sp|P19243|HSP11_PEA ----> 18.1 kDa class I heat shock protein

Intron 1 GTTAGCATCT ... GCGATTGTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	80.77	5	29	13
A2	84	2	7	14
A3	86.67	2	11	15
A4	72.5	11	14	44
A5	100	0	8	0
A6	73.91	27	57	96
A7	76.24	48	116	192
A8	79.12	26	80	117
A9	74.47	36	100	110
A10	72.23	69	139	220

>TRINITY_DN44434_c0_g2_i1 from sample A6 / Example spliced isoform

TATGGGTGTCCTTCATAAGGAAATGCCCTTTTAGGAAAATTTTCTTTAGTGCATGCTACATTTCTCCATCCTCATCACTATAGGCCCTTACAGAGATTGATGCCTCTCCTTTCACGCAATTGCACAATGAAATATCAAAATTCATGGCTATTGCCATAAATTGTTTTCTATTTTCAGGCGTTACGGCGTATACACCATTA
AAAAAACTTCTAGGCGTTCTTGAAACTTCTACCCTTGCGGAATAACTATAGTTCACGATTCCAGAAGTCATTTGACGGAAGCCTATAAATTAAAATCAAGAAGATCCTTGTTCTCGAAACTTCGAGTGTTGCTGGGCGTCTCTGGAACCATCATCGATATAAAGAATAAACTTGAAAGAATGGCTTTAACTCCTTTCTTC
GGTCGATCGAGCGCTGGAAGCCTGTGGGATCCATGGGACCGTAGCAGCAGTTTGTTCGATCCGTCGGTACCCGTTTCTCGAATCTGGGACATCTTCGATTTCGGCAGTGCGCCGGACTCTCCAGCCTTTTCCTTCTCAAGGGACGCGCAGGCCATCGCCAACACCAGACTCGACTGGAAAGAAACCCCGGAGGCTCACGT
TTTCACCGCGGATTTGCCCGGGTTGAAGAAGGAAGAATTGAAGGTCGAATTGGTAGACAAAGGCAGCCTGAGAATAAGCGGAGAAAGGCACAAAGAAGATGTGCAGGACACCGACCAGTGGCACCGGGTGGAACGGTCCAGCGGACGATTCATG

>TRINITY_DN44434_c0_g2_i3 from sample A6 / Example unspliced isoform:

TATGGGTGTCCTTCATAAGGAAATGCCCTTTTAGGAAAATTTTCTTTAGTGCATGCTACATTTCTCCATCCTCATCACTATAGGCCCTTACAGAGATTGATGCCTCTCCTTTCACGCAATTGCACAATGAAATATCAAAATTCATGGCTATTGCCATAAATTGTTTTCTATTTTCAGGCGTTACGGCGTATACACCATTA
AAAAAACTTCTAGGCGTTCTTGAAACTTCTACCCTTGCGGAATAACTATAGTTCACGATTCCAGAAGTCATTTGACGGAAGCCTATAAATTAAAATCAAGAAGATCCTTGTTCTCGAAACTTCGAGTGTTGCTGGGCGTCTCTGGAACCATCATCgttagcatctttaacactataaatttcccttcattctctgagtct
gcattcaacgattgtatccgcgaatttcctctacttcatacttgtttaatttgcaattgtacactgtaaattctctggatttgctcgagttgcgattgtagGATATAAAGAATAAACTTGAAAGAATGGCTTTAACTCCTTTCTTCGGTCGATCGAGCGCTGGAAGCCTGTGGGATCCATGGGACCGTAGCAGCAGTTTG
TTCGATCCGTCGGTACCCGTTTCTCGAATCTGGGACATCTTCGATTTCGGCAGTGCGCCGGACTCTCCAGCCTTTTCCTTCTCAAGGGACGCGCAGGCCATCGCCAACACCAGACTCGACTGGAAAGAAACCCCGGAGGCTCACGTTTTCACCGCGGATTTGCCCGGGTTGAAGAAGGAAGAATTGAAGGTCGAATTGGT
AGACAAAGGCAGCCTGAGAATAAGCGGAGAAAGGCACAAAGAAGATGTGCAGGACACCGACCAGTGGCACCGGGTGGAACGGTCCAGCGGACGATTCATG

Intron 2 GTAAGTTGCG ... ATGCGTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	1.77	56	1	0
A2	15.34	32	6	0
A3	8.33	45	4	2
A4	19.43	74.5	18	0
A5	3.13	28	1	0
A6	6.2	399	26	0
A7	4.78	744	37.5	2
A8	6.74	521.5	37.5	0
A9	5.71	418.5	25	0
A10	5.71	767	46.5	6

>TRINITY_DN45304_c1_g1_i3 from sample A10 / Example spliced isoform

GGCTACCCTTGGGGAATAACTATAGTTCACGATTCCAGAAGTCATTTGACGGAAGCCTATAAATTAAAATCAAGAAGATCCTTGTTCTCGAAACTTCGAGTGTTGCTGGGCGTCTCTGGAACCATCATCGTTGGCATCTTTAACACTATAAATTTCCCTTCATTCTCTGAGTCTGCATTCAACGATTGTATCCGCGAATT
TCTTCTACTTCATACTTGTTTAATTTGCAATTGTACACTGTAAATTCTCTGGATTTGCTCGAGTTGCGATTGTAGGATAAGAATAAACTTGAAAGAATGGCTTTAACTCCTTTCTTCGGTCGATCGAGCGCTGGCAGCCTGTGGGATCCATGGGACCGTAGCAACAGTGTGTTCGATCCCTGGTTACCCGTTTCTCGAAT
CTGGGACGCCTTCGACTTCGGCAGCGCGCTGGACAGCCCGGCCTTTTCCTTCTCAAGGGACGCGCAGGCCATCGCCAACACCAGACTCGACTGGAAAGAAACCCCGGAGGCTCACGTTTTCACCGCGGATTTGCCCGGGTTGAATAAGGAAGAATTAAAGCTCGAATTGGTAGACAAAGGCAGTCTGAGAATAAGCGGAG
AAAGGCACAAAGAAGATGTGCAGGATACCGACCAGTGGCACCGGGTGGAACGGTCCAGCGGACAATTCATGAGGCAATTCCGGCTGCCGGAGAATGTGAATACCGATGGCATTTCCGCGAAATTGGAGAACGGTGTTCTGACTGTGAAT

>TRINITY_DN45304_c1_g1_i7 from sample A10 / Example unspliced isoform:

GGCTACCCTTGGGGAATAACTATAGTTCACGATTCCAGAAGTCATTTGACGGAAGCCTATAAATTAAAATCAAGAAGATCCTTGTTCTCGAAACTTCGAGTGTTGCTGGGCGTCTCTGGAACCATCATCGTTGGCATCTTTAACACTATAAATTTCCCTTCATTCTCTGAGTCTGCATTCAACGATTGTATCCGCGAATT
TCTTCTACTTCATACTTGTTTAATTTGCAATTGTACACTGTAAATTCTCTGGATTTGCTCGAGTTGCGATTGTAGGATAAGAATAAACTTGAAAGAATGGCTTTAACTCCTTTCTTCGGTCGATCGAGCGCTGGCAGCCTGTGGGATCCATGGGACCGTAGCAACAGTGTGTTCGATCCCTGGTTACCCGTTTCTCGAAT
CTGGGACGCCTTCGACTTCGGCAGCGCGCTGGACAGCCCGGCCTTTTCCTTCTCAAGGGACGCGCAGGCCATCGCCAACACCAGACTCGACTGGAAAGAAACCCCGGAGGCTCACGTTTTCACCGCGGATTTGCCCGgtaagttgcgcttttctttgttttaattgccgtgcttttcgatttgcagtccatcctttaaat
aaccggagtctccgttgaatgtatgcgtgcagGGTTGAATAAGGAAGAATTAA