NA ----> NA

Intron 1 GTATGTTAAT ... GGATGAGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	20	234	74	43
A2	17.2	195	46	35
A3	20.49	194	40	60
A4	17.83	159	38	31
A5	17.65	91	24	15
A6	15.47	1180	216	216
A7	17.58	1308	317	241
A8	18.01	1892	437	394
A9	17.1	1343	272	282
A10	16.05	931	228	128

>TRINITY_DN38320_c0_g1_i1 from sample A7 / Example spliced isoform

CATATTTCCTTTATTTCAAACCGTTCATTTCATTGTCCTTCTCGCCGATCGAGGCATAACGGACACAATCGTTTGACGAAATCTGAACCGTCGATGTAATGACCGTTACGGCCGTCCTCTGGGACGTTAAATGCTTTAAAATTTGAAATCGAGGGCGGTTTCCTCTCATCATCTTTTGCTTCGCGAGTTTTTGCCGTAGA
AATCGCATTCTCAGAGCGATAGAACAGAATACAAGTCCATTTTCATGCGAGAAACCCGCTTCTGTTGAAGGGAAAGAGCGATTCAGAGCCTGATTATGGCATCTCCATTTAGATCTCCTTCTCCTGCTCTGGACAGGCCCAAGTCTGCCTTAACAAGCCCAGGGAACGGTAATGGCCTACGGAAGAGCTTCAATGTCAAT
GTGCATCCCAAGGCTCCTGCGAAGAATCTCTCTGCCGTCACTCCGGCCAACAGCCCTGCAGGTAAACATTACAGAAAAATCTTGCAATGGCTTCTGTTGGATTAGGCGATGGTTTGGTATTTGTATTTATCTTGACTTGTGTTGCTTTCCATTGCAGACTATCCTCAGAGAGCACCATCTCCGTTCAGAGATTCAAATTT
CAGACAATCGGAGGAGATCAAAGAGAACAGCGGGATTGAGAATATCCGGCCTCATAAGTCTGCAAGCCTGAGGTCACCCACACCCAAAGGCATGAAGAGCTTCATGGCCCCAACATTTTCAGCTGCTTCCAAGGCTGCTGAACCCAGAAAGAGGGTTTTAATGGAGAAAAATGAGACTGTTGGGCATTCTGCTATTTCAA
TGCAATCACCGGCCAAGACCCTGGTGAAATCGCCGTCTGTGGCGAGGACCCAAATTTCGGCCCTGCATGAGCCTATGATGGAAGATAGCCCGCTCGCTAGAGAAGATCATGAAGAAGTTTCAGTGGATTGTATGGAGGTGGACGAGATCCAAACCACGCCGCAGCCTAAAAGCAAGAACTGCAAGGTACTTTTTGAAGCT
GGGGTTGTTCCCAATCCCATTGAGGGAAAGGAACCTAACCTTGAATCAAACGAGGATAGTGAGTCTTTAACCTTTCTGGCTCAGTTTCCTGATCCTGGCGACGAGCCTATAATCCTTCCGCCCTATGATCCTAAGACTAATTATCTGTCTCCCCGGCCTCAATTTCTTCGTTATCGTCCAGAAAGAGACCTAGTATTGGA
TGTTGGGGAGCAAGGTTTTGGGGGTCATGGTGTTTTTGAATATGGACCCAAGAGCGGGCTTACCGAAGTCGCAGAGATTGGGGAAATACAGTCCCTGGGAGAGCAAATGGCTTCTGTAGGCATATCCACCGATTGTGGAGAAATCCATGAAGAAATTCAAGATGTGGAAGAAATAGAAATAGCATCAGTTGATAAATCTG
AAGAAAAGCCCTGCAACACTGGTGGTGCCGCCTTTGAACCCAGGTTGAATACCTTTGAAAAAGAGAGGGCAATGTATGATGATGAAGACGAGGATGATGAGGAGGAGAAGCCCTCTGGATGGTTGAAAATGTCATTGCTGTTTGTATTTGTTTTGCTGTCTGCTTCAGTTGCTTTGTTGGGGTCGGGCAGCCCTGGTCTT
TTGCCACCTTCTCTGCAAAACAATGCATTGGCTACTGGTAAGTTTTATGACTCTGCAGTAAATGCTGTTAAAGATGTAGAAGGGTTTTGGAGAAAGGGTTCTGTCAAAGATATGAATCATTCTCTTTTAGAGTATTCAAGGACAAAATTTGATGTCACTATAGAGAATGTCAAGTTCTGGTTTGATTCATCCTTGACCAA
GCTAAAGATGCTAACAAATTCTGTCATGGAGAACTCCTTTGAGAAAACCCATAGTAGGAGTATGGAAGAAATGAGTAATCATCTTGGCTTGGAAACTATTGTAGAGAGGGACATCAAGGACCATAGCCTTCCCTCCGATGAGGGGTTACTGGAAGAGAAGGAGAGTAGCGCATCGGAGGGGTTACTGGAAGAGAAGGAGA
GTAGCACAGAGGCTGCTCAGGAGGGATTTGATCGGTTTCTTGTCTCTTCAGTTTATAATGAGCAGTCCACCCAAATAGCCAAAGGATTTGAGGGAGACCGTGTTCTAGAAGGGAATACTAAGCTTGAAGGTGATCTTCAGAGTGCATCTGAGAACTCATCATCCTTTGGGTTGCAAGAAGAAGAGCTTCAGAGTGCATCT
GAGAACCCATCATCCTTTGGGTTGCAAGAAGAAGAGAATAAAGTTGGAACACATGAACTTGTAGATTTCTACTCTGATATAAATGAAGAAAATGATATAGATGGTAGAGAAGAGGGATTTGAGGAATCTATATCCTCATCTCCTTCATATATAATAGACATCTCTGCTAGTTTTGAGGAATCTCCTCCCATGGCCCATTC
TGAAGAAGCAGATAATATCGATGAACATGCTTCTGAAGCAGTACACATTCCTGGGATTCCCATGGCCCCTTCTGAAGAAGCAGATAATATTGATGAACATGCTTCTGAAGCAGTACACATTCCTGGGATTCCTGAAACTACTGCCCTTCCTAACCCTGTCCAGATGTTTGCATCGGTTATGAGTGCAGATTCAGAAGTCA
GTGAACTTTTTGTTCCTTTGGTTTCACAGAGCAACACAGAAGTCAAGGAAGAGCCTGAGGCATTGAGCATAGAGAGCCAAACACACGAGGATGTGACTGTTGAAGATGGAAATTACAATGCTTCTCATTCTAGCATAGATGATGAGTACGTTCTGGAGGAGTTTAAATCCACAGAAAGGACTGAATCTACGATTTCCCAA
ATCAAAGAGAGTGCAGTAGAAGAATCTTATCCACATTCTGCAAAGGATTTGAATGGTGCTGGAGTTGATACTGCCACCCTTCGTAAGAGCTCAGTAAGAGAGCTTCTGCCGAGTGCAACTATAGGTGGTTTGTCAATTCTAGCAGCTACTGTATTAGCTGCCATTTATTTTGTTTCTGTCTCTAGGAAGAATTCTAAAAT
CTCTAGTGAAGTTGGAGGAAAATCAAACAGAGGGAGGTCAGTCAAAGTAACAAAAGAAGCAGGAAACATTTACTCCCCATACTCTAGGGTGCAGTTTATTTCTCCCACGCAAAACATGATGGAGAAGCGTGTAAATTCCAGGCTTTCTCCGCCTCATGTGGAATTACTTGCTGATTATTTTCCAGCAGAGATGAGTAGCT
CAGATAGAAATGTCAGTAGAGCGAGTACATATGACAATATTGCTCAGACATACGAAAGGAGGTCAAGAAGAGGAAACAGCATTTCCTCTGTAACTGAGCAAGAATATACAGTTACACCAGACTTTTCCACAGACTCACCTTCTTATGGAAGCTTTACAACTTATGAGAACATTGTCCGAAAAGAGGGTGGTCGAGATGAG
GTGCACAAGGTTATGAAGGTAGTCACTCCGGTGAGAAGATCTAGTAGAATCCGCAAGCAAGCAATATCTCCACAGTGATGTGATGATGAAAACAAAGTGGGTTTTTGATACCCCTGGCTCATCCTATCCGATGAGCAATATTTCTTTTATTGTTCTTGGGCATCATTTTATGTTTCAGAATTAAATTACTGCTTAAACAA
TGCATTCCTCATTGGAATGATGGCGACAAGGCAACACGATTACTTATGGGTTGTCCTAGTGCTCTGTAACTGAATGAATTAGTTAATTATAGATTTAACTGGTTTTTATTTTAGGGAAGAAATGTTTCCAGTGTTCATGCTCCAAATGAATTGGTTGCTTTTATGCTTCATGATCTTATATCTTTTTCGGTGTTTGGAGA
AGATTTTCACACATCCCGTTCAATGACAATGTTGTGTTTTCAATAAATTGCCCATGAGTTAGTTTGTTATCTACATCAGTAGAAGTAGATATGTTTGTGGAGTTGGCACTCTTAAATTGGTTTTCATGGCATTCTGTATTTATGCAAAAATAAAAAATAAAAACAAGCTCGTGGAGTTAGGTAC

>TRINITY_DN38320_c0_g1_i4 from sample A7 / Example unspliced isoform:

CATATTTCCTTTATTTCAAACCGTTCATTTCATTGTCCTTCTCGCCGATCGAGGCATAACGGACACAATCGTTTGACGAAATCTGAACCGTCGATGTAATGACCGTTACGGCCGTCCTCTGGGACGTTAAATGCTTTAAAATTTGAAATCGAGGGCGGTTTCCTCTCATCATCTTTTGCTTCGCGAGTTTTTGCCGTAGA
AATCGCATTCTCAGAGCGATAGAACAGAATACAAGTCCATTTTCATGCGAGAAACCCGCTTCTGTTGAAGGGAAAGAGCGATTCAGAGCCTGATTATGGCATCTCCATTTAGATCTCCTTCTCCTGCTCTGGACAGGCCCAAGTCTGCCTTAACAAGCCCAGGGAACGGTAATGGCCTACGGAAGAGCTTCAATGTCAAT
GTGCATCCCAAGGCTCCTGCGAAGAATCTCTCTGCCGTCACTCCGGCCAACAGCCCTGCAGACTATCCTCAGAGAGCACCATCTCCGTTCAGAGATTCAAATTTCAGACAATCGGAGGAGATCAAAGAGAACAGCGGGATTGAGAATATCCGGCCTCATAAGTCTGCAAGCCTGAGGTCACCCACACCCAAAGGCATGAA
GAGCTTCATGGCCCCAACATTTTCAGCTGCTTCCAAGGCTGCTGAACCCAGAAAGAGGGTTTTAATGGAGAAAAATGAGACTGTTGGGCATTCTGCTATTTCAATGCAATCACCGGCCAAGACCCTGGTGAAATCGCCGTCTGTGGCGAGGACCCAAATTTCGGCCCTGCATGAGCCTATGATGGAAGATAGCCCGCTCG
CTAGAGAAGATCATGAAGAAGTTTCAGTGGATTGTATGGAGGTGGACGAGATCCAAACCACGCCGCAGCCTAAAAGCAAGAACTGCAAGGTACTTTTTGAAGCTGGGGTTGTTCCCAATCCCATTGAGGGAAAGGAACCTAACCTTGAATCAAACGAGGATAGTGAGTCTTTAACCTTTCTGGCTCAGTTTCCTGATCCT
GGCGACGAGCCTATAATCCTTCCGCCCTATGATCCTAAGACTAATTATCTGTCTCCCCGGCCTCAATTTCTTCGTTATCGTCCAGAAAGAGACCTAGTATTGGATGTTGGGGAGCAAGGTTTTGGGGGTCATGGTGTTTTTGAATATGGACCCAAGAGCGGGCTTACCGAAGTCGCAGAGATTGGGGAAATACAGTCCCT
GGGAGAGCAAATGGCTTCTGTAGGCATATCCACCGATTGTGGAGAAATCCATGAAGAAATTCAAGATGTGGAAGAAATAGAAATAGCATCAGTTGATAAATCTGAAGAAAAGCCCTGCAACACTGGTGGTGCCGCCTTTGAACCCAGGTTGAATACCTTTGAAAAAGAGAGGGCAATGTATGATGATGAAGACGAGGATG
ATGAGGAGGAGAAGCCCTCTGGATGGTTGAAAATGTCATTGCTGTTTGTATTTGTTTTGCTGTCTGCTTCAGTTGCTTTGTTGGGGTCGGGCAGCCCTGGTCTTTTGCCACCTTCTCTGCAAAACAATGCATTGGCTACTGGTAAGTTTTATGACTCTGCAGTAAATGCTGTTAAAGATGTAGAAGGGTTTTGGAGAAAG
GGTTCTGTCAAAGATATGAATCATTCTCTTTTAGAGTATTCAAGGACAAAATTTGATGTCACTATAGAGAATGTCAAGTTCTGGTTTGATTCATCCTTGACCAAGCTAAAGATGCTAACAAATTCTGTCATGGAGAACTCCTTTGAGAAAACCCATAGTAGGAGTATGGAAGAAATGAGTAATCATCTTGGCTTGGAAAC
TATTGTAGAGAGGGACATCAAGGACCATAGCCTTCCCTCCGATGAGGGGTTACTGGAAGAGAAGGAGAGTAGCGCATCGGAGGGGTTACTGGAAGAGAAGGAGAGTAGCACAGAGGCTGCTCAGGAGGGATTTGATCGGTTTCTTGTCTCTTCAGTTTATAATGAGCAGTCCACCCAAATAGCCAAAGGATTTGAGGGAG
ACCGTGTTCTAGAAGGGAATACTAAGCTTGAAGGTGATCTTCAGAGTGCATCTGAGAACTCATCATCCTTTGGGTTGCAAGAAGAAGAGCTTCAGAGTGCATCTGAGAACCCATCATCCTTTGGGTTGCAAGAAGAAGAGAATAAAGTTGGAACACATGAACTTGTAGATTTCTACTCTGATATAAATGAAGAAAATGAT
ATAGATGGTAGAGAAGAGGGATTTGAGGAATCTATATCCTCATCTCCTTCATATATAATAGACATCTCTGCTAGTTTTGAGGAATCTCCTCCCATGGCCCATTCTGAAGAAGCAGATAATATCGATGAACATGCTTCTGAAGCAGTACACATTCCTGGGATTCCCATGGCCCCTTCTGAAGAAGCAGATAATATTGATGA
ACATGCTTCTGAAGCAGTACACATTCCTGGGATTCCTGAAACTACTGCCCTTCCTAACCCTGTCCAGATGTTTGCATCGGTTATGAGTGCAGATTCAGAAGTCAGTGAACTTTTTGTTCCTTTGGTTTCACAGAGCAACACAGAAGTCAAGGAAGAGCCTGAGGCATTGAGCATAGAGAGCCAAACACACGAGGATGTGA
CTGTTGAAGATGGAAATTACAATGCTTCTCATTCTAGCATAGATGATGAGTACGTTCTGGAGGAGTTTAAATCCACAGAAAGGACTGAATCTACGATTTCCCAAATCAAAGAGAGTGCAGTAGAAGAATCTTATCCACATTCTGCAAAGGATTTGAATGGTGCTGGAGTTGATACTGCCACCCTTCGTAAGAGCTCAGTA
AGAGAGCTTCTGCCGAGTGCAACTATAGGTGGTTTGTCAATTCTAGCAGCTACTGTATTAGCTGCCATTTATTTTGTTTCTGTCTCTAGGAAGAATTCTAAAATCTCTAGTGAAGTTGGAGGAAAATCAAACAGAGGGAGGTCAGTCAAAGTAACAAAAGAAGCAGGAAACATTTACTCCCCATACTCTAGGGTGCAGTT
TATTTCTCCCACGCAAAACATGATGGAGAAGCGTGTAAATTCCAGGCTTTCTCCGCCTCATGTGGAATTACTTGCTGATTATTTTCCAGCAGAGATGAGTAGCTCAGATAGAAATGTCAGTAGAGCGAGTACATATGACAATATTGCTCAGACATACGAAAGGAGGTCAAGAAGAGGAAACAGCATTTCCTCTGTAACTG
AGCAAGAATATACAGTTACACCAGACTTTTCCACAGACTCACCTTCTTATGGAAGCTTTACAACTTATGAGAACATTGTCCGAAAAGAGgtatgttaatattttgattctctcaatcctttttgcatttgttgttacattcttgtatgctttgtttatatttgccaatttcaaaacacaaagtcctggtaaatgagttca
tttagaaattatttagactttggtttcagaaaatcagagtgcttactccctattattttttaaaatatgagttcatataggaattgttttgaccgaggtctcagaaaatgagtgttaaatggcccaattctcttttcaaaaacttgacagcattgattgcttaaatctttagtttagtttggatttactaatttctaaat
ctagatcaagaaccttgtcacatgaattcctgtagaattggtaaatttggtgcacaacatcctctgggagtaacccttggccgaagttttgaaacatttgatgccaatgctaggatataagggctggttgtattctacagcttcttgaggctagataggtacttgcttttgtttctccaagatcgattctgtatctagaa
ggtaatgccagttgggttagggccaatctcctcacaagcccagcagagcttaaactaatttgtttattgcgttcaaaaattcctgttgtctaaagtgagactgacgctaaagggccattaaatttttttatgctttttagtttactttgagtcaacgagtctcaggattcagaacctgccctttaagggaagagactcta
gtgtcaggttatggttctgaattctgagactcattgacttaaagtaaactcaaaaacatcaaattttttaatggcccttaccctcagtgtgatatattttgcctaaaatttcaatatgtattagaccaaggaaagtaacaaagaatgtctgctgaatattctctttctatgaatttgagagcctgttcctccttgaactt
gaattctacaacttattgatgtaatgtagttggatgagcagGGTGGTCGAGATGAGGTGCACAAGGTTATGAAGGTAGTCACTCCGGTGAGAAGATCTAGTAGAATCCGCAAGCAAGCAATATCTCCACAGTGATGTGATGATGAAAACAAAGTGGGTTTTTGATACCCCTGGCTCATCCTATCCGATGAGCAATATTTC
TTTTATTGTTCTTGGGCATCATTTTATGTTTCAGAATTAAATTACTGCTTAAACAATGCATTCCTCATTGGAATGATGGCGACAAGGCAACACGATTACTTATGGGTTGTCCTAGTGCTCTGTAACTGAATGAATTAGTTAATTATAGATTTAACTGGTTTTTATTTTAGGGAAGAAATGTTTCCAGTGTTCATGCTCCA
AATGAATTGGTTGCTTTTATGCTTCATGATCTTATATCTTTTTCGGTGTTTGGAGAAGATTTTCACACATCCCGTTCAATGACAATGTTGTGTTTTCAATAAATTGCCCATGAGTTAGTTTGTTATCTACATCAGTAGAAGTAGATATGTTTGTGGAGTTGGCACTCTTAAATTGGTTTTCATGGCATTCTGTATTTATG
CAAAAATAAAAAATAAAAACAAGCTCGTGGAGTTAGGTAC

Intron 2 GTAAACATTA ... TCCATTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	20.88	180	44	51
A2	18.01	135	40.3	19
A3	19.71	167	45	37
A4	21.55	111	31	30
A5	22.4	97	28	28
A6	15.2	53	8	11
A7	16.55	232	43	49
A8	24.68	29	5	14
A9	23.57	201	44	80
A10	20.32	313	87.7	72

>TRINITY_DN98000_c0_g1_i1 from sample A5 / Example spliced isoform

CTCATCATCTTTTGCTTCGCGAGTTTTTGCCGTAGAAATCGCATTCTCAGAGCGATAGAACAGAATACAAGTCCATTTTCATGCGAGAAACCCGCTTCTGTTGAAGGGAAAGAGCGATTCAGAGCCTGATTATGGCATCTCCATTTAGATCTCCTTCTCCTGCTCTGGACAGGCCCAAGTCTGCCTTAACAAGCCCAGGG
AACGGTAATGGCCTACGGAAGAGCTTCAATGTCAATGTGCATCCCAAGGCTCCTGCGAAGAATCTCTCTGCCGTCACTCCGGCCAACAGCCCTGCAGACTATCCTCAGAGAGCACCATCTCCGTTCAGAGATTCAAATTTCAGACAATCGGAGGAGATCAAAGAGAACAGCGGGATTGAGAATATCCGGCCTCATAAGTC
TGCAAGCCTGAGGTCACCCACACCCAAAGGCATGAAGAGCTTCATGGCCCCAACATTTTCAGCTGCTTCCAAGGCTGCTGAACCCAGAAAGAGGGTTTTAATGGAGAAAAATGAGACTGTTGGGCATTCTGCTATTTCAATGCAATCACCGGCCAAGACCCTGGTGAAATCGCCGTCTGTGGCGAGGACCCAAATTTCGG
CCCTGCATGAGCCTATGATGGAAGATAGCCCGCTCGCTAGAGAAGATCATGAAGAAGTTTCAGTGGATTGTATGGAGGTGGACGAGATCCAAACCACGCCGCAGCCTAAAAGCAAGAACTGCAAGGTACTTTTTGAAGCTGGGGTTGTTCCCAATCCCATTGAGGGAAAGGAACCTAACCTTGAATCAAACGAGGATAGT
GAGTCTTTAACCTTTCTGGCTCAGTTTCCTGATCCTGGCGACGAGCCTATAATCCTTCCGCCCTATGATCCTAAGACTAATTATCTGTCTCCCCGGCCTCAATTTCTTCGTTATCGTCCAGAAAGAGACCTAGTATTGGATGTTGGGGAGCAAGGTTTTGGGGGTCATGGTGTTTTTGAATATGGACCCAAGAGCGGGCT
TACCGAAGTCGCAGAGATTGGGGAAATACAGTCCCTGGGAGAGCAAATGGCTTCTGTAGGCATATCCACCGATTGTGGAGAAATCCATGAAGAAATTCAAGATGTGGAAGAAATAGAAATAGCATCAGTTGATAAATCTGAAGAAAAGCCCTGCAACACTGGTGGTGCCGCCTTTGAACCCAGGTTGAATACCTTTGAAA
AAGAGAGGGCAATGTATGATGATGAAGACGAGGATGATGAGGAGGAGAAGCCCTCTGGATGGTTGAAAATGTCATTGCTGTTTGTATTTGTTTTGCTGTCTGCTTCAGTTGCTTTGTTGGGGTCGGGCAGCCCTGGTCTTTTGCCACCTTCTCTGCAAAACAATGCATTGGCTACTGGTAAGTTTTATGACTCTGCAGTA
AATGCTGTTAAAGATGTAGAAGGGTTTTGGAGAAAGGGTTCTGTCAAAGATATGAATCATTCTCTTTTAGAGTATTCAAGGACAAAATTTGATGTCACTATAGAGAATGTCAAGTTCTGGTTTGATTCATCCTTGACCAAGCTAAAGATGCTAACAAATTCTGTCATGGAGAACTCCTTTGAGAAAACCCATAGTAGGAG
TATGGAAGAAATGAGTAATCATCTTGGCTTGGAAACTATTGTAGAGAGGGACATCAAGGACCATAGCCTTCCCTCCGATGAGGGGTTACTGGAAGAGAAGGAGAGTAGCGCATCGGAGGGGTTACTGGAAGAGAAGGAGAGTAGCACAGAGGCTGCTCAGGAGGGATTTGATCGGTTTCTTGTCTCTTCAGTTTATAATG
AGCAGTCCACCCAAATAGCCAAAGGATTTGAGGGAGACCGTGTTCTAGAAGGGAATACTAAGCTTGAAGGTGATCTTCAGAGTGCATCTGAGAACTCATCATCCTTTGGGTTGCAAGAAGAAGAGCTTCAGAGTGCATCTGAGAACCCATCATCCTTTGGGTTGCAAGAAGAAGAGAATAAAGTTGGAACACATGAACTT
GTAGATTTCTACTCTGATATAAATGAAGAAAATGATATAGATGGTAGAGAAGAGGGATTTGAGGAATCTATATCCTCATCTCCTTCATATATAATAGACATCTCTGCTAGTTTTGAGGAATCTCCTCCCATGGCCCATTCTGAAGAAGCAGATAATATCGATGAACATGCTTCTGAAGCAGTACACATTCCTGGGATTCC
CATGGCCCCTTCTGAAGAAGCAGATAATATTGATGAACATGCTTCTGAAGCAGTACACATTCCTGGGATTCCTGAAACTACTGCCCTTCCTAACCCTGTCCAGATGTTTGCATCGGTTATCAGTGCAGATTCAGAAGTCAGTGAACTTTTTGTTCCTTTGGTTTCACAGAGCAACACAGAAGTCAAGGAAGAGCCTGAGG
CATTGAGCATAGAGAGCCAAACACACGAGGATGTGACTGTTGAAGATGGAAATTACAATGCTTCTCATTCTAGCATAGATGATGAGTACGTTCTGGAGGAGTTTAAATCCACAGAAAGGACTGAATCTACGATTTCCCAAATCAAAGAGAGTGCAGTAGAAGAATCTTATCCACATTCTGCAAAGGATTTGAATGGTGCT
GGAGTTGATACTGCCACCCTTCGTAAGAGCTCAGTAAGAGAGCTTCTGCCGAGTGCAACTATAGGTGGTTTGTCAATTCTAGCAGCTACTGTATTAGCTGCCATTTATTTTGTTTCTGTCTCTAGGAAGAATTCTAAAATCTCTAGTGAAGTTGGAGGAAAATCAAACAGAGGGAGGTCAGTCAAAGTAACAAAAGAAGC
AGGAAACATTTACTCCCCATACTCTAGGGTGCAGTTTATTTCTCCCACGCAAAACATGATGGAGAAGCGTGTAAATTCCAGGCTTTCTCCGCCTCATGTGGAATTACTTGCTGATTATTTTCCAGCAGAGATGAGTAGCTCAGATAGAAATGTCAGTAGAGCGAGTACATATGACAATATTGCTCAGACATACGAAAGGA
GGTCAAGAAGAGGAAACAGCATTTCCTCTGTAACTGAGCAAGAATATACAGTTACACCAGACTTTTCCACAGACTCACCTTCTTATGGAAGCTTTACAACTTATGAGAACATTGTCCGAAAAGAGGGTGGTCGAGATGAGGTGCACAAGGTTATGAAGGTAGTCACTCCGGTGAGAAGATCTAGTAGAATCCGCAAGCAA
GCAATATCTCCACAGTGATGTGATGATGAAAACAAAGTGGGTTTTTGATACCCCTGGCTCATCCTATCCGATGAGCAATATTTCTTTTATTGTTCTTGGGCATCATTTTATGTTTCAGAATTAAATTACTGCTTAAACAATGCATTCCTCATTGGAATGATGGCGACAAGGCAACACGATTACTTATGGGTTGTCCTAGT
GCTCTGTAACTGAATGAATTAGTTAATTATAGATTTAACTGGTTTTTATTTTAGGGAAGAAATGTTTCCAGTGTTCATGCTCCAAATGAATTGGTTGCTTTTATGCTTCATGATCTTATATCTTTTTCGGTGTTTGGAGAAGATTTTCACACATCCCGTTCAATGACG

>TRINITY_DN98000_c0_g1_i3 from sample A5 / Example unspliced isoform:

CTCATCATCTTTTGCTTCGCGAGTTTTTGCCGTAGAAATCGCATTCTCAGAGCGATAGAACAGAATACAAGTCCATTTTCATGCGAGAAACCCGCTTCTGTTGAAGGGAAAGAGCGATTCAGAGCCTGATTATGGCATCTCCATTTAGATCTCCTTCTCCTGCTCTGGACAGGCCCAAGTCTGCCTTAACAAGCCCAGGG
AACGGTAATGGCCTACGGAAGAGCTTCAATGTCAATGTGCATCCCAAGGCTCCTGCGAAGAATCTCTCTGCCGTCACTCCGGCCAACAGCCCTGCAGgtaaacattacagaaaaatcttgtaatggcttctgttggattaggcgatggtttggtatttgtatttatcttgacttgtgttgctttccattgcagACTATCC
TCAGAGAGCACCATCTCCGTTCAGAGATTCAAATTTCAGACAATCGGAGGAGATCAAAGAGAACAGCGGGATTGAGAATATCCGGCCTCATAAGTCTGCAAGCCTGAGGTCACCCACACCCAAAGGCATGAAGAGCTTCATGGCCCCAACATTTTCAGCTGCTTCCAAGGCTGCTGAACCCAGAAAGAGGGTTTTAATGG
AGAAAAATGAGACTGTTGGGCATTCTGCTATTTCAATGCAATCACCGGCCAAGACCCTGGTGAAATCGCCGTCTGTGGCGAGGACCCAAATTTCGGCCCTGCATGAGCCTATGATGGAAGATAGCCCGCTCGCTAGAGAAGATCATGAAGAAGTTTCAGTGGATTGTATGGAGGTGGACGAGATCCAAACCACGCCGCAG
CCTAAAAGCAAGAACTGCAAGGTACTTTTTGAAGCTGGGGTTGTTCCCAATCCCATTGAGGGAAAGGAACCTAACCTTGAATCAAACGAGGATAGTGAGTCTTTAACCTTTCTGGCTCAGTTTCCTGATCCTGGCGACGAGCCTATAATCCTTCCGCCCTATGATCCTAAGACTAATTATCTGTCTCCCCGGCCTCAATT
TCTTCGTTATCGTCCAGAAAGAGACCTAGTATTGGATGTTGGGGAGCAAGGTTTTGGGGGTCATGGTGTTTTTGAATATGGACCCAAGAGCGGGCTTACCGAAGTCGCAGAGATTGGGGAAATACAGTCCCTGGGAGAGCAAATGGCTTCTGTAGGCATATCCACCGATTGTGGAGAAATCCATGAAGAAATTCAAGATG
TGGAAGAAATAGAAATAGCATCAGTTGATAAATCTGAAGAAAAGCCCTGCAACACTGGTGGTGCCGCCTTTGAACCCAGGTTGAATACCTTTGAAAAAGAGAGGGCAATGTATGATGATGAAGACGAGGATGATGAGGAGGAGAAGCCCTCTGGATGGTTGAAAATGTCATTGCTGTTTGTATTTGTTTTGCTGTCTGCT
TCAGTTGCTTTGTTGGGGTCGGGCAGCCCTGGTCTTTTGCCACCTTCTCTGCAAAACAATGCATTGGCTACTGGTAAGTTTTATGACTCTGCAGTAAATGCTGTTAAAGATGTAGAAGGGTTTTGGAGAAAGGGTTCTGTCAAAGATATGAATCATTCTCTTTTAGAGTATTCAAGGACAAAATTTGATGTCACTATAGA
GAATGTCAAGTTCTGGTTTGATTCATCCTTGACCAAGCTAAAGATGCTAACAAATTCTGTCATGGAGAACTCCTTTGAGAAAACCCATAGTAGGAGTATGGAAGAAATGAGTAATCATCTTGGCTTGGAAACTATTGTAGAGAGGGACATCAAGGACCATAGCCTTCCCTCCGATGAGGGGTTACTGGAAGAGAAGGAGA
GTAGCGCATCGGAGGGGTTACTGGAAGAGAAGGAGAGTAGCACAGAGGCTGCTCAGGAGGGATTTGATCGGTTTCTTGTCTCTTCAGTTTATAATGAGCAGTCCACCCAAATAGCCAAAGGATTTGAGGGAGACCGTGTTCTAGAAGGGAATACTAAGCTTGAAGGTGATCTTCAGAGTGCATCTGAGAACTCATCATCC
TTTGGGTTGCAAGAAGAAGAGCTTCAGAGTGCATCTGAGAACCCATCATCCTTTGGGTTGCAAGAAGAAGAGAATAAAGTTGGAACACATGAACTTGTAGATTTCTACTCTGATATAAATGAAGAAAATGATATAGATGGTAGAGAAGAGGGATTTGAGGAATCTATATCCTCATCTCCTTCATATATAATAGACATCTC
TGCTAGTTTTGAGGAATCTCCTCCCATGGCCCATTCTGAAGAAGCAGATAATATCGATGAACATGCTTCTGAAGCAGTACACATTCCTGGGATTCCCATGGCCCCTTCTGAAGAAGCAGATAATATTGATGAACATGCTTCTGAAGCAGTACACATTCCTGGGATTCCTGAAACTACTGCCCTTCCTAACCCTGTCCAGA
TGTTTGCATCGGTTATCAGTGCAGATTCAGAAGTCAGTGAACTTTTTGTTCCTTTGGTTTCACAGAGCAACACAGAAGTCAAGGAAGAGCCTGAGGCATTGAGCATAGAGAGCCAAACACACGAGGATGTGACTGTTGAAGATGGAAATTACAATGCTTCTCATTCTAGCATAGATGATGAGTACGTTCTGGAGGAGTTT
AAATCCACAGAAAGGACTGAATCTACGATTTCCCAAATCAAAGAGAGTGCAGTAGAAGAATCTTATCCACATTCTGCAAAGGATTTGAATGGTGCTGGAGTTGATACTGCCACCCTTCGTAAGAGCTCAGTAAGAGAGCTTCTGCCGAGTGCAACTATAGGTGGTTTGTCAATTCTAGCAGCTACTGTATTAGCTGCCAT
TTATTTTGTTTCTGTCTCTAGGAAGAATTCTAAAATCTCTAGTGAAGTTGGAGGAAAATCAAACAGAGGGAGGTCAGTCAAAGTAACAAAAGAAGCAGGAAACATTTACTCCCCATACTCTAGGGTGCAGTTTATTTCTCCCACGCAAAACATGATGGAGAAGCGTGTAAATTCCAGGCTTTCTCCGCCTCATGTGGAAT
TACTTGCTGATTATTTTCCAGCAGAGATGAGTAGCTCAGATAGAAATGTCAGTAGAGCGAGTACATATGACAATATTGCTCAGACATACGAAAGGAGGTCAAGAAGAGGAAACAGCATTTCCTCTGTAACTGAGCAAGAATATACAGTTACACCAGACTTTTCCACAGACTCACCTTCTTATGGAAGCTTTACAACTTAT
GAGAACATTGTCCGAAAAGAGGGTGGTCGAGATGAGGTGCACAAGGTTATGAAGGTAGTCACTCCGGTGAGAAGATCTAGTAGAATCCGCAAGCAAGCAATATCTCCACAGTGATGTGATGATGAAAACAAAGTGGGTTTTTGATACCCCTGGCTCATCCTATCCGATGAGCAATATTTCTTTTATTGTTCTTGGGCATC
ATTTTATGTTTCAGAATTAAATTACTGCTTAAACAATGCATTCCTCATTGGAATGATGGCGACAAGGCAACACGATTACTTATGGGTTGTCCTAGTGCTCTGTAACTGAATGAATTAGTTAATTATAGATTTAACTGGTTTTTATTTTAGGGAAGAAATGTTTCCAGTGTTCATGCTCCAAATGAATTGGTTGCTTTTAT
GCTTCATGATCTTATATCTTTTTCGGTGTTTGGAGAAGATTTTCACACATCCCGTTCAATGACG