sp|Q9SU71|EDSBC_ARATH ----> Protein EDS1B

Intron 1 GTAGGGCTCG ... TATCTTTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	87.45	8	43	68.5
A2	92.13	6	49.5	91
A3	88.2	11	56	108.5
A4	86.66	8	47.5	56.5
A5	97.45	1	24	52.5
A6	87.27	41.5	218	351
A7	88.16	51	235	524.5
A8	87.09	47.5	226.5	414.5
A9	87.11	49	224	438.5
A10	85.01	53	182.5	418.5

>TRINITY_DN45028_c4_g2_i5 from sample A9 / Example spliced isoform

GGAGCGCAGGGCCACACAAGCACACATACAAACAGAGGGATCTTCCCATCATGTGGCTATAGAGCAACTCCATCTGGAGTTGGAACAGCATTGCAATCTAATGAATTGCCAGGCTTTACTTTGATTGCTCTGTTTCGTAGTTTTGCCTTTTCAGCTTTCTGCATGCTAGTTCATTATTGTAATGATCATGCACAGCTTGC
CTTGCGAAGAGCTGGAGAAACTGAGAACAAATATAGCACAAATGTTGGCAAGTTAGCTATTGACTTAAGCCAAAGACAAAGTACTATGGCAGAGCTTGAGTGGTATAAAGAGGGTTGTGAGAAGAACGGTGGCATCGGATATTATGATTCATTGAAGAACCAGAATGAAAAGAAAGATATCGATGCTAATTTGCGTAGGA
TGGAAGACCCGATCGTCATAAAGTTCTTCAGAAATGGATGGAAGCTAAAGAAAAAACTCGCAGTTCAAGAGGTTCAAAACCCCGCAC

>TRINITY_DN45028_c4_g2_i10 from sample A9 / Example unspliced isoform:

CCCGAAACAAAAGAAGCTCAAACCCATTGTATTTGTGGGTCATTCCTTAGGAGGGGCAGTTGCGACATTGGCCACACTATGGGTACTGGAAAAGAGGCTGAGGCAAAGCTCTCCCTTTTGTATCACATTTGGTTGCCCTTTAGTGGGAGATGAAGGACTTGTTGAAGCAGTTGGTCGTGAGAACTGGGGAGGCAACTTTT
TGCATGTGGTTTCCCAGCATGATATTGTTCCTCGGATGCTCCTTGCACCGCTTGAGTCCATTAATGAGCCTTTGACTGCTATTTTGCCGTATTGGCGTGGCAAAGTCGCAGATTCTTCTCTTCAAGATGTTTGTAGGACTCTTCTGAATACTGTCCTGCAGCATACTAATACAGTGGCAAACTATGGGCATGATTATTTA
AAGGGGTCAGATGGGGTTATAAGGAGAAGTCCATACAAGCCTTTTGGCTCTTACATGTTTTGCTCCATAAATGGAGCAGCTTGTATTGACAATTCTGAGACCGTTTTGAAGATGCTTCATTGGACAATGCAGAGCTATCAAAAGCTTTCTGATAATATTACACAGGAGTGTTTTTCAGAGCATATAGGTTATGGTTCTGC
TTTGAAATTTGTCAAAGAAAAATCCATAAGTGGGATAAGATTTACAAATCCTGAGTCAAAATCCTCTTTCGAGATGGGGATATCATTGCAGTTAGAAGCTATTGGTGTTGGAGCGCAGAATGATCATGCACAGCTTGCCTTGCGAAGAGCTGGAGAAACTGAGAACAAATATAGCACAAATGTTGGCAAGTTAGCTATTG
ACTTAAGCCAAAGACAAAGTACTATGGCAGAGCTTGAGTGGTATAAAGAGGGTTGTGAGAAGAACGGTGGCATCGGATATTATGATTCATTGAAGAACCAGAATGAAAAGAAAGATATCGATGCTAATTTGCGTAGGgtagggctcgctctgttttgggatgacattattgacatgtgggagaatcatgaattgccaaat
gattttgaatcgcaaaataagtggataaatgctggcaatacatatagaaaacttgtagagcctctggatattgcatattattatcgcactaatgccaacggcaactatctttcagATGGAAGACCCGATCGTCATAAAGTTCTTCAGAAATGGATGGAAGCTAAAGAAAAAACTCGCAGTTCAAGAGGTTCAAAACCCCG
CAC

Intron 2 GTAAATTATT ... GATCAATCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	30.27	29.5	9.5	16
A2	23.09	35	12	9
A3	27.49	31	11.5	12
A4	21.43	28.5	4	11.5
A5	9.68	14	0	3
A6	24.38	44.5	18.5	10
A7	26.61	50.5	17.5	19
A8	41.81	18.5	10.5	16
A9	20.75	46	12	12
A10	26.02	41	22.5	6

>TRINITY_DN46428_c4_g1_i10 from sample A8 / Example spliced isoform

TGTTGTGTAGCAAATCAAATCAGGCAGTAAGAGCAGATCATGGAAGCAGACTCGTCTAACCTGCAATTCACTGCTGGTCAGGATATTGCCACTTTCCTTGTTTCATGCGGAATACTGTCCAAGGCTTGGGAAGAGTCACGTCTTGCAAACAACTCAGAGAATTATATTCTGAATGAGTATGAAGGCGTTGCATATGTTTC
CTTCTCATTTCATAAAATAGAACGTTTCGTTGCCAATAAGAATAAATATGGAGAGGGCAATATACAAAACGATAACATGGGCTTCTATGGTTGTCTAAAGGGTAATGATGGTCAACCGACCCTCGCCCATCAGGGAGCTATCAAGTTGTTCCTCCATGTTATGGAGAATATAGATTTACAAGAAAAGTTGCAAATTTATA
TGGATCCTAAGCAAAAGAAACTCAAACCCATTATTTTTGTGGGTCATTCCTTAGGAGGAGCAGTTGCAACCTTGGCCACACTATGGGTACTGGAAAAGAGGCTGAGGCAAAGCTCTTCTTTTTGTATAACATTTGGTTCTCCTTTAGTGGGAGATGTAAGTTGACACAGTTGGCCGTGAAAATTGGACAAGCAACTTTTT
GCATGTGGTTTCCCAGCATGATATTGTTCCTTGGATGCTCCTTGCACCATTTAATTCAATTGCTGATCCCTTGACAACTATTTTGCCTTGTTGGCAGGGCCTAATAGATAATGACAGTAACAATGTAGTTGATTCTTTTATTCAAGATGCCTGTAGGACTCTTCTTAACAATGTTATGCAGTATACTTATACAATAGTAA
ACTATGGGCTCGATTCTCTAAAGGAGTTAGATGGAGTTGTCAAGAGAAGCCCATACATACCTTTTGGCACATACATGTTTTGTTCACGTGAGGGAGTAGCTTGTATTGAGAATTCTAAGATCGTTTTGAAGATGCTTCATTTGACAATGCAGAGCCATGAAAAACCTTCTAACAAGATTGTACAGGACTGTTTTTCG

>TRINITY_DN46428_c4_g1_i8 from sample A8 / Example unspliced isoform:

TGTTGTGTAGCAAATCAAATCAGGCAGTAAGAGCAGATCATGGAAGCAGACTCGTCTAACCTGCAATTCACTGCTGGTCAGGATATTGCCACTTTCCTTGTTTCATGCGGAATACTGTCCAAGGCTTGGGAAGAGTCACGTCTTGCAAACAACTCAGAGAATTATATTCTGAATGAGTATGAAGGCGTTGCATATGTTTC
CTTCTCATTTCATAAAATAGAACGTTTCGTTGCCAATAAGAATAAATATGGAGAGGGCAATATACAAAACGATAACATGGGCTTCTATGGTTGTCTAAAGGGTAATGATGGTCAACCGACCCTCACCCATCAGGGAGCTCTCAAGTTGTTCCTCCATGTTATGGAGAATATAGATTTACAAGAAAAGgtaaattattttt
tccaagcacaactgtcaggacaatttgatcttaatttcatagaaggaaggtttatttcactaattagaggtgaaatttttggaaaaggaaaagtaacattgtgttgatgtattccatgatcaatcagTTGCAAATTTATATGGATCCTAAGCAAAAGAAACTCAAACCCATTATTTTTGTGGGTCATTCCTTAGGAGGAG
CAGTTGCAACCTTGGCCACACTATGGGTACTGGAAAAGAGGCTGAGGCAAAGCTCTTCTTTTTGTATAACATTTGGTTCTCCTTTAGTGGGAGATGTAAGTTGACACAGTTGGCCGTGAAAATTGGACAAGCAACTTTTTGCATGTGGTTTCCCAGCATGATATTGTTCCTTGGATGCTCCTTGCACCATTTAATTCAAT
TGCTGATCCCTTGACAACTATTTTGCCTTGTTGGCAGGGCCTAATAGATAATGACAGTAACAATGTAGTTGATTCTTTTATTCAAGATGCCTGTAGGACTCTTCTTAACAATGTTATGCAGTATACTTATACAATAGTAAACTATGGGCTCGATTCTCTAAAGGAGTTAGATGGAGTTGTCAAGAGAAGCCCATACATAC
CTTTTGGCACATACATGTTTTGTTCACGTGAGGGAGTAGCTTGTATTGAGAATTCTAAGATCGTTTTGAAGATGCTTCATTTGACAATGCAGAGCCATGAAAAACCTTCTAACAAGATTGTACAGGACTGTTTTTCG

Intron 3 GTAAATTATT ... CATTCCTTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	73.91	12	10	34
A2	83.33	7	12	35
A3	82.61	12	10	57
A4	80	10	4	40
A5	91.67	3	0	33
A6	52.86	33	19	37
A7	73.21	30	18	82
A8	73.47	13	11	36
A9	71.28	27	12	67
A10	62.67	28	22	47

>TRINITY_DN46428_c4_g1_i1 from sample A8 / Example spliced isoform

TGTTGTGTAGCAAATCAAATCAGGCAGTAAGAGCAGATCATGGAAGCAGACTCGTCTAACCTGCAATTCACTGCTGGTCAGGATATTGCCACTTTCCTTGTTTCATGCGGAATACTGTCCAAGGCTTGGGAAGAGTCACGTCTTGCAAACAACTCAGAGAATTATATTCTGAATGAGTATGAAGGCGTTGCATATGTTTC
CTTCTCATTTCATAAAATAGAACGTTTCGTTGCCAATAAGAATAAATATGGAGAGGGCAATATACAAAACGATAACATGGGCTTCTATGGTTGTCTAAAGGGTAATGATGGTCAACCGACCCTCACCCATCAGGGAGCTCTCAAGTTGTTCCTCCATGTTATGGAGAATATAGATTTACAAGAAAAGGAGGAGCAGTTGC
AACCTTGGCCACACTATGGGTACTGGAAAAGAGGCTGAGGCAAAGCTCTTCTTTTTGTATAACATTTGGTTCTCCTTTAGTGGGAGATGTAAGTTGACACAGTTGGCCGTGAAAATTGGACAAGCAACTTTTTGCATGTGGTTTCCCAGCATGATATTGTTCCTTGGATGCTCCTTGCACCATTTAATTCAATTGCTGAT
CCCTTGACAACTATTTTGCCTTGTTGGCAGGGCCTAATAGATAATGACAGTAACAATGTAGTTGATTCTTTTATTCAAGATGCCTGTAGGACTCTTCTTAACAATGTTATGCAGTATACTTATACAATAGTAAACTATGGGCTCGATTCTCTAAAGGAGTTAGATGGAGTTGTCAAGAGAAGCCCATACATACCTTTTGG
CACATACATGTTTTGTTCACGTGAGGGAGTAGCTTGTATTGAGAATTCTAAGATCGTTTTGAAGATGCTTCATTTGACAATGCAGAGCCATGAAAAACCTTCTAACAAGATTGTACAGGACTGTTTTTCG

>TRINITY_DN46428_c4_g1_i8 from sample A8 / Example unspliced isoform:

TGTTGTGTAGCAAATCAAATCAGGCAGTAAGAGCAGATCATGGAAGCAGACTCGTCTAACCTGCAATTCACTGCTGGTCAGGATATTGCCACTTTCCTTGTTTCATGCGGAATACTGTCCAAGGCTTGGGAAGAGTCACGTCTTGCAAACAACTCAGAGAATTATATTCTGAATGAGTATGAAGGCGTTGCATATGTTTC
CTTCTCATTTCATAAAATAGAACGTTTCGTTGCCAATAAGAATAAATATGGAGAGGGCAATATACAAAACGATAACATGGGCTTCTATGGTTGTCTAAAGGGTAATGATGGTCAACCGACCCTCACCCATCAGGGAGCTCTCAAGTTGTTCCTCCATGTTATGGAGAATATAGATTTACAAGAAAAGgtaaattattttt
tccaagcacaactgtcaggacaatttgatcttaatttcatagaaggaaggtttatttcactaattagaggtgaaatttttggaaaaggaaaagtaacattgtgttgatgtattccatgatcaatcagttgcaaatttatatggatcctaagcaaaagaaactcaaacccattatttttgtgggtcattccttagGAGGAG

CAGTTGCAACCTTGGCCACACTATGGGTACTGGAAAAGAGGCTGAGGCAAAGCTCTTCTTTTTGTATAACATTTGGTTCTCCTTTAGTGGGAGATGTAAGTTGACACAGTTGGCCGTGAAAATTGGACAAGCAACTTTTTGCATGTGGTTTCCCAGCATGATATTGTTCCTTGGATGCTCCTTGCACCATTTAATTCAAT
TGCTGATCCCTTGACAACTATTTTGCCTTGTTGGCAGGGCCTAATAGATAATGACAGTAACAATGTAGTTGATTCTTTTATTCAAGATGCCTGTAGGACTCTTCTTAACAATGTTATGCAGTATACTTATACAATAGTAAACTATGGGCTCGATTCTCTAAAGGAGTTAGATGGAGTTGTCAAGAGAAGCCCATACATAC
CTTTTGGCACATACATGTTTTGTTCACGTGAGGGAGTAGCTTGTATTGAGAATTCTAAGATCGTTTTGAAGATGCTTCATTTGACAATGCAGAGCCATGAAAAACCTTCTAACAAGATTGTACAGGACTGTTTTTCG