sp|P92964|SRS31_ARATH ----> Serine/arginine-rich splicing factor RS31

Intron 1 GTAACTGCCA ... TTTGACGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	25.17	55	34	3
A2	21.47	64	19	16
A3	21.86	109	45	16
A4	32.93	56	36	19
A5	22.22	42	13	11
A6	22.07	83	24	23
A7	21.22	193	58	46
A8	27.2	87	29	36
A9	14.67	128	23	21
A10	15.7	196	40	33

>TRINITY_DN45976_c0_g2_i7 from sample A8 / Example spliced isoform

TCCGGCGGTTAGTGAAGAAAAGTTTGCGATTAGCAATGAAGCCATTGTACTGTGGAAACATAGAGTACGAGGTTCGACAGTCAGAGATTGAGCGCTTGTTTGGCAAATATGGAAGAGTCGAAAGGGTTGATATGAAATCAGGAATATTCAAGAGCATCCATGCTCTTCCTGGAGATCCTGTGCATATTCTTTCATCATGC
CATGGATCAGCTAATAGCCTTGTTCAGCAAAGTTGATATACAAATGACATCTTGCATTTTTCTCACGACACCATTACTTTTCAATGTTCATCCCTCCCAAGCCATTCTAAGTCTTCATTCAGGCTCAAGCATTTCAAGAGATAGAATCCATGACACATCACTGCAATCAGAATGGGGCATAACTGGTGACGGCACTTATC
TGATGGATGACCTTACAGCCACTTATGGATTAGATTTACCTGATACCATTTAGTACAACATGCAACGTTTGTGCTTTCTGTCTCCTAATGCAGCCAGTATTTACGACAATAAGTAACAGGTGTTGTATTTTTTTTTCTTTGTCGTAGCCTTATATATGAAATCATTCGCCCCAGCGCACACTGCAAATGTGATTGCAATC
CAATATTTTGTTCTTTATTGAATCTCTTGTTTCTATAGGTTATGCTTTTGTCTATATGGAAGATGAGCGTGATGCAGAGGATGCAATCCGAGCTCTTGACAAGACAGAATTTGGCAGGCAAAGGCGTCGGCTAACTGTAGAGTGGACAAAGCATGCTGAACGAGGTCTTCGCCGATCTGAAGATGGCAGAAGATCTGTTA
CAGGTTTGAAGCCAACAAAGACATTATTTGTGATAAACTTTGACCCGTACGACACTAGAGTCAGGGACCTTGAAAAACATTTTGAGCCCTATGGTAAGGTGCTCAATGTAAGGATTAGAAGGAATTTTGCATTTATTCATTACGGATCACAAGAAGAAGCAACAAAAGCTTTCAATGCCACCCATATGAGCACATTATTA
GATAGGGTAATCACAGTGGAATATGCTCAACGTGAGGATGGCAATAGAAGAGGTGGACCTTCTAGCCCCATTCGAGGAGGAGGTCGGTATGGCAGATCTGCTGAGGATCGTGGACGTGATCGGTCGGCAAGTCCTATATATGGGAGGCGTGCAATGAGGGGCAGTCCTGATTATGGTAGGGCTCCGAGTCCTATTTATTC
AAGGCGTGCAGAGAGGCGCAGTCCTGACTACGGTAGGGCTCCAAGTCCTATATATGCAAGGCGTGCAGAGAGGCGCAGTCCTGTTTATTCCAGCCGCCCCCAAAAGAATAGTCCGGATTATGGCAGAGATGCAAGCCCTATTCATGAACGGGACCGCAGTCGATCACCGGTGAGGCGGTCAGGGC

>TRINITY_DN45976_c0_g2_i1 from sample A8 / Example unspliced isoform:

TCCGGCGGTTAGTGAAGAAAAGTTTGCGATTAGCAATGAAGCCATTGTACTGTGGAAACATAGAGTACGAGGTTCGACAGTCAGAGATTGAGCGCTTGTTTGGCAAATATGGAAGAGTCGATAGGGTTGATATGAAATCAGgtaactgccatcatagtattcatattcgcacccaatatctatcgatatatctagcatgt
atttataattgtatattgctgcaacagatgtctgtaaactggtttagctgacaatattttgtcattcagatggctttggttcccaatggtataaacactgtacaaaccatccgtagtttgtcaaaccccaaacgtgtttgttctggcaggctgtttaaacctggctcaaatcgatctgaacaaataaataaatcctgttt
agtcttctagtctctgaagaatttctatctatatttagaaaactttaaatgatcttgtaagttcaatcagaaacgaatatttttaagtaggaaacctaagaggatttatcagatttgttttgtaaaatgtaaaagaccctgtgaagtctgaattgttcatggaccattgttctcaggagagttgagtgatttggttttcc
aacaatgaacgcttccatacccttacctgattaatgggtgaagccagatttgtgcttccaatggcaaactattgtgctcattggatggaaatatcaactatctaaaggaatgcagacttactgtaaaagatgcccggtggcagaagttctagctccagctattattcttaatggaatatagtatgtcaacaaaattattt
tgattttagaattggaaatcatgtcgaatgcatgattttcagtcttctttccagctggtaatgcgtaacggtaacctctatggttcatatccaaggttacaagcaaagtttaagaagcaacatttttgaaatgtaggcctctatactcaaaaatttctttgacgcagGAATATTCAAGAGCATCCATGCTCTTCCTGGAG
ATCCTGTGCATATTCTTTCATCATGCCATGGATCAGCTAATAGCCTTGTTCAGCAAAGTTGATATACAAATGACATCTTGCATTTTTCTCACGACACCATTACTTTTCAATGTTCATCCCTCCCAAGCCATTCTAAGTCTTCATTCAGGCTCAAGCATTTCAAGAGATAGAATCCATGACACATCACTGCAATCAGAATG
GGGCATAACTGGTGACGGCACTTATCTGATGGATGACCTTACAGCCACTTATGGATTAGATTTACCTGATACCATTTAGTACAACATGCAACGTTTGTGCTTTCTGTCTCCTAATGCAGCCAGTATTTACGACAATAAGTAACAGGTGTTGTATTTTTTTTTCTTTGTCGTAGCCTTATATATGAAATCATTCGCCCCAG
CGCACACTGCAAATGTGATTGCAATCCAATATTTTGTTCTTTATTGAATCTCTTGTTTCTATAGGTTATGCTTTTGTCTATATGGAAGATGAGCGTGATGCAGAGGATGCAATCCGAGCTCTTGACAAGACAGAATTTGGCAGGCAAAGGCGTCGGCTAACTGTAGAGTGGACAAAGCATGCTGAACGAGGTCTTCGCCG
ATCTGAAGATGGCAGAAGATCTGTTACAGGTTTGAAGCCAACAAAGACATTATTTGTGATAAACTTTGACCCGTACGACACTAGAGTCAGGGACCTTGAAAAACATTTTGAGCCCTATGGTAAGGTGCTCAATGTAAGGATTAGAAGGAATTTTGCATTTATTCATTACGGATCACAAGAAGAAGCAACAAAAGCTTTCA
ATGCCACCCATATGAGCACATTATTAGATAGGGTAATCACAGTGGAATATGCTCAACGTGAGGATGGCAATAGAAGAGGTGGACCTTCTAGCCCCATTCGAGGAGGAGGTCGGTATGGCAGATCTGCTGAGGATCGTGGACGTGATCGGTCGGCAAGTCCTATATATGGGAGGCGTGCAATGAGGGGCAGTCCTGATTAT
GGTAGGGCTCCGAGTCCTATTTATTCAAGGCGTGCAGAGAGGCGCAGTCCTGACTACGGTAGGGCTCCAAGTCCTATATATGCAAGGCGTGCAGAGAGGCGCAGTCCTGTTTATTCCAGCCGCCCCCAAAAGAATAGTCCGGATTATGGCAGAGATGCAAGCCCTATTCATGAACGGGACCGCAGTCGATCACCGGTGAG
GCGGTCAGGGC

Intron 2 GTGTTGTATT ... GTTTCTATAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	44.9	27	3	22
A2	38.46	32	5	20
A3	40	24	5	16
A4	53.13	15	3	17
A5	42.11	11	4	8
A6	40.76	93	3	64
A7	40	126	5	84
A8	43.07	78	12	59
A9	38.42	125	4	78
A10	40	90	14	60

>TRINITY_DN45976_c0_g2_i4 from sample A8 / Example spliced isoform

TCCGGCGGTTAGTGAAGAAAAGTTTGCGATTAGCAATGAAGCCATTGTACTGTGGAAACATAGAGTACGAGGTTCGACAGTCAGAGATTGAGCGCTTGTTTGGCAAATATGGAAGAGTCGAAAGGGTTGATATGAAATCAGGAATATTCAAGAGCATCCATGCTCTTCCTGGAGATCCTGTGCATATTCTTTCATCATGC
CATGGATCAGCTAATAGCCTTGTTCAGCAAAGTTGATATACAAATGACATCTTGCATTTTTCTCACGACACCATTACTTTTCAATGTTCATCCCTCCCAAGCCATTCTAAGTCTTCATTCAGGCTCAAGCATTTCAAGAGATAGAATCCATGACACATCACTGCAATCAGAATGGGGCATAACTGGTGACGGCACTTATC
TGATGGATGACCTTACAGCCACTTATGGATTAGATTTACCTGATACCATTTAGTACAACATGCAACGTTTGTGCTTTCTGTCTCCTAATGCAGCCAGTATTTACGACAATAAGTAACAGGTTATGCTTTTGTCTATATGGAAGATGAGCGTGATGCAGAGGATGCAATCCGAGCTCTTGACAAGACAGAATTTGGCAGGC
AAAGGCGTCGGCTAACTGTAGAGTGGACAAAGCATGCTGAACGAGGTCTTCGCCGATCTGAAGATGGCAGAAGATCTGTTACAGGTTTGAAGCCAACAAAGACATTATTTGTGATAAACTTTGACCCGTACGACACTAGAGTCAGGGACCTTGAAAAACATTTTGAGCCCTATGGTAAGGTGCTCAATGTAAGGATTAGA
AGGAATTTTGCATTTATTCATTACGGATCACAAGAAGAAGCAACAAAAGCTTTCAATGCCACCCATATGAGCACATTATTAGATAGGGTAATCACAGTGGAATATGCTCAACGTGAGGATGGCAATAGAAGAGGTGGACCTTCTAGCCCCATTCGAGGAGGAGGTCGGTATGGCAGATCTGCTGAGGATCGTGGACGTGA
TCGGTCGGCAAGTCCTATATATGGGAGGCGTGCAATGAGGGGCAGTCCTGATTATGGTAGGGCTCCGAGTCCTATTTATTCAAGGCGTGCAGAGAGGCGCAGTCCTGACTACGGTAGGGCTCCAAGTCCTATATATGCAAGGCGTGCAGAGAGGCGCAGTCCTGTTTATTCCAGCCGCCCCCAAAAGAATAGTCCGGATT
ATGGCAGAGATGCAAGCCCTATTCATGAACGGGACCGCAGTCGATCACCGGTGAGGCGGTCAGGGC

>TRINITY_DN45976_c0_g2_i1 from sample A8 / Example unspliced isoform:

TCCGGCGGTTAGTGAAGAAAAGTTTGCGATTAGCAATGAAGCCATTGTACTGTGGAAACATAGAGTACGAGGTTCGACAGTCAGAGATTGAGCGCTTGTTTGGCAAATATGGAAGAGTCGATAGGGTTGATATGAAATCAGGTAACTGCCATCATAGTATTCATATTCGCACCCAATATCTATCGATATATCTAGCATGT
ATTTATAATTGTATATTGCTGCAACAGATGTCTGTAAACTGGTTTAGCTGACAATATTTTGTCATTCAGATGGCTTTGGTTCCCAATGGTATAAACACTGTACAAACCATCCGTAGTTTGTCAAACCCCAAACGTGTTTGTTCTGGCAGGCTGTTTAAACCTGGCTCAAATCGATCTGAACAAATAAATAAATCCTGTTT
AGTCTTCTAGTCTCTGAAGAATTTCTATCTATATTTAGAAAACTTTAAATGATCTTGTAAGTTCAATCAGAAACGAATATTTTTAAGTAGGAAACCTAAGAGGATTTATCAGATTTGTTTTGTAAAATGTAAAAGACCCTGTGAAGTCTGAATTGTTCATGGACCATTGTTCTCAGGAGAGTTGAGTGATTTGGTTTTCC
AACAATGAACGCTTCCATACCCTTACCTGATTAATGGGTGAAGCCAGATTTGTGCTTCCAATGGCAAACTATTGTGCTCATTGGATGGAAATATCAACTATCTAAAGGAATGCAGACTTACTGTAAAAGATGCCCGGTGGCAGAAGTTCTAGCTCCAGCTATTATTCTTAATGGAATATAGTATGTCAACAAAATTATTT
TGATTTTAGAATTGGAAATCATGTCGAATGCATGATTTTCAGTCTTCTTTCCAGCTGGTAATGCGTAACGGTAACCTCTATGGTTCATATCCAAGGTTACAAGCAAAGTTTAAGAAGCAACATTTTTGAAATGTAGGCCTCTATACTCAAAAATTTCTTTGACGCAGGAATATTCAAGAGCATCCATGCTCTTCCTGGAG
ATCCTGTGCATATTCTTTCATCATGCCATGGATCAGCTAATAGCCTTGTTCAGCAAAGTTGATATACAAATGACATCTTGCATTTTTCTCACGACACCATTACTTTTCAATGTTCATCCCTCCCAAGCCATTCTAAGTCTTCATTCAGGCTCAAGCATTTCAAGAGATAGAATCCATGACACATCACTGCAATCAGAATG
GGGCATAACTGGTGACGGCACTTATCTGATGGATGACCTTACAGCCACTTATGGATTAGATTTACCTGATACCATTTAGTACAACATGCAACGTTTGTGCTTTCTGTCTCCTAATGCAGCCAGTATTTACGACAATAAGTAACAGgtgttgtatttttttttctttgtcgtagccttatatatgaaatcattcgccccag
cgcacactgcaaatgtgattgcaatccaatattttgttctttattgaatctcttgtttctatagGTTATGCTTTTGTCTATATGGAAGATGAGCGTGATGCAGAGGATGCAATCCGAGCTCTTGACAAGACAGAATTTGGCAGGCAAAGGCGTCGGCTAACTGTAGAGTGGACAAAGCATGCTGAACGAGGTCTTCGCCG
ATCTGAAGATGGCAGAAGATCTGTTACAGGTTTGAAGCCAACAAAGACATTATTTGTGATAAACTTTGACCCGTACGACACTAGAGTCAGGGACCTTGAAAAACATTTTGAGCCCTATGGTAAGGTGCTCAATGTAAGGATTAGAAGGAATTTTGCATTTATTCATTACGGATCACAAGAAGAAGCAACAAAAGCTTTCA
ATGCCACCCATATGAGCACATTATTAGATAGGGTAATCACAGTGGAATATGCTCAACGTGAGGATGGCAATAGAAGAGGTGGACCTTCTAGCCCCATTCGAGGAGGAGGTCGGTATGGCAGATCTGCTGAGGATCGTGGACGTGATCGGTCGGCAAGTCCTATATATGGGAGGCGTGCAATGAGGGGCAGTCCTGATTAT
GGTAGGGCTCCGAGTCCTATTTATTCAAGGCGTGCAGAGAGGCGCAGTCCTGACTACGGTAGGGCTCCAAGTCCTATATATGCAAGGCGTGCAGAGAGGCGCAGTCCTGTTTATTCCAGCCGCCCCCAAAAGAATAGTCCGGATTATGGCAGAGATGCAAGCCCTATTCATGAACGGGACCGCAGTCGATCACCGGTGAG
GCGGTCAGGGC

Intron 3 GTTAGTCGAA ... TGTGATTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	18.69	161	27	47
A2	16.99	127	33	19
A3	17.78	111	28	20
A4	17.13	75	10	21
A5	22.42	64	29	8
A6	13.41	494	61	92
A7	12.84	550	90	72
A8	14.93	507	88	90
A9	15.63	448	81	85
A10	13.38	453	69	71

>TRINITY_DN45976_c0_g2_i2 from sample A8 / Example spliced isoform

GTTGTATTTTTTTTTCTTTGTCGTAGCCTTATATATGAAATCATTCGCCCCAGCGCACACTGCAAATGTGATTGCAATCCAATATTTTGTTCTTTATTGAATCTCTTGTTTCTATAGGTTATGCTTTTGTCTATATGGAAGATGAGCGTGATGCAGAGGATGCAATCCGAGGGCTTGACAATACAGAATTTGGCAGACAA
AGACGTCGGCTAACTGTAGAGTGGACAAAGCATGCCGACCGAGGTCTTCGCCGATCTGAAGATGGCAGAAGATCTGTTACAGGTTTGAAGCCAACAAAGACATTATTTGTGATAAACTTTGACCCATACAACACCAGAACCAGGGACCTGGAAAGGCATTTTGAACCCTATGGTAAGGTGGTGAATGTGAGGATTAGAAG
GAATTTCGCATTTGTTCATTTTGAATCACAAGAAGAAGCAACGAAAGCTTTCGACTCTACTCATATGAGCACAATATTAGATAGGGTGATCACAGTGGAATATGCTCAACGTGAGGATGGCAATAGAAGAAATGGACGTTCCAGTCCCATTCGAGGAGGAGGTCGGTATGGCAGATCTGCTGAGGATCGTGGACGTGATC
GGTCGGCAAGTCCTATATATGGGAGGCGTGCAATGAGGGGCAGTCCTGATTATGGTAGGGCTCCGAGTCCTATTTATTCAAGGCGTGCAGAGAGGCGCAGTCCTGACTACGGTAGGGCTCCAAGTCCTATATATGCAAGGCGTGCAGAGAGGCGCAGTCCTGTTTATTCCAGCCGCCCCCAAAAGAATAGTCCGGATTAT
GGCAGAGATGCAAGCCCTATTCATGAACGGGACCGCAGTCGATCACCGGTGAGGCGGTCAGGGC

>TRINITY_DN45976_c0_g2_i9 from sample A8 / Example unspliced isoform:

GTTGTATTTTTTTTTCTTTGTCGTAGCCTTATATATGAAATCATTCGCCCCAGCGCACACTGCAAATGTGATTGCAATCCAATATTTTGTTCTTTATTGAATCTCTTGTTTCTATAGGTTATGCTTTTGTCTATATGGAAGATGAGCGTGATGCAGAGGATGCAATCCGAGGGCTTGACAATACAGAATTTGGCAGACAA
AGACGTCGGCTAACTGTAGAGTGGACAAAGCATGCCGACCGAGGTCTTCGCCGATCTGAAGATGGCAGAAGATCTGTTACAGGTTTGAAGCCAACAAAGACATTATTTGTGATAAACTTTGACCCATACAACACCAGAACCAGGGACCTGGAAAGGCATTTTGAACCCTATGGTAAGGTGGTGAATGTGAGGATTAGAAG
GAATTTCGCATTTGTTCATTTTGAATCACAAGAAGAAGCAACGAAAGCTTTCGACTCTACTCATATGAGgttagtcgaagttttcttatttgcaattcttgtaacttcctttccaaatgcaatgacgaatgtctccttcttattaaatctaaaattgtatgtgacgtttgtaccttgtactcgacttattgcatgggaca
ggattccgagtgttatttttgaagttccgcttagctggggaaatattaactagtccaaatgcggttttttatgttgctgatcttttattcatttctgtgattcagCACAATATTAGATAGGGTGATCACAGTGGAATATGCTCAACGTGAGGATGGCAATAGAAGAAATGGACGTTCCAGTCCCATTCGAGGAGGAGGTC
GGTATGGCAGATCTGCTGAGGATCGTGGACGTGATCGGTCGGCAAGTCCTATATATGGGAGGCGTGCAATGAGGGGCAGTCCTGATTATGGTAGGGCTCCGAGTCCTATTTATTCAAGGCGTGCAGAGAGGCGCAGTCCTGACTACGGTAGGGCTCCAAGTCCTATATATGCAAGGCGTGCAGAGAGGCGCAGTCCTGTT
TATTCCAGCCGCCCCCAAAAGAATAGTCCGGATTATGGCAGAGATGCAAGCCCTATTCATGAACGGGACCGCAGTCGATCACCGGTGAGGCGGTCAGGGC

Intron 4 GTAACTGCCA ... TAAGTAACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	15.14	171	34	27
A2	13.53	163	19	32
A3	14.9	197	45	24
A4	15.79	136	36	15
A5	10.62	101	13	11
A6	8.06	667	24	93
A7	9.69	857	58	126
A8	9.99	482	29	78
A9	8.97	751	23	125
A10	9.5	619	40	90

>TRINITY_DN45976_c0_g2_i5 from sample A8 / Example spliced isoform

TCCGGCGGTTAGTGAAGAAAAGTTTGCGATTAGCAATGAAGCCATTGTACTGTGGAAACATAGAGTACGAGGTTCGACAGTCAGAGATTGAGCGCTTGTTTGGCAAATATGGAAGAGTCGATAGGGTTGATATGAAATCAGGTTATGCTTTTGTCTATATGGAAGATGAGCGTGATGCAGAGGATGCAATCCGAGCTCTT
GACAAGACAGAATTTGGCAGGCAAAGGCGTCGGCTAACTGTAGAGTGGACAAAGCATGCTGAACGAGGTCTTCGCCGATCTGAAGATGGCAGAAGATCTGTTACAGGTTTGAAGCCAACAAAGACATTATTTGTGATAAACTTTGACCCGTACGACACTAGAGTCAGGGACCTTGAAAAACATTTTGAGCCCTATGGTAA
GGTGCTCAATGTAAGGATTAGAAGGAATTTTGCATTTATTCATTACGGATCACAAGAAGAAGCAACAAAAGCTTTCAATGCCACCCATATGAGCACATTATTAGATAGGGTAATCACAGTGGAATATGCTCAACGTGAGGATGGCAATAGAAGAGGTGGACCTTCTAGCCCCATTCGAGGAGGAGGTCGGTATGGCAGAT
CTGCTGAGGATCGTGGACGTGATCGGTCGGCAAGTCCTATATATGGGAGGCGTGCAATGAGGGGCAGTCCTGATTATGGTAGGGCTCCGAGTCCTATTTATTCAAGGCGTGCAGAGAGGCGCAGTCCTGACTACGGTAGGGCTCCAAGTCCTATATATGCAAGGCGTGCAGAGAGGCGCAGTCCTGTTTATTCCAGCCGC
CCCCAAAAGAATAGTCCGGATTATGGCAGAGATGCAAGCCCTATTCATGAACGGGACCGCAGTCGATCACCGGTGAGGCGGTCAGGGC

>TRINITY_DN45976_c0_g2_i8 from sample A8 / Example unspliced isoform:

TCCGGCGGTTAGTGAAGAAAAGTTTGCGATTAGCAATGAAGCCATTGTACTGTGGAAACATAGAGTACGAGGTTCGACAGTCAGAGATTGAGCGCTTGTTTGGCAAATATGGAAGAGTCGATAGGGTTGATATGAAATCAGgtaactgccatcatagtattcatattcgcacccaatatctatcgatatatctagcatgt
atttataattgtatattgctgcaacagatgtctgtaaactggtttagctgacaatattttgtcattcagatggctttggttcccaatggtataaacactgtacaaaccatccgtagtttgtcaaaccccaaacgtgtttgttctggcaggctgtttaaacctggctcaaatcgatctgaacaaataaataaatcctgttt
agtcttctagtctctgaagaatttctatctatatttagaaaactttaaatgatcttgtaagttcaatcagaaacgaatatttttaagtaggaaacctaagaggatttatcagatttgttttgtaaaatgtaaaagaccctgtgaagtctgaattgttcatggaccattgttctcaggagagttgagtgatttggttttcc
aacaatgaacgcttccatacccttacctgattaatgggtgaagccagatttgtgcttccaatggcaaactattgtgctcattggatggaaatatcaactatctaaaggaatgcagacttactgtaaaagatgcccggtggcagaagttctagctccagctattattcttaatggaatatagtatgtcaacaaaattattt
tgattttagaattggaaatcatgtcgaatgcatgattttcagtcttctttccagctggtaatgcgtaacggtaacctctatggttcatatccaaggttacaagcaaagtttaagaagcaacatttttgaaatgtaggcctctatactcaaaaatttctttgacgcaggaatattcaagagcatccatgctcttcctggag
atcctgtgcatattctttcatcatgccatggatcagctaatagccttgttcagcaaagttgatatacaaatgacatcttgcatttttctcacgacaccattacttttcaatgttcatccctcccaagccattctaagtcttcattcaggctcaagcatttcaagagatagaatccatgacacatcactgcaatcagaatg
gggcataactggtgacggcacttatctgatggatgaccttacagccacttatggattagatttacctgataccatttagtacaacatgcaacgtttgtgctttctgtctcctaatgcagccagtatttacgacaataagtaacagGTTATGCTTTTGTCTATATGGAAGATGAGCGTGATGCAGAGGATGCAATCCGAGC
TCTTGACAAGACAGAATTTGGCAGGCAAAGGCGTCGGCTAACTGTAGAGTGGACAAAGCATGCTGAACGAGGTCTTCGCCGATCTGAAGATGGCAGAAGATCTGTTACAGGTTTGAAGCCAACAAAGACATTATTTGTGATAAACTTTGACCCGTACGACACTAGAGTCAGGGACCTTGAAAAACATTTTGAGCCCTATG
GTAAGGTGCTCAATGTAAGGATTAGAAGGAATTTTGCATTTATTCATTACGGATCACAAGAAGAAGCAACAAAAGCTTTCAATGCCACCCATATGAGCACATTATTAGATAGGGTAATCACAGTGGAATATGCTCAACGTGAGGATGGCAATAGAAGAGGTGGACCTTCTAGCCCCATTCGAGGAGGAGGTCGGTATGGC
AGATCTGCTGAGGATCGTGGACGTGATCGGTCGGCAAGTCCTATATATGGGAGGCGTGCAATGAGGGGCAGTCCTGATTATGGTAGGGCTCCGAGTCCTATTTATTCAAGGCGTGCAGAGAGGCGCAGTCCTGACTACGGTAGGGCTCCAAGTCCTATATATGCAAGGCGTGCAGAGAGGCGCAGTCCTGTTTATTCCAG
CCGCCCCCAAAAGAATAGTCCGGATTATGGCAGAGATGCAAGCCCTATTCATGAACGGGACCGCAGTCGATCACCGGTGAGGCGGTCAGGGC

Intron 5 GTAACTGCCA ... GTTTCTATAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	14.07	171	34	22
A2	10.68	163	19	20
A3	13.41	197	45	16
A4	16.31	136	36	17
A5	9.42	101	13	8
A6	6.19	667	24	64
A7	7.65	857	58	84
A8	8.37	482	29	59
A9	6.3	751	23	78
A10	7.47	619	40	60

>TRINITY_DN45976_c0_g2_i5 from sample A8 / Example spliced isoform

TCCGGCGGTTAGTGAAGAAAAGTTTGCGATTAGCAATGAAGCCATTGTACTGTGGAAACATAGAGTACGAGGTTCGACAGTCAGAGATTGAGCGCTTGTTTGGCAAATATGGAAGAGTCGATAGGGTTGATATGAAATCAGGTTATGCTTTTGTCTATATGGAAGATGAGCGTGATGCAGAGGATGCAATCCGAGCTCTT
GACAAGACAGAATTTGGCAGGCAAAGGCGTCGGCTAACTGTAGAGTGGACAAAGCATGCTGAACGAGGTCTTCGCCGATCTGAAGATGGCAGAAGATCTGTTACAGGTTTGAAGCCAACAAAGACATTATTTGTGATAAACTTTGACCCGTACGACACTAGAGTCAGGGACCTTGAAAAACATTTTGAGCCCTATGGTAA
GGTGCTCAATGTAAGGATTAGAAGGAATTTTGCATTTATTCATTACGGATCACAAGAAGAAGCAACAAAAGCTTTCAATGCCACCCATATGAGCACATTATTAGATAGGGTAATCACAGTGGAATATGCTCAACGTGAGGATGGCAATAGAAGAGGTGGACCTTCTAGCCCCATTCGAGGAGGAGGTCGGTATGGCAGAT
CTGCTGAGGATCGTGGACGTGATCGGTCGGCAAGTCCTATATATGGGAGGCGTGCAATGAGGGGCAGTCCTGATTATGGTAGGGCTCCGAGTCCTATTTATTCAAGGCGTGCAGAGAGGCGCAGTCCTGACTACGGTAGGGCTCCAAGTCCTATATATGCAAGGCGTGCAGAGAGGCGCAGTCCTGTTTATTCCAGCCGC
CCCCAAAAGAATAGTCCGGATTATGGCAGAGATGCAAGCCCTATTCATGAACGGGACCGCAGTCGATCACCGGTGAGGCGGTCAGGGC

>TRINITY_DN45976_c0_g2_i1 from sample A8 / Example unspliced isoform:

TCCGGCGGTTAGTGAAGAAAAGTTTGCGATTAGCAATGAAGCCATTGTACTGTGGAAACATAGAGTACGAGGTTCGACAGTCAGAGATTGAGCGCTTGTTTGGCAAATATGGAAGAGTCGATAGGGTTGATATGAAATCAGgtaactgccatcatagtattcatattcgcacccaatatctatcgatatatctagcatgt
atttataattgtatattgctgcaacagatgtctgtaaactggtttagctgacaatattttgtcattcagatggctttggttcccaatggtataaacactgtacaaaccatccgtagtttgtcaaaccccaaacgtgtttgttctggcaggctgtttaaacctggctcaaatcgatctgaacaaataaataaatcctgttt
agtcttctagtctctgaagaatttctatctatatttagaaaactttaaatgatcttgtaagttcaatcagaaacgaatatttttaagtaggaaacctaagaggatttatcagatttgttttgtaaaatgtaaaagaccctgtgaagtctgaattgttcatggaccattgttctcaggagagttgagtgatttggttttcc
aacaatgaacgcttccatacccttacctgattaatgggtgaagccagatttgtgcttccaatggcaaactattgtgctcattggatggaaatatcaactatctaaaggaatgcagacttactgtaaaagatgcccggtggcagaagttctagctccagctattattcttaatggaatatagtatgtcaacaaaattattt
tgattttagaattggaaatcatgtcgaatgcatgattttcagtcttctttccagctggtaatgcgtaacggtaacctctatggttcatatccaaggttacaagcaaagtttaagaagcaacatttttgaaatgtaggcctctatactcaaaaatttctttgacgcaggaatattcaagagcatccatgctcttcctggag
atcctgtgcatattctttcatcatgccatggatcagctaatagccttgttcagcaaagttgatatacaaatgacatcttgcatttttctcacgacaccattacttttcaatgttcatccctcccaagccattctaagtcttcattcaggctcaagcatttcaagagatagaatccatgacacatcactgcaatcagaatg
gggcataactggtgacggcacttatctgatggatgaccttacagccacttatggattagatttacctgataccatttagtacaacatgcaacgtttgtgctttctgtctcctaatgcagccagtatttacgacaataagtaacaggtgttgtatttttttttctttgtcgtagccttatatatgaaatcattcgccccag
cgcacactgcaaatgtgattgcaatccaatattttgttctttattgaatctcttgtttctatagGTTATGCTTTTGTCTATATGGAAGATGAGCGTGATGCAGAGGATGCAATCCGAGCTCTTGACAAGACAGAATTTGGCAGGCAAAGGCGTCGGCTAACTGTAGAGTGGACAAAGCATGCTGAACGAGGTCTTCGCCG
ATCTGAAGATGGCAGAAGATCTGTTACAGGTTTGAAGCCAACAAAGACATTATTTGTGATAAACTTTGACCCGTACGACACTAGAGTCAGGGACCTTGAAAAACATTTTGAGCCCTATGGTAAGGTGCTCAATGTAAGGATTAGAAGGAATTTTGCATTTATTCATTACGGATCACAAGAAGAAGCAACAAAAGCTTTCA
ATGCCACCCATATGAGCACATTATTAGATAGGGTAATCACAGTGGAATATGCTCAACGTGAGGATGGCAATAGAAGAGGTGGACCTTCTAGCCCCATTCGAGGAGGAGGTCGGTATGGCAGATCTGCTGAGGATCGTGGACGTGATCGGTCGGCAAGTCCTATATATGGGAGGCGTGCAATGAGGGGCAGTCCTGATTAT
GGTAGGGCTCCGAGTCCTATTTATTCAAGGCGTGCAGAGAGGCGCAGTCCTGACTACGGTAGGGCTCCAAGTCCTATATATGCAAGGCGTGCAGAGAGGCGCAGTCCTGTTTATTCCAGCCGCCCCCAAAAGAATAGTCCGGATTATGGCAGAGATGCAAGCCCTATTCATGAACGGGACCGCAGTCGATCACCGGTGAG
GCGGTCAGGGC