sp|O22827|MOC2B_ARATH ----> Molybdopterin synthase catalytic subunit

Intron 1 GTTCTAGGGA ... ACGGATTTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	55.56	4	5	5
A2	33.33	8	6	2
A3	37.5	5	4	2
A4	-1	0	0	0
A5	0	1	0	0
A6	40.35	17	10	13
A7	51.02	24	16	34
A8	46.51	23	6	34
A9	48.03	33	16	45
A10	50	24	12	36

>TRINITY_DN39734_c0_g1_i2 from sample A8 / Example spliced isoform

CGCCCCATGACAACCCTAAAAGGTCAGAACAATCAATCCTTTTTTGGGTTGACACTTAGCCAACGAGAAAAACGCTCGTTTCCCCTTTCTCATGATTTAAATCAAAACATAAACTCAACTAAATTTAATCCTCCCGTCCCGTGGGAATTGAACCTGGAAACTCTAACCAAAAAAATTGCTCACAAATAGACAAAAAGCAA
AACTCTATTATGTTTCGTCGTCTCGCGTGTGGAATCCGAGAAAAGCTTGATGGAGGCGTTGAAGTGGCGAAATCTCGACTCGCATCATACGGCGTGCGGCATCCCTTCAGACCTACAAAGGACTCCGGGATCTATTTTATTCTGCAGTAATTCTCTGGACAGAAGGCATTTAAACATTCAAAGATCCTCCGTCGTGAGGA

GTCCAGCAGCCGGAGCAATAGCGACCTTCATTGGAACAACAAGGGATACGTTTGAGGATAAACAGGTTCTGGAGCTTAGATATGAGGCTTATGTCCCCATGGCCCTTAAACAGCTGAAAGCAATATGCTCTGCAGCTAGAAACCGATGGAAAGTAATGAGACTTGCAGTGGTGCATCGTCTCGGCGTTGTACCTGTTGGC
GAGGAAAGTGTTTTTGTTGCAGTGTCCTCTGTCCACAGGAGAGATGCTCTTGAAGCCTGTGAGTATATTATAGATGAAATCAAAGTATCAGTTCCTATTTGGAAAAAGGAAGTTTATACCAATGGTGAGGTCTGGAAGGAGAATTCTGAGTTTTTCCTGAGAAAACCAGTGGAAAGTGAAGAGGAGGTAAGTCTGAAACA
GCAGAAAGGTCATGAACGTTCTGGGTGCTGCAGCCGCAAGGAAAGAGTGTTTGAG

>TRINITY_DN39734_c0_g1_i4 from sample A8 / Example unspliced isoform:

CGCCCCATGACAACCCTAAAAGGTCAGAACAATCAATCCTTTTTTGGGTTGACACTTAGCCAACGAGAAAAACGCTCGTTTCCCCTTTCTCATGATTTAAATCAAAACATAAACTCAACTAAATTTAATCCTCCCGTCCCGTGGGAATTGAACCTGGAAACTCTAACCAAAAAAATTGCTCACAAATAGACAAAAAGCAA
AACTCTATTATGTTTCGTCGTCTCGCGTGTGGAATCCGAGAAAAGCTTGATGGAGGCGTTGAAGTGGCGAAATCTCGACTCGCATCATACGGCGTGCGGCATCCCTTCAGACCTACAAAGGACTCCGGGATCTATTTTATTCTGCAGTAATTCTCTGGACAGAAGGCATTTAAACATTCAAAGATCCTCCGTCGgttcta
gggaactggtattgtggttattcttacagattttgtgtaaattgtgctgcggagattcttaaggttcagctattttgtggccaaaactttaaaatattggtataggtggctccttaatagttgagaccccaactgttgagattatttaatgtcagcttccaggaaaaagattttgtggttattcctgtaggttgtgtaaa
ttgtgctgagaaggttctggaggcttagctactttggtgtgaagtcatagaaaattagtatattttactccttgaaagaatggctaatggagaacaagattttgttgaaattgttgatgattctcacttggatattggaaaatatacggatttagTGAGGAGTCCAGCAGCCGGAGCAATAGCGACCTTCATTGGAACAA
CAAGGGATACGTTTGAGGATAAACAGGTTCTGGAGCTTAGATATGAGGCTTATGTCCCCATGGCCCTTAAACAGCTGAAAGCAATATGCTCTGCAGCTAGAAACCGATGGAAAGTAATGAGACTTGCAGTGGTGCATCGTCTCGGCGTTGTACCTGTTGGCGAGGAAAGTGTTTTTGTTGCAGTGTCCTCTGTCCACAGG
AGAGATGCTCTTGAAGCCTGTGAGTATATTATAGATGAAATCAAAGTATCAGTTCCTATTTGGAAAAAGGAAGTTTATACCAATGGTGAGGTCTGGAAGGAGAATTCTGAGTTTTTCCTGAGAAAACCAGTGGAAAGTGAAGAGGAGGTAAGTCTGAAACAGCAGAAAGGTCATGAACGTTCTGGGTGCTGCAGCCGCAA
GGAAAGAGTGTTTGAG

Intron 2 GTATGTTTTG ... ATTCCTGTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	38.46	4	4	1
A2	58.62	6	13	4
A3	61.9	4	5	8
A4	75	1	2	4
A5	42.86	4	2	4
A6	62.5	9	22	8
A7	58.9	15	18	25
A8	67.44	7	17	12
A9	50	15	21	9
A10	53.85	12	17	11

>TRINITY_DN40130_c0_g1_i2 from sample A7 / Example spliced isoform

TGGGAATTGAACCTGGAAACTCTAACCAAAAAAATTGCTCACAAATAGACAAAAAGCAAAACTCTATTATGTTTCGTCGTCTCGCGTGTGGAATCCGAGAAAAGCTTGATGGAGGCGTTGAAGTGGCGAAATCTCGACTCGCATCATACGGCGTGCGGCATCCCTTCAGACCTACAAAGGACTCCGGGATCTATTTTATT
CTGCAGTAATTCTCTGGACAGAAGGCATTTAAACATTCAAAGATCCTCCGTCGGTTGTGTAAATTGTGCTGAGAAGGTTCTGGAGGCTTAGCTACTTTGGTGTGAAGTCATAGAAAATTAGTATATTTTACTCCTTGAAAGAATGGCTAATGGAGAACAAGATTTTGTTGAAATTGTTGATGATTCTCACTTGGATATTG
GAAAATATACGGATTTAGTGAGGAGTCCAGCAGCCGGAGCAATAGCGACCTTCATTGGAACAACAAGGGATACGTTTGAGGATAAACAGGTTCTGGAGCTTAGATATGAGGCTTATGTCCCCATGGCCCTTAAACAGCTGAAAGCAATATGCTCTGCAGCTAGAAACCGATGGAAAGTAATGAGACTTGCAGTGGTGCAT
CGTCTCGGCGTTGTACCTGTTGGCGAGGAAAGTGTTTTTGTTGCAGTGTCCTCTGTCCACAGGAGAGATGCTCTTGAAGCCTGTGAGTATATTATAGATGAAATCAAAGTATCAGTTCCTATTTGGAAAAAGGAAGTTTATACCAATGGTGAGGTCTGGAAGGAGAATTCTGAGTTTTTCCTGAGAAAACCAGTGGAAAG
TGAAGAGGAGGTAAGTCTGAAACAGCAGAAAGGTCATGAACGTTCTGGGTGCTGCAGCCGCAAGGAAAGAGTGTTTGAG

>TRINITY_DN40130_c0_g1_i3 from sample A7 / Example unspliced isoform:

TGGGAATTGAACCTGGAAACTCTAACCAAAAAAATTGCTCACAAATAGACAAAAAGCAAAACTCTATTATGTTTCGTCGTCTCGCGTGTGGAATCCGAGAAAAGCTTGATGGAGGCGTTGAAGTGGCGAAATCTCGACTCGCATCATACGGCGTGCGGCATCCCTTCAGACCTACAAAGGACTCCGGGATCTATTTTATT
CTGCAGTAATTCTCTGGACAGAAGGCATTTAAACATTCAAAGATCCTCCGTCGgtatgttttgcctgtctttaacgcaaacaatgcacatgacctaggcttcgagccagaaaattttctgtgaagaaatatattaagttgtatttgtttatttggaataagaatttgatgttttcttttgcggtggaaattttctttctt
tagaccttttctgattctcttgagctcattctttgtatttgcacggctgtaaatctggaatctatctcacagggcccttccttttttcagctttttcctccagttgactataatgttgtgctattttaaaatatttttttgtttattttgtcctgttttaaaagcatttaaagtttagttagtattcggattgacggaaa
atattttagtaatactatttgaaacatcgaatagctttaacgattagttaaatgaagcatcttcatttctgcgttttgcagacagagttcttcttttctggtgagcatttagctgtagaaaaacagttctctctgtatattctattgtttctttgaatgattttcgcatgaacaatcgccctttttaaattttaagttat
ttgaatgatttgctttacgcggttatcattgttagatagaaaatattttgtttgcaatttttagacggttacttactggtcttattttttccttgtactgaaattccccactctaaactctttccccagagaaatgcatgaattatttctgtatgtttttgtctttggaagccaatattgagaatatttaatgccaggtt
ctagggaactggtattgtggttattcttacagattttgtgtaaattgtgctgcggagattcttaaggttcagctattttgtggccaaaactttaaaatattggtataggtggctccttaatagttgagaccccaactgttgagattatttaatgtcagcttccaggaaaaagattttgtggttattcctgtagGTTGTGT
AAATTGTGCTGAGAAGGTTCTGGAGGCTTAGCTACTTTGGTGTGAAGTCATAGAAAATTAGTATATTTTACTCCTTGAAAGAATGGCTAATGGAGAACAAGATTTTGTTGAAATTGTTGATGATTCTCACTTGGATATTGGAAAATATACGGATTTAGTGAGGAGTCCAGCAGCCGGAGCAATAGCGACCTTCATTGGAA
CAACAAGGGATACGTTTGAGGATAAACAGGTTCTGGAGCTTAGATATGAGGCTTATGTCCCCATGGCCCTTAAACAGCTGAAAGCAATATGCTCTGCAGCTAGAAACCGATGGAAAGTAATGAGACTTGCAGTGGTGCATCGTCTCGGCGTTGTACCTGTTGGCGAGGAAAGTGTTTTTGTTGCAGTGTCCTCTGTCCAC
AGGAGAGATGCTCTTGAAGCCTGTGAGTATATTATAGATGAAATCAAAGTATCAGTTCCTATTTGGAAAAAGGAAGTTTATACCAATGGTGAGGTCTGGAAGGAGAATTCTGAGTTTTTCCTGAGAAAACCAGTGGAAAGTGAAGAGGAGGTAAGTCTGAAACAGCAGAAAGGTCATGAACGTTCTGGGTGCTGCAGCCG
CAAGGAAAGAGTGTTTGAG

Intron 3 GTATGTTTTG ... ACGGATTTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	52.94	4	4	5
A2	48.39	8	13	2
A3	41.18	5	5	2
A4	100	0	2	0
A5	50	1	2	0
A6	50.72	17	22	13
A7	52	24	18	34
A8	52.58	23	17	34
A9	50	33	21	45
A10	52.48	24	17	36

>TRINITY_DN40130_c0_g1_i1 from sample A7 / Example spliced isoform

TGGGAATTGAACCTGGAAACTCTAACCAAAAAAATTGCTCACAAATAGACAAAAAGCAAAACTCTATTATGTTTCGTCGTCTCGCGTGTGGAATCCGAGAAAAGCTTGATGGAGGCGTTGAAGTGGCGAAATCTCGACTCGCATCATACGGCGTGCGGCATCCCTTCAGACCTACAAAGGACTCCGGGATCTATTTTATT
CTGCAGTAATTCTCTGGACAGAAGGCATTTAAACATTCAAAGATCCTCCGTCGTGAGGAGTCCAGCAGCCGGAGCAATAGCGACCTTCATTGGAACAACAAGGGATACGTTTGAGGATAAACAGGTTCTGGAGCTTAGATATGAGGCTTATGTCCCCATGGCCCTTAAACAGCTGAAAGCAATATGCTCTGCAGCTAGAA
ACCGATGGAAAGTAATGAGACTTGCAGTGGTGCATCGTCTCGGCGTTGTACCTGTTGGCGAGGAAAGTGTTTTTGTTGCAGTGTCCTCTGTCCACAGGAGAGATGCTCTTGAAGCCTGTGAGTATATTATAGATGAAATCAAAGTATCAGTTCCTATTTGGAAAAAGGAAGTTTATACCAATGGTGAGGTCTGGAAGGAG
AATTCTGAGTTTTTCCTGAGAAAACCAGTGGAAAGTGAAGAGGAGGTAAGTCTGAAACAGCAGAAAGGTCATGAACGTTCTGGGTGCTGCAGCCGCAAGGAAAGAGTGTTTGAG

>TRINITY_DN40130_c0_g1_i3 from sample A7 / Example unspliced isoform:

TGGGAATTGAACCTGGAAACTCTAACCAAAAAAATTGCTCACAAATAGACAAAAAGCAAAACTCTATTATGTTTCGTCGTCTCGCGTGTGGAATCCGAGAAAAGCTTGATGGAGGCGTTGAAGTGGCGAAATCTCGACTCGCATCATACGGCGTGCGGCATCCCTTCAGACCTACAAAGGACTCCGGGATCTATTTTATT
CTGCAGTAATTCTCTGGACAGAAGGCATTTAAACATTCAAAGATCCTCCGTCGgtatgttttgcctgtctttaacgcaaacaatgcacatgacctaggcttcgagccagaaaattttctgtgaagaaatatattaagttgtatttgtttatttggaataagaatttgatgttttcttttgcggtggaaattttctttctt
tagaccttttctgattctcttgagctcattctttgtatttgcacggctgtaaatctggaatctatctcacagggcccttccttttttcagctttttcctccagttgactataatgttgtgctattttaaaatatttttttgtttattttgtcctgttttaaaagcatttaaagtttagttagtattcggattgacggaaa
atattttagtaatactatttgaaacatcgaatagctttaacgattagttaaatgaagcatcttcatttctgcgttttgcagacagagttcttcttttctggtgagcatttagctgtagaaaaacagttctctctgtatattctattgtttctttgaatgattttcgcatgaacaatcgccctttttaaattttaagttat
ttgaatgatttgctttacgcggttatcattgttagatagaaaatattttgtttgcaatttttagacggttacttactggtcttattttttccttgtactgaaattccccactctaaactctttccccagagaaatgcatgaattatttctgtatgtttttgtctttggaagccaatattgagaatatttaatgccaggtt
ctagggaactggtattgtggttattcttacagattttgtgtaaattgtgctgcggagattcttaaggttcagctattttgtggccaaaactttaaaatattggtataggtggctccttaatagttgagaccccaactgttgagattatttaatgtcagcttccaggaaaaagattttgtggttattcctgtaggttgtgt
aaattgtgctgagaaggttctggaggcttagctactttggtgtgaagtcatagaaaattagtatattttactccttgaaagaatggctaatggagaacaagattttgttgaaattgttgatgattctcacttggatattggaaaatatacggatttagTGAGGAGTCCAGCAGCCGGAGCAATAGCGACCTTCATTGGAA
CAACAAGGGATACGTTTGAGGATAAACAGGTTCTGGAGCTTAGATATGAGGCTTATGTCCCCATGGCCCTTAAACAGCTGAAAGCAATATGCTCTGCAGCTAGAAACCGATGGAAAGTAATGAGACTTGCAGTGGTGCATCGTCTCGGCGTTGTACCTGTTGGCGAGGAAAGTGTTTTTGTTGCAGTGTCCTCTGTCCAC
AGGAGAGATGCTCTTGAAGCCTGTGAGTATATTATAGATGAAATCAAAGTATCAGTTCCTATTTGGAAAAAGGAAGTTTATACCAATGGTGAGGTCTGGAAGGAGAATTCTGAGTTTTTCCTGAGAAAACCAGTGGAAAGTGAAGAGGAGGTAAGTCTGAAACAGCAGAAAGGTCATGAACGTTCTGGGTGCTGCAGCCG
CAAGGAAAGAGTGTTTGAG

Intron 4 GTATGTTTTG ... TTAATGCCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	44.44	5	4	3
A2	68.42	6	13	1
A3	55.56	4	5	2
A4	100	0	2	1
A5	100	0	2	2
A6	68.75	10	22	1
A7	52.94	16	18	5
A8	73.91	6	17	4
A9	56.76	16	21	3
A10	58.62	12	17	4

>TRINITY_DN39734_c0_g1_i4 from sample A8 / Example spliced isoform

CGCCCCATGACAACCCTAAAAGGTCAGAACAATCAATCCTTTTTTGGGTTGACACTTAGCCAACGAGAAAAACGCTCGTTTCCCCTTTCTCATGATTTAAATCAAAACATAAACTCAACTAAATTTAATCCTCCCGTCCCGTGGGAATTGAACCTGGAAACTCTAACCAAAAAAATTGCTCACAAATAGACAAAAAGCAA
AACTCTATTATGTTTCGTCGTCTCGCGTGTGGAATCCGAGAAAAGCTTGATGGAGGCGTTGAAGTGGCGAAATCTCGACTCGCATCATACGGCGTGCGGCATCCCTTCAGACCTACAAAGGACTCCGGGATCTATTTTATTCTGCAGTAATTCTCTGGACAGAAGGCATTTAAACATTCAAAGATCCTCCGTCGGTTCTA

GGGAACTGGTATTGTGGTTATTCTTACAGATTTTGTGTAAATTGTGCTGCGGAGATTCTTAAGGTTCAGCTATTTTGTGGCCAAAACTTTAAAATATTGGTATAGGTGGCTCCTTAATAGTTGAGACCCCAACTGTTGAGATTATTTAATGTCAGCTTCCAGGAAAAAGATTTTGTGGTTATTCCTGTAGGTTGTGTAAA
TTGTGCTGAGAAGGTTCTGGAGGCTTAGCTACTTTGGTGTGAAGTCATAGAAAATTAGTATATTTTACTCCTTGAAAGAATGGCTAATGGAGAACAAGATTTTGTTGAAATTGTTGATGATTCTCACTTGGATATTGGAAAATATACGGATTTAGTGAGGAGTCCAGCAGCCGGAGCAATAGCGACCTTCATTGGAACAA
CAAGGGATACGTTTGAGGATAAACAGGTTCTGGAGCTTAGATATGAGGCTTATGTCCCCATGGCCCTTAAACAGCTGAAAGCAATATGCTCTGCAGCTAGAAACCGATGGAAAGTAATGAGACTTGCAGTGGTGCATCGTCTCGGCGTTGTACCTGTTGGCGAGGAAAGTGTTTTTGTTGCAGTGTCCTCTGTCCACAGG
AGAGATGCTCTTGAAGCCTGTGAGTATATTATAGATGAAATCAAAGTATCAGTTCCTATTTGGAAAAAGGAAGTTTATACCAATGGTGAGGTCTGGAAGGAGAATTCTGAGTTTTTCCTGAGAAAACCAGTGGAAAGTGAAGAGGAGGTAAGTCTGAAACAGCAGAAAGGTCATGAACGTTCTGGGTGCTGCAGCCGCAA
GGAAAGAGTGTTTGAG

>TRINITY_DN39734_c0_g1_i3 from sample A8 / Example unspliced isoform:

CGCCCCATGACAACCCTAAAAGGTCAGAACAATCAATCCTTTTTTGGGTTGACACTTAGCCAACGAGAAAAACGCTCGTTTCCCCTTTCTCATGATTTAAATCAAAACATAAACTCAACTAAATTTAATCCTCCCGTCCCGTGGGAATTGAACCTGGAAACTCTAACCAAAAAAATTGCTCACAAATAGACAAAAAGCAA
AACTCTATTATGTTTCGTCGTCTCGCGTGTGGAATCCGAGAAAAGCTTGATGGAGGCGTTGAAGTGGCGAAATCTCGACTCGCATCATACGGCGTGCGGCATCCCTTCAGACCTACAAAGGACTCCGGGATCTATTTTATTCTGCAGTAATTCTCTGGACAGAAGGCATTTAAACATTCAAAGATCCTCCGTCGgtatgt
tttgcctgtctttaacgcaaacaatgcacatgacctaggcttcgagccagaaaattttctgtgaagaaatatattaagttgtatttgtttatttggaataagaatttgatgttttcttttgcggtggaaattttctttctttagaccttttctgattctcttgagctcattctttgtatttgcacggctgtaaatctgga
atctatctcacagggcccttccttttttcagctttttcctccagttgactataatgttgtgctattttaaaatatttttttgtttattttgtcctgttttaaaagcatttaaagtttagttagtattcggattgacggaaaatattttagtaatactatttgaaacatcgaatagctttaacgattagttaaatgaagca
tcttcatttctgcgttttgcagacagagttcttcttttctggtgagcatttagctgtagaaaaacagttctctctgtatattctattgtttctttgaatgattttcgcatgaacaatcgccctttttaaattttaagttatttgaatgatttgctttacgcggttatcattgttagatagaaaatattttgtttgcaatt
tttagacggttacttactggtcttattttttccttgtactgaaattccccactctaaactctttccccagagaaatgcatgaattatttctgtatgtttttgtctttggaagccaatattgagaatatttaatgccagGTTCTAGGGAACTGGTATTGTGGTTATTCTTACAGATTTTGTGTAAATTGTGCTGCGGAGAT
TCTTAAGGTTCAGCTATTTTGTGGCCAAAACTTTAAAATATTGGTATAGGTGGCTCCTTAATAGTTGAGACCCCAACTGTTGAGATTATTTAATGTCAGCTTCCAGGAAAAAGATTTTGTGGTTATTCCTGTAGGTTGTGTAAATTGTGCTGAGAAGGTTCTGGAGGCTTAGCTACTTTGGTGTGAAGTCATAGAAAATT
AGTATATTTTACTCCTTGAAAGAATGGCTAATGGAGAACAAGATTTTGTTGAAATTGTTGATGATTCTCACTTGGATATTGGAAAATATACGGATTTAGTGAGGAGTCCAGCAGCCGGAGCAATAGCGACCTTCATTGGAACAACAAGGGATACGTTTGAGGATAAACAGGTTCTGGAGCTTAGATATGAGGCTTATGTC
CCCATGGCCCTTAAACAGCTGAAAGCAATATGCTCTGCAGCTAGAAACCGATGGAAAGTAATGAGACTTGCAGTGGTGCATCGTCTCGGCGTTGTACCTGTTGGCGAGGAAAGTGTTTTTGTTGCAGTGTCCTCTGTCCACAGGAGAGATGCTCTTGAAGCCTGTGAGTATATTATAGATGAAATCAAAGTATCAGTTCC
TATTTGGAAAAAGGAAGTTTATACCAATGGTGAGGTCTGGAAGGAGAATTCTGAGTTTTTCCTGAGAAAACCAGTGGAAAGTGAAGAGGAGGTAAGTCTGAAACAGCAGAAAGGTCATGAACGTTCTGGGTGCTGCAGCCGCAAGGAAAGAGTGTTTGAG

Intron 5 GTTCTAGGGA ... ATTCCTGTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	42.86	4	5	1
A2	45.45	6	6	4
A3	60	4	4	8
A4	66.67	1	0	4
A5	33.33	4	0	4
A6	50	9	10	8
A7	57.75	15	16	25
A8	56.25	7	6	12
A9	45.45	15	16	9
A10	48.94	12	12	11

>TRINITY_DN37834_c1_g1_i10 from sample A9 / Example spliced isoform

CTCAACTAAATTTAATCCTCCCGTCCCGTGGGAATTGAACCTGGAAACTCTAACCAAAAAAATTGCTCACAAATAGACAAAAAGCAAAACTCTATTATGTTTCGTCGTCTCGCGTGTGGAATCCGAGAAAAGCTTGATGGAGGCGTTGAAGTGGCGAAATCTCGACTCGCATCATACGGCGTGCGGCATCCCTTCAGACC
TACAAAGGACTCCGGGATCTATTTTATTCTGCAGTAATTCTCTGGACAGAAGGCATTTAAACATTCAAAGATCCTCCGTCGGTTGTGTAAATTGTGCTGAGAAGGTTCTGGAGGCTTAGCTACTTTGGTGTGAAGTCATAGAAAATTAGTATATTTTACTCCTTGAAAGAATGGCTAATGGAGAACAAGATTTTGTTGAA
ATTGTTGATGATTCTCACTTGGATATTGGAAAATATACGGATTTAGTGAGGAGTCCAGCAGCCGGAGCAATAGCGACCTTCATTGGAACAACAAGGGATACGTTTGAGGATAAACAGGTTCTGGAGCTTAGATATGAGGCTTATGTCCCCATGGCCCTTAAACAGCTGAAAGCAATATGCTCTGCAGCTAGAAACCGATG
GAAAGTAATGAGACTTGCAGTGGTGCATCGTCTCGGCGTTGTACCTGTTGGCGAGGAAAGTGTTTTTGTTGCAGTGTCCTCTGTCCACAGGAGAGATGCTCTTGAAGCCTGTGAGTATATTATAGATGAAATCAAAGTATCAGTTCCTATTTGGAAAAAGGAAGTTTATACCAATGGTGAGGTCTGGAAGGAGAATTCTG
AGTTTTTCCTGAGAAAACCAGTGGAAAGTGAAGAGGAGGTAAGTCTGAAACAGCAGAAAGGTCATGAACGTTCTGGGTGCTGCAGCCGCAAGGAAAGAGTGTTTGAGAATGGTAGTGAAGTCTGGAAGGAGAATTCTGAGTTTTTCCTGAGAAAACCAGTGGAAAGTGAAGAGGATGTAAGTCTGAAACAGCAGAAAGGT
CATGAACGTTCTGGGTGCTGCAGCCGCAAGGAAAGAGTGTTTGAGAATGGTAGTGAGGTCTGGAAGGAGAATTCTGAGTTTTTCCTGAGAAAACCAGTGGAAAGTGAAGAGGATGTAAGTCTGAAACAGCAGAAAGGTCATGAACGTTCTGGGTGCTGCAGCCGCAAGGAAAGAGTGTTTGAGAATGGTAGTGAGGACAA
TAGCCATTGCAGATTCGAA

>TRINITY_DN37834_c1_g1_i9 from sample A9 / Example unspliced isoform:

CTCAACTAAATTTAATCCTCCCGTCCCGTGGGAATTGAACCTGGAAACTCTAACCAAAAAAATTGCTCACAAATAGACAAAAAGCAAAACTCTATTATGTTTCGTCGTCTCGCGTGTGGAATCCGAGAAAAGCTTGATGGAGGCGTTGAAGTGGCGAAATCTCGACTCGCATCATACGGCGTGCGGCATCCCTTCAGACC
TACAAAGGACTCCGGGATCTATTTTATTCTGCAGTAATTCTCTGGACAGAAGGCATTTAAACATTCAAAGATCCTCCGTCGgttctagggaactggtattgtggttattcttacagattttgtgtaaattgtgctgcggagattcttaaggttcagctattttgtggccaaaactttaaaatattggtataggtggctcc
ttaatagttgagaccccaactgttgagattatttaatgtcagcttccaggaaaaagattttgtggttattcctgtagGTTGTGTAAATTGTGCTGAGAAGGTTCTGGAGGCTTAGCTACTTTGGTGTGAAGTCATAGAAAATTAGTATATTTTACTCCTTGAAAGAATGGCTAATGGAGAACAAGATTTTGTTGAAATTG
TTGATGATTCTCACTTGGATATTGGAAAATATACGGATTTAGTGAGGAGTCCAGCAGCCGGAGCAATAGCGACCTTCATTGGAACAACAAGGGATACGTTTGAGGATAAACAGGTTCTGGAGCTTAGATATGAGGCTTATGTCCCCATGGCCCTTAAACAGCTGAAAGCAATATGCTCTGCAGCTAGAAACCGATGGAAA
GTAATGAGACTTGCAGTGGTGCATCGTCTCGGCGTTGTACCTGTTGGCGAGGAAAGTGTTTTTGTTGCAGTGTCCTCTGTCCACAGGAGAGATGCTCTTGAAGCCTGTGAGTATATTATAGATGAAATCAAAGTATCAGTTCCTATTTGGAAAAAGGAAGTTTATACCAATGGTGAGGTCTGGAAGGAGAATTCTGAGTT
TTTCCTGAGAAAACCAGTGGAAAGTGAAGAGGAGGTAAGTCTGAAACAGCAGAAAGGTCATGAACGTTCTGGGTGCTGCAGCCGCAAGGAAAGAGTGTTTGAGAATGGTAGTGAAGTCTGGAAGGAGAATTCTGAGTTTTTCCTGAGAAAACCAGTGGAAAGTGAAGAGGATGTAAGTCTGAAACAGCAGAAAGGTCATG
AACGTTCTGGGTGCTGCAGCCGCAAGGAAAGAGTGTTTGAGAATGGTAGTGAGGTCTGGAAGGAGAATTCTGAGTTTTTCCTGAGAAAACCAGTGGAAAGTGAAGAGGATGTAAGTCTGAAACAGCAGAAAGGTCATGAACGTTCTGGGTGCTGCAGCCGCAAGGAAAGAGTGTTTGAGAATGGTAGTGAGGACAATAGC
CATTGCAGATTCGAA

Intron 6 GTTGTGTAAA ... ACGGATTTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	52.94	4	4	5
A2	33.33	8	6	2
A3	37.5	5	4	2
A4	100	0	1	0
A5	66.67	1	4	0
A6	39.29	17	9	13
A7	50.52	24	15	34
A8	47.13	23	7	34
A9	47.62	33	15	45
A10	50	24	12	36

>TRINITY_DN40130_c0_g1_i1 from sample A7 / Example spliced isoform

TGGGAATTGAACCTGGAAACTCTAACCAAAAAAATTGCTCACAAATAGACAAAAAGCAAAACTCTATTATGTTTCGTCGTCTCGCGTGTGGAATCCGAGAAAAGCTTGATGGAGGCGTTGAAGTGGCGAAATCTCGACTCGCATCATACGGCGTGCGGCATCCCTTCAGACCTACAAAGGACTCCGGGATCTATTTTATT
CTGCAGTAATTCTCTGGACAGAAGGCATTTAAACATTCAAAGATCCTCCGTCGTGAGGAGTCCAGCAGCCGGAGCAATAGCGACCTTCATTGGAACAACAAGGGATACGTTTGAGGATAAACAGGTTCTGGAGCTTAGATATGAGGCTTATGTCCCCATGGCCCTTAAACAGCTGAAAGCAATATGCTCTGCAGCTAGAA
ACCGATGGAAAGTAATGAGACTTGCAGTGGTGCATCGTCTCGGCGTTGTACCTGTTGGCGAGGAAAGTGTTTTTGTTGCAGTGTCCTCTGTCCACAGGAGAGATGCTCTTGAAGCCTGTGAGTATATTATAGATGAAATCAAAGTATCAGTTCCTATTTGGAAAAAGGAAGTTTATACCAATGGTGAGGTCTGGAAGGAG
AATTCTGAGTTTTTCCTGAGAAAACCAGTGGAAAGTGAAGAGGAGGTAAGTCTGAAACAGCAGAAAGGTCATGAACGTTCTGGGTGCTGCAGCCGCAAGGAAAGAGTGTTTGAG

>TRINITY_DN40130_c0_g1_i2 from sample A7 / Example unspliced isoform:

TGGGAATTGAACCTGGAAACTCTAACCAAAAAAATTGCTCACAAATAGACAAAAAGCAAAACTCTATTATGTTTCGTCGTCTCGCGTGTGGAATCCGAGAAAAGCTTGATGGAGGCGTTGAAGTGGCGAAATCTCGACTCGCATCATACGGCGTGCGGCATCCCTTCAGACCTACAAAGGACTCCGGGATCTATTTTATT
CTGCAGTAATTCTCTGGACAGAAGGCATTTAAACATTCAAAGATCCTCCGTCGgttgtgtaaattgtgctgagaaggttctggaggcttagctactttggtgtgaagtcatagaaaattagtatattttactccttgaaagaatggctaatggagaacaagattttgttgaaattgttgatgattctcacttggatattg
gaaaatatacggatttagTGAGGAGTCCAGCAGCCGGAGCAATAGCGACCTTCATTGGAACAACAAGGGATACGTTTGAGGATAAACAGGTTCTGGAGCTTAGATATGAGGCTTATGTCCCCATGGCCCTTAAACAGCTGAAAGCAATATGCTCTGCAGCTAGAAACCGATGGAAAGTAATGAGACTTGCAGTGGTGCAT
CGTCTCGGCGTTGTACCTGTTGGCGAGGAAAGTGTTTTTGTTGCAGTGTCCTCTGTCCACAGGAGAGATGCTCTTGAAGCCTGTGAGTATATTATAGATGAAATCAAAGTATCAGTTCCTATTTGGAAAAAGGAAGTTTATACCAATGGTGAGGTCTGGAAGGAGAATTCTGAGTTTTTCCTGAGAAAACCAGTGGAAAG
TGAAGAGGAGGTAAGTCTGAAACAGCAGAAAGGTCATGAACGTTCTGGGTGCTGCAGCCGCAAGGAAAGAGTGTTTGAG