sp|Q9M884|MPI1_ARATH ----> Mannose-6-phosphate isomerase 1

Intron 1 GTAAATATCG ... CCTTTCCCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	48.72	10	9	10
A2	47.37	10	10	8
A3	56.67	13	13	21
A4	67.57	6	21	4
A5	86.67	1	9	4
A6	54.84	14	19	15
A7	48.28	15	13	15
A8	31.43	12	4	7
A9	44.83	8	8	5
A10	62.26	10	20	13

>TRINITY_DN102025_c0_g1_i2 from sample A1 / Example spliced isoform

GCTTGACGCATCCTAACCCCTATAACTTGAGGTGGGATAGCTCCTTCATTGAGAGGGCTTTGACATGGCTTTGGACCGAACTGAGTTTGTTAGTTTGGGCTGGACTTGCCTTTGAATCAGACTCAGTTTGCTAGTTTGGCCCAAACTCGATCAGACATGTCCAGCAACTTGAGCAGGGAGGCAGTGCCCAATCTGCCCAT
CAAGCTCACCAGAATTCGATACGGAGATACATATTTCTAATCAATCTCGTGCATTCGGAAGAGATGTCACCATTTTGTCGGCTTTTGGAATCTATTCACATTGTGTGACAGCTCCCATGTCGCTATGCGCCATTGAAATGCCCGCTCTCTCCCTTTAAAAATTCCTACATATCGAGGCGTTTAATGGGCCGTTCGTACTC
TGAGATTTGTGCGAGAAATCAATTGAAGGATGGCAAGAGTAGGAGAATTGAAGCCCCTGCGGTTGCGGTGCGTAGTCCAGCACTACGAATGGGGGCGACATGGGTCTGCCAGCGAAGTTGGCAGGCTCTATTCGCTCATAACTGAATCCCCGATAATGGAAGACAAGCCCTATGCAGAGCTCTGGATGGGTACCCATGAT
TCGGGGCATTCCCTCGTTATATTACAAGGAGGAGATCAAGTGCCCTTGAAGAGGTGGCTGGAGGAGCATCCTGAGGCGCTCGGAGACAAGGTCGTGGAGCGATGGGGAATCGATCTGCCCTTTCTTTTCAAGGTTCTTTCAGTAGGAAAAGCTTTATCCATACAAGCACATCCTGACAAGGATCTTGCTCGGCTTTTACA
TGAGGCTCAGCCGAAGAATTACAAGGACTCTAATCATAA

>TRINITY_DN102025_c0_g1_i1 from sample A1 / Example unspliced isoform:

GCTTGACGCATCCTAACCCCTATAACTTGAGGTGGGATAGCTCCTTCATTGAGAGGGCTTTGACATGGCTTTGGACCGAACTGAGTTTGTTAGTTTGGGCTGGACTTGCCTTTGAATCAGACTCAGTTTGCTAGTTTGGCCCAAACTCGATCAGACATGTCCAGgtaaatatcgaaatttttttctgttttatttgtcca
tcttttcggctgagattttgtcctggtccgagccatttgccaaatgtggcccacgaggagtaaagtatttttttcagcgttcgatgcactcgtggcttataccatgacctctacttttcgctccctttcccagCAACTTGAGCAGGGAGGCAGTGCCCAATCTGCCCATCAAGCTCACCAGAATTCGATACGGAGATACA
TATTTCTAATCAATCTCGTGCATTCGGAAGAGATGTCACCATTTTGTCGGCTTTTGGAATCTATTCACATTGTGTGACAGCTCCCATGTCGCTATGCGCCATTGAAATGCCCGCTCTCTCCCTTTAAAAATTCCTACATATCGAGGCGTTTAATGGGCCGTTCGTACTCTGAGATTTGTGCGAGAAATCAATTGAAGGAT
GGCAAGAGTAGGAGAATTGAAGCCCCTGCGGTTGCGGTGCGTAGTCCAGCACTACGAATGGGGGCGACATGGGTCTGCCAGCGAAGTTGGCAGGCTCTATTCGCTCATAACTGAATCCCCGATAATGGAAGACAAGCCCTATGCAGAGCTCTGGATGGGTACCCATGATTCGGGGCATTCCCTCGTTATATTACAAGGAG
GAGATCAAGTGCCCTTGAAGAGGTGGCTGGAGGAGCATCCTGAGGCGCTCGGAGACAAGGTCGTGGAGCGATGGGGAATCGATCTGCCCTTTCTTTTCAAGGTTCTTTCAGTAGGAAAAGCTTTATCCATACAAGCACATCCTGACAAGGATCTTGCTCGGCTTTTACATGAGGCTCAGCCGAAGAATTACAAGGACTCT
AATCATAA

Intron 2 GTAAATATCG ... TTTTTTTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	41.18	10	9	5
A2	46.67	8	10	4
A3	76.19	5	13	19
A4	87.1	2	21	6
A5	56.25	7	9	9
A6	50	16	19	13
A7	45.16	17	13	15
A8	75	1	4	2
A9	58.62	6	8	9
A10	50.65	19	20	19

>TRINITY_DN102025_c0_g1_i3 from sample A1 / Example spliced isoform

GCTTGACGCATCCTAACCCCTATAACTTGAGGTGGGATAGCTCCTTCATTGAGAGGGCTTTGACATGGCTTTGGACCGAACTGAGTTTGTTAGTTTGGGCTGGACTTGCCTTTGAATCAGACTCAGTTTGCTAGTTTGGCCCAAACTCGATCAGACATGTCCAGCGTTCGATGCACTCGTGGCTTATACCATGACCTCTA
CTTTTCGCTCCCTTTCCCAGCAACTTGAGCAGGGAGGCAGTGCCCAATCTGCCCATCAAGCTCACCAGAATTCGATACGGAGATACATATTTCTAATCAATCTCGTGCATTCGGAAGAGATGTCACCATTTTGTCGGCTTTTGGAATCTATTCACATTGTGTGACAGCTCCCATGTCGCTATGCGCCATTGAAATGCCCG
CTCTCTCCCTTTAAAAATTCCTACATATCGAGGCGTTTAATGGGCCGTTCGTACTCTGAGATTTGTGCGAGAAATCAATTGAAGGATGGCAAGAGTAGGAGAATTGAAGCCCCTGCGGTTGCGGTGCGTAGTCCAGCACTACGAATGGGGGCGACATGGGTCTGCCAGCGAAGTTGGCAGGCTCTATTCGCTCATAACTG
AATCCCCGATAATGGAAGACAAGCCCTATGCAGAGCTCTGGATGGGTACCCATGATTCGGGGCATTCCCTCGTTATATTACAAGGAGGAGATCAAGTGCCCTTGAAGAGGTGGCTGGAGGAGCATCCTGAGGCGCTCGGAGACAAGGTCGTGGAGCGATGGGGAATCGATCTGCCCTTTCTTTTCAAGGTTCTTTCAGTA
GGAAAAGCTTTATCCATACAAGCACATCCTGACAAGGATCTTGCTCGGCTTTTACATGAGGCTCAGCCGAAGAATTACAAGGACTCTAATCATAA

>TRINITY_DN102025_c0_g1_i1 from sample A1 / Example unspliced isoform:

GCTTGACGCATCCTAACCCCTATAACTTGAGGTGGGATAGCTCCTTCATTGAGAGGGCTTTGACATGGCTTTGGACCGAACTGAGTTTGTTAGTTTGGGCTGGACTTGCCTTTGAATCAGACTCAGTTTGCTAGTTTGGCCCAAACTCGATCAGACATGTCCAGgtaaatatcgaaatttttttctgttttatttgtcca
tcttttcggctgagattttgtcctggtccgagccatttgccaaatgtggcccacgaggagtaaagtatttttttcagCGTTCGATGCACTCGTGGCTTATACCATGACCTCTACTTTTCGCTCCCTTTCCCAGCAACTTGAGCAGGGAGGCAGTGCCCAATCTGCCCATCAAGCTCACCAGAATTCGATACGGAGATACA
TATTTCTAATCAATCTCGTGCATTCGGAAGAGATGTCACCATTTTGTCGGCTTTTGGAATCTATTCACATTGTGTGACAGCTCCCATGTCGCTATGCGCCATTGAAATGCCCGCTCTCTCCCTTTAAAAATTCCTACATATCGAGGCGTTTAATGGGCCGTTCGTACTCTGAGATTTGTGCGAGAAATCAATTGAAGGAT
GGCAAGAGTAGGAGAATTGAAGCCCCTGCGGTTGCGGTGCGTAGTCCAGCACTACGAATGGGGGCGACATGGGTCTGCCAGCGAAGTTGGCAGGCTCTATTCGCTCATAACTGAATCCCCGATAATGGAAGACAAGCCCTATGCAGAGCTCTGGATGGGTACCCATGATTCGGGGCATTCCCTCGTTATATTACAAGGAG
GAGATCAAGTGCCCTTGAAGAGGTGGCTGGAGGAGCATCCTGAGGCGCTCGGAGACAAGGTCGTGGAGCGATGGGGAATCGATCTGCCCTTTCTTTTCAAGGTTCTTTCAGTAGGAAAAGCTTTATCCATACAAGCACATCCTGACAAGGATCTTGCTCGGCTTTTACATGAGGCTCAGCCGAAGAATTACAAGGACTCT
AATCATAA