NA ----> NA

Intron 1 GTAGAAAAGG ... CTGCTTGAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	78.59	25	70	113.5
A2	80.43	23	72	117
A3	76.3	25	61	100
A4	80.86	20	70	99
A5	82.98	8	33	45
A6	85.28	12	56	83
A7	87.74	26	149	223
A8	84.95	21	96	141
A9	84.5	21	84	145
A10	80.61	19	66	92

>TRINITY_DN118749_c0_g1_i3 from sample A3 / Example spliced isoform

TGGACCCCTTTAGCAAGCCGCTGTTGCTTCTTCCACCAGCCTACATTCATCAGTCATTCATTCCCTTTAGCCTTTGCTTCTGATACTTGATCCTCGCTACAACAACAGCTATTGATTTTGATTTCCCTATCAAAATAAAAGGTCGCAGGTTCGAGTTCTCAGAAGCGGGAGCACAGACGTACATACAATCTTAAAGATAT
AGCTTGCTGATACTAAGGAAATATAGGGTTTACTACACAATCTTGTCAAAACGGCGTTGAGGCGAGAAAATCTGCGTTACAGGTTGACTTCAGCAAGGTCGATTGAAGAGACAGGACTAGATTCTTTCTCTTATGGGGACGGCTTCTCATATTATCCTGCTCGTGCAACAATTCCAGCCCCTCAAGAGGGCTTCAACACT
GCTCCAAAGCTTAATACAAAAACAATGACAAGTAGGGATGCATACCTTGCTAGACAAGGTGCCTATCGAGATGTCCATTTTGCACACCAGCAGAGCTTGGTTCCAGATTATGAGGAGAAACACAGCTTCATAGATGGTATCCCTGAAATGCAGGCTAGTGGGGAGAAGGATGATCCTTTTGAGAATGGACAAACTTGGAA
TGAGAAAAGGAGAGTGAGAAGAAGTAATAGCAAAAATGGAAGATTAGGCAATTTCAAGAAATTGCAAGAGAAGGAATTTTGCTTTGCAGATTCTGCTGTAAAGGATGAAAGAGGCTGCACCACAGTTTTACCTGTCGATGGAAAATATATTGAGAATAACAATTTCCTTCCATCAAACAATAGGAAGATCAAGGCAAAGC
GGCTTCTGACCAACTCACATATGCAGGATAAAAATACAGAGACAAAATTGGATAACCATTCAAATGAATCTGCTGAGAATGTCATGTTCACTAAACATGACTCTACTTCTGAGCAACAGGATGAAAGACAACAATGCAGTTTGACCACTGGGGAAGCAGATAGTGTTACATTTTCATTGGTCACTTCGAAACATAACAAT
ACTACTGAGGGTGCTAAACTAATGCATACCTCTATGGATCAGTTGTTAAATGATACCTCTCCAACTTGCTCCAAGACTGTGTTTGAACAATCTTATCTTATATCTAATGCAAAGCTTTCATCATCTGGCACTCATACAGACGATTGCATAGATGGTTTGCCAACACAAAAAAATGATGTATCTTCATGTGAGCCATCCCC
AGCCAGCAACCAGAATGAGATTGTTGCAGATTCCTGTTTACCGGGTGATACTGAAAAGCGGGCTACAAAGGGAAAGATGGACAGAGTTTCAGATGGACTGGATGCAAATGGTACGCAATCATCGAATTCTGTAACAGATGCGCAGAGAAGGTGGAGGCGTCTGAAGAACACAGTGACTGTTGTTAACCGTATGACTTCTA
GCAAGTCTTCAAATTATGATCTTTTGGAAGAAGAAGCTGTTGAAGGCATTCAAAAGGGCTCATTATATGCAAAAGGAGATGCAGGAAGCATGGGAGCAGCAGCATTTGAAGGAGTGAGGACCACAGCACGAAGCAGACGGAAAGCTCCCTATTACTCACTTCTTGATTGGGGTCTTCCTTGTTCCACTTGCACTTGGGTT
ACTTTTCACACCGTTATATTTGTTGGATATTAATGGACTGAGTTTTGATGGAGTAATGGATGAAAATTTGAATGACAGTCCAATGGCATCTACCAAACGTCGGTGTTTTGCTCTTTTGAACGTGTTTCTTTATGCATTGTCACTTTCAACAACATTCGGTGGGTCTCCAGTTGCCGCAGTGTCTCCTTTTTGCCTCCTTG
TGGCAAACATGAACACACTAATGGCACAGTTTCTGTTTGTCTTTCTTAGTGGAGCTGTTTTTGCACGCATGTCACAACCATCACAGCCGATTCGATGTGCTAAGAAAGCGATAATCAAGACCGATGATGTTACTATAAATCCTGGAGAGTATTCTGAAGAAAAAATCAAGGTGTTTGCTGTAAGGTTGGTGCTTGGAGGG
CCAACACCTTGTGAGCTTGTTGATGCAAAGATATGCTTGACATTCAGGATTTTCGTCACGTTATCAAATGGTTCCACATTTTGCTCCACTCAAGATTTGGACATTGTCCGATCAGAGGTTTCATACCTTCGATATGGACTTATGGTACGGCATGTTATTGACAAAAAGAGCCCTGTATATGGGCATACACTAGAAAGTTT
GGATGAAGGAGATGCCTCATTTTCATTAACAATCATGGGGATGGAAAGGTCATCAATGCAATCTATATTTCACCTGGAGGATTATTTTGTCTCTGATGGTGATGTTGTATGGGATGGAGATTATGTAGATTTCATATATATAAATCAAAAGGGTCAGCGTGTGCTTGATCATTCAAAGACTGACCTCCTAAAGCCGAAGA
AAGCTACTCGGGCAGATGGCGAATCTGGAGTAGAGAATGATTCTATCAAAAAGGAGCCAGAACAAGGAGGTAGTGTGGGGAAGTAGATAATGGTGCAGGAAGTATCAGCTTGAGAAACGTGTTATTGACTTATTGCATTTTCAGCCACAGGTTATTCAAGTCTATATTCTGTCGCGGGAATATTGGTTACTTCTTAGGCT
ACCTCATTCTTTAAATCCATTGCAGATGTTCTAAATCAGCCTTTACATAGCGTTATATTTGTTAACATGGACAGTTGGTACTAGTTTTTAATATGTATGTGAAGAGGCAATCCATCTATGGCAACATTAAGGCTCAAGTTTTATTTGTTTCATCCTCAGCTGAATTGATTTGAAGCTGTCAATATTCTGATGTAAATCAG
TATTTTTCTTCTTTGCCTGTTTCATCATTCGCTTATTATAGTGTGTCGTGATTGGAAAAAAAGAAATAAATTGAAGCTTACATTTGTTGTACAGGTACTAATTTCTAAACCATGTCGTTTATGTCATTGTTGAAAATATCGAAGAAAAAAAACTGCATAGCGTCCAGACCTCCGGACATTGGAAACAGCAGTAAAAAATA
TTGGCG

>TRINITY_DN118749_c0_g1_i1 from sample A3 / Example unspliced isoform:

TGGACCCCTTTAGCAAGCCGCTGTTGCTTCTTCCACCAGCCTACATTCATCAGTCATTCATTCCCTTTAGCCTTTGCTTCTGATACTTGATCCTCGCTACAACAACAGCTATTGATTTTGATTTCCCTATCAAAATAAAAGGTCGCAGGTTCGAGTTCTCAGAAGCGGGAGCACAGACGTACATACAATCTTAAAGATAT
AGCTTGCTGATACTAAGGAAATATAGGGTTTACTACACAATCTTGTCAAAACGGCGTTGAGGCGAGAAAATCTGCGTTACAGGTTGACTTCAGCAAGGTCGATTGAAGAGACAGGACTAGATTCTTTCTCTTATGGGGACGGCTTCTCATATTATCCTGCTCGTGCAACAATTCCAGCCCCTCAAGAGGGCTTCAACACT
GCTCCAAAGCTTAATACAAAAACAATGACAAGTAGGGATGCATACCTTGCTAGACAAGGTGCCTATCGAGATGTCCATTTTGCACACCAGCAGAGCTTGGTTCCAGATTATGAGGAGAAACACAGCTTCATAGATGGTATCCCTGAAATGCAGGCTAGTGGGGAGAAGGATGATCCTTTTGAGAATGGACAAACTTGGAA
TGAGAAAAGGAGAGTGAGAAGAAGTAATAGCAAAAATGGAAGATTAGGCAATTTCAAGAAATTGCAAGAGAAGGAATTTTGCTTTGCAGATTCTGCTGTAAAGGATGAAAGAGGCTGCACCACAGTTTTACCTGTCGATGGAAAATATATTGAGAATAACAATTTCCTTCCATCAAACAATAGGAAGATCAAGGCAAAGC
GGCTTCTGACCAACTCACATATGCAGGATAAAAATACAGAGACAAAATTGGATAACCATTCAAATGAATCTGCTGAGAATGTCATGTTCACTAAACATGACTCTACTTCTGAGCAACAGGATGAAAGACAACAATGCAGTTTGACCACTGGGGAAGCAGATAGTGTTACATTTTCATTGGTCACTTCGAAACATAACAAT
ACTACTGAGGGTGCTAAACTAATGCATACCTCTATGGATCAGTTGTTAAATGATACCTCTCCAACTTGCTCCAAGACTGTGTTTGAACAATCTTATCTTATATCTAATGCAAAGCTTTCATCATCTGGCACTCATACAGACGATTGCATAGATGGTTTGCCAACACAAAAAAATGATGTATCTTCATGTGAGCCATCCCC
AGCCAGCAACCAGAATGAGATTGTTGCAGATTCCTGTTTACCGGGTGATACTGAAAAGCGGGCTACAAAGGGAAAGATGGACAGAGTTTCAGATGGACTGGATGCAAATGGTACGCAATCATCGAATTCTGTAACAGATGCGCAGAGAAGGTGGAGGCGTCTGAAGAACACAGTGACTGTTGTTAACCGTATGACTTCTA
GCAAGTCTTCAAATTATGATCTTTTGGAAGAAGAAGCTGTTGAAGGCATTCAAAAGGGCTCATTATATGCAAAAGGAGATGCAGGAAGCATGGGAGCAGCAGCATTTGAAGGAGTGAGGACCACAGCACGAAGCAGACGGAAAGgtagaaaagggatggaaggattacttgtgagacaggacaaaggattcattactttt
atccaagatatttatgtaacctgcttgaagCTCCCTATTACTCACTTCTTGATTGGGGTCTTCCTTGTTCCACTTGCACTTGGGTTACTTTTCACACCGTTATATTTGTTGGATATTAATGGACTGAGTTTTGATGGAGTAATGGATGAAAATTTGAATGACAGTCCAATGGCATCTACCAAACGTCGGTGTTTTGCTCT
TTTGAACGTGTTTCTTTATGCATTGTCACTTTCAACAACATTCGGTGGGTCTCCAGTTGCCGCAGTGTCTCCTTTTTGCCTCCTTGTGGCAAACATGAACACACTAATGGCACAGTTTCTGTTTGTCTTTCTTAGTGGAGCTGTTTTTGCACGCATGTCACAACCATCACAGCCGATTCGATGTGCTAAGAAAGCGATAA
TCAAGACCGATGATGTTACTATAAATCCTGGAGAGTATTCTGAAGAAAAAATCAAGGTGTTTGCTGTAAGGTTGGTGCTTGGAGGGCCAACACCTTGTGAGCTTGTTGATGCAAAGATATGCTTGACATTCAGGATTTTCGTCACGTTATCAAATGGTTCCACATTTTGCTCCACTCAAGATTTGGACATTGTCCGATCA
GAGGTGACTTTGCTTTAAAGTTATGATATAAATTTGTGATTTTATTTCTAGTTTATGAGATGCTCAAAAATTGAAGTTTCAGACTAGTGACTAGTGACC