sp|Q9LVR5|MCU3_ARATH ----> Calcium uniporter protein 3, mitochondrial

Intron 1 GTATGGTTTG ... ATGGGTGTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	51.72	28	8	30
A2	38.46	16	2	10
A3	76.19	5	5	16
A4	48	13	7	12
A5	75	2	5	6
A6	36.36	63	3	36
A7	31.23	185	6	84
A8	32.96	181	5	89
A9	30.43	144	4	63
A10	32.84	135	1	66

>TRINITY_DN44271_c0_g1_i4 from sample A6 / Example spliced isoform

AAAGAGCTTCGCATGATTGCAGTCTGCTTGTGGCCGCCTATTAGATGCGATTTAGGCCGACGGCGTTCAATTCTTCCTTGTCACTATTCCAGACTTCAAATGTTCATGCGGATCTCTGAACAATTGAAGATATCGGGATCGCGTGGAGATCATCAGTCTAAAGAAGACTCATGCCAACTGGAGAGATCACTCATGCCAAC
GACGAGGAGATCATAGCCGTGTCCACTAACGAAGAGGCGATCAAAGAAAGGAAAGAAATCAGTTATGGTTTCATGATCTCTGACTTCCCCTATACTGTTAATCAAAGAAATGAAAGAAATCAGTATATCAATCTAGACTTTTGATAGTTAGAAATTTACAGGAAAAGACATATTATGAATGCATTCGTAAATTTTATGTC
TATTAAGCCTGTTATCAATGAAGTTGACCAGGAATACCCGCTGCCT

>TRINITY_DN44271_c0_g1_i8 from sample A6 / Example unspliced isoform:

TTCAATTCTTCCTTGTCACTATTCCAGACTTCAAATGTTCATGCGGATCTCTGAACAATTGAAGATATCGGGATCGCGTGGAGATCATCAGgtatggtttggcctatcctgttaatgaaagaaagcagtatatcaatatagacttgatagttataaatattgaacagtaaaagacatagatcgaaggagttattggttca
agattcacttgactgaattcttattttaatttgttctgcaatgggtgtagTCTAAAGAAGACTCATGCCAACTGGAGAGATCACTCATGCCAACGACGAGGAGATCATAGCCGTGTCCACTAACGAAGAGGCGATCAAAGAAAGGAAAGAAATCAGTTATGGTTTCATGATCTCTGACTTCCCCTATACTGTTAATCAAA
GAAATGAAAGAAATCAGTATATCAATCTAGACTTTTGATAGTTAGAAATTTACAGGAAAAGACATATTATGAATGCATTCGTAAATTTTATGTCTATTAAGCCTGTTATCAATGAAGTTGACCAGGAATACCCGCTGCCT

Intron 2 GTAATGATCA ... GCATCCTTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	66.2	18.6	31	16.4
A2	76.09	8.6	22.8	5.4
A3	65.09	8.6	14.8	5.8
A4	84.31	5.2	21.8	4.4
A5	76.03	3.4	9.8	6.4
A6	47.18	47.6	34	6
A7	41.3	114.2	52.6	22
A8	36.8	119.4	49.2	12
A9	39.15	100.4	45.8	18
A10	40.34	83.2	37.4	13.2

>TRINITY_DN44271_c0_g1_i8 from sample A6 / Example spliced isoform

TTCAATTCTTCCTTGTCACTATTCCAGACTTCAAATGTTCATGCGGATCTCTGAACAATTGAAGATATCGGGATCGCGTGGAGATCATCAGGTATGGTTTGGCCTATCCTGTTAATGAAAGAAAGCAGTATATCAATATAGACTTGATAGTTATAAATATTGAACAGTAAAAGACATAGATCGAAGGAGTTATTGGTTCA
AGATTCACTTGACTGAATTCTTATTTTAATTTGTTCTGCAATGGGTGTAGTCTAAAGAAGACTCATGCCAACTGGAGAGATCACTCATGCCAACGACGAGGAGATCATAGCCGTGTCCACTAACGAAGAGGCGATCAAAGAAAGGAAAGAAATCAGTTATGGTTTCATGATCTCTGACTTCCCCTATACTGTTAATCAAA
GAAATGAAAGAAATCAGTATATCAATCTAGACTTTTGATAGTTAGAAATTTACAGGAAAAGACATATTATGAATGCATTCGTAAATTTTATGTCTATTAAGCCTGTTATCAATGAAGTTGACCAGGAATACCCGCTGCCT

>TRINITY_DN44271_c0_g1_i2 from sample A6 / Example unspliced isoform:

AAAGAGCTTCGCATGATTGCAGTCTGCTTGTGGCCGCCTATTAGATGCGATTTAGGCCGACGGCGTTCAATTCTTCCTTGTCACTATTCCAGACTTCAAATGTTCATGCGGgtaatgatcaataaatggatagcggattacatttttttccttgccgcaatttttcttgaatacacttcttcctcgccttcatatctttg
aattctgtgcatccttagATCTCTGAACAATTG

Intron 3 GTATGGTTAA ... ATGGGTGTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	50.88	28	25	33
A2	71.17	16	30	49
A3	86.84	5	25	41
A4	53.57	13	11	19
A5	89.19	2	9	24
A6	43.5	63	42	55
A7	35.43	185	80	123
A8	29.57	181	51	101
A9	41.7	144	70	136
A10	35.71	135	62	88

>TRINITY_DN44271_c0_g1_i4 from sample A6 / Example spliced isoform

AAAGAGCTTCGCATGATTGCAGTCTGCTTGTGGCCGCCTATTAGATGCGATTTAGGCCGACGGCGTTCAATTCTTCCTTGTCACTATTCCAGACTTCAAATGTTCATGCGGATCTCTGAACAATTGAAGATATCGGGATCGCGTGGAGATCATCAGTCTAAAGAAGACTCATGCCAACTGGAGAGATCACTCATGCCAAC
GACGAGGAGATCATAGCCGTGTCCACTAACGAAGAGGCGATCAAAGAAAGGAAAGAAATCAGTTATGGTTTCATGATCTCTGACTTCCCCTATACTGTTAATCAAAGAAATGAAAGAAATCAGTATATCAATCTAGACTTTTGATAGTTAGAAATTTACAGGAAAAGACATATTATGAATGCATTCGTAAATTTTATGTC
TATTAAGCCTGTTATCAATGAAGTTGACCAGGAATACCCGCTGCCT

>TRINITY_DN44271_c0_g1_i10 from sample A6 / Example unspliced isoform:

ATCTCTGAACAATTGAAGATATCGGGATCGCGTGGAGATCATCAGgtatggttaatgcaaaagaacggaaagaaataaagagatcaatctagacttgatagttagaaatctacaagaaaatacataattggttcaagattcagttgaccgaattcttattttaatttgttctgcaatgggtgtagTCTAAAGAAGACTCA
TGCCAACTGGAGGAGATCACTCATGCCAACCAACGAAAAGGAGA

Intron 4 GTATGGTTAA ... ATGGGTGTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	15.7	337	78.5	47
A2	21.09	290	96	59
A3	17.86	284	72	51.5
A4	14.44	234	55	24
A5	17.91	149	36	29
A6	11.6	962	183	69.5
A7	12.97	2046	463	147
A8	11.46	2270	455	132.5
A9	11.6	2025	362	169.5
A10	12.39	1650	349	118

>TRINITY_DN44271_c0_g1_i6 from sample A6 / Example spliced isoform

CGACGACCGCCCACCAAGTTGTTAGCACCACGGTCATGGCGGGGGAGCTCTTGGCCTGCGATATCTCTTCCGTAGTGAGATGACTGCATCGACGCATCATGGACAGAGCCGAGATGCAGAGTTATAAAGAAGACCGCACTGTATTCAAGATCTGAGATTACTGCATCGCATCATGGCCGACAAAACTCAGATGGACAGAA
CAGACGACGACGAGGAGATCAAGATCGTGATCGCGTGGAGATAATCAGTCTAAAGAAGACTCATGCCAACTGGAGGAGATCACTCATGCCAACCGACGAAAAGGACATCAAAGAAAGGAAAGAAATTGATAGTTAGAAATCAAAGAAATGAAAGAAATTGATAGTTAGAAATGTACAAAGAAATTGATAGTTTCGTGATC
TATGACTTCCCCTATACTGTTAATCAAAGAAATGAAAGAAATCAGTATATCAATCTAGACTTTTGATAGTTAGAAATTTACAGGAAAAGACATATTATGAATGCATTCGTAAATTTTATGTCTATTAAGCCTGTTATCAATGAAGTTGACCAGGAATACCCGCTGCCT

>TRINITY_DN44271_c0_g1_i3 from sample A6 / Example unspliced isoform:

CGACGACCGCCCACCAAGTTGTTAGCACCACGGTCATGGCGGGGGAGCTCTTGGCCTGCGATATCTCTTCCGTAGTGAGATGACTGCATCGACGCATCATGGACAGAGCCGAGATGCAGAGTTATAAAGAAGACCGCACTGTATTCAAGATCTGAGATTACTGCATCGCATCATGGCCGACAAAACTCAGATGGACAGAA
CAGACGACGACGAGGAGATCAAGATCGTGATCGCGTGGAGATAATCAGgtatggttaatgcaaaagaaataaagagatcaatctagacttgatagttagaaatgtacaagaaaatacataattcgaaggagttattggttcaagattcagttgaccgaattcttattttaatttgttctgcaatgggtgtagTCTAAAGA
AGACTCATGCCAACTGGAGGAGATCACTCATGCCAACCGACGAAAAGGACATCAAAGAAAGGAAAGAAATTGATAGTTAGAAATCAAAGAAATGAAAGAAATTGATAGTTAGAAATGTACAAAGAAATTGATAGTTTCGTGATCTATGACTTCCCCTATACTGTTAATCAAAGAAATGAAAGAAATCAGTATATCAATCT
AGACTTTTGATAGTTAGAAATTTACAGGAAAAGACATATTATGAATGCATTCGTAAATTTTATGTCTATTAAGCCTGTTATCAATGAAGTTGACCAGGAATACCCGCTGCCT