGAGATATGAGATTGGGGAGGTGATTGGCCAAGGACGGTTGGGAGTAGTGCACAAGTGCATTCACAGGAGAACCAGACAAGAATTCGCCTGTAAAAAGATGCCTATTACCAAGAATGCATATAATGAGATCACTATACTTGAAAACCACTTATACAACGGACCCCAAGGAATCT
TGCGTATCAGAGACCACTGCTGGCTGGAGCAGGATAGATTGTCAAAGAATGTCTACATGATTTC

Intron 3 GTGATCAACT ... TCGTTTCAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	98.82	1	132	36
A2	96.51	3	137	29
A3	98.06	2	164	38
A4	97.42	3	181	46
A5	100	0	57	40
A6	97.97	6	443	135
A7	98.73	9	1047	348
A8	97.61	9	532	202
A9	96.81	26	1193	386
A10	97.23	24	1195	490

>TRINITY_DN46931_c0_g1_i17 from sample A10 / Example spliced isoform

GGCACGCTCTTGTATGATTTGAGTTAACGATCCGGGCGGGGCTAACGGTTTCGCTAAAATGCTAGTGGATACGCTATGGTGTGGGACTTTACGGTACTTCACCGCTGCTTCTTCCAGTACAACTGAGCCGCTAATATATCATACGTTCTTCGTGTTGCCTGCCTGCACATTGGCTTGGGCTCTGCATTAAAAGCACTTCG
GATCAGGAGAGGAGGCCAACCAGTAAATATTCATATTGCAATCTCAGAAGAAGTTGAAACCATGCTGCGCGCGATACAATTTGTGTGCCCTTGTTGCAGCATATGTGA

>TRINITY_DN46931_c0_g1_i13 from sample A10 / Example unspliced isoform:

GGCACGCTCTTGTATGATTTGAGTTAACGATCCGGGCGGGGCTAACGGTTTCGCTAAAATGCTAGTGGATACGCTATGGTGTGGGACTTTACGGTACTTCACCGCTGCTTCTTCCAGTACAACTGAGCCGCTAATATATCATACGTTCTTCGTGTTGCCTGCCTGCACATTGGCTTGGGCTCTGCATTAAAAGCACTTCG
GATCAGGAGAGGAGGCCAACCAGTAAATATTCATATTGCAATCTCAGAAgtgatcaacttaacgtattttttgagaggatcatatttgttcttttatctattctttggttcctgattacctgttaaagagcttctctgttctgttctttattcttgactgttaaatttgtattgtccatcgtttcaagGAAGTTGAAACC
ATGCTGCGCGCGATACAA

Intron 4 GTGATCAACT ... TTCAAGGAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	68.13	87	25	186
A2	69.55	81	14	185
A3	76.17	66	37	211
A4	72.54	78	34	206
A5	76.22	34	20	109
A6	72.22	280	82	728
A7	74.99	483	211	1448
A8	74.27	282	105	814
A9	70.58	765	309	1835
A10	73.66	593	280	1658

>TRINITY_DN135660_c3_g1_i3 from sample A1 / Example spliced isoform

TTTGAGTTAACGATCCGGGCGGGGCTAACGGTTTCGCTAAAATGCTAGTGGATACGCTATGGTGTGGGAGTTTACGGTACTGAAGTGCTGCGAGTTTACGGTACTTAAGTTTTGAGAGCATCATATTTGTTCTTGACTTAGAGGATCAACTTCACGTATCTCTGTTCTTGGGGGCAAGCAGTAAAATATTCATCGTGCAA
TCGCAATCTCAAATTGAAACCATGCTGCGCGCGATACAATTTGTGTGCCCTTGTTGCAGCATATGTGAGGACGATTCCAACCTTTTAGTGGACAAGGTCGATCATGAGATTGGGGAGCTTTTGGTGGAGAAGAGATATGAGATTGAGGAGGTGATTGGGCAAGGACGGTTGGGAGTAGTGCACAAGTGCATTCACAGGAG
AAACAGACAAGAATTCGCCTGTAAAAAGATGCC

>TRINITY_DN135660_c3_g1_i2 from sample A1 / Example unspliced isoform:

CAACTTCACGTATCTCTGTTCTTTATTCTTGGGGGCAAGCAGTAAAATATTCATCGTGCAATCGCAATCTCAAAgtgatcaacttaacgtattttttcagaggatcatatttgttctttatctattctttggttcgtgattagctgttaaagagctgctctgttctgttctgttcttttatctggactgttaaatttgta
ttgtcatttctttcaaggaagTTGAAACCATGCTGCGCGCGATACAATTTGTGTGCCCTTGTTGCAGCATATGTGAGGACGATTCCAACCTTTTAGTGGACAAGGTCGATCATGAGATTGGGGAGCTTTTGGTGGAGAAGAGATATGAGATTGGGGAGGTGATTGGCCAAGGACGGTTGGGAGTAGTGCACAAGTGCATT
CACAGGAGAAACAGACAAGAATTCGCCTGTAAAAAGATGCCTATTACCAAGAGTGCATATAATGAGATCGCTATACTCGAAAACCACTTATACAATGGACCCCAAGGAATCTTGCGTAT