sp|O24371|LOX31_SOLTU ----> Linoleate 13S-lipoxygenase 3-1, chloroplastic

Intron 1 GTATTTTAAT ... GATTTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	28.57	15	6	3
A2	50	7	7	1
A3	38.71	19	12	4
A4	36	16	9	3
A5	36.11	23	13	2
A6	0	5	0	0
A7	36.67	19	11	0
A8	0	1	0	0
A9	42.31	15	11	6
A10	46.88	17	15	4

>TRINITY_DN96745_c0_g1_i1 from sample A4 / Example spliced isoform

TTGAACGGTAGTTTTATCTGTGGGGGCACTTTTCTTTTTTCCCTTTCCCTTCTTCTAATCGATCACGCGCTGTGCATGTTTTTTATTTTTGACGGGTCCCATGAACAATAGATTCTGTCAGCTCATACGAACAATAGAAAAAATCGCAGTATTTTTTACATATAAATATAGATAGATAGATGTGTAGGATGAATCCATCT
TCATAGATGATCTGCTGCAGCATAAACCTGGAGCTTGAGTGCAGGTACAAATAATTTAGTCCATTAGGCTCTTGGCAGGACCAGTGGTGTGCCAGACTCTAGTATTCTAGTGTGCAACAAGGATGCTGATGAGGGTTGCCAATATGGAGCAGGTCCCGTGTTCGATCGCCGATGTCAGATCGTCTATGTTGCTCAATGGA
CACCAGATGCTCAGACGAAGCACAGCCCGCGAGTTTTGCTTCACGAGAAATTTTCCAGGCAGGCAATCGGGCTGCTGCTGGAACGATGGTAATTGCAAAAGGAGAATCGGTCCAATTCGTGCAGTTATCAGCGACAAACCCGCCACGACCACCGGCGTGACGCCTCCTGTTGACAAGGGTGCGATGATCAATGTGAAGGC
CGTGGCCATCATCAGGAAGAAACTCAAGGAGGACATGAAGGAGAAGATCGAGAATCAGTGGGAGTGTCTGTTAGATGTCATTGGCCAGAACGTCCTTCTGGAGCTAGTCAGTGTTGACATCGATCCAGAGACAAGTTGTGGAAAGAGAACCAAAGAATCTGCTTTGAAGGGCTGGCTGCTGCAGACTGCTCGTGAAGACA
ACACAATGGAATGCACTGCAAATTTTACAGTAAGCGCTGACTTTGGTTGTCCGGGTGCTATTCTAGTTACCAATCAACACGACAAAGAAATCTTTTTGGAATCCATTGCTGTGGAAGGGTTCAACAGCGAGCCGATCTACTTCCCTTGCCACTCTTGGGTCCATTCAAAAAAGGATAATCCCGAGAAAAGAATCTTCTTC
AGCAATCAGCCATATTTGCCTTCGAAAACCCCAGATGGGCTAGAGGATATTCGGCAGAAGGACATGAAAGACAAGCGTGGGGATGGAAAAGGCGAACGCAAGCATTGGGATAGAATTTATGACTACGCGACTTACAATGATTTAGGGAACGCTGATAAAAGTGAGGATCTTGCGAGACCGAATCTTTGTGGTGATGATAT
ACCTTATCCAAGACGCTGTCGCACTGGCCGTCCTCCTATGAAGACAGATCCATCTGCAGAAAGCACAAAAGGAAAGCCCGCTACTATATATGTTCCACGGGATGAAACATTTGAAGAGATCAAGAAAAGTACATTTTCTGCAGGGGCTCTAAAAGCATTATTCCATAATTTGGTACCATCTTTGATAGCCAGTTTCTCGA
ATCCCAATTATGAGTTTGGGTGCTTTTCTGATATTGATCGCTTGTATAGAGAGGGTCAGATCTTAACGTTTGAG

>TRINITY_DN96745_c0_g1_i2 from sample A4 / Example unspliced isoform:

TTGAACGGTAGTTTTATCTGTGGGGGCACTTTTCTTTTTTCCCTTTCCCTTCTTCTAATCGATCACGCGCTGTGCATGTTTTTTATTTTTGACGGGTCCCATGAACAATAGATTCTGTCAGCTCATACGAACAATAGAAAAAATCGCAGTATTTTTTACATATAAATATAGATAGATAGATGTGTAGGATGAATCCATCT
TCATAGATGATCTGCTGCAGCATAAACCTGgtattttaatccattttgcagtagttgttatgattttgcagGAGCTTGAGTGCAGGTACT

Intron 2 GTAGTTTTAT ... TTTGCAGTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	61.54	10	1	16
A2	75	3	0	9
A3	78.26	5	0	18
A4	71.43	4	1	10
A5	80	4	8	16
A6	60	4	0	6
A7	78.26	5	0	18
A8	50	1	0	1
A9	78.26	5	1	18
A10	83.33	2	0	10

>TRINITY_DN101120_c0_g1_i2 from sample A5 / Example spliced isoform

ATTGTACACATGCCAAGAGCGAGAGCTCATGGATTTGTATTGAACGGAGCTTGAGTGCAGGTACAAATAATTTAGTCCATTAGGCTCTTGGCAGGACCAGTGGTGTGCCAGACTCTAGTATTCTAGTGTGCAACAAGGATGCTGATGAGGGTTGCCAATATGGAGCAGGTCCCGTGTTCGATCGCCGATGTCAGATCGTC
TATGTTGCTCAATGGACACCAGATGCTCAGACGAAGCACAGCCCGCGAGTTTTGCTTCACGAGAAATTTTCCAGGCAGGCAATCGGGCTGCTGCTGGAACGATGGTAATTGCAAAAGGAGAATCGGTCCAATTCGTGCAGTTATCAGCGACAAACCCGCCACGACCACCGGCGTGACGCCTCCTGTTGACAAGGGTGCGA
TGATCAATGTGAAGGCCGTGGCCATCATCAGGAAGAAACTCAAGGAGGACATGAAGGAGAAGATCGAGAATCAGTGGGAGTGTCTGTGTGATGTCATTGGCCAGAACGTCCTTCTGGAGCTAGTCAGTGTTGACATCGATCCAGAGACAAGTTGTGGCAAGAGAACCAAAGAATCTGCTTTGAAGGGCTGGCTGCTGCAG
ACTGCTCGTGAAGACAACACAATGGAATGCACTGCAAATTTTACAGTAAGCGCTGACTTTGGTTGTCCGGGTGCTATTCTAGTTACCAATCAACACGACAAAGAAATCTTTTTGGAATCCATTGCTGTGGAAGGGTTCAACAGCGAGCCGATCTACTTCCCTTGCCACTCTTGGGTCCATTCAAAAAAGGATAATCCCGA
GAAAAGAATCTTCTTCAGCAATCAGCCATATTTGCCTTCGAAAACCCCAGATGGGCTAGAGGATATTCGGCAGAAGGACATGAAAGACAAGCGGGGG

>TRINITY_DN101120_c0_g1_i1 from sample A5 / Example unspliced isoform:

ATTGTACACATGCCAAGAGCGAGAGCTCATGGATTTGTATTGAACGgtagttttatctgtgggggcacttttcctttttccctgtcccttcttctaatcgatcacgcgctgtgcatgttttttatttttgacgggtcccatgaacaatagattctgtcagctcatacgaacaatagaaaaaatcgcagtattttttacat
ataaatatagatagatagatgtgtaggatgaatccatcttcatagatgatctgctgcagcataaacctggtattttaatccattttgcagtagGAGCTTGAGTGCAGGTACAAATAATTTAGTCCATTAGGCTCTTGGCAGGACCAGTGGTGTGCCAGACTCTAGTATTCTAGTGTGCAACAAGGATGCTGATGAGGGTT
GCCAATATGGAGCAGGTCCCGTGTTCGATCGCCGATGTCAGATCGTCTATGTTGCTCAATGGACACCAGATGCTCAGACGAAGCACAGCCCGCGAGTTTTGCTTCACGAGAAATTTTCCAGGCAGGCAATCGGGCTGCTGCTGGAACGATGGTAATTGCAAAAGGAGAATCGGTCCAATTCGTGCAGTTATCAGCGACAA
ACCCGCCACGACCACCGGCGTGACGCCTCCTGTTGACAAGGGTGCGATGATCAATGTGAAGGCCGTGGCCATCATCAGGAAGAAACTCAAGGAGGACATGAAGGAGAAGATCGAGAATCAGTGGGAGTGTCTGTGTGATGTCATTGGCCAGAACGTCCTTCTGGAGCTAGTCAGTGTTGACATCGATCCAGAGACAAGTT
GTGGCAAGAGAACCAAAGAATCTGCTTTGAAGGGCTGGCTGCTGCAGACTGCTCGTGAAGACAACACAATGGAATGCACTGCAAATTTTACAGTAAGCGCTGACTTTGGTTGTCCGGGTGCTATTCTAGTTACCAATCAACACGACAAAGAAATCTTTTTGGAATCCATTGCTGTGGAAGGGTTCAACAGCGAGCCGATC
TACTTCCCTTGCCACTCTTGGGTCCATTCAAAAAAGGATAATCCCGAGAAAAGAATCTTCTTCAGCAATCAGCCATATTTGCCTTCGAAAACCCCAGATGGGCTAGAGGATATTCGGCAGAAGGACATGAAAGACAAGCGGGGG

Intron 3 GTATTTTAAT ... TTTGCAGTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	49.15	15	13	16
A2	54.84	7	8	9
A3	48.65	19	18	18
A4	40.74	16	12	10
A5	45.24	23	22	16
A6	47.37	5	3	6
A7	50	19	20	18
A8	50	1	1	1
A9	53.12	15	16	18
A10	44.26	17	17	10

>TRINITY_DN101120_c0_g1_i3 from sample A5 / Example spliced isoform

ATTGTACACATGCCAAGAGCGAGAGCTCATGGATTTGTATTGAACGGTAGTTTTATCTGTGGGGGCACTTTTCCTTTTTCCCTGTCCCTTCTTCTAATCGATCACGCGCTGTGCATGTTTTTTATTTTTGACGGGTCCCATGAACAATAGATTCTGTCAGCTCATACGAACAATAGAAAAAATCGCAGTATTTTTTACAT
ATAAATATAGATAGATAGATGTGTAGGATGAATCCATCTTCATAGATGATCTGCTGCAGCATAAACCTGGAGCTTGAGTGCAGGTACAAATAATTTAGTCCATTAGGCTCTTGGCAGGACCAGTGGTGTGCCAGACTCTAGTATTCTAGTGTGCAACAAGGATGCTGATGAGGGTTGCCAATATGGAGCAGGTCCCGTGT
TCGATCGCCGATGTCAGATCGTCTATGTTGCTCAATGGACACCAGATGCTCAGACGAAGCACAGCCCGCGAGTTTTGCTTCACGAGAAATTTTCCAGGCAGGCAATCGGGCTGCTGCTGGAACGATGGTAATTGCAAAAGGAGAATCGGTCCAATTCGTGCAGTTATCAGCGACAAACCCGCCACGACCACCGGCGTGAC
GCCTCCTGTTGACAAGGGTGCGATGATCAATGTGAAGGCCGTGGCCATCATCAGGAAGAAACTCAAGGAGGACATGAAGGAGAAGATCGAGAATCAGTGGGAGTGTCTGTGTGATGTCATTGGCCAGAACGTCCTTCTGGAGCTAGTCAGTGTTGACATCGATCCAGAGACAAGTTGTGGCAAGAGAACCAAAGAATCTG
CTTTGAAGGGCTGGCTGCTGCAGACTGCTCGTGAAGACAACACAATGGAATGCACTGCAAATTTTACAGTAAGCGCTGACTTTGGTTGTCCGGGTGCTATTCTAGTTACCAATCAACACGACAAAGAAATCTTTTTGGAATCCATTGCTGTGGAAGGGTTCAACAGCGAGCCGATCTACTTCCCTTGCCACTCTTGGGTC
CATTCAAAAAAGGATAATCCCGAGAAAAGAATCTTCTTCAGCAATCAGCCATATTTGCCTTCGAAAACCCCAGATGGGCTAGAGGATATTCGGCAGAAGGACATGAAAGACAAGCGGGGG

>TRINITY_DN101120_c0_g1_i1 from sample A5 / Example unspliced isoform:

ATTGTACACATGCCAAGAGCGAGAGCTCATGGATTTGTATTGAACGGTAGTTTTATCTGTGGGGGCACTTTTCCTTTTTCCCTGTCCCTTCTTCTAATCGATCACGCGCTGTGCATGTTTTTTATTTTTGACGGGTCCCATGAACAATAGATTCTGTCAGCTCATACGAACAATAGAAAAAATCGCAGTATTTTTTACAT
ATAAATATAGATAGATAGATGTGTAGGATGAATCCATCTTCATAGATGATCTGCTGCAGCATAAACCTGgtattttaatccattttgcagtagGAGCTTGAGTGCAGGTACAAATAATTTAGTCCATTAGGCTCTTGGCAGGACCAGTGGTGTGCCAGACTCTAGTATTCTAGTGTGCAACAAGGATGCTGATGAGGGTT
GCCAATATGGAGCAGGTCCCGTGTTCGATCGCCGATGTCAGATCGTCTATGTTGCTCAATGGACACCAGATGCTCAGACGAAGCACAGCCCGCGAGTTTTGCTTCACGAGAAATTTTCCAGGCAGGCAATCGGGCTGCTGCTGGAACGATGGTAATTGCAAAAGGAGAATCGGTCCAATTCGTGCAGTTATCAGCGACAA
ACCCGCCACGACCACCGGCGTGACGCCTCCTGTTGACAAGGGTGCGATGATCAATGTGAAGGCCGTGGCCATCATCAGGAAGAAACTCAAGGAGGACATGAAGGAGAAGATCGAGAATCAGTGGGAGTGTCTGTGTGATGTCATTGGCCAGAACGTCCTTCTGGAGCTAGTCAGTGTTGACATCGATCCAGAGACAAGTT
GTGGCAAGAGAACCAAAGAATCTGCTTTGAAGGGCTGGCTGCTGCAGACTGCTCGTGAAGACAACACAATGGAATGCACTGCAAATTTTACAGTAAGCGCTGACTTTGGTTGTCCGGGTGCTATTCTAGTTACCAATCAACACGACAAAGAAATCTTTTTGGAATCCATTGCTGTGGAAGGGTTCAACAGCGAGCCGATC
TACTTCCCTTGCCACTCTTGGGTCCATTCAAAAAAGGATAATCCCGAGAAAAGAATCTTCTTCAGCAATCAGCCATATTTGCCTTCGAAAACCCCAGATGGGCTAGAGGATATTCGGCAGAAGGACATGAAAGACAAGCGGGGG