sp|Q5W794|DI192_ORYSJ ----> Protein DEHYDRATION-INDUCED 19 homolog 2

Intron 1 GTACTTCAAT ... CTGTAAGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	29.41	6	5	0
A2	34.43	20	10	11
A3	30.43	16	10	4
A4	44.19	12	18	1
A5	42.86	8	10	2
A6	29.53	68	35	22
A7	37.62	63	49	27
A8	39.32	47.3	41.3	20
A9	32.38	71	49	19
A10	33.77	51	39	13

>TRINITY_DN42640_c0_g2_i4 from sample A6 / Example spliced isoform

TATTGATTATAAATTTTCTAATTAGTAAGTTCTATTGAATATTTTTAAATGTTCTAGTTTCTAAAAAAATTTGATCTATATGTCTATATTTTTAATATATATAATTTAAAATATATATATATATATATACAGGATTCTCTCGATGATCTTCCTGGGATAAACCATTTTGCTCTGTTTTTTCTTTTCCAAACTGAAGGCCG
TGTTTGTTTTTAGCATCGATCCATTTGTTGTCAAACTGCTTTTTAAACCCTAACCCCTGACAAGCATAAACGAATGACGATGCCACACCCATGTGTTCCCCACTCATTCTCATAGGGGGCCACTTTTTCTTTATCCACTTTATTTTATTTTATTTTAAGCCCCTCCGCCATGAACCTTCCACCTCTTTTATGCATGTGAA
AGAGAAAGGAAAAGAATATAAAGTGATAGAGACCAAAGGCAAAGGCAAGGCAAGGCCAGGAATGGATTTAAGAGCCACTCAAGCCGTCCATACATTTGTGCTCTAAAATTCCATTTCTTCGCCAATGACCTTATAACTCCTCTTTGGTCATTCATTTTCCAAGTGCCCTGGAATACGGGGTATTTATTTCAGCAAAGGCC
ATTATTGCTTGCAATTCCTCCGTGATAATAATGGAAGCCGAGTTCTGGACTTCTCGCATGACCGCTGCTAAGCGCCAAGCTGCGTTGAACTCAGTGGAGGATTTTTACTATGATTTGAGGAACCGAGGGAATTTATACAAGGAGTTGAGAAAATAGAGGAATTTTCTAGAAAGTCTGGAAAGTTTTCTGTTTTGATTGTA
GGTTATGCTAAGTCAACTTTTAAGAAAGAATCTCTGCAGTACAGCGGCTTATTTTGAGATAAATGTTCATGAATTTAGGGAAGATGCTCTGTTGAGTACACATGATTATTTTGAGCTAATTTTTCATGAACTAAATGAAGAATGTTTGTTGAGTCAACTGATTATTTTAAACTAAGTTTTCATGAATTTAAGAAAAAAAA
TCTCTGTTCAGCAC

>TRINITY_DN42640_c0_g2_i9 from sample A6 / Example unspliced isoform:

TATTGATTATAAATTTTCTAATTAGTAAGTTCTATTGAATATTTTTAAATGTTCTAGTTTCTAAAAAAATTTGATCTATATGTCTATATTTTTAATATATATAATTTAAAATATATATATATATATATACAGGATTCTCTCGATGATCTTCCTGGGATAAACCATTTTGCTCTGTTTTTTCTTTTCCAAACTGAAGGCCG
TGTTTGTTTTTAGCATCGATCCATTTGTTGTCAAACTGCTTTTTAAACCCTAACCCCTGACAAGCATAAACGAATGACGATGCCACACCCATGTGTTCCCCACTCATTCTCATAGGGGGCCACTTTTTCTTTATCCACTTTATTTTATTTTATTTTAAGCCCCTCCGCCATGAACCTTCCACCTCTTTTATGCATGTGAA
AGAGAAAGGAAAAGAATATAAAGTGATAGAGACCAAAGGCAAAGGCAAGGCAAGGCCAGGAATGGATTTAAGAGCCACTCAAGCCGTCCATACATTTGTGCTCTAAAATTCCATTTCTTCGCCAATGACCTTATAACTCCTCTTTGGTCATTCATTTTCCAAGTGCCCTGGAATACGGGGTATTTATTTCAGCAAAGGCC
ATTATTGCTTGCAATTCCTCCGTGATAATAATGGAAGCCGAGTTCTGGACTTCTCGCATGACCGCTGCTAAGCGCCAAGCTGCGTTGAACTCAGgtacttcaatttgtctccgctaaattgtcgaatttttctttaatttttttcccctgtgcctcgcattttgcaaattttctcaggaattctgaaggttttttggtga
agtggagaaattaaaggaattttctgtaagcagTGGAGGATTTTTACTATGATTTGAGGAACCGAGGGAATTTATACAAGGAGTTGAGAAAATAGAGGAATTTTCTAGAAAGTCTGGAAAGTTTTCTGTTTTGATTGTAGGTTATGCTAAGTCAACTTTTAAGAAAGAATCTCTGCAGTACAGCGGCTTATTTTGAGATA
AATGTTCATGAATTTAGGGAAGATGCTCTGTTGAGTACACATGATTATTTTGAGCTAATTTTTCATGAACTAAATGAAGAATGTTTGTTGAGTCAACTGATTATTTTAAACTAAGTTTTCATGAATTTAAGAAAAAAAATCTCTGTTCAGCAC

Intron 2 GTACTTCAAT ... GAAATTAAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	30.39	5.8	5	0
A2	32.83	21.5	10	11
A3	31.91	16	10	5
A4	43.25	12.5	18	1
A5	42.86	8	10	2
A6	28.07	61.5	35	13
A7	36.41	63.8	49	24
A8	38.9	50.3	41	23
A9	31.14	73	49	17
A10	33.12	49.5	39	10

>TRINITY_DN42640_c0_g2_i8 from sample A6 / Example spliced isoform

TATTGATTATAAATTTTCTAATTAGTAAGTTCTATTGAATATTTTTAAATGTTCTAGTTTCTAAAAAAATTTGATCTATATGTCTATATTTTTAATATATATAATTTAAAATATATATATATATATATACAGGATTCTCTCGATGATCTTCCTGGGATAAACCATTTTGCTCTGTTTTTTCTTTTCCAAACTGAAGGCCG
TGTTTGTTTTTAGCATCGATCCATTTGTTGTCAAACTGCTTTTTAAACCCTAACCCCTGACAAGCATAAACGAATGACGATGCCACACCCATGTGTTCCCCACTCATTCTCATAGGGGGCCACTTTTTCTTTATCCACTTTATTTTATTTTATTTTAAGCCCCTCCGCCATGAACCTTCCACCTCTTTTATGCATGTGAA
AGAGAAAGGAAAAGAATATAAAGTGATAGAGACCAAAGGCAAAGGCAAGGCAAGGCCAGGAATGGATTTAAGAGCCACTCAAGCCGTCCATACATTTGTGCTCTAAAATTCCATTTCTTCGCCAATGACCTTATAACTCCTCTTTGGTCATTCATTTTCCAAGTGCCCTGGAATACGGGGTATTTATTTCAGCAAAGGCC
ATTATTGCTTGCAATTCCTCCGTGATAATAATGGAAGCCGAGTTCTGGACTTCTCGCATGACCGCTGCTAAGCGCCAAGCTGCGTTGAACTCAGGAATTTTCTGTAAGCAGTGGAGGATTTTTACTATGATTTGAGGAACCGAGGGAATTTATACAAGGAGTTGAGAAAATAGAGGAATTTTCTAGAAAGTCTGGAAAGT
TTTCTGTTTTGATTGTAGGTTATGCTAAGTCAACTTTTAAGAAAGAATCTCTGCAGTACAGCGGCTTATTTTGAGATAAATGTTCATGAATTTAGGGAAGATGCTCTGTTGAGTACACATGATTATTTTGAGCTAATTTTTCATGAACTAAATGAAGAATGTTTGTTGAGTCAACTGATTATTTTAAACTAAGTTTTCAT
GAATTTAAGAAAAAAAATCTCTGTTCAGCAC

>TRINITY_DN42640_c0_g2_i9 from sample A6 / Example unspliced isoform:

TATTGATTATAAATTTTCTAATTAGTAAGTTCTATTGAATATTTTTAAATGTTCTAGTTTCTAAAAAAATTTGATCTATATGTCTATATTTTTAATATATATAATTTAAAATATATATATATATATATACAGGATTCTCTCGATGATCTTCCTGGGATAAACCATTTTGCTCTGTTTTTTCTTTTCCAAACTGAAGGCCG
TGTTTGTTTTTAGCATCGATCCATTTGTTGTCAAACTGCTTTTTAAACCCTAACCCCTGACAAGCATAAACGAATGACGATGCCACACCCATGTGTTCCCCACTCATTCTCATAGGGGGCCACTTTTTCTTTATCCACTTTATTTTATTTTATTTTAAGCCCCTCCGCCATGAACCTTCCACCTCTTTTATGCATGTGAA
AGAGAAAGGAAAAGAATATAAAGTGATAGAGACCAAAGGCAAAGGCAAGGCAAGGCCAGGAATGGATTTAAGAGCCACTCAAGCCGTCCATACATTTGTGCTCTAAAATTCCATTTCTTCGCCAATGACCTTATAACTCCTCTTTGGTCATTCATTTTCCAAGTGCCCTGGAATACGGGGTATTTATTTCAGCAAAGGCC
ATTATTGCTTGCAATTCCTCCGTGATAATAATGGAAGCCGAGTTCTGGACTTCTCGCATGACCGCTGCTAAGCGCCAAGCTGCGTTGAACTCAGgtacttcaatttgtctccgctaaattgtcgaatttttctttaatttttttcccctgtgcctcgcattttgcaaattttctcaggaattctgaaggttttttggtga
agtggagaaattaaagGAATTTTCTGTAAGCAGTGGAGGATTTTTACTATGATTTGAGGAACCGAGGGAATTTATACAAGGAGTTGAGAAAATAGAGGAATTTTCTAGAAAGTCTGGAAAGTTTTCTGTTTTGATTGTAGGTTATGCTAAGTCAACTTTTAAGAAAGAATCTCTGCAGTACAGCGGCTTATTTTGAGATA
AATGTTCATGAATTTAGGGAAGATGCTCTGTTGAGTACACATGATTATTTTGAGCTAATTTTTCATGAACTAAATGAAGAATGTTTGTTGAGTCAACTGATTATTTTAAACTAAGTTTTCATGAATTTAAGAAAAAAAATCTCTGTTCAGCAC

Intron 3 GTAATAAACA ... CCTTCTTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	43.02	6.8	4.5	5
A2	18.01	11.3	1	2.5
A3	21.17	12.3	5.5	3.5
A4	32.74	7.8	3.5	5.5
A5	6.67	11.8	1	1
A6	14.56	22.3	7	4.5
A7	24.63	32.5	2	16
A8	16.4	43	2.5	10.5
A9	13.09	43.5	5.5	7.5
A10	19.09	34.5	0.5	9.5

>TRINITY_DN102992_c0_g2_i3 from sample A4 / Example spliced isoform

GTTTGATTCATAAAAAAAGAGATCAATGTACAGCTATATTTCTAAATCCGTGATTTTGAGCAAAATTGCCTTATAAAGCCAAAATTCAAGCCCAAAACTCTTATAATAGCAGCGTCGAAGCAAACTCTTCAGCAGCAGCAGGCCAATCGGGTCATTATCCACAACCAGGACACTTATACCAATAGGAAAATCATCATTAG
CCTACTCATTGCATCAGTTTCCATAATCAGGAAGCATAATATGCTTGAGTCCATGATTTCAAAATTTTCCACATCCATGATGGGTAGGCCCAACCTTTTTAATGAAAATGGCTTATTTCAATCCTCTATTACATCAATATTGAAAGAAGAATTGAATTCCCCTGAGATACATACCAGAACTAAGCGTTGATGTACCCTGA
ATCCATCATCTAGAACCAGAGTTTCATAAAGGAAGCAATACTTAAATCATATGGAGATTCATTGGGAAGAGCTAATGCAGACAAAGCTTCTTCTGTTCTATCAGCAGAGTGTTTCTTTGCATAGTCAATAGAACCAGGTAGAACCCTCTGAATAGTAGTACTCATTAGCTGAAAGTGATTACTAAGATAAGTAAGCAACA
TGCCAATGTTAAAGTTATTCAAATAAGATCTTAGCCTTAGGTGCCCTACATTAAAATAAATCAAAGACTTACATGTCAGTTGCTGCCTCAAAGTTGCTTGCCCGAAGACTACTTTCAAAGTCTTCAAAGCTTTGTGGATCACAGTGGCTGGGGAGCCTATCCATGATGGCAATTTCCAATTTATTCATTGCATCTTCTAT
AAATTCTGGAACACACTTCAGGGAAGCTGCATGACCTGCATTAACTGCAACTGTGTCTACCATCAGATCCTTTGATGGTGTCTGATGAGGTAAATACCTAAATCAAAGCTAAAGGTTTTTTGCTTGTGCAAGATGCCAAAAATGTATTTATCAGTGCATATTCAAACCTTACTGCAGCATTGTCAGCACTAAGGTAACTG
TCCTTTTACTCTATACATATTTGATTTTCTGTATAATATAAGTCACTAAGTGTACTTTCTCCCAAAAAACCTGTTGGCCAGATGCATCTTTTCTTTATTCATGAACATTACTTCTTGAAAGCTTTGAAACTGTACTTCATTTGGTTTCACTTTCTGACCATTCAGTCCAGTGCAAACTGTTTAGATTGATTCCTTATACT
ATGTCTAACTCTAATAAAGAAGAAAGACCTTTCCTGATGATTGCAGTGCTTTATCTAGCTTCCTTAGATGGAACCGCTTTTCATATTAAAGTAATGGTAATCTCATCCTCAAGCCTATGATCATCCCTTATTTTACTAGATGTGGTTGAGCTCAAACACAGGGTTTTGTTTGGGTGCTTAACACTTGCAAATATTTGAAG
ACGAGGAGGATCTCCCCTACATGCATTCTGTCTTTCATGTCTGAGTAAGCCTTGTCTATATAAGAATAGCAATGCAGGAAGAAGACAAATATTGAAATAGTAGTAATGCACTCAACATTCTGGTATATACAATTACAGTTTGTGCAATGCTACAATCTGCTCTAGTAGAGTCACTTGCCCATTTTGACCACTGTTGTCAT
TTTTAACGAGGCAATATATCTTTATATTTTCTAAATCTAATTGATTTGTTTTATGGTGCAGGCATGCCCTATCTGTGGGGTTAATATTCGGAATGATATTGTGGGGCATGTTACATCTCAGCATTCATTCGTTTAAGATATCCTGTACTTCTTTTAATTATACAAAATTAGTAAAATATGTATTTTACTGTGTTGGATTG
TGTGGTTTGGATCTAGATAAATTCAGATTTATTTTTGGGTGTAATATTTAGTTTGGACTGTTGATAGATTTTCTTTTTGGTTACCTCTGTCTCCATTTGCTATTAAGATGCCAGGTTATGGGCAAAATGTTTGAGAGTAGTTTGAAAATATGTTTTTCCTTTAATTTGTTCCTTAACCACAAGTCATGGGCAGCGCAGAA
GGAAATATCTTAGAGGTAGGATACAACCTAATTCAGCTCCAGATAATATGGTAGGTTATGGGCAAAATGTTTGAGAGTAGTTTGAGAATATGTTTTTCCATTAATTTGTTCCTTAACCACAAGGCATGGATAGCGCAGAAGGAAATATTTTAGAGGTAGGATACAACCTAATTCAGCTCCAGGTAGGGAGTGGCTGCATT
CTTCTGTTGGAGGAGGTTCCTCGCGTTTGGGTGGATGTTCCTCTAATGATGCACCAGATCCTCTACTTTCATCTCTCAGTTATGGGTTGCCAATTATTGAATCCCATGAACAAGAGAAAACAGCTTCCTCTACTGATGATATTTCAGCAAAGAACACTTCAGATTCAGAGGATGTAATCAGATTGAAGAGCAAATATCAG
CTGTAAAAGCTTGGGAACTGGCTCGGTGATTTCCTGATCTAAAGTAGCAGATACTCATTTTTATTTTTACGTGATGGGAATTAATAGCTTAAAGCAACTTGTTTAGCGGGCATGTAATTTCTACCTTTGGAAGAGCTGAGCCGAACTCTTTTCCTTGTTTTCTCTCAAGGATTTTGGTGCCAATTCTTCTGTCAGTGAAG
AGGAATCAAAGCGTATGTTTGAAGAAGGGCTGCAACGAGCTCAATTTGTGCAACAACTTGTTTTGTCTACTATATTACCTGATGAATTTTAGGCTTTAGTTGGGTAGACACTGGGAATTTTCCTCCTGATTTTCATGATCACAGCACTAACAAGCAGTGCAGAAAAGTCCATTGCACATCAATGGGCTGTTCTTGGATTG
GAGTGTAAGAGCCTGACCAGAATCATGTGTTGGTTCTGGTATAATAGGTGAAAAAGGCTTAGGTTGCTTCTGCTGACCTAAAGTCCTTACATTTCAGAGGAATTACAAAGTTTACTGCCATATGGTGGTTATGGGCGTTAGATTCTACCAGATGTAGCTACTGGGTAGATTTAACGATCTCCAAGCTTTGCACAATGTAA
AGCATGTTAAAAAAAATCAGATGCAACTCTCAAG

>TRINITY_DN102992_c0_g2_i12 from sample A4 / Example unspliced isoform:

GTCGTCCTGCCCCCTAGTATGGTTGTCTGAGTATAGCATTATAGTTTGGCCTTGTATTTGTGATGCTCTAGGGATGAAGTGCATCTGCAAAATTTTTCAACTTCTTGTATAGTTTTTCTTTATTATTTCATATTTAACACATGTCCTACAGATTGAAGAGCAAATATCAGCTGTAAAAGCTTGGGAACTGGCTCGGTGAT
TTCCTGATCTAAAGTAGCAGATACTCATTTTTATTTTTACGTGATGGgtaataaacatgaatagcatcatttgattgaagtttagaatagtctatttcttatgtttaagactttaagtcgcttgtacagtatccttgaaacttaggcagtgtatatttttgtctgtttccttgttgccttcttcagGAATTAATAGCTTA
AAGCAACTTGTTTAGCGGGCATGTAATTTCTACCTTTGGAAGAGCTGAGCCGAACTCTTTTCCTTGTTTTCTCTCAAGGATTTTGGTGCCAATTCTTCTGTCAGTGAAGAGGAATCAAAGCGTATGTTTGAAGAAGGGCTGCAACGAGCTCAATTTGTGCAACAACTTGTTTTGTCTACTATATTACCTGATGAATTTTA
GGCTTTAGT

Intron 4 GTGATTTCCT ... CCTTCTTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	86.08	1	7.8	5
A2	17.05	15.8	4.5	2.3
A3	62.01	2.3	2.5	2.8
A4	21.3	10.8	3.8	3.3
A5	8.85	5.5	0.8	0.5
A6	27.68	13.3	7	2.8
A7	11.21	43	3.3	9.5
A8	10.32	41.8	4.3	6.3
A9	12.97	28.3	4.3	4.8
A10	11.7	31.8	3	6.3

>TRINITY_DN45488_c0_g1_i4 from sample A7 / Example spliced isoform

ATCAATACTTTTGCCTGGACGATTTGGAGGGAGATGATACACGCATAGACTTCCTTTGCCCATTTTGCTACGTGGATTTTGATATAGCATCCCTCTGTTGCCATCTTGAAGAGGAACACTGCCTTGATACTACAGTTGCGGCATGCCCTGTCTGTGCGGCTAATGTTGGGAATGATATTGTGGGGCATATTACATCTCAG
CACAGTCATTTGTTTAAGGGACAGCGCAGAAGGAAATATCTTAGAGGTAGGATACAATCTAATTCGGCTCCAGGTAGGGAGCGGCTGCATTCTTCTGTAGTTGGAGGTTCCTCGCGCTTGGGAGGCTTCTCCTCTAATGATGCACCAGATCCTCTACTTTCATCTCTCAGTTATGGGTTGCCAATTATTGAATCCCATGA
ACAAGAGAAAACAGCTTCCTCTACTGATGATATTTCAGCAAAGAACACTTCAGATTCAGAGGATGTAATCAGATTGAAGAGCAAATATCAGCTGTAAAAGCTTGGGAACTGGCTCGGAATTAATAGCTTAAAGCAACTTGTTTAGCGGGCATGTAATTTCTACCTTTGGAAGAGCTGAGCCGAACTCTTTTCCTTGTTTT
CTCTCAAGGATTTTGGTGCCAATTCTTCTGTCAGTGAAGAGGAATCAAAGCGTATGTTTGAAGAAGGGCTGCAACGAGCTCAATTTGTGCAACAACTTGTTTTGTCTACTATATTACCTGATGAATTTTAGGCTTTAGTGGGGGAGACACTGGGAATTTTCCTCCTGATTTTCATGATCACAGCACTAACAAGCAGTGCA
GAAAAGTCCATTGCACATCAATGGGCTGTTCTTGGATTGGAGTGTAAGAGCCTGACCAGAATCATGTGTTGGTTCTGGTATAATAGGTGAAAAAGGCTTAGGTTGCTTCTGCTGACCTAAAGTCCTTACATTTCAGAGGAATTACAAAGTTTACTGCCATATGGTGGTTATGGGCGTTAGATTCTACCAGATGTAGCTAC
TGGGTAGATTTAACGATCTCCAAGCTTTGCACAATGTAAAGCATGTTAAAAAAAATCAGATGCAACTCTCAAGCATATTTGGTCCTTATTTATAGTATTTGTAAAAATGCTGTTCTTGTCAGATTATAGTTTTCATTCAGCAATGATGTTCCAGAGCATTTGTTGTGTGGATGTCAAATTGACTCTTCTTGTCTCATGTC
AATATCTTTTTACTGTGTGCGTATGTCCTAAACTTAATATTGTTGTCTTGCTTCCTCTAGTAGCACAAACATATGGGTATATCTTTTTCTGTTGAAAGATGGGAAAAGCCCACAGTATAATGAAGTTCATCAAAGGTAGGCTTTGCCTGTATACATTCACTACAATAGATCATTTTTATAGAAATTGGATACTCC

>TRINITY_DN45488_c0_g1_i2 from sample A7 / Example unspliced isoform:

CTGTCTCATATTAAAGTCTCAACAAAGTCGTGTGAAATAAATCAAAACCATATATTTATTTGTCTCAATTTAACAGTCGGTACAAAGTGGTCCTGCCCCCTAGTTTGGTTGTCTGAGTATAGCATTATAGTTTGGCCTTGTATTTGTGATGCTGTAGGGATGAAGTGCATCTGCAAAATTTTTCAACTTCTTGTATAGTT
TTTCTTTATTATTTCATATTTAACACATGTCCTACAGATTGAAGAGCAAATATCAGCTGTAAAAGCTTGGGAACTGGCTCGgtgatttcctgatctaaagtagcagatactcatttttatttttacgtaatgggtaataaacatgaatagcatcatttgattgaagtttagaatagtctatttcttatgtttaagacttt
aagtcgcttgtacagtatccttgaaacttaggcagtgtgtatttttgtctgtttccttgttgccttcttcagGAATTAATAGCTTAAAGCAACTTGTTTAGCGGGCATGTAATTTCTACCTTTGGAAGAGCTGAGCCGAACTCTTTTCCTTGTTTTCTCTCAAGGATTTTGGGTACCTCTTCTTTCTTCCTCTTTTTCTG
ATTTTTTGATTCTGATGATTACGTGCTATAGCATTGATGTATCAGTTGATATTTTTCTTATTCTCGTGTCTTCTGCCTTCTGAT

Intron 5 GTACTTCAAT ... ATTTTCTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	50	5	5	0
A2	45.45	12	10	2
A3	55.56	8	10	4
A4	58.06	13	18	1
A5	55.56	8	10	2
A6	45.45	42	35	7
A7	53.26	43	49	22
A8	51.25	39	41	19
A9	44.55	61	49	10
A10	50	39	39	12

>TRINITY_DN44235_c0_g6_i1 from sample A8 / Example spliced isoform

CTCCGCCATGAACCTTCCACCTCTTTTATGCATGTGAAAGAGAAAGGAAAAGAATATAAAGTGATAGAGACCAAAGGCAAAGGCAAGGCAAGGCCAGGAATGGATTTAAGAGCCACTCAAGCCGTCCATACATTTGTGCTCTAAAATTCCATTTCTTCGCCAATGACCTTATAACTCCTCTTTGGTCATTCATTTTCCAA
GTGCCCTGGAATACGGGGTATTTATTTCAGCAAAGGCCATTATTGCTTGCAATTCCTCCGTGATAATAATGGAAGCCGAGTTCTGGACTTCTCGCATGACCGCTGCTAAGCGCCAAGCTGCGTTGAACTCAGGAATTCTGAAGGTTTTTTGGTGAAGTGGAGAAATTAAAGGAATTTTCTGTAAGCAGTGGAGGATTTTT
ACTATGATTTGAGGAACCGAGGGAATTTATACAAGGAGTTGAGAAAATAGAGGAATTTTCTAGAAAGTCTGGAAAGTTTTCTGTTTTGATTGTAGGTTATGCTAAGTCAACTTTTAAGAAAGAATCTCTGCAGTACAGCGGCTTATTTTGAGATAAATGTTCATGAATTTAGGGAAGATGCTCTGTTGAGTACACATGAT
TATTTTGAGCTAATTTTTCATGAACTAAATGAAGAATGTTTGTTGAGTCAACTGATTATTTTAAACTAAGTTTTCATGAATTTAAGAAAAAAAATCTCTGTTCAGCG

>TRINITY_DN44235_c0_g6_i3 from sample A8 / Example unspliced isoform:

CTCCGCCATGAACCTTCCACCTCTTTTATGCATGTGAAAGAGAAAGGAAAAGAATATAAAGTGATAGAGACCAAAGGCAAAGGCAAGGCAAGGCCAGGAATGGATTTAAGAGCCACTCAAGCCGTCCATACATTTGTGCTCTAAAATTCCATTTCTTCGCCAATGACCTTATAACTCCTCTTTGGTCATTCATTTTCCAA
GTGCCCTGGAATACGGGGTATTTATTTCAGCAAAGGCCATTATTGCTTGCAATTCCTCCGTGATAATAATGGAAGCCGAGTTCTGGACTTCTCGCATGACCGCTGCTAAGCGCCAAGCTGCGTTGAACTCAGgtacttcaatttgtctccgctaaattgtcgaatttttctttaatttttttcccctgtgcctcgcattt
tgcaaattttctcagGAATTCTGAAGGTTTTTTGGTGAAGTGGAGAAATTAAAGGAATTTTCTGTAAGCAGTGGAGGATTTTTACTATGATTTGAGGAACCGAGGGAATTTATACAAGGAGTTGAGAAAATAGAGGAATTTTCTAGAAAGTCTGGAAAGTTTTCTGTTTTGATTGTAGGTTATGCTAAGTCAACTTTTAA
GAAAGAATCTCTGCAGTACAGCGGCTTATTTTGAGATAAATGTTCATGAATTTAGGGAAGATGCTCTGTTGAGTACACATGATTATTTTGAGCTAATTTTTCATGAACTAAATGAAGAATGTTTGTTGAGTCAACTGATTATTTTAAACTAAGTTTTCATGAATTTAAGAAAAAAAATCTCTGTTCAGCG

Intron 6 GTACTTCAAT ... TTTGGTGAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	75	2.5	5	0
A2	64.39	7	10	2
A3	65.45	8	10	3
A4	76.4	7	18	1
A5	77.78	4	10	2
A6	60.13	30	35	6
A7	63.95	33	49	21
A8	59.64	31.5	41	21
A9	60.45	40.5	49	11
A10	67.41	24	39	10

>TRINITY_DN42640_c0_g2_i2 from sample A6 / Example spliced isoform

TCTCCGTGATAATTATGGAAGCCGAGTTCTGGACTTCTCGCATGACCGCTGCTAAGCGCCAAGCTGCGTTGAACTCAGTGGAGAAATTAAAGGAATTTTCTGTAAGCAGTGGAGGATTTTTACTATGATTTGAGGAACCGAGGGAATTTATACAAGGAGTTGAGAAAATAGAGGAATTTTCTAGAAAGTCTGGAAAGTTT
TCTGTTTTGATTGTAGGTTATGCTAAGTCAACTTTTAAGAAAGAATCTCTGCAGTACAGCGGCTTATTTTGAGATAAATGTTCATGAATTTAGGGAAGATGCTCTGTTGAGTACACATGATTATTTTGAGCTAATTTTTCATGAACTAAATGAAGAATGTTTGTTGAGTCAACTGATTATTTTAAACTAAGTTTTCATGA
ATTTAAGAAAAAAAATCTCTGTTCAGCAC

>TRINITY_DN42640_c0_g2_i9 from sample A6 / Example unspliced isoform:

TATTGATTATAAATTTTCTAATTAGTAAGTTCTATTGAATATTTTTAAATGTTCTAGTTTCTAAAAAAATTTGATCTATATGTCTATATTTTTAATATATATAATTTAAAATATATATATATATATATACAGGATTCTCTCGATGATCTTCCTGGGATAAACCATTTTGCTCTGTTTTTTCTTTTCCAAACTGAAGGCCG
TGTTTGTTTTTAGCATCGATCCATTTGTTGTCAAACTGCTTTTTAAACCCTAACCCCTGACAAGCATAAACGAATGACGATGCCACACCCATGTGTTCCCCACTCATTCTCATAGGGGGCCACTTTTTCTTTATCCACTTTATTTTATTTTATTTTAAGCCCCTCCGCCATGAACCTTCCACCTCTTTTATGCATGTGAA
AGAGAAAGGAAAAGAATATAAAGTGATAGAGACCAAAGGCAAAGGCAAGGCAAGGCCAGGAATGGATTTAAGAGCCACTCAAGCCGTCCATACATTTGTGCTCTAAAATTCCATTTCTTCGCCAATGACCTTATAACTCCTCTTTGGTCATTCATTTTCCAAGTGCCCTGGAATACGGGGTATTTATTTCAGCAAAGGCC
ATTATTGCTTGCAATTCCTCCGTGATAATAATGGAAGCCGAGTTCTGGACTTCTCGCATGACCGCTGCTAAGCGCCAAGCTGCGTTGAACTCAGgtacttcaatttgtctccgctaaattgtcgaatttttctttaatttttttcccctgtgcctcgcattttgcaaattttctcaggaattctgaaggttttttggtga
agTGGAGAAATTAAAGGAATTTTCTGTAAGCAGTGGAGGATTTTTACTATGATTTGAGGAACCGAGGGAATTTATACAAGGAGTTGAGAAAATAGAGGAATTTTCTAGAAAGTCTGGAAAGTTTTCTGTTTTGATTGTAGGTTATGCTAAGTCAACTTTTAAGAAAGAATCTCTGCAGTACAGCGGCTTATTTTGAGATA
AATGTTCATGAATTTAGGGAAGATGCTCTGTTGAGTACACATGATTATTTTGAGCTAATTTTTCATGAACTAAATGAAGAATGTTTGTTGAGTCAACTGATTATTTTAAACTAAGTTTTCATGAATTTAAGAAAAAAAATCTCTGTTCAGCAC

Intron 7 GTAATGTAGT ... TGTGCTCAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	100	0	2	7
A2	100	0	0	8
A3	38.46	4	5	0
A4	47.37	5	7	2
A5	28.57	5	4	0
A6	36.36	7	8	0
A7	45.45	6	6	4
A8	38.1	13	13	3
A9	48.57	9	15	2
A10	41.46	12	16	1

>TRINITY_DN40696_c2_g1_i21 from sample A9 / Example spliced isoform

ATTTTGCTCTGTTTTTTCTTTTCCAAACTGAAGGCCGTGTTTGTTTTTAGCATCGATCCATTTGTTGTCAAACTGCTTTTTAAACCCTAACCCCTGACAAGCATAAACGAGTGACGATGCCACACCCATGTGTTCCCCACTCATTCTCATAGGGGGCCACTTTTTCTTTATCCACTTTATTTTATTTTATTTTATTTTAA
GCCCCTCCGCCATGAACCTTCCACCTCTTTTATGCATGTGAAAGAGAAAGGAAAAGAATATAAAGTGATAGAGACCAAAGGCAAAGGCAAGGCAAGGCCAGGAATGGATTTAAGAGCCACTCAAGCCGTCCATACATTTGTGCTCTAAAATTCCATTTCTTCGCCAATGACCTTATAACTCCTCTTTGGTCATTCATTTT
CCAAGTGCCCTGGAATACGGGGTATTTATTTCAGCAAAGGCCATTATTGCTTGCAATTCCTCCGTGATAATAATGGAAGCCGAGTTCTGGACTTCTCGCATGACCGCTGCTAAGCGCCAAGCTGCGTTGAACTCAGATCAATACTTTTGCCTGGACGATTTGGAGGGAGATGATACACGCATAGACTTCCTTTGCCCATT
TTGCTACGTGGATTTTGATATAGCATCCCTCTGTTGCCATCTTGAAGAGGAACACTGCCTTGATACTACAGTTGCGGCATGCCCTGTCTGTGCGGCTAATGTTGGGAATGATATTGTGGGGCATATTACATCTCAGCACAGTCATTTGTTTAAGGGACAGCGCAGAAGGAAATATCTTAGAGGTAGGATACAATCTAATT
CGGCTCCAGGTAGGGAGCGGCTGCATTCTTCTGTAGTTGGAGGTTCCTCGCGCTTGGGAGGCTTCTCCTCTAATGATGCACCAGATCCTCTACTTTCATCTCTCATTTATGGGTTGCCAATTATTGAATCCCATGAACAAGAGAAAACAGCTTCCTCTACTGATGATATTTCAGCAAAGAACACTTCAGATTCAGAGGAT
GTAATCAGATTGAAGAGCAAATATCAGCTGTAAAAGCTTGGGAACTGGCTCGGTGATTTCCTGATCTAAAGTAGCAGATACTCATTTTTATTTTTACGTGATGGGAATTAATAGCTTAAAGCAACTTGTTTAGCGGGCATGTAATTTCTACCTTTGGAAGAGCTGAGCCGAACTCTTTTCCTTGTTTTCTCTCAAGGATT
TTGGATTGCAAACGTTGTGGGTGGATGCTATTGTCTATGAAAAGAGAAGTCAATCAACAAAATTTCTGCATCGTTTGTTAGTGAAACAAAAGGGCATTCCACATTTCTTAATGGAAGAGGCCATTCTTCATTTCCTCTAGATTGAACTATGACCACATTTTTGAGTAAAGGTGAAGATTATTTCGATGGCAGACAATTTC
TAGATATCATATTTATATTACATCATGATATGCCAGTAATTCTCAAAATAAACACCATCTTGGGAAAATTAGAAGTTGCAATACCAATGGAAGTCTTAATCTTGATATAAATAGCGGGA

>TRINITY_DN40696_c2_g1_i7 from sample A9 / Example unspliced isoform:

TAGGGATGAAGTGCATCTGCAAAATTTTTCAACTTCTTGTATAGTTTTTCTTTATTATTTCATATTTAACACATGTCCTACAGATTGAAGAGCAAATATCAGCTGTAAAAGCTTGGGAACTGGCTCGGTGATTTCCTGATCTAAAGTAGCAGATACTCATTTTTATTTTTACGTAATGGGAATTAATAGCTTAAAGCAAC
TTGTTTAGCGGGCATGTAATTTCTACCTTTGGAAGAGCTGAGCCGAACTCTTTTCCTTGTTTTCTCTCAAGGATTTTGGgtaatgtagtgaatgaacttgctgccagttcaacattaaaatttaggattatgagttatgttgtgtttagaatgagcattttctttgttgaagctgaacttgattttttttagtattaact
tctgatagctttagggttcttgaataccaaatgacatgttaattttgtgctcaagATTGCAAACGTTGTGGGTGGATGCTATTGTCTATGAAAAGAGAAGTCAATCAACAAAATTTCTGCATCGTTTGTTAGTGAAACAAAAGGGCATTCCACATTTCTTAATGGAAGAGGCCATTCTTCATTTCCTCTAGATTGAACTA
TGACCACATTTTTGAGTAAAGGTGAAGATTATTTCGATGGCAGACAATTTCTAGATATCATATTTATATTACATCATGATATGCCAGTAATTCTCAAAATAAACACCATCTTGGGAAAATTAGAAGTTGCAATACCAATGGAAGTCTTAATCTTGATATAAATAGCGGGA