sp|F6H7K5|THI42_VITVI ----> Thiamine thiazole synthase 2, chloroplastic

Intron 1 GTACGTCTTC ... ATGCATGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	14.71	29	7	3
A2	15.66	35	11	2
A3	31.71	28	19	7
A4	20	20	7	3
A5	35.14	24	10	16
A6	19.34	640	197	110
A7	21.9	214	72	48
A8	16.51	182	46	26
A9	21.26	237	89	39
A10	16.51	134	39	14

>TRINITY_DN100041_c0_g1_i1 from sample A5 / Example spliced isoform

CTCAGGATTCCTACAGTTTTATCTTCAATTATTAACTCACAGAGCCTCAGGATTCCTATATATCGTCCATTGCCTTAACTTCGAGCTTATATATCGTCCATTGCCTTAACTTCGATCTTTGCCTTGCATCCATTCACTCATTCATTTGCTGAAGCAAGAAGAAAAGAAGAGGAATGGCGTCTGTTACAGCAGCCTCTGCC
TCTGCTCGCAGCTTGGTGGCGGGTTCGATACTCGAGGACCGCAAATTGATGTCCTCCAAGTTCGGTTCCCTGTCATCGTCGTTCTCATCCGTTCACGGCACCGCCTTCACGGTCTCTGCCCGCCCCTCGCCGGCAGCGGTACAAGCAACTCCTGCGATCACTTCTCTGTACTCCGACCGCAAATATGACCTGCAGAGCTT
CAAGTTCGAGCCCATCAGGGAGTCCATCGTTGCTCGGGAGATGACGCGGCGCTACATGATGGACATGATCACGCACGCCGATACGGACGTGGTGATCGTCGGTGCGGGTTCTGCAGGGCTCTCATGCGCCTACGAGCTCAGCAAGAACCCCGACGTCAAGGTTGCCATCGTAGAGCAGTCGGTGAGCCCCGGGGGCGGGG
CGTGGCTTGGGGGCCAGCTTTTCTCAGCCATGGTGGTGAGGAAGCCTGCGCACCTCTTCCTGGACGAGATCGGTGTCCCCTACGACGAGGCGGAGGACTACGTGGTCATCAAGCACGCAGCGTTGTTCACCTCGACTATCATGAGCAAGGTGCTCTCAAGGCCCAACGTGAAGCTCTTCAATGCTGTGGCTGCTGAGGAT
CTCATCATAAAGGAGGGCCGGGTGTCCGGCGTCGTGACCAACTGGGCTCTGGTGTCGATGAACCACAATACACAGTCGTGTATGGACCCCAACGTCATGGAGTCCAAGGTCGTGGTCAGCTCCTGCGGCCACGATGGCCCGTTCGGCGCCACTGGTGTCAAGAGGCTCAAGAGCGTCGGTATGATCGACAAGGTTCCCGG
CATGAAAGCCCTCGATATGAATACAGCCGAGGATGCCATTGTTCGACTCACCAGGGAGATTGTCCCCGGCATGATCGTTACTGGCATGGAGGTCGCCGAAATCGATGGCTCCCCTAGAATGGGGCCAACCTTCGGAGCGATGCTGGTTTCAGGGCAAAAGGCTGCACATCTGGCCCTCAAAGCCCTGGGGCAGCCCAATG
CATTGGATGGCACATTGAAGGATCATCCTGAACTCGTTTTGGCTTCAGCTGAGAATGAGATCACAACTGCATCTGCTTAGATGATCACCTCCTCCTATTCCTTTGAAGTCTATATGTATCTATTTATGTCATCTGAAACTTTTGGCTTACCCTGCTACATGCTAGATTTTGTATAGATGTTGTGAAGCTTTGCCATGGAG
TAGCGGATCTCTCTATCTTGCTGTAATAAAGTTGTTATAAAGTTGATGACGTTTTAATGTTACAAATATTCTCTGCCTTTTGCTGGGTTTTCTGCCTGGTTTTCTGTATTGGATGTTCTCCATTGTATTTCTTTTACAACATATTGTGTTTTTAAGCATAACAATAGCATTTAGATAAGTAATGTATGTAAGTTGAAGAT
GGCAATTTGAGGACAAGATGATTCTTATCTTGCTGTAATAAAGTTGTT

>TRINITY_DN100041_c0_g1_i2 from sample A5 / Example unspliced isoform:

CTCAGGATTCCTACAGTTTTATCTTCAATTATTAACTCACAGAGCCTCAGGATTCCTATATATCGTCCATTGCCTTAACTTCGAGCTTATATATCGTCCATTGCCTTAACTTCGATCTTTGCCTTGCATCCATTCACTCATTCATTTGCTGAAGCAAGAAGAAAAGAAGAGGAATGGCGTCTGTTACAGCAGCCTCTGCC
TCTGCTCGCAGCTTGGTGGCGGGTTCGATACTCGAGGACCGCAAATTGATGTCCTCCAAGTTCGGTTCCCTGTCATCGTCGTTCTCATCCGTTCACGGCACCGCCTTCACGGTCTCTGCCCGCCCCTCGCCGGCAGCGGTACAAGCAACTCCTGCGATCACTTCTCTGTACTCCGACCGCAAATATGACCTGCAGAGCTT
CAAGTTCGAGCCCATCAGGGAGTCCATCGTTGCTCGGGAGATGACGCGGCGCTACATGATGGACATGATCACGCACGCCGATACGGACGTGGTGATCGTCGGTGCGGGTTCTGCAGGGCTCTCATGCGCCTACGAGCTCAGCAAGAACCCCGACGTCAAGGTTGCCATCGTAGAGCAGTCGGTGAGCCCCGGGGGCGGGG
CGTGGCTTGGGGGCCAGCTTTTCTCAGCCATGGTGGTGAGGAAGCCTGCGCACCTCTTCCTGGACGAGATCGGTGTCCCCTACGACGAGGCGGAGGACTACGTGGTCATCAAGCACGCAGCGTTGTTCACCTCGACTATCATGAGCAAGGTGCTCTCAAGGCCCAACGTGAAGCTCTTCAATGCTGTGGCTGCTGAGGAT
CTCATCATAAAGGAGGGCCGGGTGTCCGGCGTCGTGACCAACTGGGCTCTGGTGTCGATGAACCACAATACACAGTCGTGTATGGACCCCAACGTCATGGAGTCCAAGGTCGTGGTCAGCTCCTGCGGCCACGATGGCCCGTTCGGCGCCACTGGTGTCAAGAGGCTCAAGAGCGTCGGTATGATCGACAAGGTTCCCGG
CATGAAAGCCCTCGATATGAATACAGCCGAGGATGCCATTGTTCGACTCACCAGGGAGATTGTCCCCGGCATGATCGTTACTGGCATGGAGGTCGCCGAAATCGATGGCTCCCCTAGAATGgtacgtcttcaagggtttttaatcctttgctctctttgtaggttttctttcatggaggtttggggtgttcagggttttg
atctttttttttttctttatgtttcattgtacttctgaaagatgagtgttgtgggtgtaatatgctaatttatggattgcatgcatgcagGGGCCAACCTTCGGAGCGATGCTGGTTTCAGGGCAAAAGGCTGCACATCTGGCCCTCAAAGCCCTGGGGCAGCCCAATGCATTGGATGGCACATTGAAGGATCATCCTGA
ACTCGTTTTGGCTTCAGCTGAGAATGAGATCACAACTTCATCTGCTTAGATTATCACCTCCTCCTACTCCTTTGAAGTCTATATGTATCTATTTATGTCATCTGAAACTTTTGGCTTACCCTGCTACATGCTAGATTTTGTATAGATGTTGTGAAGCTTTGCCATGGAGTAGCGGATCTCTCTATCTTGCTGTAATAAAG
TTGTTATAAAGTTGATGACGTTTTAATGTTACAAATATTCTCTGCCTTTTGCTGGGTTTTCTGCCTGGTTTTCTGTATTGGATGTTCTCCATTGTATTTCTTTTACAACATATTGTGTTTTTAAGCATAACAATAGCATTTAGATAAGTAATGTATGTAAGTTGAAGATGGCAATTTGAGGACAAGATGATTCTTATCTT
GCTGTAATAAAGTTGTT

Intron 2 GTACGTCTTC ... ATGCATGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	14.71	29	7	3
A2	15.66	35	11	2
A3	31.71	28	19	7
A4	20	20	7	3
A5	35.14	24	10	16
A6	19.34	640	197	110
A7	21.9	214	72	48
A8	16.51	182	46	26
A9	21.26	237	89	39
A10	16.51	134	39	14

>TRINITY_DN101651_c0_g1_i1 from sample A3 / Example spliced isoform

CGCGCAGAGCCTCAGGATTCCTACAGTTTTATCTTCAATTATTAACTCACAGAGCCTCAGGATTCCTATATATCGTCCATTGCCTTAACTTCGAGCTTATATATCGTCCATTGCCTTAACTTCGATCTTTGCCTTGCATCCATTCACTCATTCATTTGCTGAAGCAAGAAGAAAAGAAGAGGAATGGCGTCTGTTACAGC
AGCCTCTGCCTCTGCTCGCAGCTTGGTGGCGGGTTCGATACTCGAGGACCGCAAATTGATGTCCTCCAAGTTCGGTTCCCTGTCATCGTCGTTCTCATCCGTTCACGGCACCGCCTTCACGGTCTCTGCCCGCCCCTCGCCGGCAGCGGTACAAGCAACTCCTGCGATCACTTCTCTGTACTCCGACCGCAAATATGACC
TGCAGAGCTTCAAGTTCGAGCCCATCAGGGAGTCCATCGTTGCTCGGGAGATGACGCGGCGCTACATGATGGACATGATCACGCACGCCGATACGGACGTGGTGATCGTCGGTGCGGGTTCTGCAGGGCTCTCATGCGCCTACGAGCTCAGCAAGAACCCCGACGTCAAGGTTGCCATCGTAGAGCAGTCGGTGAGCCCC
GGGGGCGGGGCGTGGCTTGGGGGCCAGCTTTTCTCAGCCATGGTGGTGAGGAAGCCTGCGCACCTCTTCCTGGACGAGATCGGTGTCCCCTACGACGAGGCGGAGGACTACGTGGTCATCAAGCACGCAGCGTTGTTCACCTCGACTATCATGAGCAAGGTGCTCTCAAGGCCCAACGTGAAGCTCTTCAATGCTGTGGC
TGCTGAGGATCTCATCATAAAGGAGGGCCGGGTGTCCGGCGTCGTGACCAACTGGGCTCTGGTGTCGATGAACCACAATACACAGTCGTGTATGGACCCCAACGTCATGGAGTCCAAGGTCGTGGTCAGCTCCTGCGGCCACGATGGCCCGTTCGGCGCCACTGGTGTCAAGAGGCTCAAGAGCGTCGGTATGATCGACA
AGGTTCCCGGCATGAAAGCCCTCGATATGAATACAGCCGAGGATGCCATTGTTCGACTCACCAGGGAGATTGTCCCCGGCATGATCGTTACTGGCATGGAGGTCGCCGAAATCGATGGCTCCCCTAGAATGGGGCCAACCTTCGGAGCGATGCTGGTTTCAGGGCAAAAGGCTGCACATCTGGCCCTCAAAGCCCTGGGG
CAGCCCAATGCATTGGATGGCACATTGAAGGATCATCCTGAACTCGTTTTGGCTTCAGCTGAGAATGAGATCACAACTGCATCTGCTTAGATGATCACCTCCTCCTATTCCTTTGAAGTCTATATGTATCTATTTATGTCATCTGAAACTTTTGGCTTACCCTGCTACATGCTAGATTTTGTATAGATGTTGTGAAGCTT
TGCCATGGAGTAGCGGATCTCTCTATCTTGCTGTAATAAAGTTGTTATAAAGTTGATGACGTTTTAATGTTACAAATATTCTCTGCCTTTTGCTGGGTTTTCTGCCTGGTTTTCTGTATTGGATGTTCTCCATTGTATTTCTTTTACAACATATTGTGTTTTTAAGCATAACAATAGCATTTAGATAAGTAATGTATGTA
AGTTGAAGATGGCAATTTGAGGACAAGATGAT

>TRINITY_DN101651_c0_g1_i2 from sample A3 / Example unspliced isoform:

CGCGCAGAGCCTCAGGATTCCTACAGTTTTATCTTCAATTATTAACTCACAGAGCCTCAGGATTCCTATATATCGTCCATTGCCTTAACTTCGAGCTTATATATCGTCCATTGCCTTAACTTCGATCTTTGCCTTGCATCCATTCACTCATTCATTTGCTGAAGCAAGAAGAAAAGAAGAGGAATGGCGTCTGTTACAGC
AGCCTCTGCCTCTGCTCGCAGCTTGGTGGCGGGTTCGATACTCGAGGACCGCAAATTGATGTCCTCCAAGTTCGGTTCCCTGTCATCGTCGTTCTCATCCGTTCACGGCACCGCCTTCACGGTCTCTGCCCGCCCCTCGCCGGCAGCGGTACAAGCAACTCCTGCGATCACTTCTCTGTACTCCGACCGCAAATATGACC
TGCAGAGCTTCAAGTTCGAGCCCATCAGGGAGTCCATCGTTGCTCGGGAGATGACGCGGCGCTACATGATGGACATGATCACGCACGCCGATACGGACGTGGTGATCGTCGGTGCGGGTTCTGCAGGGCTCTCATGCGCCTACGAGCTCAGCAAGAACCCCGACGTCAAGGTTGCCATCGTAGAGCAGTCGGTGAGCCCC
GGGGGCGGGGCGTGGCTTGGGGGCCAGCTTTTCTCAGCCATGGTGGTGAGGAAGCCTGCGCACCTCTTCCTGGACGAGATCGGTGTCCCCTACGACGAGGCGGAGGACTACGTGGTCATCAAGCACGCAGCGTTGTTCACCTCGACTATCATGAGCAAGGTGCTCTCAAGGCCCAACGTGAAGCTCTTCAATGCTGTGGC
TGCTGAGGATCTCATCATAAAGGAGGGCCGGGTGTCCGGCGTCGTGACCAACTGGGCTCTGGTGTCGATGAACCACAATACACAGTCGTGTATGGACCCCAACGTCATGGAGTCCAAGGTCGTGGTCAGCTCCTGCGGCCACGATGGCCCGTTCGGCGCCACTGGTGTCAAGAGGCTCAAGAGCGTCGGTATGATCGACA
AGGTTCCCGGCATGAAAGCCCTCGATATGAATACAGCCGAGGATGCCATTGTTCGACTCACCAGGGAGATTGTCCCCGGCATGATCGTTACTGGCATGGAGGTCGCCGAAATCGATGGCTCCCCTAGAATGgtacgtcttcaagggtttttaatcctttgctctctttgtaggttttctttcatggaggtttggggtgtt
cagggttttgatcttttttttttctttatgtttcattgtacttctgaaagatgagtgttgtgggtgtaatatgctaatttatggattgcatgcatgcagGGGCCAACCTTCGGAGCGATGCTGGTTTCAGGGCAAAAGGCTGCACATCTGGCCCTCAAAGCCCTGGGGCAGCCCAATGCATTGGATGGCACATTGAAGGA
TCATCCTGAACTCGTTTTGGCTTCAGCTGAGAATGAGATCACAACTGCATCTGCTTAGATGATCACCTCCTCCTATTCCTTTGAAGTCTATATGTATCTATTTATGTCATCTGAAACTTTTGGCTTACCCTGCTACATGCTAGATTTTGTATAGATGTTGTGAAGCTTTGCCATGGAGTAGCGGATCTCTCTATCTTGCT
GTAATAAAGTTGTTATAAAGTTGATGACGTTTTAATGTTACAAATATTCTCTGCCTTTTGCTGGGTTTTCTGCCTGGTTTTCTGTATTGGATGTTCTCCATTGTATTTCTTTTACAACATATTGTGTTTTTAAGCATAACAATAGCATTTAGATAAGTAATGTATGTAAGTTGAAGATGGCAATTTGAGGACAAGATGAT