sp|Q8LAR0|B2DXI_ARATH ----> Protein BIIDXI

Intron 1 GTCTGCTGGT ... TTTTGAGAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	58.06	13	13	23
A2	82.05	7	31	33
A3	78.79	7	22	30
A4	82.22	4	17	20
A5	82.61	6	26	31
A6	67.96	33	61	79
A7	75.55	28	79	94
A8	62.69	36	45	76
A9	86.21	14	85	90
A10	71.43	32	73	87

>TRINITY_DN45193_c2_g1_i2 from sample A6 / Example spliced isoform

ATCTTCGTTACAGCAGAAGACGGCGGGGAGCGAAAATCGAACGTCATGGCGAGGCGTGAATGGCGTAGATTCGTTGCCCGGGTGGAGTATAGAAGGCACAGTTGAATACATAAACGAAGGGCAAAGGCAGGGCAGCATGTACCTGGTCGTTCCTGAAGGAAAGCATGCCGTAAGGCTGGGCAATGAGGCCCAGATTAGCC
AGCCAATAAGTCTTCAGAAAGGATCCACATATTCTCTGACATTTAGCGCGGCTCGAACCTGCGCGCAGTTGGAATCGCTTAACGTCACTGTGCCTCCGGCGACTAGGAATGTGGATTTGCAAACTTTGTATAGTATCTTTGGCTGGGATGCCTATGCTTGGGCTTTTCAGGCTCAATCTGAAAATGTCGAGGTTATTTTC
CAGAATCCGGGTTTGGAAGAAGATCCTACTTGCGGCCCTATTCTTGACGCCATTGCCTTGAAACAGATTTCTACACCTGAAAAACTCAAAGATAATGCTGTGGTTAATGGAGATTTCGAAGAGGGACCGTGGATGTTTGACAATGAGACGCTGGGCGTTCTTCTGCCGACAAATCTGGACGTTACAACATCTTCTTTGCC
CGGATGGATCGTGGAGTCGAGCAAAGCGGTGAGATATATCGACTCTACTCACTACACAGTTCCCGAGGGGAAGAGAGCCATCGAGCTTCTTTCTGGCAAAGAAGGTATCATCTCGCAGATGGTGGACACAAACCCCGGGAGCAAATATACTATGAGTTTTCTATTGGGAGAGGCCGGCGATGGCTGCCAGGAGCCAATGG
CGGTTATGGCTTTTGCCGGAGATGAATCGGATAGTGTCCACTTCAATAATCCTCCGGCAAGCGGCAAATACGAGCCTAATAATGTAACATTCACGGCGAAGGCCGAACGGACCCGCATTGCATTCTACAGTTTATATTATAATACAAGGATCAGAGATAAGAGTTCTCTGTGCGGTCCTGTGATTGATCAAGTAAAAGTT
GTCGATTTGTCCAAGTCGGGCGCTGTTGCAGCCTTTTCTGGATTCTCTCTTTTCCTGCTACTTTTGGTGACCGTGTCAACAATGGCGTTCACACTGGGTAATGTGTAAACTAATGTACGAAAAGCTTGGTATTGGAATTATAGCAAATGCTAAAATATAGCGGAATGTAGTCTCTCTGTGTTATGGTTATTTAGCAGTTT
TGACGAAAGTGTGTTTGAATGGTAGAATTTCTGTAAAGTTGGAAGGAAAAGAAAAGCATTCACCGCGGAATTGATTGGTGATGCGAACTGTAACTATTGTGGAAATCAAGTCGTGATGATTTGCCAGTTGTAAAGGACTGGAGTTTGAATCAATTCAAAGCCATGTGAAATCAGTAGAGGACATTGATGTGTGTCAACAT
TGTTATTATGTTCTGTGGTAACCAGCTCAGCTCTTCTTTCAAAAGCAACAGGGTGGGACCATATTGGTATCTCATTTTATATTTTAAATTCCTAAAATCTACCCCTCCATTTCTTCAAAATCACAATTTCTTCGTGGTTTATGATTTGACATTACACAGCCTCATTTTATATTTTCAATTCCTAAAATCTACCCCTACAT
TTCTTCAAATCACAATTTCTTCGTGGTTT

>TRINITY_DN45193_c2_g1_i3 from sample A6 / Example unspliced isoform:

CGGGAGGCTCGGGAAGACAGTAAATGCTAGGGTTACACTACAGAGCACCTGCTCTCCTATAAAGGAACGAGCTCACCCCATTGGGAAACTGGAAATGCAGCCCTGCATTTGTGCCTTATGTTCAGTTTGTCAAATTAATGCTGAAATTTAGGGTTTACTAGACTGTGGACAGTGAGTTAAAGATTTCAAGAATCAGAAAA
ACTGATGGCTAAAAATTCAGTGCTTCTAGTCTTGTCTCATGTTTGTTTTCTTGAAGTCATAATATGCCTAAGGATTCTCTCGATCTTCGTTACAGCAGAAGACGgtctgctggtgaatggaaattttgagaagGCGGGGAGCGAAAATCGAACGTCATGGCGAGGCGTGAATGGCGTAGATTCGTTGCCCGGGTGGAGTA
TAGAAGGCACAGTTGAATACATAAACGAAGGGCAAAGGCAGGGCAGCATGTACCTGGTCGTTCCTGAAGGAAAGCATGCCGTAAGGCTGGGCAATGAGGCCCAGATTAGCCAGCCAATAAGTCTTCAGAAAGGATCCACATATTCTCTGACATTTAGCGCGGCTCGAACCTGCGCGCAGTTGGAATCGCTTAACGTCACT
GTGCCTCCGGCGACTAGGAATGTGGATTTGCAAACTTTGTATAGTATCTTTGGCTGGGATGCCTATGCTTGGGCTTTTCAGGCTCAATCTGAAAATGTCGAGGTTATTTTCCAGAATCCGGGTTTGGAAGAAGATCCTACTTGCGGCCCTATTCTTGACGCCATTGCCTTGAAACAGATTTCTACACCTGAAAAACTCAA
AGATAATGCTGTGGTTAATGGAGATTTCGAAGAGGGACCGTGGATGTTTGACAATGAGACGCTGGGCGTTCTTCTGCCGACAAATCTGGACGTTACAACATCTTCTTTGCCCGGATGGATCGTGGAGTCGAGCAAAGCGGTGAGATATATCGACTCTACTCACTACACAGTTCCCGAGGGGAAGAGAGCCATCGAGCTTC
TTTCTGGCAAAGAAGGTATCATCTCGCAGATGGTGGACACAAACCCCGGGAGCAAATATACTATGAGTTTTCTATTGGGAGAGGCCGGCGATGGCTGCCAGGAGCCAATGGCGGTTATGGCTTTTGCCGGAGATGAATCGGATAGTGTCCACTTCAATAATCCTCCGGCAAGCGGCAAATACGAGCCTAATAATGTAACA
TTCACGGCGAAGGCCGAACGGACCCGCATTGCATTCTACAGTTTATATTATAATACAAGGATCAGAGATAAGAGTTCTCTGTGCGGTCCTGTGATTGATCAAGTAAAAGTTGTCGATTTGTCCAAGTCGGGCGCTGTTGCAGCCTTTTCTGGATTCTCTCTTTTCCTGCTACTTTTGGTGACCGTGTCAACAATGGCGTT
CACACTGGGTAATGTGTAAACTAATGTACGAAAAGCTTGGTATTGGAATTATAGCAAATGCTAAAATATAGCGGAATGTAGTCTCTCTGTGTTATGGTTATTTAGCAGTTTTGACGAAAGTGTGTTTGAATGGTAGAATTTCTGTAAAGTTGGAAGGAAAAGAAAAGCATTCACCGCGGAATTGATTGGTGATGCGAACT
GTAACTATTGTGGAAATCAAGTCGTGATGATTTGCCAGTTGTAAAGGACTGGAGTTTGAATCAATTCAAAGCCATGTGAAATCAGTAGAGGACATTGATGTGTGTCAACATTGTTATTATGTTCTGTGGTAACCAGCTCAGCTCTTCTTTCAAAAGCAACAGGGTGGGACCATATTGGTATCTCATTTTATATTTTAAAT
TCCTAAAATCTACCCCTCCATTTCTTCAAAATCACAATTTCTTCGTGGTTTATGATTTGACATTACACAGCCTCATTTTATATTTTCAATTCCTAAAATCTACCCCTACATTTCTTCAAATCACAATTTCTTCGTGGTTT

Intron 2 GTAAGAATAT ... TGTATATCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	23.53	13	7	1
A2	15.07	31	4	7
A3	27.87	22	7	10
A4	17.07	17	3	4
A5	17.46	26	3	8
A6	20.78	61	13	19
A7	17.71	79	25	9
A8	19.64	45	9	13
A9	16.67	85	12	22
A10	20.65	73	18	20

>TRINITY_DN95875_c0_g1_i4 from sample A4 / Example spliced isoform

GCCTTATGTTCAGTTTGTCAAATTAATGCTGAAATTTAGGGTTTACTAGACTGTGGACAGTGAGTTAAAGATTTCAAGAATCAGAAAAACTGATGGCTAAAAATTCAGTGCTTCTAGTCTTGTCTCATGTTTGTTTTCTTGAAGTCATAATATGCCTAAGGATTCTCTCGATCTTCGTTACAGCAGAAGACGGTCTGCTG
GTGAATGGAAATTTTGAGAAGGCGGGGAGCGAAAATCGAACGTCATGGCGAGGCGTGAATGGCGTAGATTCGTTGCCCGGGTGGAGTATAGAAGGCACAGTTGAATACATAAACGAAGGGCAAAGGCAGGGCAGCATGTACCTGGTCGTTCCTGAAGGAAAGCATGCCGTAAGGCTGGGCAATGAGGCCCAGATTAGCCA
GCCAATAAGTCTTCAGAAAGGATCCACATATTCTCTGACATTTAGCGCGGCTCGAACCTGCGCGCAGTTGGAATCGCTTAACGTCACTGTGCCTCCGGCGACTAGGAATGTGGATTTGCAAACTTTGTATAGTATCTTTGGCTGGGATGCCTATGCTTGGGCTTTTCAGGCTCAATCTGAAAATGTCGAGGTTATTTTCC
AGAATCCGGGTTTGGAAGAAGATCCTACTTGCGGCCCTATTCTTGACGCCATTGCCTTGAAACAGATTTCTACACCTGAAAAACTCAAAGGTAAGTTCAATATCGAACTCTTAAATGTGTCTTCATTTCTTGAAAATTAAATGTAG

>TRINITY_DN95875_c0_g1_i1 from sample A4 / Example unspliced isoform:

GCCTTATGTTCAGTTTGTCAAATTAATGCTGAAATTTAGGGTTTACTAGACTGTGGACAGTGAGTTAAAGATTTCAAGAATCAGAAAAACTGATGGCTAAAAATTCAGTGCTTCTAGTCTTGTCTCATGTTTGTTTTCTTGAAGTCATAATATGCCTAAGGATTCTCTCGATCTTCGTTACAGCAGAAGACGgtaagaat
atttaatgtgttcattttaatcttaaacttatgcttaatatatgctgtctgaatttatggaataaatgtgtatatcagGTCTGCTGGTGAATGGAAATTTTGAGAAGGCGGGGAGCGAAAATCGAACGTCATGGCGAGGCGTGAATGGCGTAGATTCGTTGCCCGGGTGGAGTATAGAAGGCACAGTTGAATACATAAAC
GAAGGGCAAAGGCAGGGCAGCATGTACCTGGTCGTTCCTGAAGGAAAGCATGCCGTAAGGCTGGGCAATGAGGCCCAGATTAGCCAGCCAATAAGTCTTCAGAAAGGATCCACATATTCTCTGACATTTAGCGCGGCTCGAACCTGCGCGCAGTTGGAATCGCTTAACGTCACTGTGCCTCCGGCGACTAGGAATGTGGA
TTTGCAAACTTTGTATAGTATCTTTGGCTGGGATGCCTATGCTTGGGCTTTTCAGGCTCAATCTGAAAATGTCGAGGTTATTTTCCAGAATCCGGGTTTGGAAGAAGATCCTACTTGCGGCCCTATTCTTGACGCCATTGCCTTGAAACAGATTTCTACACCTGAAAAACTCAAAGATAATGCTGTGGTTAATGGAGATT
TCGAAGAGGGACCGTGGATGTTTGACAATGAGACGCTGGGCGTTCTTCTGCCGACAAATCTGGACGTTACAACATCTTCTTTGCCCGGATGGATCGTGGAGTCGAGCAAAGCGGTGAGATATATCGACTCTACTCACTACACAGTTCCCGAGGGGAAGAGAGCCATCGAGCTTCTTTCTGGCAAAGAAGGTATCATCTCG
CAGATGGTGGACACAAACCCCGGGAGCAAATATACTATGAGTTTTCTATTGGGAGAGGCCGGCGATGGCTGCCAGGAGCCAATGGCGGTTATGGCTTTTGCCGGAGATGAATCGGATAGTGTCCACTTCAATAATCCTCCGGCAAGCGGCAAATACGAGCCTAATAATGTAACATTCACGGCGAAGGCCGAACGGACCCG
CATTGCATTCTACAGTTTATATTATAATACAAGGATCAGAGATAAGAGTTCTCTGTGCGGTCCTGTGATTGATCAAGTAAAAGTTGTCGATTTGTCCAAGTCGGGCGCTGTTGCAGCCTTTTCTGGATTCTCTCTTTTCCTGCTACTTTTGGTGACCGTGTCAACAATGGCGTTCACACTGGGTAATGTGTAAACTAATG
TACGAAAAGCTTGGTATTGGAATTATAGCAAATGCTAAAATATAGCGGAATGTAGTCTCTCTGTGTTATGGTTATTTAGCAGTTTTGACGAAAGTGTGTTTGAATGGTAGAATTTCTGTAAAGTTGGAAGGAAAAGAAAAGCATTCACCGCGGAATTGATTGGTGATGCGAACTGTAACTATTGTGGAAATCAAGTCGTG
ATGATTTGCCAGTTGTAAAGGACTGGAGTTTGAATCAATTCAAAGCCATGTGAAATCAGTAGAGGAC