sp|Q9M612|NACA_PINTA ----> Nascent polypeptide-associated complex subunit alpha-like protein

Intron 1 GTAAGTATAC ... TGTGTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	30	21	8	10
A2	33.33	27	16	11
A3	28.77	26	7	14
A4	29.17	17	6	8
A5	27.78	13	6	4
A6	29.49	110	54	38
A7	26.85	109	49	31
A8	24.1	126	30	50
A9	24.36	132	51	34
A10	25.08	115	42	35

>TRINITY_DN123809_c2_g3_i2 from sample A2 / Example spliced isoform

TGCCAAAGCAAAAACACAAAAAAATGAGATCAAGGAGCAGTAGTTGTTGTCTGCATGAATCGATTTTCTGTTTGGTACTCGAAACTTACGAGAGATCAGAGTGTAATGCAGAAGGGCGAAGTAAGAATGTCAGAGAGTGGAAAGGGCGGAAACGAGGAAGCTTCAGAGCCGGAACAAGACCAGGACGGCCTCTCTGCCCA
CTCCCCATTTCAAGCTCCTCGTTCCTCTGCATCTTCACTTCCCAAGGAGCTGCCCCAAGTTGAGCTGGAACTGAGGCTGCTGGAAGCTCTGGAGATCTATCACCCCGTCAAGCTACAAGGGATGCACCGACACTTCGTTCTCTTTGGCTTGATGGAATACCTTAAGAAAAGGTTAAATCGGCATTTCTCTCCAGATGAGG
TATTGCAACTATTGGATCGATTCTATAATCTTGAAATGCTGAAACCAGATGATGAGGAGATGGAGGCTTTCAGTCAAGAAGAGGAATTTAGTTTACCTCATTCGATTTTCATGATGAGAGATGATTGAGATGCTGCCATTTTTGTGGTGTTTCTTGGAGCTTTTGTCCTGAAGAAGAGACATACCACAGGATGCTACTAC
TGGGCAGCAGCATATTCTCTTTAATTCATTTCATGTTGATATACCTACCGTATGACCCGATTTTAGATGGACAGATTTATTTACTGTTATCATAGATGAACACATTGTAACTATTATGGTCTGTGGGGTGTTTTCTGTAGACTCTATTGCATACGGAGTACAGAAAAATATGTTATTTCCTAAGTTGCCAGGAAACTTGG
GTACGGAGATGGGGACAGGTACGGGTACGGGGACGGGGACTCCACTTTTTGAAACAGGGTACTTGGGTACGTCGGGGGTACTGTGTGTGTGTGTATCTATATCTATATATCTATATATTATAAAAATAAAACATGTCACGGTCACATTATACATGCAT

>TRINITY_DN123809_c2_g3_i1 from sample A2 / Example unspliced isoform:

TGCCAAAGCAAAAACACAAAAAAATGAGATCAAGGAGCAGTAGTTGTTGTCTGCATGAATCGATTTTCTGTTTGGTACTCGAAACTTACGAGAGATCAGAGTGTAATGCAGAAGGGCGAAGTAAGAATGTCAGAGAGTGGAAAGGGCGGAAACGAGGAAGCTTCAGAGCCGGAACAAGACCAGGACGGCCTCTCTGCCCA
CTCCCCATTTCAAGCTCCTCGTTCCTCTGCATCTTCACTTCCCAAGGAGCTGCCCCAAGTTGAGCTGGAACTGAGGCTGCTGGAAGCTCTGGAGATCTATCACCCCGTCAAGCTACAAGGGATGCACCGACACTTCGTTCTCTTTGGCTTGATGGAATACCTTAAGAAAAGGTTAAATCGGCATTTCTCTCCAGATGAGG
TATTGCAACTATTGGATCGATTCTATAATCTTGAAATGCTGAAACCAGATGATGAGGAGATGGAGGCTTTCAGTCAAGAAGAGGAATTTAGTTTACCTCATTCGATTTTCATGATGAGAGATGATTGAGATGCTGCCATTTTTGTGGTGTTTCTTGGAGCTTTTGTCCTGAAGAAGAGACATgtaagtataccagtataa
aattactaggcgagagatggaaactggatatagtatatccagttaaatcttttttcttggacccagcatccaatttcctcaccttgcagcagaattatgtgcaaaagcttttgggcatgtataatatattggatgtctaagagtacatgtgttgtgtttgtgtgcaaatacatgtataattttactttcattattgtgct
tatgtgggcaatttttttccatggaaaactttgtgggggtggggaaacccaggggatgcttccccacattcatggatggcatccctagtaagttagtaacctgttggcatagactagtgtgcccacctgggggattcgaattgacacctgctggcatgaaactagccaaatgcccaacaacattgtcaaaaaccagtgga
gctaacctcttgggttcatatgtgggcatttatgcacaaacacgtgctagcagattttttctttcccgtgtgcttagttgttaatctttatttcccttttgctggactacagtttctttatcccatctttgcagaagtgaattgcttgtttcttggcagggagtttgatatctaactggtttacaattgtgttgcagACC
ACAGGATGCTACTACTGGGCAGCAGCATATTCTCTTTAATTCATTTCATGTTGATATACCTACCGTATGACCCGATTTTAGATGGACAGATTTATTTACTGTTATCATAGATGAACACATTGTAACTATTATGGTCTGTGGGGTGTTTTCTGTAGACTCTATTGCATACGGAGTACAGAAAAATATGTTATTTCCTAAGT
TGCCAGGAAACTTGGGTACGGAGATGGGGACAGGTACGGGTACGGGGACGGGGACTCCACTTTTTGAAACAGGGTACTTGGGTACGTCGGGGGTACTGTGTGTGTGTGTATCTATATCTATATATCTATATATTATAAAAATAAAACATGTCACGGTCACATTATACATGCAT

Intron 2 GTTAAATCGG ... TTCTCTCCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	52.94	8	7	11
A2	46.67	8	8	6
A3	46.79	29	29	22
A4	55.56	8	10	10
A5	43.86	16	9	16
A6	47.23	62	54	57
A7	47.37	80	68	76
A8	48.63	75	68	74
A9	48.41	65	65	57
A10	48.37	79	72	76

>TRINITY_DN42726_c3_g2_i7 from sample A6 / Example spliced isoform

ATTTTGTTTTCGTGGACTCTTTTTACAACTGCATCTAAGTTCTACCATAGTTACAAATACATCTAGTTTTACTATCTTTATCGGTGTGTAATTAAAATTTAATGTTTTGTGACAAAAAATTGGCCAATTTGGCGATCAAATCCAATCCTTGTAGGTTCCCCATCTGAAAATAAATACCTCTTGCAAGTTGCGATGGATCA
TCTGACCACGTGCCAAAGCAAAAACACAAAAAAATGAGATCAAGGAGCAGTAGTTGTTGTCTGCATGAATCGATTTTCTGTTTGGTACTCGAAACTTACGAGAGATCAGAGTGTAATGCAGAAGGGCGAAGTAAGAATGTCAGAGAGTGGAAAGGGCGGAAACGAGGAAGCTTCAGAGCCGGAACAAGACCAGGACGGCC
TCTCTGCCCACTCCCCATTTCAAGCTCCTCGTTCCTCTGCATCTTCACTTCCCAAGGAGCTGCCCCAAGTTGAGCTGGAACTGAGGCTGCTGGAAGCTCTGGAGATCTATCACCCCGTCAAGCTACAAGGGATGCACCGACACTTCGTTCTCTTTGGCTTGATGGAATACCTTAAGAAAAGATGAGGTATTGCAACTATT
GGATCGATTCTATAATCTTGAAATGCTGAAACCAGATGATGAGGAGATGGAGGCTTTCAGTCAAGAAGAGGAATTTAGTTTACCTCATTCGATTTTCATGATGAGAGATGATTGAGATGCTGCCATTTTTGTGGTGTTTCTTGGAGCTTTTGTCCTGAAGAAGAGACATGTAAGTATACCAGTATAAAATTACTAGGCGA
GAGATGGAAACTGGATATAGTATATCCAGTTAAATCTTTTTTCTTGGACCCAGCATCCAATTTCCTCACCTTGCAGCAGAATTGTGTGCAAAAGCTTTTGGGCATGTATAATATATTGGATGTCTAAGAGTAC

>TRINITY_DN42726_c3_g2_i12 from sample A6 / Example unspliced isoform:

ATTTTGTTTTCGTGGACTCTTTTTACAACTGCATCTAAGTTCTACCATAGTTACAAATACATCTAGTTTTACTATCTTTATCGGTGTGTAATTAAAATTTAATGTTTTGTGACAAAAAATTGGCCAATTTGGCGATCAAATCCAATCCTTGTAGGTTCCCCATCTGAAAATAAATACCTCTTGCAAGTTGCGATGGATCA
TCTGACCACGTGCCAAAGCAAAAACACAAAAAAATGAGATCAAGGAGCAGTAGTTGTTGTCTGCATGAATCGATTTTCTGTTTGGTACTCGAAACTTACGAGAGATCAGAGTGTAATGCAGAAGGGCGAAGTAAGAATGTCAGAGAGTGGAAAGGGCGGAAACGAGGAAGCTTCAGAGCCGGAACAAGACCAGGACGGCC
TCTCTGCCCACTCCCCATTTCAAGCTCCTCGTTCCTCTGCATCTTCACTTCCCAAGGAGCTGCCCCAAGTTGAGCTGGAACTGAGGCTGCTGGAAGCTCTGGAGATCTATCACCCCGTCAAGCTACAAGGGATGCACCGACACTTCGTTCTCTTTGGCTTGATGGAATACCTTAAGAAAAGgttaaatcggcatttctct
ccagATGAGGTATTGCAACTATTGGATCGATTCTATAATCTTGAAATGCTGAAACCAGATGATGAGGAGATGGAGGCTTTCAGTCAAGAAGAGGAATTTAGTTTACCTCATTCGATTTTCATGATGAGAGATGATTGAGATGCTGCCATTTTTGTGGTGTTTCTTGGAGCTTTTGTCCTGAAGAAGAGACATACCACAGG
ATGCTACTACTGGGCAGCAGCATATTCTCTTTAATTCATTTCATGTTGATATACCTACCGTATGACCCGATTTTAGATGGACAGATTTATTTACTGTTATCATAGATGAACACATTGTAACTATTATGGTCTGTGGGGTGTTTTCTGTAGACTCTATTGCATACGGAGTACAGAAAAATATGTTATTTCCTAAGTTGCCA
GGAAACTTGGGTACGGAGATGGGGACAGGTACGGGTACGGGGACGGGGACTCCACTTTTTGAAACAGGGTACTTGGGTACGTCGGGGGTACTATATATATATATATATATATATATAAAACATGTCACATTATGCATGCATATTTCAGATTTGACTAGAAGTTACATAAAATTTGATAAATAACTGTACCAGGCAGTTGT
AACAAATAGTCAAACTGTAATCTGAAGGTACACTTGAAATCTGATTTTAAACACCTATTTGTAATGCTGTTATAGGTGATAAAAAGTTGCAAATGTAATACTGGGTTGATGTGAG