NA ----> NA

Intron 1 GTATGTGTGC ... TGTTCTGAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	77.78	4	14	14
A2	71.43	2	5	5
A3	60	1	2	1
A4	66.67	3	3	9
A5	73.22	1	4.5	1
A6	59.13	11	16	16
A7	86.66	2	11	15
A8	76.77	4	7	19.5
A9	54.45	5	4	8
A10	100	0	3	9

>TRINITY_DN44489_c5_g2_i1 from sample A8 / Example spliced isoform

GTGAATTTTGCTTTCATGATCCGTGAATTTTTCGTTAATTAAGCCATTTTTTCTCAATAAATAGGAGGTTAGGGTTAGCATACGCGAGGAAGGGTTCTGGGCGCATATTTCCGGAGGGTTAGGCCACGAAAAAGGGTTTTGGCCGCAAATTTCCAAGGGTTAGCCTGCAAGGAAGTGTGGTGGCCGCGGCTTTCCAGAAG
ATGAGCCTCCAACGAAGGGTTTTGGCCGCGGATTTTCAGAGGGTTAGAACAAGAAAAAGGGTTTTGGCCATTGTTCAGCCATTCGGGGGCTCCTGTCACGGATTTTCAAAGGTGTAGGAGTGAGCAGGAGCCATTACTTGTTCGAAAAATTGGTACTTTTGATCCTCAGTTCAATCGTTCAGTTCTATTTTTCGATGGTG
CGGCTCAAGATGGTGGCTAGTATCTTCAACATTGATGATTTGAAGATCTTTGGGGACTCAAGGGTCACAATCAAATGGGCGCAAGGGGAATATAAACTTAAAGTGTTATCACTTGTCCACTGGTGTATTCGGTTAAGATCAACTATCCTTCTTTTCAGGAATATTTCTCTGGAGCACATTTACAGGCAACACAATCAACT
GGCTGCCAAACTCTCTAACCAAGCTTTGTGCAATGATTTTGGTTTTTTAATTTGGGAAGAATTTCACTATTTCCAGGTTCTGAATTTTGGTTCTCTGAAATTTTTTGAGTAAATGCTTGTATCGGGACGATGTATGAAGTTTTTAGGATAGATGATTATGGGGTCTATGTATACCTCCTTTGATGATCAGCCTCAGTGTT
TTGAGGAGTTTTGAGATATGATGGCATGTCTTTCTGCCTTTGCTCAATGGATTCATCTTATAGACTTGTAAATCATGTACCTTTTGACTCATTTTGAATAACATCGGTATGTTTTTATGCTTTTTTCTGTTGGAAAAAAAGAAAATATATGTCAATTAGGC

>TRINITY_DN44489_c5_g2_i4 from sample A8 / Example unspliced isoform:

GTGAATTTTGCTTTCATGATCCGTGAATTTTTCGTTAATTAAGCCATTTTTTCTCAATAAATAGGAGGTTAGGGTTAGCATACGCGAGGAAGGGTTCTGGGCGCATATTTCCGGAGGGTTAGGCCACGAAAAAGGGTTTTGGCCGCAAATTTCCAAGGGTTAGCCTGCAAGGAAGTGTGGTGGCCGCGGCTTTCCAGAAG
ATGAGCCTCCAACGAAGGGTTTTGGCCGCGGATTTTCAGAGGGTTAGAACAAGAAAAAGGGTTTTGGCCATTGTTCAGCCATTCGGGGGCTCCTGTCACGGATTTTCAAAGGTGTAGGAGGAGCCATTACTTGTTCGAAAAATTGGTACTTTTGATCCTCAGTTCAATCGTTCAGTTCTATTTTTCGATGGTGCGGCTCA
AGATGgtatgtgtgctgctgggggtgctatttttttaaatcactcattattgcactttgcggctaaattgtgggaatgggacaaacatgaaagcagaacttcttgctttatggtgtgttctgaagGTGGCTAGTATCTTCAACATTGATGATTTGAAGATCTTTGGGGACTCAAGGGTCACAATCAAATGGGCGCAAGGG
GAATATAAACTTAAAGTGTTATCACTTGTCCACTGGTGTATTCGGTTAAGATCAACTATCCTTCTTTTCAGGAATATTTCTCTGGAGCACATTTACAGGCAACACAATCAACTGGCTGCCAAACTCTCTAACCAAGCTTTGTGCAATGATTTTGGTTTTTTAATTTGGGAAGAATTTCACTATTTCCAGGTTCTGAATTT
TGGTTCTCTGAAATTTTTTGAGTAAATGCTTGTATCGGGACGATGTATGAAGTTTTTAGGATAGATGATTATGGGGTCTATGTATACCTCCTTTGATGATCAGCCTCAGTGTTTTGAGGAGTTTTGAGATATGATGGCATGTCTTTCTGCCTTTGCTCAATGGATTCATCTTATAGACTTGTAAATCATGTACCTTTTGA
CTCATTTTGAATAACATCGGTATGTTTTTATGCTTTTTTCTGTTGGAAAAAAAGAAAATATATGTCAATTAGGC

Intron 2 GTAAAATTTA ... CTCATTATAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	46.03	17	14	15
A2	50	12	14	10
A3	44.19	12	10	9
A4	54.29	8	9	10
A5	31.43	12	7	4
A6	35.85	17	15	4
A7	39.13	7	8	1
A8	38.46	8	10	0
A9	45	11	12	6
A10	66.67	1	2	2

>TRINITY_DN36717_c0_g1_i5 from sample A6 / Example spliced isoform

TAGGAGGTTAGGGTTAGCATACGCGAGGAAGGGTTCTGGGCGCATATTTCCGGAGGGTTAGGCCACGAAAAAGGGTTTTGGCCGCAAATTTCCAAGGGTTAGCCTGCAAGGAAGTGTGGTGGCCGCGGCTTTCCAGAAGATGAGCCTCCAACGAAGGGTTTTGGCCGCGGATTTTCAGAGGGTTAGAACAAGAAAAAGGG
TTTTGGCCATTGTTCAGCCATTCGGGGGCTCCTGTCACGGATTTTCAAAGGTGTAGGAGGTTCTATCTCGTGTACATGTTGCTTTAATCGGGCGTCAAGGGCTTGTGTTTTGTAACGGAGTCAATAACGTAGGGATTTGGCTTTATTTTGTAGGAGGAATCATAAAATAGATTTGTGTACTCTGTATGGATTTTTTTTTT
TCCGAGGGC

>TRINITY_DN36717_c0_g1_i6 from sample A6 / Example unspliced isoform:

TAGGAGGTTAGGGTTAGCATACGCGAGGAAGGGTTCTGGGCGCATATTTCCGGAGGGTTAGGCCACGAAAAAGGGTTTTGGCCGCAAATTTCCAAGGGTTAGCCTGCAAGGAAGTGTGGTGGCCGCGGCTTTCCAGAAGATGAGCCTCCAACGAAGGGTTTTGGCCGCGGATTTTCAGAGGGTTAGAACAAGAAAAAGGG
TTTTGGCCATTGTTCAGCCATTCGGGGGCTCCTGTCACGGATTTTCAAAGGTGTAGGAGgtaaaatttacaggatcctttaacaatgtcatttaattcgtttaaagcttttccaggaaggacgtctgctgtttcttcttgttttctgttacaatgtctaatgtaagtgttttattttctctgcgaagatagttttcgagt
gcatcttttatattgggtcattttatatttttaaatgtggttaaacactcattatagGTTCTATCTCGTGTACATGTTGCTTTAATCGGGCGTCAAGGGCTTGTGTTTTGTAACGGAGTCAATAACGTAGGGATTTGGCTTTATTTTGTAGGAGGAATCATAAAATAGATTTGTGTACTCTGTATGGATTTTTTTTTTTC
CGAGGGC