NA ----> NA

Intron 1 GTAATATATT ... GTGTTCATAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	4.76	20	0	1
A2	0	9	0	0
A3	0	7	0	0
A4	0	19	0	0
A5	27.78	13	0	5
A6	15	51	0	9
A7	10.96	65	0	8
A8	29.55	62	2	26
A9	28.26	33	0	13
A10	8.97	71	0	7

>TRINITY_DN35515_c0_g1_i3 from sample A8 / Example spliced isoform

GGGAGATATTTTGGAGGCTTCCCAAGCTTGTTATTCAAATACCGATGGTTTCGGACTTTTCTTGGCTGACCGTGCTGTTGGCAGAGATATTTTATGGCCTGAGAACGCCCTTTTTTTCTGCCATTGGAAGGATCCAGATTTCTATATATATAAATATCAGGGTGTATATCTGATTTTTGTGGGCCTCTGGTTGGAGCTTT
CATGTGATGGTGGTGATAATACAGGATATGAATATATGAGCAGCAAATGCTCGCGGGGTTCTATTGATTTCGATCTTGAAAGTGAGGCCGCCATGTCTGAGAATGATGAATGTATGGATGCTAAAATTTCTTCATCCTTCAGATTCATGGCAGGTGCAGCAGATCGCCAAGCTCAACAAGCGATTCATCGCCAACCCCAA
CAACATGCCAACATGGTTTGGGCAGATCCTACAATATCAGAAAGTTCTTGGACTGATGAAAAACATAGCCTGTATCTGAATTCGATAGAGGAGGCCTTTGTTAAGAGCCTCCATGAACGGGGGGGACATTATACATATTCCAGTAAAAGATGGGAGGCTGAGAGGATAACTCCCTGTGTGTCTAGCAAGGGCTTATACGA
GGATGTTCATTCTGGGGTTCCATGTGATTACCGGCCATTTCTATTTCCTGAGGAGAATGGGGCTGAAAGTCAAAGGCGTTGTAGCCATGAAGATATTCAAAATAGAACAAAACGATCGAGATTGGAGGATATATGCATCAAACAATTAGGCCTGCAAGAAAAATATATGAATGGGAAAGCAGATAACTGCCAATCTGAGA
AGGAACCAGTTGGAGAAGAAACCACAGAAAGAGGAACTATTCAAATGCAATGTTTTAAAGATACTTCTAAGCATGATCATGGAGGCCAAGACCCAGAAACCTCAGAACGGTACATGCAGGAGGATCAGGTTGTTCCATCCACGGAGAATACTTCTAAGAGCTCTCCATAGACAGCAGGATTATCTTCTCTTCTTTTCTAT
TGTCACCCCAAAAGGAAACTGAAATTGTCAGGATGAAAATGGTAGAAATCTGTAATAGACACAGAATGTAATGTTGTAGGCCGAGCTATTCAGAGGAACCAGGATTGTCTTCTTTTCTATGGTCACCAAAAAGGTATGATGAAAATGCTGGAGCCTATAACAAAAGTCTGAACAATTTTGGCTTATTGTATATAAGGGCT
CTTGGACATCAGTTTTGTTTTTCTTGGTAAACCAGCCTGATCAACTCTTTGAAGTTCTTGAATGAATGTCAATGGAAGTTACAAGGAAACGCTTAGTATTGAATGTAAATGGAAGTTACAATGAAATACTTATTATTGACAATAATGAATGTCAATGAAAGTTATTATATTATCTAGCCCTTGGATATTAATTTTGTTTT
TCTTCGTAAACAAGCTTATCAACTCTTTGAAGTTCATGAATGAATGTCAATGGAAGTTACAAGGAAACACCTCTTTGAAGTTCATGAATGAATGTCAATGGAAGTTACAAGGAAAC

>TRINITY_DN35515_c0_g1_i2 from sample A8 / Example unspliced isoform:

CCCCAACAACATGCCAACATGgtaatatattgtagagcgtaggatctgtgcaatcgccattaatgccattgtagtctgttttattgatttgtttttaatttgtgttcatagGTTTGGGCAGATCCTACAATATCAGAAAGTTCTTGGACTGATGAAAAACATAGCCTGTATCTGAATTCGATAGAGGAGGCCTTTGTTAA
GAGCCTCCATGAACGGGGGGGACATTATACATATTCCAGTAAAAGATGGGAGGCTGAGAGGATAACTCCCTGTGTGTCTAGCAAGGGCTTATACGAGGATGTTCATTCTGGGGTTCCATGTGATTACCGGCCATTTCTATTTCCTGAGGAGAATGGGGCTGAAAGTCAAAGGCGTTGTAGCCATGAAGATATTCAAAATA
GAACAAAACGATCGAGATTGGAGGATATATGCATCAAACAATTAGGCCTGCAAGAAAAATATATGAATGGGAAAGCAGATAACTGCCAATCTGAGAAGGAACCAGTTGGAGAAGAAACCACAGAAAGAGGAACTATTCAAATGCAATGTTTTAAAGATACTTCTAAGCATGATCATGGAGGCCAAGACCCAGAAACCTCA
GAACGGTACATGCAGGAGGATCAGGTTGTTCCATCCACGGAGAATACTTCTAAGAGCTCTCCATAGACAGCAGGATTATCTTCTCTTCTTTTCTATTGTCACCCCAAAAGGAAACTGAAATTGTCAGGATGAAAATGGTAGAAATCTGTAATAGACACAGAATGTAATGTTGTAGGCCGAGCTATTCAGAGGAACCAGGA
TTGTCTTCTTTTCTATGGTCACCAAAAAGGTATGATGAAAATGCTGGAGCCTATAACAAAAGTCTGAACAATTTTGGCTTATTGTATATAAGGGCTCTTGGACATCAGTTTTGTTTTTCTTGGTAAACCAGCCTGATCAACTCTTTGAAGTTCTTGAATGAATGTCAATGGAAGTTACAAGGAAACGCTTAGTATTGAAT
GTAAATGGAAGTTACAATGAAATACTTATTATTGACAATAATGAATGTCAATGAAAGTTATTATATTATCTAGCCCTTGGATATTAATTTTGTTTTTCTTCGTAAACAAGCTTATCAACTCTTTGAAGTTCATGAATGAATGTCAATGGAAGTTACAAGGAAACACCTCTTTGAAGTTCATGAATGAATGTCAATGGAAG
TTACAAGGAAAC

Intron 2 GTGCACTTTT ... GGTCTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	59.18	10	8	21
A2	87.5	1	6	8
A3	79.49	4	23	8
A4	82.61	2	8	11
A5	63.64	6	9	12
A6	63.64	6	11	10
A7	74.28	16.8	59	38
A8	71.72	14	28	43
A9	75	6	25	11
A10	67.01	16	43	22

>TRINITY_DN36626_c0_g1_i2 from sample A6 / Example spliced isoform

GCGGGGGGACCGTGCTGTTGGCAGAGATATTTTATGGCCTGAGAACGCCCTTTTTTTCTGCCATTGGAAGGATCCAGATTTCTATATATATAAATATCAGGGTGTATATCTGATTTTTGTGGGCCTCTGGTTGGAGCTTTCATGTGATGGTGGTGATAATACAGGATATGAATATATGAGCAGCAAATGCTCGCGGGGTT
CTATTGATTTCGATCTTGAAAGTGAGGCCGCCATGTCTGAGAATGATGAATGTATGGATGCTAAAATTTCTTCATCCTTCAGATTCATGGCAGGTGCAGCAGATCGCCAAGCTCAACAAGCGATTCATCGCCAACCCCAACAACATGCCAACATGGTTTGGGCAGATCCTACAATATCAGAAAGTTCTTGGACTGATGAA
AAACATAGCCTGTATCTGAATTCGATAGAGGAGGCCTTTGTTAAGAGCCTCCATGAACGGGGGGGACATTATACATATTCCAGTAAAAGATGGGAGGCTGAGAGGATAACTCCCTGTGTGTCTAGCAAGGGCTTATACGAGGATGTTCATTCTGGGGTTCCATGTGATTACCGGCCATTTCTATTTCCTGAGGAGAATGG
GGCTGAAAGTCAAAGGCGTTGTAGCCATGAAGATATTCAAAATAGAACAAAACGATCGAGATTGGAGGATATATGCATCAAACAATTAGGCCTGCAAGAAAAATATATGAATGGGAAAGCAGATAACTGCCAATCTGAGAAGGAACCAGTTGGAGAAGAAACCACAGAAAGAGGAACTATTCAAATGCAATGTTTTAAAG
ATACTTCTAAGCATGATCATGGAGGCCAAGACCCAGAAACCTCAGAACGGTACATGCAGGAGGATCAGGTTGTTCCATCCACGGAGAATACTTCTAAGAGCTCTCCATAGACAGCAGGATTATCTTCTCTTCTTTTCTATTGTCACCCCAAAAGGAAACTGAAATTGTCAGGATGAAAATGGTAGAAATCTGTAATAGAC
ACAGAATGTAATGTTGTAGGCCGAGCTATTCAGAGGAACCAGGATTGTCTTCTTTTCTATGGTCACCAAAAAGGTATGATGAAAATGCTGGAGCCTATAACAAAAGTCTGAACAATTTTGGCTTATTGTATATAAGGGCTCTTGGACATCAGTTTTGTTTTTCTTGGTAAACCAGCCTGATCAACTCTTTGAAGTTCTTG
AATGAATGTCAATGGAAGTTACAAGGAAACGCTTAGTATTGAATGTAAATGGAAGTTACAATGAAATACTTATTATTGACAATAATGAATGTCAATGAAAGTTATTATATTATCTAGCCCTTGGATATTAATTTTGTTTTTCTTCGTAAACAAGCTTATCAACTCTTTGAAGTTCATGAATGAAT

>TRINITY_DN36626_c0_g1_i1 from sample A6 / Example unspliced isoform:

GCGGGGGGACCGTGCTGTTGGCAGAGATATTTTATGGCCTGAGAACGCCCTTTTTTTCTGCCATTGGAAGGATCCAGATTTCTATATATATAAATATCAGGGTGTATATCTGATTTTTGTGGGCCTCTGGTTGGAGCTTTCATGgtgcactttttaacttgtctactgccatgtggaatactcgttcaatttgatatttt
gcaggctgagaataagaacccctcaaggatgaaaaataatgttataaaacaatctgattgcgctttttttttctgagaggctgattagtttctgcaacaatgtctactgtttcttttagtagttctcagacatataggctttatgattcttctgaaagtgatgaagaagggtcttgcagTGATGGTGGTGATAATACAGG
ATATGAATATATGAGCAGCAAATGCTCGCGGGGTTCTATTGATTTCGATCTTGAAAGTGAGGCCGCCATGTCTGAGAATGATGAATGTATGGATGCTAAAATTTCTTCATCCTTCAGATTCATGGCAGGTGCAGCAGATCGCCAAGCTCAACAAGCGATTCATCGCCAACCCCAACAACATGCCAACATGGTTTGGGCAG
ATCCTACAATATCAGAAAGTTCTTGGACTGATGAAAAACATAGCCTGTATCTGAATTCGATAGAGGAGGCCTTTGTTAAGAGCCTCCATGAACGGGGGGGACATTATACATATTCCAGTAAAAGATGGGAGGCTGAGAGGATAACTCCCTGTGTGTCTAGCAAGGGCTTATACGAGGATGTTCATTCTGGGGTTCCATGT
GATTACCGGCCATTTCTATTTCCTGAGGAGAATGGGGCTGAAAGTCAAAGGCGTTGTAGCCATGAAGATATTCAAAATAGAACAAAACGATCGAGATTGGAGGATATATGCATCAAACAATTAGGCCTGCAAGAAAAATATATGAATGGGAAAGCAGATAACTGCCAATCTGAGAAGGAACCAGTTGGAGAAGAAACCAC
AGAAAGAGGAACTATTCAAATGCAATGTTTTAAAGATACTTCTAAGCATGATCATGGAGGCCAAGACCCAGAAACCTCAGAACGGTACATGCAGGAGGATCAGGTTGTTCCATCCACGGAGAATACTTCTAAGAGCTCTCCATAGACAGCAGGATTATCTTCTCTTCTTTTCTATTGTCACCCCAAAAGGAAACTGAAAT
TGTCAGGATGAAAATGGTAGAAATCTGTAATAGACACAGAATGTAATGTTGTAGGCCGAGCTATTCAGAGGAACCAGGATTGTCTTCTTTTCTATGGTCACCAAAAAGGTATGATGAAAATGCTGGAGCCTATAACAAAAGTCTGAACAATTTTGGCTTATTGTATATAAGGGCTCTTGGACATCAGTTTTGTTTTTCTT
GGTAAACCAGCCTGATCAACTCTTTGAAGTTCTTGAATGAATGTCAATGGAAGTTACAAGGAAACGCTTAGTATTGAATGTAAATGGAAGTTACAATGAAATACTTATTATTGACAATAATGAATGTCAATGAAAGTTATTATATTATCTAGCCCTTGGATATTAATTTTGTTTTTCTTCGTAAACAAGCTTATCAACTC
TTTGAAGTTCATGAATGAAT