sp|O48844|PSD1A_ARATH ----> 26S proteasome non-ATPase regulatory subunit 1 homolog A

Intron 1 GTAAGCATCT ... TTTTTTACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	48.57	18	22	12
A2	41.94	9	6	7
A3	35.85	17	7	12
A4	48.48	17	19	13
A5	56.52	5	6	7
A6	43.94	37	29	29
A7	38.46	16	12	8
A8	48.39	8	11	4
A9	41.38	17	8	16
A10	39.53	26	19	15

>TRINITY_DN48907_c0_g1_i4 from sample A7 / Example spliced isoform

AAGAGGCATCCGGTGGAGGAGTTAGTCGAGGAATAGAAGTGAAGTTTTCCGGAATGAGGAACGCCACTTGGTGCAAGCTTCCACCTGTCTGTGTAAGAACACAAGAAACAAAGACGCCACTTCTCATCTCAGGTAGGAGGTGGCACTAATAAACCTTCCACCGACTAAAGCCCTCGGGGGTTAAGAATATACGAATGCAT
GAGAAGTAGGGGGAAAAAAGAAAACAAAGTCAGTCTAGGGTTTTAGAAACTCTGTAATGAGTTCTTTTGATTTATGATGCTCAACGATCCTCAGGGAAGGCACGGCCCCTTGAATGCGCAGCTTTGCATCGGGTTTTCTTTTGGAAATCTCAAGGAATGAGAGTCTCTGGAAAAGAAAGTCTCAAGGCGATCTGTAGGTC
TGATCTCAAGTGGTCAACATGAAGGGTTGAAATCCGAAATCCCATTCTGAATGGCTGAGCAGAGCCACAAACTGGGAAAAGTTCAGTGCTACAGCTGGCTTGGGAGTCATTCACAGGGGTCATTCACGGGGATCATCTTCAACAAGGTCGTTCTTTGATGGCTCCTTACCTTGCATAGAATGGTGCTAGTGGAGGTGGAA
GTCCCTTTTCTGAGGGTGGATCCCTATATTCCTTGGGACTTATACATTCAAACCATGGAGGAGGGATCAAGCAGTTCCTTCATGAAATCTTAAGCAGCGGAAATCTTGAAGGGTTTGGATCTTGGGATAGTCCTTATTGTTTATGGAAGGGAAGAAGAGGCAGATACACTTATTGAACAAATGACAAGGGATCAGGATCC
TATTTTGCGATATGGTGGAATGTATGCACTCGCCTTGGCGTACGGTGGAACAACCAATAAGAAGGCTATCCAATTCCTTCTACACTTTGTTGTCTCAGATGTCAGCGATGATGTGCGAAGAACTGCAGTGTTGGACCTTGAGTTTGTTTTATACTAAGAACCTGAGCAGACACCACGGGTTATATCTTTGCTTTCTGAAT
CACACAATCCACATGTTAGGTATGGAGAAACTATAGCAGTTGGTGTTTCTTGTGCTGGTACATGATTGAGTGAAGCCATTTCACTGCTAGAGCCTCTGACATTAGATGTAGTTGATTTTGTTCGTCAAGGAGCTCTTATAGCAATGGCTATGGTTCTTGTTCAAACAAATGAATCACATGAACCACGAGTAGGTAACTTC
AGGCGTCAATTGGAGAAGATAATTCTTGATAAGCACGAGAACACCATGAGCAACATGGGTGCAATTATGGCTTCTTGTATTTTAGATGCAGAGGTAGAAATGTAAATATTAAAAATCTTTCCAGGTCAAAACATGATAGAATGACTGTTGTTATTGGAATGGCAGTTTTCACACAATTTTGGTATTGAAATTTGGTATCC
ACTTATCTATTTTTTGAGTCCGTCTTCGCACCAACTGCTTTTATTGGGCTTAAATATGACTTGAAGATCCCAAAGTTTGAATTTATATCTGATGCAAAACCTTCATTGTTTGACTATCCCCGCCCAATGGCACCTCCCGCAACTACCTCAGCTGCAAAGCTACCAACAACTGTTTTGTCAACACCAACAAGGGATAAAAC
CAGAGGAAAAAAAGAAG

>TRINITY_DN48907_c0_g1_i1 from sample A7 / Example unspliced isoform:

AAGAGGCATCCGGTGGAGGAGTTAGTCGAGGAATAGAAGTGAAGTTTTCCGGAATGAGGAACGCCACTTGGTGCAAGCTTCCACCTGTCTGTGTAAGAACACAAGAAACAAAGACGCCACTTCTCATCTCAGGTAGGAGGTGGCACTAATAAACCTTCCACCGACTAAAGCCCTCGGGGGTTAAGAATATACGAATGCAT
GAGAAGTAGGGGGAAAAAAGAAAACAAAGTCAGTCTAGGGTTTTAGAAACTCTGTAATGAGTTCTTTTGATTTATGATGCTCAACGATCCTCAGGGAAGGCACGGCCCCTTGAATGCGCAGCTTTGCATCGGGTTTTCTTTTGGAAATCTCAAGGAAGAAAAGTCTCTGGAAAAGAAAGTCTCAAGGCGATCTGTAGGTC
TGATCTCAAGTGGTCAACATGAAGGGTTGAAATCCGAAATCCCATTCTGAATGGCTGAGCAGAGCCACAAACTGGGAAAAGTTCAGTGCTACAGCTGGCTTGGGAGTCATTCACAGGGGTCATTCACGGGGATCATCTTCAACAAGGTCGTTCTTTGATGGCTCCTTACCTTGCATAGAATGGTGCTAGTGGAGGTGGAA
GTCCCTTTTCTGAGGGTGGATCCCTATATTCCTTGGGACTTATACATTCAAACCATGGAGGAGGGATCAAGCAGTTCCTTCATGAAATCTTAAGCAGCGGAAATCTTGAAgtaagcatctgctttggttacatatatgcactattttataataaaaattttgattcaccctatttccgcagtttgttttactctcaaagg
gagcccttacagatgtgatgtattttttacagGGGTTTGGATCTTGGGATAGTCCTTATTGTTTATGGAAGGGAAGAAGAGGCAGATACACTTATTGAACAAATGACAAGGGATCAGGATCCTATTTTGCGATATGGTGGAATGTATGCACTCGCCTTGGCGTACGGTGGAACAACCAATAAGAAGGCTATCCAATTCCT
TCTACACTTTGTTGTCTCAGATGTCAGCGATGATGTGCGAAGAACTGCAGTGTTGGACCTTGAGTTTGTTTTATACTAAGAACCTGAGCAGACACCACGGGTTATATCTTTGCTTTCTGAATCACACAATCCACATGTTAGGTATGGAGAAACTATAGCAGTTGGTGTTTCTTGTGCTGGTACATGATTGAGTGAAGCCA
TTTCACTGCTAGAGCCTCTGACATTAGATGTAGTTGATTTTGTTCGTCAAGGAGCTCTTATAGCAATGGCTATGGTTCTTGTTCAAACAAATGAATCACATGAACCACGAGTAGGTAACTTCAGGCGTCAATTGGAGAAGATAATTATTGATAAGTACGAGAACACCCTGAGTAACATGGGTGCAATTATGGCTTCTTGT
ATTTTAGATGCAGGAGGTAGAAATGTAACCAAGTTCTGTTTAAGTAGAAAGGAGAAATTTGGCCACTGGACATATATAAATGTAGAAAACTAATTTGATCCACTATGTACTACCCATTATTTGTGCATGATTTAGATAGCAAGTCTTATGTGTAACAATCACTTTGAGGAAGGAAGCCACTAATATGTCCTATTTGCATG
ACATGAAAACTTGATTAAGATCCTATTACATCTCTAATCAAGGAGTAGCAATACCTATGTTCTCACGCAAGAAAAGATAAAATGTTTCCTAGTCTCAATCTCCATCAAACATATCTTATGTATTTTCCTATCATGAGCA

Intron 2 GTATGTTCTC ... ATTGTTTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	29.18	85	24.3	44
A2	22.13	64.7	26.3	10
A3	24.12	51.7	22	10.3
A4	19.84	59.7	23.7	5.7
A5	17.47	35	10.7	4
A6	10.85	127	23.3	7.3
A7	19.87	96.3	34.7	12.7
A8	14.79	121.7	25	17.3
A9	13.6	99.7	22	9.3
A10	16.34	62.3	17.3	7

>TRINITY_DN46452_c1_g1_i2 from sample A8 / Example spliced isoform

ATGTTAGGTATGGAGAAACTATAGCAGTTGGTGTTTCGTGTGCTGGTACATGATTGAGTGAAGCCATTTCACTGCTACAGCCTCTGACGTTAGATGTAGTTGATTTTGTTCGTCAAGGAGCTCTTATAGCAATGGCTATGGTTCTTGTTCAAACAAATGAATCACATGAACCACGAGTAGGTAACTTCAGGCGTCAATTG
GAGAAGATAATTATTGATAAGTACGAGAACACCATGATTAACATGGGTGCAATTATGGCTTCTTGTATTTTAGATGCAGGAGGTAGAAATGTAACCAAGTTCTGTTTAAGTAGAAAGGAGAAATTTGGCCACTGGACATATATAAATGTAGAAAACTAATTTGATCCACTATGTACTACCCATTATTTGTGCATGATTTA
GATAGCAAGTCTTATGTGTAACAATCACTTTGAGGAAGGAAGCCACTAATATGTCCTATTTGCATGACATGAAAACTTGATTAAGATCCTATTACATCTCTAATCAAGGAGTAGCAATACCTATGTTCTCACGCAAGAAAAGATAAAATGTTTCCTAGTCTCAATCTCCATCAAACATATCTTATGTATTTTCCTATCAT
GAGG

>TRINITY_DN46452_c1_g1_i4 from sample A8 / Example unspliced isoform:

ATGTTAGGTATGGAGAAACTATAGCAGTTGGTGTTTCGTGTGCTGGTACATGATTGAGTGAAGCCATTTCACTGCTACAGCCTCTGACGTTAGATGTAGTTGATTTTGTTCGTCAAGGAGCTCTTATAGCAATGGCTATGGTTCTTGTTTTTAAACAAATGAATCACATGAACCACGAGTAGGTAACTTCAGgtatgttc
tcccttggactttacaatggctaatgagtactacatagaacttcaaattttaaaagcaaatagtccaacagttcaccaaattgttttcaaaatgttggtctataccatagtgcaattttcactctgttcaaatgcttcatgccattgtttcagGCGTCAATTGGAGAAGATAATTATTGATAAGTACGAGAACACCATGA
TTAACATGGGTGCAATTATGGCTTCTTGTATTTTAGATGCAGGAGGTAGAAATGTAACCAAGTTCTGTTTAAGTAGAAAGGAGAAATTTGGCCACTGGACATATATAAATGTAGAAAACTAATTTGATCCACTATGTACTACCCATTATTTGTGCATGATTTAGATAGCAAGTCTTATGTGTAACAATCACTTTGAGGAA
GGAAGCCACTAATATGTCCTATTTGCATGACATGAAAACTTGATTAAGATCCTATTACATCTCTAATCAAGGAGTAGCAATACCTATGTTCTCACGCAAGAAAAGATAAAATGTTTCCTAGTCTCAATCTCCATCAAACATATCTTATGTATTTTCCTATCATGAGG