sp|Q9SXZ2|FT_ARATH ----> Protein FLOWERING LOCUS T

Intron 1 GTATGCTACA ... CCATCTACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	18.57	38.3	7	4
A2	12.41	34.7	1	0.7
A3	8.26	46	1	0.7
A4	11.45	23.7	2	2.3
A5	2.04	13	0	0.7
A6	9.69	102.3	3	8
A7	31.03	200.7	28	51.3
A8	20.39	131.3	16	28.7
A9	10.99	230.3	8	21.3
A10	10.85	242.3	6	19.7

>TRINITY_DN43630_c0_g2_i6 from sample A9 / Example spliced isoform

AACCAGACCTGGTCTTGATTGCTATGTCGATGAATCCGATCCCCACTTTTTAGAACCCCAACGTTATCGCAGATACGTAATGGTTATGACGGATCCTAACGCTCCAGGCGGCGAGTATTTACACTGGATATTCAGCAGAATCATTACTGATCAGGAACATGGTGGCATAGATAGTCGCTCCGTGGACCTGGTTTCCCATG
AACCTCCAAGAACGGCTGGCAGCATGATAGTTTTCACAATGTTCATAACTCCAGAATCTCAGGCCATAGCTGTGCCTGTTTCGAGGGAGAACTTCAGTACCAGAGAATTTGCTGGGATGAATGGCTTTGGACTGCCCGTGGGTGCTGCTTTTTTCTACGTCAAACGTAACAATGAGAAGATTTGTAATGGTGGCGATAAA
TCGCCCACATGCATACACAATGAGGGACCATTACATAGTAAGATTGCCTGTGGAATTCCAAGAACCATGCGAAACGGTAAGATAGCTTTCGAATTGTGCAAGCCGAAGAGATTTAAATCCATGGTTCTTCCCAGCTCGGTGGAGAAGCAGAAGGGGGCCGATGTTGAAAATGGCAGCACTGGAAAGCGTGATGAGAATCA
TTCCAGCACATGTAGCACTGCACAGTTGTCTTGCAAGAGCGAATATGATGATTCGCATTAGGTAGCCGAGGACAGCCATCTACGTTGGGTTTATGCAAATAGGGTACACCATTTTCTCCGTATACTAGTGTTCTTTAACTCTATATTGCCGTGTTCAATAAATAAATCCAATTCATGTACCTTCGCTTTAGCTTTTTCGT
TATGTAATACAATCCAAGATCTTTTCATCTTTGATCTACTGCTGGTTATCTATTATTGATCAGAAGAGTTTACCTG

>TRINITY_DN43630_c0_g2_i2 from sample A9 / Example unspliced isoform:

GTAGATACGATTGAGTGTCAAAATACGGATGTAAGGCAGAAACTCCTGGAATCGGGAGTTATCGGGCGCGTTCTCAACGATTTCATGCCACGTATGCTGTTATAGATAATCTACCCAGATAAACGAGTTTCTTTAGGTGATCAACTCTGGCTCGCTCAAGTGCAAACCAGACTTTTTAGAACCCCAGCGTTATCGCAGAT
ACGTAATGGTAAGCAGGTTTAATTCCTATTTCGTCATTACATTCTGCAATATTTCTTTGTACATATTTTAAAGTTGTTCACAAATCAACACAATCAGGTTATGACGGATCCTAACGCTCCAGGCGGCGAGTATTTACACTGgtatgctacaatcgtcctggactttgatcaaatttcgtaatagcatagcacgggaatcg
attcattttttaataaaagcatcggttgttaccatctacagGATATTCAGCAGAATCATTACTGATCAGGAAGATGGTGGCATAGGTATAATCTCTGTCTTTCTTTGCTTGACCGTGAAACCGATCGATGAAATGCCTAGTAATATTTACCGTAAAATTATCATTATCTCTATCGGTTTCTTTGTGTGGCAATACTAGAT
AGTCGCTCCGTGGACCTGGTTTCCCATGAACCTCCAAGAACGGCTGGCAGCATGATAGTTTTCACAATGTTCATAACTCCAGAATCTCAGGCCATAGCTGTGCCTGTTTCGAGGGAGAACTTCAGTACCAGAGAATTTGCTGGGATGAATGGCTTTGGACTGCCCGTGGGTGCTGCTTTTTTCTACGTCAAACGTAACAA
TGAGAAGATTTGTAATGGTGGCGATAAATCGCCCACATGCATACACAATGAGGGACCATTACATAGTAAGATTGCCTGTGGAATTCCAAGAACCATGCGAAACGGTAAGATAGCTTTCGAATTGTGCAAGCCGAAGAGATTTAAATCCATGGTTCTTCCCAGCTCGGTGGAGAAGCAGAAGGGGGCCGATGTTGAAAATG
GCAGCACTGGAAAGCGTGATGAGAATCATTCCAGCACATGTAGCACTGCACAGTTGTCTTGCAAGAGCGAATATGATGATTCGCATTAGGTAGCCGAGGACAGCCATCTACGTTGGGTTTATGCAAATAGGGTACACCATTTTCTCCGTATACTAGTGTTCTTTAACTCTATATTGCCGTGTTCAATAAATAAATCCAAT
TCATGTACCTTCGCTTTAGCTTTTTCGTTATGTAATACAATCCAAGATCTTTTCATCTTTGATCTACTGCTGGTTATCTATTATTGATCAGAAGAGTTTACCTG

Intron 2 GTCATTCACC ... GGTGGCATAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	53.85	6	10	4
A2	44.44	5	1	7
A3	72.73	3	5	11
A4	82.61	2	6	13
A5	46.15	7	4	8
A6	54.33	29	21	48
A7	53.75	37	16	70
A8	55	45	31	79
A9	60	46	56	82
A10	63.43	32	39	72

>TRINITY_DN43630_c0_g2_i8 from sample A9 / Example spliced isoform

GTAGATACGATTGAGTGTCAAAATACGGATGTAAGGCAGAAACTCCTGGAATCGGGAGTTATCGGGCGCGTTCTCAACGATTTCATGCCACGTATGCTGTTATAGATAATCTACCCAGATAAACGAGTTTCTTTAGGTGATCAACTCTGGCTCGCTCAAGTGCAAACTACACCCGGTCTAGATTGCTATGTCAATGAATC
CGATCCCGGCTTGTTCGAAAACGAACGTTCTCGCAGATACGTAACGGTTATGACGGATCCTAGCGCTCCAGACGGCGAGTATTTACACTGTACTAGATAGTCGCCGGGTGGACCTGGTTTCCCATGAACCTCCAAGAATGGCTGGCTGCATGATAGTTTTCACAATGTTCACGACTCTAGAATCTCAGGCCATAGCTGTG
CCTGTTTCGAGGGAGAACTTCAGTACCAGAGCTTTTGCTGATATGAATGGCTTTGGACTGCCTCTGGGTGCTACTTTTTTCTACATCAAACGTGACAATGAGAAGATTTGGAATGGCGGCCGCGATAAATCGCCCACATGCATACGCAATGAGGGACCATCACATAGTAAGATTGCCTGTGCTATTCCAAGAACCCTGCG
AAACGGTAAGATAGCTTTCGAATTGTGCAAGCCGAAGGGATTTAAGTCCATCGTTCTTCCCAGCTCGGTGGAGAAGCAGAAGGGGGCCGATGTTGAAAATGGCAGCACTGGAAAGCGTGATGAGAATCATTCCAGCACATGTAGCACTGCACAGTTGTCTTGCAAGAGCGAATATGATGATTCGCATTAGGTAGCCGAGG
ACAGCCATCTACGTTGGGTTTATGCAAATAGGGTACACCATTTTCTCCGTATACTAGTGTTCTTTAACTCTATATTGCCGTGTTCAATAAATAAATCCAATTCATGTACCTTCGCTTTAGCTTTTTCGTTATGTAATACAATCCAAGATCTTTTCATCTTTGATCTACTGCTGGTTATCTATTATTGATCAGAAGAGTTT
ACCTG

>TRINITY_DN43630_c0_g2_i1 from sample A9 / Example unspliced isoform:

GTAGATACGATTGAGTGTCAAAATACGGATGTAAGGCAGAAACTCCTGGAATCGGGAGTTATCGGGCGCGTTCTCAACGATTTCATGCCACGTATGCTGTTATAGATAATCTACCCAGATAAACGAGTTTCTTTAGGTGATCAACTCTGGCTCGCTCAAGTGCAAACTACACCCGGTCTAGATTGCTATGTCAATGAATC
CGATCCCGGCTTGTTCGAAAACGAACGTTCTCGCAGATACGTAACGGTTATGACGGATCCTAGCGCTCCAGACGGCGAGTATTTACACTGgtcattcaccagaatcattacttataatgaggaatatggtggcatagTACTAGATAGTCGCCGGGTGGACCTGGTTTCCCATGAACCTCCAAGAATGGCTGGCTGCATGA
TAGTTTTCACAATGTTCACGACTCTAGAATCTCAGGCCATAGCTGTGCCTGTTTCGAGGGAGAACTTCAGTACCAGAGCTTTTGCTGATATGAATGGCTTTGGACTGCCTCTGGGTGCTACTTTTTTCTACATCAAACGTGACAATGAGAAGATTTGGAATGGCGGCCGCGATAAATCGCCCACATGCATACGCAATGAG
GGACCATCACATAGTAAGATTGCCTGTGCTATTCCAAGAACCCTGCGAAACGGTAAGATAGCTTTCGAATTGTGCAAGCCGAAGGGATTTAAGTCCATCGTTCTTCCCAGCTCGGTGGAGAAGCAGAAGGGGGCCGATGTTGAAAATGGCAGCACTGGAAAGCGTGATGAGAATCATTCCAGCACATGTAGCACTGCACA
GTTGTCTTGCAAGAGCGAATATGATGATTCGCATTAGGTAGCCGAGGACAGCCATCTACGTTGGGTTTATGCAAATAGGGTACACCATTTTCTCCGTATACTAGTGTTCTTTAACTCTATATTGCCGTGTTCAATAAATAAATCCAATTCATGTACCTTCGCTTTAGCTTTTTCGTTATGTAATACAATCCAAGATCTTT
TCATCTTTGATCTACTGCTGGTTATCTATTATTGATCAGAAGAGTTTACCTG

Intron 3 GTAAGCAGGT ... ACACAATCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	10.14	49.5	4	7
A2	2.12	46.5	1	1
A3	1.91	53.5	1	1
A4	14.61	26.5	4	5
A5	6.63	14.5	1	1
A6	1.36	114.5	1	2
A7	4.69	212	3	17
A8	10.25	132	9	19
A9	1.21	249.5	1	5
A10	1.6	257	1	7

>TRINITY_DN43630_c0_g2_i6 from sample A9 / Example spliced isoform

AACCAGACCTGGTCTTGATTGCTATGTCGATGAATCCGATCCCCACTTTTTAGAACCCCAACGTTATCGCAGATACGTAATGGTTATGACGGATCCTAACGCTCCAGGCGGCGAGTATTTACACTGGATATTCAGCAGAATCATTACTGATCAGGAACATGGTGGCATAGATAGTCGCTCCGTGGACCTGGTTTCCCATG
AACCTCCAAGAACGGCTGGCAGCATGATAGTTTTCACAATGTTCATAACTCCAGAATCTCAGGCCATAGCTGTGCCTGTTTCGAGGGAGAACTTCAGTACCAGAGAATTTGCTGGGATGAATGGCTTTGGACTGCCCGTGGGTGCTGCTTTTTTCTACGTCAAACGTAACAATGAGAAGATTTGTAATGGTGGCGATAAA
TCGCCCACATGCATACACAATGAGGGACCATTACATAGTAAGATTGCCTGTGGAATTCCAAGAACCATGCGAAACGGTAAGATAGCTTTCGAATTGTGCAAGCCGAAGAGATTTAAATCCATGGTTCTTCCCAGCTCGGTGGAGAAGCAGAAGGGGGCCGATGTTGAAAATGGCAGCACTGGAAAGCGTGATGAGAATCA
TTCCAGCACATGTAGCACTGCACAGTTGTCTTGCAAGAGCGAATATGATGATTCGCATTAGGTAGCCGAGGACAGCCATCTACGTTGGGTTTATGCAAATAGGGTACACCATTTTCTCCGTATACTAGTGTTCTTTAACTCTATATTGCCGTGTTCAATAAATAAATCCAATTCATGTACCTTCGCTTTAGCTTTTTCGT
TATGTAATACAATCCAAGATCTTTTCATCTTTGATCTACTGCTGGTTATCTATTATTGATCAGAAGAGTTTACCTG

>TRINITY_DN43630_c0_g2_i2 from sample A9 / Example unspliced isoform:

GTAGATACGATTGAGTGTCAAAATACGGATGTAAGGCAGAAACTCCTGGAATCGGGAGTTATCGGGCGCGTTCTCAACGATTTCATGCCACGTATGCTGTTATAGATAATCTACCCAGATAAACGAGTTTCTTTAGGTGATCAACTCTGGCTCGCTCAAGTGCAAACCAGACTTTTTAGAACCCCAGCGTTATCGCAGAT
ACGTAATGgtaagcaggtttaattcctatttcgtcattacattctgcaatatttctttgtacatattttaaagttgttcacaaatcaacacaatcagGTTATGACGGATCCTAACGCTCCAGGCGGCGAGTATTTACACTGGTATGCTACAATCGTCCTGGACTTTGATCAAATTTCGTAATAGCATAGCACGGGAATCG
ATTCATTTTTTAATAAAAGCATCGGTTGTTACCATCTACAGGATATTCAGCAGAATCATTACTGATCAGGAAGATGGTGGCATAGGTATAATCTCTGTCTTTCTTTGCTTGACCGTGAAACCGATCGATGAAATGCCTAGTAATATTTACCGTAAAATTATCATTATCTCTATCGGTTTCTTTGTGTGGCAATACTAGAT
AGTCGCTCCGTGGACCTGGTTTCCCATGAACCTCCAAGAACGGCTGGCAGCATGATAGTTTTCACAATGTTCATAACTCCAGAATCTCAGGCCATAGCTGTGCCTGTTTCGAGGGAGAACTTCAGTACCAGAGAATTTGCTGGGATGAATGGCTTTGGACTGCCCGTGGGTGCTGCTTTTTTCTACGTCAAACGTAACAA
TGAGAAGATTTGTAATGGTGGCGATAAATCGCCCACATGCATACACAATGAGGGACCATTACATAGTAAGATTGCCTGTGGAATTCCAAGAACCATGCGAAACGGTAAGATAGCTTTCGAATTGTGCAAGCCGAAGAGATTTAAATCCATGGTTCTTCCCAGCTCGGTGGAGAAGCAGAAGGGGGCCGATGTTGAAAATG
GCAGCACTGGAAAGCGTGATGAGAATCATTCCAGCACATGTAGCACTGCACAGTTGTCTTGCAAGAGCGAATATGATGATTCGCATTAGGTAGCCGAGGACAGCCATCTACGTTGGGTTTATGCAAATAGGGTACACCATTTTCTCCGTATACTAGTGTTCTTTAACTCTATATTGCCGTGTTCAATAAATAAATCCAAT
TCATGTACCTTCGCTTTAGCTTTTTCGTTATGTAATACAATCCAAGATCTTTTCATCTTTGATCTACTGCTGGTTATCTATTATTGATCAGAAGAGTTTACCTG

Intron 4 GTATAATCTC ... GCAATACTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	16.36	46	10	8
A2	19.59	39	8	11
A3	15.34	69	12	13
A4	27.84	35	14	13
A5	25.37	25	2	15
A6	21.15	220	47	71
A7	24.79	443	117	175
A8	22.36	316	56	126
A9	24.18	453	123	166
A10	21.85	474	123	142

>TRINITY_DN43630_c0_g2_i5 from sample A9 / Example spliced isoform

AACCAGACCTGGTCTTGATTGCTATGTCGATGAATCCGATCCCCACTTTTTAGAACCCCAACGTTATCGCAGATACGTAATGGTTATGACGGATCCTAACGCTCCAGGCGGCGAGTATTTACACTGGATATTCAGCAGAATCATTACTGATCAGGAACATGGTGGCATAGATAGTCGCTCCGTGGACCTGGTTTCCCATG
AACCTCCAAGAACGGCTGGCAGCATGATAGTTTTCACAATGTTCATAACTCCAGAATCTCAGGCCATAGCTGTGCCTGTTTCGAGGGAGAACTTCAGTACCAGAGAATTTGCTGGGATGAATGGCTTTGGACTGCCCGTGGGTGCTGCTTTTTTCTACGTCAAACGTAACAATGAGAAGATTTGTAATGGTGGCGATAAA
TCGCCCACATGCATACACAATGAGGGACCATTACATAGTAAGATTGCCTGTGGAATTCCAAGAACCATGCGAAACCGTAAGATAGCTTTCGAAGAGATTTAAATCCATGGTTCTTCCCATCTCAGTGGAGAAGCAGAAAGGGGACGATGCTGAAAATGGCAGCACTGGAGGCG

>TRINITY_DN43630_c0_g2_i11 from sample A9 / Example unspliced isoform:

AACCAGACCTGGTCTTGATTGCTATGTCGATGAATCCGATCCCCACTTTTTAGAACCCCAACGTTATCGCAGATACGTAATGGTTATGACGGATCCTAACGCTCCAGGCGGCGAGTATTTACACTGGATATTCAGCAGAATCATTACTGATCAGGAACATGGTGGCATAGgtataatctctgtctttctttgcttgaccg
tgaaaccgatcgatgaaatgcctagtaatatttaccgtaaaattatcattatctctatcggtttctttgtgtggcaatactagATAGTCGCTCCGTGGACCTGGTTTCCCATGAACCTCCAAGAACGGCTGGCAGCATGATAGTTTTCACAATGTTCATAACTCCAGAATCTCAGGCCATAGCTGTGCCTGTTTCGAGGG
AGAACTTCAGTACCAGAGAATTTGCTGGGATGAATGGCTTTGGACTGCCCGTGGGTGCTGCTTTTTTCTACGTCAAACGTAACAATGAGAAGATTTGTAATGGTGGCGATAAATCGCCCACATGCATACACAATGAGGGACCATTACATAGTAAGATTGCCTGTGGAATTCCAAGAACCATGCGAAACCGTAAGATAGCT
TTCGAAGAGATTTAAATCCATGGTTCTTCCCATCTCAGTGGAGAAGCAGAAAGGGGACGATGCTGAAAATGGCAGCACTGGAGGCG

Intron 5 GTATGCTACA ... GGTGGCATAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	14.29	6	7	1
A2	58.33	5	1	7
A3	76.92	3	1	10
A4	77.78	2	2	7
A5	50	7	0	7
A6	57.97	29	3	40
A7	66.67	37	28	74
A8	60.53	45	16	69
A9	59.65	46	8	68
A10	67.35	32	6	66

>TRINITY_DN43630_c0_g2_i8 from sample A9 / Example spliced isoform

GTAGATACGATTGAGTGTCAAAATACGGATGTAAGGCAGAAACTCCTGGAATCGGGAGTTATCGGGCGCGTTCTCAACGATTTCATGCCACGTATGCTGTTATAGATAATCTACCCAGATAAACGAGTTTCTTTAGGTGATCAACTCTGGCTCGCTCAAGTGCAAACTACACCCGGTCTAGATTGCTATGTCAATGAATC
CGATCCCGGCTTGTTCGAAAACGAACGTTCTCGCAGATACGTAACGGTTATGACGGATCCTAGCGCTCCAGACGGCGAGTATTTACACTGTACTAGATAGTCGCCGGGTGGACCTGGTTTCCCATGAACCTCCAAGAATGGCTGGCTGCATGATAGTTTTCACAATGTTCACGACTCTAGAATCTCAGGCCATAGCTGTG
CCTGTTTCGAGGGAGAACTTCAGTACCAGAGCTTTTGCTGATATGAATGGCTTTGGACTGCCTCTGGGTGCTACTTTTTTCTACATCAAACGTGACAATGAGAAGATTTGGAATGGCGGCCGCGATAAATCGCCCACATGCATACGCAATGAGGGACCATCACATAGTAAGATTGCCTGTGCTATTCCAAGAACCCTGCG
AAACGGTAAGATAGCTTTCGAATTGTGCAAGCCGAAGGGATTTAAGTCCATCGTTCTTCCCAGCTCGGTGGAGAAGCAGAAGGGGGCCGATGTTGAAAATGGCAGCACTGGAAAGCGTGATGAGAATCATTCCAGCACATGTAGCACTGCACAGTTGTCTTGCAAGAGCGAATATGATGATTCGCATTAGGTAGCCGAGG
ACAGCCATCTACGTTGGGTTTATGCAAATAGGGTACACCATTTTCTCCGTATACTAGTGTTCTTTAACTCTATATTGCCGTGTTCAATAAATAAATCCAATTCATGTACCTTCGCTTTAGCTTTTTCGTTATGTAATACAATCCAAGATCTTTTCATCTTTGATCTACTGCTGGTTATCTATTATTGATCAGAAGAGTTT
ACCTG

>TRINITY_DN43630_c0_g2_i3 from sample A9 / Example unspliced isoform:

GTAGATACGATTGAGTGTCAAAATACGGATGTAAGGCAGAAACTCCTGGAATCGGGAGTTATCGGGCGCGTTCTCAACGATTTCATGCCACGTATGCTGTTATAGATAATCTACCCAGATAAACGAGTTTCTTTAGGTGATCAACTCTGGCTCGCTCAAGTGCAAACCAGACTTTTTAGAACCCCAGCGTTATCGCAGAT
ACGTAATGGTAAGCAGGTTTAATTCCTATTTCGTCATTACATTCTGCAATATTTCTTTGTACATATTTTAAAGTTGTTCACAAATCAACACAATCAGGTTATGACGGATCCTAACGCTCCAGGCGGCGAGTATTTACACTGgtatgctacaatcgtcctggactttgatcaaatttcgtaatagcatagcacgggaatcg
attcattttttaataaaagcatcggttgttacgatatacaggtcattcaccagaatcattacttataatgaggaatatggtggcatagTACTAGATAGTCGCCGGGTGGACCTGGTTTCCCATGAACCTCCAAGAATGGCTGGCTGCATGATAGTTTTCACAATGTTCACGACTCTAGAATCTCAGGCCATAGCTGTGCC
TGTTTCGAGGGAGAACTTCAGTACCAGAGCTTTTGCTGATATGAATGGCTTTGGACTGCCTCTGGGTGCTACTTTTTTCTACATCAAACGTGACAATGAGAAGATTTGGAATGGCGGCCGCGATAAATCGCCCACATGCATACGCAATGAGGGACCATCACATAGTAAGATTGCCTGTGCTATTCCAAGAACCCTGCGAA
ACGGTAAGATAGCTTTCGAATTGTGCAAGCCGAAGGGATTTAAGTCCATCGTTCTTCCCAGCTCGGTGGAGAAGCAGAAGGGGGCCGATGTTGAAAATGGCAGCACTGGAAAGCGTGATGAGAATCATTCCAGCACATGTAGCACTGCACAGTTGTCTTGCAAGAGCGAATATGATGATTCGCATTAGGTAGCCGAGGAC
AGCCATCTACGTTGGGTTTATGCAAATAGGGTACACCATTTTCTCCGTATACTAGTGTTCTTTAACTCTATATTGCCGTGTTCAATAAATAAATCCAATTCATGTACCTTCGCTTTAGCTTTTTCGTTATGTAATACAATCCAAGATCTTTTCATCTTTGATCTACTGCTGGTTATCTATTATTGATCAGAAGAGTTTAC
CTG