sp|P05621|H2B2_WHEAT ----> Histone H2B.2

Intron 1 GTATCTTTTT ... AATATTTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	44.95	60	13	49
A2	32.64	97	2	47
A3	37.5	90	31	54
A4	41.44	65	22	46
A5	51.69	43	15	46
A6	42.09	743	151	540
A7	39.4	646	146	420
A8	40.99	537	114	373
A9	39.75	667	151	440
A10	42.31	439	119	322

>TRINITY_DN111388_c0_g1_i2 from sample A5 / Example spliced isoform

CACTTGTCCATCCTCTAAACTGTCAAGCTTAATTTTTCTTTGCCCGCTGCAGTTTTGATAATACTTTTGTATTGAAGGAAAAGTATTATCAGACCTTTGCAGATAATCATTCCACCTTCAGTTATGGCTATTGTGAAAAGAGGAACCCCTGAATGCTCTGGTTCCTGGCAACTAAGAGAGGGCTAACAAAGGCAGGGACT
AGCTGCTGTCGGAGATATCATCTTTGCCGTCGTCGTCAAGCGTGCCCAGGAAGAAGCGAACAAAGAAAGTGATACAGACATTCAATATCTACATATTCAAGGTCTTGAAGATGATCCATCCCGACAGCGGAATTTCTTCTAAGGCAACATCATGAATTCTTTCCTCAATGACATCTTTGATAAGCTCGCAACGGAGGCCT
GTCGTCTCGCGATTTGTAACCGGAAGCCCACCGCTACTTCCAGGGAGATTCAGACTGCAGCCAGGCTTATTTCTCCAGGGGAGTTGGCCAAGCATGCGGTATCTGAGGGCAACAGGGCTGTTATTAAGTTTACTAGCCCTAAGTAGCCAGCCAGCCAGCCATGGATGATGGCCTTTTGAGTTTGATTTTTGAATCGATTT
TTAAGTTTTTTTCATTTTTTACAAAGATACATATATCTGATCTTCAGAGTTTTCAAAGATTTGCCCGAGGTTTTTTGAAATTGTGTTCTGGTTGTTTTAATGATGGAAAAGATTTCTTTAAGAGGGTCAGTAGCCAGGCAAGATATTTTGAGAGCTTGAGTATATATGGCGCAAATCTCAATGATACAAATGCAAGTTCC
AGACAATTCATGAAGTATTTCTCACTTCTGTCTTTGAATCTTTTGAGTTTGCTTTTTGAAGCGATTTGTATGTTCTTTTCATTTTCTTACAAAGTTGCATTATCTGATCTTCAGAGTTTTCAAAGATTTGCAGAGGTTTTTTGAAATTGTGTTCT

>TRINITY_DN111388_c0_g1_i10 from sample A5 / Example unspliced isoform:

CACTTGTCCATCCTCTAAACTGTCAAGCTTAATTTTTCTTTGCCCGCTGCAGTTTTGATAATACTTTTGTATTGAAGGAAAAGTATTATCAGACCTTTGCAGATAATCATTCCACCTTCAGTTATGGCTATTGTGAAAAGAGGAACCCCTGAATGCTCTGgtatctttttgtctgctcaattttggaggtaaatggactt
tttccatgaattttccttccttgcgttttctaaatttgatggaaaatatttcagGTTCCTGGCAACTAAGAGAGGGCTAACAAAGGCAGGGACTAGCTGCTGTCGGAGATATCATCTTTGCCGTCGTCGTCAAGCGTGCCCAGGAAGAAGCGAACAAAGAAAGTGATACAGACATTCAATATCTACATATTCAAGGTCTT
GAAGATGATCCATCCCGACAGCGGAATTTCTTCTAAGGCAACATCATGAATTCTTTCCTCAATGACATCTTTGATAAGCTCGCAACGGAGGCCTGTCGTCTCGCGATTTGTAACCGGAAGCCCACCGCTACTTCCAGGGAGATTCAGACTGCAGCCAGGCTTATTTCTCCAGGGGAGTTGGCCAAGCATGCGGTATCTGA
GGGCAACAGGGCTGTTATTAAGTTTACTAGCCCTAAGTAGCCAGCCAGCCAGCCATGGATGATGGCCTTTTGAGTTTGATTTTTGAATCGATTTTTAAGTTTTTTTCATTTTTTACAAAGATACATATATCTGATCTTCAGAGTTTTCAAAGATTTGCCCGAGGTTTTTTGAAATTGTGTTCTGGTTGTTTTAATGATGG
AAAAGATTTCTTTAAGAGGGTCAGTAGCCAGGCAAGATATTTTGAGAGCTTGAGTATATATGGCGCAAATCTCAATGATACAAATGCAAGTTCCAGACAATTCATGAAGTATTTCTCACTTCTGTCTTTGAATCTTTTGAGTTTGCTTTTTGAAGCGATTTGTATGTTCTTTTCATTTTCTTACAAAGTTGCATTATCTG
ATCTTCAGAGTTTTCAAAGATTTGCAGAGGTTTTTTGAAATTGTGTTCT

Intron 2 GTAAATGGAC ... ACAAAGGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	100	0	17	605
A2	100	0	5	636
A3	99.88	1	34	822
A4	100	0	33	554
A5	99.53	2	18	421
A6	99.88	7	182	5711
A7	99.97	2	158	6303
A8	99.92	4	138	5248
A9	99.95	3	171	5979
A10	99.98	1	139	4967

>TRINITY_DN111388_c0_g1_i7 from sample A5 / Example spliced isoform

CACTTGTCCATCCTCTAAACTGTCAAGCTTAATTTTTCTTTGCCCGCTGCAGTTTTGATAATACTTTTGTATTGAAGGAAAAGTATTATCAGACCTTTGCAGATAATCATTCCACCTTCAGTTATGGCTATTGTGAAAAGAGGAACCCCTGAATGCTCTGGTATCTTTTTGTCTGCTCAATTTTGGAGGGACTAGCTGCT
GTCGGAGATATCATCTTTGCCGTCGTCGTCAA

>TRINITY_DN111388_c0_g1_i10 from sample A5 / Example unspliced isoform:

CACTTGTCCATCCTCTAAACTGTCAAGCTTAATTTTTCTTTGCCCGCTGCAGTTTTGATAATACTTTTGTATTGAAGGAAAAGTATTATCAGACCTTTGCAGATAATCATTCCACCTTCAGTTATGGCTATTGTGAAAAGAGGAACCCCTGAATGCTCTGGTATCTTTTTGTCTGCTCAATTTTGGAGgtaaatggactt
tttccatgaattttccttccttgcgttttctaaatttgatggaaaatatttcaggttcctggcaactaagagagggctaacaaaggcagGGACTAGCTGCTGTCGGAGATATCATCTTTGCCGTCGTCGTCAAGCGTGCCCAGGAAGAAGCGAACAAAGAAAGTGATACAGACATTCAATATCTACATATTCAAGGTCTT
GAAGATGATCCATCCCGACAGCGGAATTTCTTCTAAGGCAACATCATGAATTCTTTCCTCAATGACATCTTTGATAAGCTCGCAACGGAGGCCTGTCGTCTCGCGATTTGTAACCGGAAGCCCACCGCTACTTCCAGGGAGATTCAGACTGCAGCCAGGCTTATTTCTCCAGGGGAGTTGGCCAAGCATGCGGTATCTGA
GGGCAACAGGGCTGTTATTAAGTTTACTAGCCCTAAGTAGCCAGCCAGCCAGCCATGGATGATGGCCTTTTGAGTTTGATTTTTGAATCGATTTTTAAGTTTTTTTCATTTTTTACAAAGATACATATATCTGATCTTCAGAGTTTTCAAAGATTTGCCCGAGGTTTTTTGAAATTGTGTTCTGGTTGTTTTAATGATGG
AAAAGATTTCTTTAAGAGGGTCAGTAGCCAGGCAAGATATTTTGAGAGCTTGAGTATATATGGCGCAAATCTCAATGATACAAATGCAAGTTCCAGACAATTCATGAAGTATTTCTCACTTCTGTCTTTGAATCTTTTGAGTTTGCTTTTTGAAGCGATTTGTATGTTCTTTTCATTTTCTTACAAAGTTGCATTATCTG
ATCTTCAGAGTTTTCAAAGATTTGCAGAGGTTTTTTGAAATTGTGTTCT