sp|P51566|AFC1_ARATH ----> Serine/threonine-protein kinase AFC1

Intron 1 GTAGATTGCT ... TGTTCAAAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	37.81	38.5	23.5	22
A2	35.25	44.5	32.5	16
A3	30.13	30.5	15	11
A4	37.18	33.5	18.5	21
A5	40.16	23.5	13.5	18
A6	44.45	12	9	10
A7	39.93	27.5	15.5	21
A8	40.31	13	5.5	12
A9	33.47	22	11	11
A10	33.85	21.5	9	13

>TRINITY_DN45509_c0_g2_i3 from sample A8 / Example spliced isoform

CCCAAGCTTATATGATTTCCTACGGAAAAACAATTACAGCCCATTTCCCATTGATCTTGTCCGGGAGGTTGGAAGGCAACTGTTGGAATCTGTAGCATATATGCATGAGTTGAAACTCGTTCACACGGATTTAAAGCCTGAAAATATACTTTTTGTTTCTTCAGACTATGTGAAGGTTCCAGATTGCAAGGGTGTGAAGG
GATCTCCAGATGACTTTAAAAGGTTGCCAAAGTCAAGTGCAATAAAGTTAATTGATTTTGGAAGCACCACATATGAAGATCAAGATCACACCTCAGTGGTCTCAACAAGGCATTATCGGGCCCCTGAAGTTATTTTGGGTCTTGGATGGAACTACCCTTGTGATATTTGGAGTGTAGGGTGCATACTTGTGGAGCTCTGC
TCGGTAAACTTTTTAGACTAGTTTATCTTATGTTAAAATTTTACATAGATACCTGCAGGAATGATATTTATGCTACCTGCATTTTAAATTTTCAGGGTGAGACTTTGTTTCAAACCCATGAGAACTTGGAACATCTTGCCATGATGGAAAGGGTCTTAGGGCCTTTGCCACATCAT

>TRINITY_DN45509_c0_g2_i1 from sample A8 / Example unspliced isoform:

CCCAAGCTTATATGATTTCCTACGGAAAAACAATTACAGCCCATTTCCCATTGATCTTGTCCGGGAGGTTGGAAGGCAACTGTTGGAATCTGTAGCATATATGCATGAGTTGAAACTCGTTCACACGGATTTAAAGCCTGAAAATATACTTTTTGTTTCTTCAGACTATGCGAAGGTTCCAGATTGCAAGgtagattgct
cgcgtgttgttactgaatataattgaatttgtcatggttggcatattatatgttgccgacatggctcaactcagttgataatattgtttactattatagtagctccagcaaaaagctggtgtatgaaataacatattgtttctttgtatttttatgccttctactgttaacaatcgttacgatcaaacattttaacatat
tgctaagatcaacattttaacatgcatttatctcaatgaatgaactctgataacattattgttgttcaaaagGGTGTGAAGGGATCTCCAGATGACTTTAAAAGGTTGCCAAAGTCAAGTGCAATAAAGTTAATTGATTTTGGAAGCACCACATATGAAGATCAAGATCACACCTCAGTGGTCTCAACAAGGCATTATCG
GGCCCCTGAAGTTATTTTGGGTGAGCTTAGAAAGATTGTTCTATGGTTTAGCCAGCAGTTTGAAAGGACCAATGTCAATTCAATCATATGTTGAATCCAAACCCCTTTGTATTTACAATTTATGTTTTACAACAGGTCTTGGATGGAACTACCCTTGTGATATTTGGAGTGTAGGGTGCATACTTGTGGAGCTCTGCTCG
GTAAACTTTTTAGACTAGTTTATCTTATGTTAAAATTTTACATAGATACCTGCAGGAATGATATTTATGCTACCTGCATTTTAAATTTTCAGGGTGAGACTTTGTTTCAAACCCATGAGAACTTGGAACATCTTGCCATGATGGAAAGGGTCTTAGGGCCTTTGCCACATCAT

Intron 2 GTGAGCTTAG ... TTTACAACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	47.76	35	33	31
A2	34.33	22	13	10
A3	34.25	24	16	9
A4	59.26	11	18	14
A5	66.1	10	22	17
A6	33.33	23	15	8
A7	48.24	22	30	11
A8	48.72	20	22	16
A9	44	14	15	7
A10	49.02	13	13	12

>TRINITY_DN45509_c0_g2_i3 from sample A8 / Example spliced isoform

CCCAAGCTTATATGATTTCCTACGGAAAAACAATTACAGCCCATTTCCCATTGATCTTGTCCGGGAGGTTGGAAGGCAACTGTTGGAATCTGTAGCATATATGCATGAGTTGAAACTCGTTCACACGGATTTAAAGCCTGAAAATATACTTTTTGTTTCTTCAGACTATGTGAAGGTTCCAGATTGCAAGGGTGTGAAGG
GATCTCCAGATGACTTTAAAAGGTTGCCAAAGTCAAGTGCAATAAAGTTAATTGATTTTGGAAGCACCACATATGAAGATCAAGATCACACCTCAGTGGTCTCAACAAGGCATTATCGGGCCCCTGAAGTTATTTTGGGTCTTGGATGGAACTACCCTTGTGATATTTGGAGTGTAGGGTGCATACTTGTGGAGCTCTGC
TCGGTAAACTTTTTAGACTAGTTTATCTTATGTTAAAATTTTACATAGATACCTGCAGGAATGATATTTATGCTACCTGCATTTTAAATTTTCAGGGTGAGACTTTGTTTCAAACCCATGAGAACTTGGAACATCTTGCCATGATGGAAAGGGTCTTAGGGCCTTTGCCACATCAT

>TRINITY_DN45509_c0_g2_i2 from sample A8 / Example unspliced isoform:

CCCAAGCTTATATGATTTCCTACGGAAAAACAATTACAGCCCATTTCCCATTGATCTTGTCCGGGAGGTTGGAAGGCAACTGTTGGAATCTGTAGCATGTGTGTAGACGATACTTGCACTTATCTTTACATTTGATGTGGGAGGGCTTAAACATATAATTTAATTTTTTAAGGATTGCTTGCCCACTGCCATAACTAGAT
TGCTTCGTTGGTCATGTTAGCTCACATTTTATGAGGCTAGATTGGGCCTTTATAAGGGTGTTCATAACATCTGTCTAGGATTTTCTTCTCCTAATATTGCCAATGATCTGTGGCAGATATGCATGAGTTGAAACTCGTTCACACGGATTTAAAGCCTGAAAATATACTTTTTGTTTCTTCAGACTATGCGAAGGTTCCAG
ATTGCAAGGTAGATTGCTCGCGTGTTGTTACTGAATATAATTGAATTTGTCATGGTTGGCATATTATATGTTGCCGACATGGCTCAACTCAGTTGATAATATTGTTTACTATTATAGTAGCTCCAGCAAAAAGCTGGTGTATGAAATAACATATTGTTTCTTTGTATTTTTATGCCTTCTACTGTTAACAATCGTTACGA
TCAAACATTTTAACATATTGCTAAGATCAACATTTTAACATGCATTTATCTCAATGAATGAACTCTGATAACATTATTGTTGTTCAAAAGGGTGTGAAGGGATCTCCAGATGACTTTAAAAGGTTGCCAAAGTCAAGTGCAATAAAGTTAATTGATTTTGGAAGCACCACATATGAAGATCAAGATCACACCTCAGTGGT
CTCAACAAGGCATTATCGGGCCCCTGAAGTTATTTTGGgtgagcttagaaagattgttctatggtttagccagcagtttgaaaggaccaatgtcaattcaatcatatgttgaatccaaacccctttgtatttacaatttatgttttacaacagGTCTTGGATGGAACTACCCTTGTGATATTTGGAGTGTAGGGTGCATA
CTTGTGGAGCTCTGCTCGGTAAACTTTTTAGACTAGTTTATCTTATGTTAAAATTTTACATAGATACCTGCAGGAATGATATTTATGCTACCTGCATTTTAAATTTTCAGGGTGAGACTTTGTTTCAAACCCATGAGAACTTGGAACATCTTGCCATGATGGAAAGGGTCTTAGGGCCTTTGCCACATCAT

Intron 3 GTGTGTAGAC ... TCTGTGGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	61.63	21.3	22	46.3
A2	49.24	23	16	28.7
A3	60.2	18	27	27.3
A4	59.92	19	25	31.3
A5	52.02	11	11	12.7
A6	41.42	11.7	8	8.3
A7	39.7	21	9	18.7
A8	48.6	5.3	3	7
A9	58.95	8	5	17.7
A10	62.02	7.7	12	13

>TRINITY_DN45509_c0_g2_i3 from sample A8 / Example spliced isoform

CCCAAGCTTATATGATTTCCTACGGAAAAACAATTACAGCCCATTTCCCATTGATCTTGTCCGGGAGGTTGGAAGGCAACTGTTGGAATCTGTAGCATATATGCATGAGTTGAAACTCGTTCACACGGATTTAAAGCCTGAAAATATACTTTTTGTTTCTTCAGACTATGTGAAGGTTCCAGATTGCAAGGGTGTGAAGG
GATCTCCAGATGACTTTAAAAGGTTGCCAAAGTCAAGTGCAATAAAGTTAATTGATTTTGGAAGCACCACATATGAAGATCAAGATCACACCTCAGTGGTCTCAACAAGGCATTATCGGGCCCCTGAAGTTATTTTGGGTCTTGGATGGAACTACCCTTGTGATATTTGGAGTGTAGGGTGCATACTTGTGGAGCTCTGC
TCGGTAAACTTTTTAGACTAGTTTATCTTATGTTAAAATTTTACATAGATACCTGCAGGAATGATATTTATGCTACCTGCATTTTAAATTTTCAGGGTGAGACTTTGTTTCAAACCCATGAGAACTTGGAACATCTTGCCATGATGGAAAGGGTCTTAGGGCCTTTGCCACATCAT

>TRINITY_DN45509_c0_g2_i2 from sample A8 / Example unspliced isoform:

CCCAAGCTTATATGATTTCCTACGGAAAAACAATTACAGCCCATTTCCCATTGATCTTGTCCGGGAGGTTGGAAGGCAACTGTTGGAATCTGTAGCATgtgtgtagacgatacttgcacttatctttacatttgatgtgggagggcttaaacatataatttaattttttaaggattgcttgcccactgccataactagat
tgcttcgttggtcatgttagctcacattttatgaggctagattgggcctttataagggtgttcataacatctgtctaggattttcttctcctaatattgccaatgatctgtggcagATATGCATGAGTTGAAACTCGTTCACACGGATTTAAAGCCTGAAAATATACTTTTTGTTTCTTCAGACTATGCGAAGGTTCCAG
ATTGCAAGGTAGATTGCTCGCGTGTTGTTACTGAATATAATTGAATTTGTCATGGTTGGCATATTATATGTTGCCGACATGGCTCAACTCAGTTGATAATATTGTTTACTATTATAGTAGCTCCAGCAAAAAGCTGGTGTATGAAATAACATATTGTTTCTTTGTATTTTTATGCCTTCTACTGTTAACAATCGTTACGA
TCAAACATTTTAACATATTGCTAAGATCAACATTTTAACATGCATTTATCTCAATGAATGAACTCTGATAACATTATTGTTGTTCAAAAGGGTGTGAAGGGATCTCCAGATGACTTTAAAAGGTTGCCAAAGTCAAGTGCAATAAAGTTAATTGATTTTGGAAGCACCACATATGAAGATCAAGATCACACCTCAGTGGT
CTCAACAAGGCATTATCGGGCCCCTGAAGTTATTTTGGGTGAGCTTAGAAAGATTGTTCTATGGTTTAGCCAGCAGTTTGAAAGGACCAATGTCAATTCAATCATATGTTGAATCCAAACCCCTTTGTATTTACAATTTATGTTTTACAACAGGTCTTGGATGGAACTACCCTTGTGATATTTGGAGTGTAGGGTGCATA
CTTGTGGAGCTCTGCTCGGTAAACTTTTTAGACTAGTTTATCTTATGTTAAAATTTTACATAGATACCTGCAGGAATGATATTTATGCTACCTGCATTTTAAATTTTCAGGGTGAGACTTTGTTTCAAACCCATGAGAACTTGGAACATCTTGCCATGATGGAAAGGGTCTTAGGGCCTTTGCCACATCAT