sp|Q8RWE5|PIRL8_ARATH ----> Plant intracellular Ras-group-related LRR protein 8

Intron 1 GTAAGTCTTC ... GTTGAATTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	82.14	5	24	22
A2	74.47	6	22	13
A3	62.16	7	7	16
A4	100	0	13	8
A5	84.62	1	6	5
A6	78.42	15	50	59
A7	72.39	45	121	115
A8	69.3	64	120	169
A9	78.35	38	127	148
A10	75.94	32	103	99

>TRINITY_DN40625_c0_g2_i5 from sample A9 / Example spliced isoform

ACATATGAACAAACTCGGATATCTGCCTGAAGACATTGACAACCTGACGCGAGTTTTAACCAGATTAAAACCCTTCCGGATTTCATTGGAGGGCTGGTGAAATTGAAACGACTAAATCTGGAGGGTAACCCGCTAATTCGACCACCGCCAGATATAGTTAGAGATGGTGTGGAAGCAATTAAGCAATACATGAGCAAAAG
CGCCACCAAGAGACTCGTACCACGTAGCGTTTCAAGTCTGGGAGGCAAACTGGCACGTAAGTGGATATCTTGC

>TRINITY_DN40625_c0_g2_i9 from sample A9 / Example unspliced isoform:

TGTGGATACGCCAAAATTGTTGTTAGAGCACATGTTTGTCACGATATTAATGAAAATAATATCCACACCAGAATCTACTCGATAGAATGCTATTACGTTAACTCATCATTATATTCAACGAAAGTAGGTCGTATGTGGCTTTCTATTTACCAACTAACTTATGATAAACTTATCAACTCCGCCTAATCACAACAGGGCTA
TCCCTGTTGCAATCTTTCCAGACACAGCCAAGGTTCGAAACTCCGCAACAATGGCGTCGGCAAGGGCTGCAGCAGAGGAGGGAAGCCTCGGAACACGGCTGAGTCACGATCTTGTCAAAGGATCGCAGAAGGCCGTATCCCGCATTGAGTCAGAATTCGCATCGAAGATCAAAGCCATCGCAGCCCTCCCCAACCGCCCC
GACATGGATATCTCGCTCTGCAACACTGCCAAGGTTTCAATGGAGGCCGAGGCGAAGAGAATTGCAGAAGAAGAAAAGGCTCCGCACTTATCCGTTATACAGGTCAAGAAGTCCGAGAATCCACCAATCTCAAGAAGTACTGAAGCTGATATTCAGGGAAATATGAATCTAGACCTTCAACAGCTTCTCCACGAACTTAG
AAGGCTTCTACTCTTTAACTTGCCAGACTCTGCACAAAAGCTATCGACCATCGCTAAACTCGACCTTTCTAATAACAACATGAAGTGTCTTACCGAATCCGTGACAAGTCTGGTGAATTTAAGAACTCTCGACGTCCATTCGAACCAGCTCACATCGCTGCCGCATTCCATGGGACGATTGCAGAAGCTTAAAATCCTCA
ATATTTCTGGAAACTTATTTGCCGAATTGCCAGAAAGTGTTCAAAATTGCCGTTCGCTGGAAGAGCTGATTGCTGATTTCAACAAGCTACAGCAGCTTCCTGAAACGCTGGGCATCAAACTGTTTAACCTGCAGAAGCTCTCTGTTCATTCCAACAAACTTTCTTATCTCCCTTCTTCCATCTCATGCCTGATGTCTCTG
CGTGTTTTGGACGTACATATGAACAAACTCGGATATCTGCCTGAAGACATTGACAACCTGgtaagtcttcaaattcttaatgttagttgcaattttggctacttgaacgccttgccggattcgatctgtgggctgtgttcactcgttgaattagACGCGAGTTTTAACCAGATTAAAACCCTTCCGGATTTCATTGGAGG
GCTGGTGAAATTGAAACGACTAAATCTGGAGGGTAACCCGCTAATTCGACCACCGCCAGATATAGTTAGAGATGGTGTGGAAGCAATTAAGCAATACATGAGCAAAAGCGCCACCAAGAGACTCGTACCACGTAGCGTTTCAAGTCTGGGAGGCAAACTGGCACGTAAGTGGATATCTTGCATCGGTGAAGCTGACGAAA
TATGACATCTGGCGAAATGATGACGGGGCAAGAGTATATATGTGAATAACAGTGATATAAGAGTTTCGTTGGGTTGAAAAAACAATATACTCTGTTTGTTGGAACGTGG

Intron 2 GTAAGTGCAG ... CTTGCTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	29.82	20	13	4
A2	34.69	16	9	8
A3	25.93	30	8	13
A4	20	26	9	4
A5	33.33	11	6	5
A6	22.54	67	12	27
A7	22.01	163	47	45
A8	21.39	136	30	44
A9	21.67	141	32	46
A10	20.66	144	40	35

>TRINITY_DN96916_c0_g1_i1 from sample A2 / Example spliced isoform

TCACAACAGGGCTATCCCTGTTGCAATCTTTCCAGACACAGCCAAGGTTCGAAACTCCGCAACAATGGCGTCGGCAAGGGCTGCAGCAGAGGAGGGAAGCCTCGGAACACGGCTGAGTCACGATCTTGTCAAAGGATCGCAGAAGGCCGTATCCCGCATTGAGTCAGAATTCGCATCGAAGATCAAAGCCATCGCAGCCC
TCCCCAACCGCCCCGACATGGATATCTCGCTCTGCAACACTGCCAAGGTTTCAATGGAGGCCGAGGCGAAGAGAATTGCAGAAGAAGAAAAGGCTCCGCACTTATCCGTTATACAGGTCAAGAAGTCCGAGAATCCACCAATCTCAAGAAGTACTGAAGCTGATATTCAGGGAAATATGAATCTAGACCTTCAACAGCTT
CTCCACGAACTTAGAAGGCTTCTACTCTTTAACTTGCCAGACTCTGCACAAAAGCTATCGACCATCGCTAAACTCGACCTTTCTAATAACAACATGAAGTGTCTTACCGAATCCGTGACAAGTCTGGTGAATTTAAGAACTCTCGACGTCCATTCGAACCAGCTCACATCGCTGCCGCATTCCATGGGACGATTGCAGAA
GCTTAAAATCCTCAATATTTCTGGAAACTTATTTGCCGAATTGCCAGAAAGTGTTCAAAATTGCCGTTCGCTGGAAGAGCTGATTGCTGATTTCAACAAGCTACAGCAGCTTCCTGAAACGCTGGGCATCAAACTGTTTAACCTGCAGAAGCTCTCTGTTCATTCCAACAAACTTTCTTATCTCCCTTCTTCCATCTCAT
GCCTGATGTCTCTGCGTGTTTTGGACGTACATATGAACAAACTCGGATATCTGCCTGAAGACATTGACAACCTGGTAAGTCTTCAAATTCTTAATGTTAGTTGCAATTTTGGCTACTTGAACGCCTTGCCGGATTCGATCTGTGGGCTGTGTTCACTCGTTGAATTAGACGCGAGTTTTAACCAGATTAAAACCCTTCCG
GATTTCATTGGAGGGCTGGTGAAATTGAAACGACTAAATCTGGAGGGTAACCCGCTAATTCGACCACCGCCAGATATAGTTAGAGATGGTGTGGAAGCAATTAAGCAATACATGAGCAAAAGCGCCACCAAGAGACTCGTACCACGTAGCGTTTCAAGTCTGGGAGGCAAACTGGCACGTAAGTGGATATCTTGCATCAG
TGAAGCTGCGGGAATATGACATCTGGCAAAGTGATGACGGGGCAGAAATACATCTGTGAAGAAGAGGTTTTTAAGTCACGACTCTACTATGCAATCCTCGCCACGTGGATTCAACTAGCACTGATGACATACACAAGCTGCGTTGGGTTAATTCTCTTTTTCGATGGATTAGTCTATATTAGGATAATTGTTGATTTTAA
AATTTGATCAAATGCAAATACTCTTATTCTTAACCATCTATTATTATCCATAGTTGACCAAAATTTATAATGTTCAAGAAAAACAAATTAACACAG

>TRINITY_DN96916_c0_g1_i2 from sample A2 / Example unspliced isoform:

TCACAACAGGGCTATCCCTGTTGCAATCTTTCCAGACACAGCCAAGGTTCGAAACTCCGCAACAATGGCGTCGGCAAGGGCTGCAGCAGAGGAGGGAAGCCTCGGAACACGGCTGAGTCACGATCTTGTCAAAGGATCGCAGAAGGCCGTATCCCGCATTGAGTCAGAATTCGCATCGAAGATCAAAGCCATCGCAGCCC
TCCCCAACCGCCCCGACATGGATATCTCGCTCTGCAACACTGCCAAGGTTTCAATGGAGGCCGAGGCGAAGAGAATTGCAGAAGAAGAAAAGGCTCCGCACTTATCCGTTATACAGGTCAAGAAGTCCGAGAATCCACCAATCTCAAGAAGTACTGAAGCTGATATTCAGGGAAATATGAATCTAGACCTTCAACAGCTT
CTCCACGAACTTAGAAGGCTTCTACTCTTTAACTTGCCAGACTCTGCACAAAAGCTATCGACCATCGCTAAACTCGACCTTTCTAATAACAACATGAAGgtaagtgcagcaatattgtatcctgatatagactttcttgtttatttgattttgcttgtaatactatttcttgctgcagTGTCTTACCGAATCCGTGACAA
GTCTGGTGAATTTAAGAACTCTCGACGTCCATTCGAACCAGCTCACATCGCTGCCGCATTCCATGGGACGATTGCAGAAGCTTAAAATCCTCAATATTTCTGGAAACTTATTTGCCGAATTGCCAGAAAGTGTTCAAAATTGCCGTTCGCTGGAAGAGCTGATTGCTGATTTCAACAAGCTACAGCAGCTTCCTGAAACG
CTGGGCATCAAACTGTTTAACCTGCAGAAGCTCTCTGTTCATTCCAACAAACTTTCTTATCTCCCTTCTTCCATCTCATGCCTGATGTCTCTGCGTGTTTTGGACGTACATATGAACAAACTCGGATATCTGCCTGAAGACATTGACAACCTGGTAAGTCTTCAAATTCTTAATGTTAGTTGCAATTTTGGCTACTTGAA
CGCCTTGCCGGATTCGATCTGTGGGCTGTGTTCACTCGTTGAATTAGACGCGAGTTTTAACCAGATTAAAACCCTTCCGGATTTCATTGGAGGGCTGGTGAAATTGAAACGACTAAATCTGGAGGGTAACCCGCTAATTCGACCACCGCCAGATATAGTTAGAGATGGTGTGGAAGCAATTAAGCAATACATGAGCAAAA
GCGCCACCAAGAGACTCGTACCACGTAGCGTTTCAAGTCTGGGAGGCAAACTGGCACGTAAGTGGATATCTTGCATCAGTGAAGCTGCGGGAATATGACATCTGGCAAAGTGATGACGGGGCAGAAATACATCTGTGAAGAAGAGGTTTTTAAGTCACGACTCTACTATGCAATCCTCGCCACGTGGATTCAACTAGCAC
TGATGACATACACAAGCTGCGTTGGGTTAATTCTCTTTTTCGATGGATTAGTCTATATTAGGATAATTGTTGATTTTAAAATTTGATCAAATGCAAATACTCTTATTCTTAACCATCTATTATTATCCATAGTTGACCAAAATTTATAATGTTCAAGAAAAACAAATTAACACAG

Intron 3 GTCGAGTCAC ... TAATTTTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	25	3	2	0
A2	100	0	1	1
A3	50	4	7	1
A4	100	0	2	1
A5	-1	0	0	0
A6	0	1	0	0
A7	6.67	7	1	0
A8	55.56	2	3	2
A9	71.43	2	3	7
A10	61.11	7	15	7

>TRINITY_DN41126_c1_g1_i4 from sample A8 / Example spliced isoform

TATTAATGAAAATAATATCCACACCAGAATCTACTCGATAGAATGCTATTACGTTAACTCATCATTATATTCAACGAAAGTAGGTCGTATGTGGCTTTCTATTTACCAACTAACTTATGATAAACTTATCAACTCCGCCTAATCACAACAGGGCTATCCCTGTTGCAATCTTTCCAGACACAGCCAAGGTTCGAAACTCC
GCAACAATGGCGTCGGCGGGGGCAGCAGCAACGCTGCCAAGGCTGCAATGGTGACCGAGGCGAAGAGAATTGCAGAAGAAGAAAAGGCTCGACACATATCCGTTATACAGCTTGAGGAGCTCTTCCTCAAGCTGATTCCAACAATTTCAAGAACTGAAGCTGATATCCAGGGAAACATGAGGCTAGACCTTCAACAGCTT
CTCCATGAACTTAGAAAGCTTCTACTCTTTAACTTGCCAGACTCTGCACAAAAGCTATCGACCATCGCTAAACTCGACCTTTCTAATAACAACATGAAGTGTCTTACCGAATCCGTGACAAGTCTGGTGAATTTAAGAACTCTCGACGTCCATTCGAACCAGCTCACATCGCTGCCGCATTCCATGGGACGATTGCAGAA
GCTTAAAATCCTCAATATTTCTGGAAACTTATTTGCCGAATTGCCAGAAAGTGTTCAAAATTGCCGTTCGCTGGAAGAGCTGATTGCTGATTTCAACAAGCTACAGCAGCTTCCTGAAACGCTGGGCATCAAACTGTTTAACCTGCAGAAGCTCTCTGTTCATTCCAACAAACTTTCTTATCTCCCTTCTTCCATCTCAT
GCCTGATGTCTCTGCGTGTTTTGGACGTACATATGAACAAACTCGGATATCTGCCTGAAGACATTGACAACCTGGTAAGTCTTCAAATTCTTAATGTTAGTTGCAATTTTGGCTACTTGAACGCCTTGCCGGATTCGATCTGTGGGCTGTGTTCACTCGTTGAATTAGACGCGAGTTTTAACCAGATTAAAACCCTTCCG
GATTTCATTGGAGGGCTGGTGAAATTGAAACGACTAAATCTGGAGGGTAACCCGCTAATTCGACCACCGCCAGATATAGTTAGAGATGGTGTGGAAGCAATTAAGCAATACATGAGCAAAAGCGCCACCAAGAGACTCGTACCACGTAGCGTTTCAAGTCTGGGAGGCAAACTGGCACGTAAGTGGATATCTTGCATCAG
TGAAGCTGCGGGAATATGACATCTGGCAAAGTGATGACGGGGCAGAAATACATCTGTGAAGAAGAGGTTTTTAAGTCACGACTCTACTATGCAATCCTCGCCACGTGGATTCAACTAGCACTGATGACATACACAAGCTGCGTTGGGTTAATTCTCTTTTTCGATGGATTAGTCTATATTAGGATAATTGTTGATTTTAA
AATTTGATCAAATGCAAATACTCTTATTCTTAACCATCTATTATTATCCATAGTTGACCAAAATTTATAATGTTCAAGAAAAACAAATTAACACAGTAATCTATATTTAAACTCCTACAATTAAACACTTAAAACATTTTGGGATTTTATTTGTTTTTGAATAATATCAAAACATTTTAAGGTGTTATTTGTTTGTAAAT
AATATCGAGGTAGTTGAAGCACTCTCTCAAACCTACTTAATTCTTTTTCGTGGAAATTTATCTCCAAAATGGGGAAATATAATGGAAAAGCTTACAAGGTTAATGATGGCAAA

>TRINITY_DN41126_c1_g1_i17 from sample A8 / Example unspliced isoform:

TATTAATGAAAATAATATCCACACCAGAATCTACTCGATAGAATGCTATTACGTTAACTCATCATTATATTCAACGAAAGTAGGTCGTATGTGGCTTTCTATTTACCAACTAACTTATGATAAACTTATCAACTCCGCCTAATCACAACAGGGCTATCCCTGTTGCAATCTTTCCAGACACAGCCAAGGTTCGAAACTCC
GCAACAATGGCGTCGGCGGGGGCAGCAGCAACGCTGCCAAGgtcgagtcacctcagactcaattcttccttttctgaattttaatcttacttatttgcagtgtaaacaaattgcatcgattaattttcagGCTGCAATGGTGACCGAGGCGAAGAGAATTGCAGAAGAAGAAAAGGCTCGACACATATCCGTTATACAGC
TTGAGGAGCTCT

Intron 4 GTAAAACTCT ... AATTTATCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	45.45	9	6	9
A2	56.52	5	11	2
A3	46.67	4	3	4
A4	30.77	9	1	7
A5	33.33	5	2	3
A6	43.75	9	6	8
A7	45.1	28	21	25
A8	29.82	20	10	7
A9	24.32	14	6	3
A10	34.88	28	16	14

>TRINITY_DN41126_c1_g1_i13 from sample A8 / Example spliced isoform

AATCAATCTCACCAAATAATTGTGTTTGGTTGTTCACTATGGTGTTATCCCGTCTATCAATAATAGCAACAGAGGAAAAAATAACGTAACTGCAACTTCTCTCTTATATACTCGTCGTTATTAATGGATGGAATTTTTCTACATGATGTTGCAACATCGATGCTGATCGGCCATTTCTGTTTTATACCCTTGTTGTTATG
AATGGATGGAATAATTCCAAGGATAAACTGCAGCTCAGCCTTCAATTTCAAGAACACGTATCCTTTCTCTGACCAGATGATGTTGCATCATCGATGATGGTCGGCTTCTTGCCGTTTATGGTTCTGGACTTGGCACATTGCGTCTATTAATTCTCCGATTAATTTAGTATGGAAGGTGTAGGTAGGAAGAGTCTGAAAAG
ACTCTGCACCCTCATCTAACTAATAATTTCAATTGCTTCACGGCGAGGCGGCCATGAAAATAGACCTTCAACATTGCTTTTGGAATCTTTGAATGGAAGCCGACGCAACTGTGAAAGATTAATTAATGCAATATCCAAGGATCGAACCTCGGCTCTCTGACCCTGACACCGCTACAATGGACGAAAGCCTGGGAGCTCGG
CCGAGTCAGGGTCTTGTAAAAGAATCGCGGAAGGCCATAGCGCGCATTGAGTCGGAATTGGCAGGGAAGATCGAAGCCATCGCAGCCCTTCCCAATCCCCCCGGCATGGATATCTTAGTCTGCCAGGCTGCCAAGGCTAAGATGGAGGCCGGGGAGAAGAGAATTGCAGAAGCAAAAAAGGTTCCGCACAGATCCGTTAT
ACAGCTTGAGGAGCTCTATCAAACGTACGACGCCATTGTTATCAAGTCCGCATCAGCCATCGTGGACGACTCTGAGAATATTCCTGCAATCTCAAGAGCGGAAACTGATCTTGATCGAAATATGAAGCTAAATCCTGCAATCTCAAGAGCGGAAACTGATCTTGATCGAAATGTGAAGCTAAATCCTGCAATCTCAAGAG
CGGAAACTGATCTTGATCGAAATATGAAGCTAAATCCTGAACTGTTTCTGCAAGAACTGAAAGGGCTTATACTCTTTAACGTGCCAGACTCCTCACAGAAGCTCACGGCCATCGCTAAACTCGACCTTTCTAATAACAACATCAAGTGTCTGCCTGAATCCGTGACAAGTCTGGTGAATTTGAGAATTCTCGACGTTCAT
TCGAATCAGCTCACATCTTTACCCAAATCCATGGGACGGCTTCCTAACCTCAAAATCCTCAATATTTCTGGAAATCTCTTCACCGATTTGCCACAAAGCGTTCAAAATTGCGGTTCGCTGGAAGAGTTGATTGCGGATTTCAACAAGCTACAGCGGCTGCCTGAATCTCTGGGATTCAAACTCCTTAACCTGCAAAAGCT
TTCCGTTCATTCCAACGAACTTTGTGATCTTCCTCTCTCCATCTGCCACATGATGTGTCTGCGTGTTCTGGACGTCCATATGAACAAACTTAGATATCTACCCGAAGATATTGAAAACCTTGTGAGCCTTCAAATTCTTGACGTTAGTTGCAATTTCGACAACTTCAAAGCATTGCCGGATTCGATCTGTGGGCTGACTA
CTCTCGTGGCATTAGATGTGAGTTATAACCAGATTAAAACCCTACCCGATTTCATTGGAGGTCTCATGGAGCTGAAACGCCTAAATCTGGAGGGGAATCCGCTAATTCTACCACCGCCACAGATCGTTAGAGAAGGCCTCGAAGCGGTTATGAATTACATGAGAAATCGAGCCACACAATGTCTCCTGCCGCCTAGTTTG
TCATGCCTGTTAACCAAACAGCCACGTAAGTTGGTAGCTTGCGCAGGTGGAAACCGTGGGAGTAGAAACTTCAGTAGATACTTCCAAAAGCGATGACGTGGAACGAATGCAGATCTGAAGAGACGGCTTCTCGGCACCACCAGTTGTTTCACTTCTAGTGGCTACCATCAACACCGAGATGCACTCCCCGCCATAAGAAG
AACTAATGCCAACATTTTGCCAGCCTTATGCTTCTCAATTTCTCAAGCCTGCTACCATATAAAATGTTGAATTATTCTTTAAAGTTTGATCCATGAATGCACCTAGGAATTTAACTATTTATCCTCTCCAAATATATTATGTTTCTGTGTTATAAAGTTTTGAAGTCGCAAGTTTCTGAATGATTAAATGAATAAAAAGG
CTCTGTATTTTAGAAAAACGTGTTAAGTTTTCA

>TRINITY_DN41126_c1_g1_i5 from sample A8 / Example unspliced isoform:

AATCAATCTCACCAAATAATTGTGTTTGGTTGTTCACTATGGTGTTATCCCGTCTATCAATAATAGCAACAGAGGAAAAAATAACGTAACTGCAACTTCTCTCTTATATACTCGTCGTTATTAATGGATGGAATTTTTCTACATGATGTTGCAACATCGATGCTGATCGGCCATTTCTGTTTTATACCCTTGTTGTTATG
AATGGATGGAATAATTCCAAGGATAAACTGCAGCTCAGCCTTCAATTTCAAGAACACGTATCCTTTCTCTGACCAGATGATGTTGCATCATCGATGATGGTCGGCTTCTTGCCGTTTATGGTTCTGGACTTGGCACATTGCGTCTATTAATTCTCCGATTAATTTAGTATGGAAGGTGTAGGTAGGAAGAGTCTGAAAAG
ACTCTGCACCCTCATCTAACTAATAATTTCAATTGCTTCACGGCGAGGCGGCCATGAAAATAGACCTTCAACATTGCTTTTGGAATCTTTGAATGGAAGCCGACGCAACTGTGAAAGATTAATTAATGCAATATCCAAGGATCGAACCTCGGCTCTCTGACCCTGACACCGCTACAATGGACGAAAGCCTGGGAGCTCGG
CCGAGTCAGGGTCTTGTAAAAGAATCGCGGAAGGCCATAGCGCGCATTGAGTCGGAATTGGCAGGGAAGATCGAAGCCATCGCAGCCCTTCCCAATCCCCCCGGCATGGATATCTTAGTCTGCCAGGCTGCCAAGGCTAAGATGGAGGCCGGGGAGAAGAGAATTGCAGAAGCAAAAAAGGTTCCGCACAGATCCGTTAT
ACAGCTTGAGGAGCTCTATCAAACGTACGACGCCATTGTTATCAAGTCCGCATCAGCCATCGTGGACGACTCTGAGAATATTCCTGCAATCTCAAGAGCGGAAACTGATCTTGATCGAAATATGAAGCTAAATCCTGCAATCTCAAGAGCGGAAACTGATCTTGATCGAAATGTGAAGCTAAATCCTGCAATCTCAAGAG
CGGAAACTGATCTTGATCGAAATATGAAGCTAAATCCTGAACTGTTTCTGCAAGAACTGAAAGGGCTTATACTCTTTAACGTGCCAGACTCCTCACAGAAGCTCACGGCCATCGCTAAACTCGACCTTTCTAATAACAACATCAAGTGTCTGCCTGAATCCGTGACAAGTCTGGTGAATTTGAGAATTCTCGACGTTCAT
TCGAATCAGCTCACATCTTTACCCAAATCCATGGGACGGCTTCCTAACCTCAAAATCCTCAATATTTCTGGAAATCTCTTCACCGATTTGCCACAAAGCGTTCAAAATTGCGGgtaaaactctatttcgaatcattaatttcttccgcaaacatttaattctgcacggtttgaactttgctcaattgtatgattttgccg
ctaatagaaatttatcagTTCGCTGGAAGAGTTGATTGCGGATTTCAACAAGCTACAGCGGCTGCCTGAATCTCTGGGATTCAAACTCCTTAACCTGCAAAAGCTTTCCGTTCATTCCAACGAACTTTGTGATCTTCCTCTCTCCATCTGCCACATGATGTGTCTGCGTGTTCTGGACGTCCATATGAACAAACTTAGAT
ATCTACCCGAAGATATTGAAAACCTTGTGAGCCTTCAAATTCTTGACGTTAGTTGCAATTTCGACAACTTCAAAGCATTGCCGGATTCGATCTGCGGGCTGACTACTCTCGTGGCATTAGATGTGAGTTATAACCAGATTAAAACCCTACCCGATTTCATTGGAGGTCTCATGGAGCTGAAACGCCTAAATCTGGAGGGG
AATCCGCTAATTCTACCACCGCCACAGATCGTTAGAGAAGGCCTCGAAGCGGTTATGAATTACATGAGAAATCGAGCCACACAATGTCTCCTGCCGCCTAGTTTGTCATGCCTGTTAACCAAACAGCCACGTAAGTTGGTAGCTTGCGCAGGTGGAAACCGTGGGAGTAGAAACTTCAGTAGATACTTCCAAAAGCGATG
ACGTGGAACGAATGCAGATCTGAAGAGACGGCTTCTCGGCACCACCAGTTGTTTCACTTCTAGTGGCTACCATCAACACCGAGATGCACTCCCCGCCATAAGAAGAACTAATGTCAACATTTTAGCAGCCTTATGCTTCTCAATTTCTCAAGCCTGCTACCATATAAAATGTTGAATTATTCTTTAAAGTTTGATCCATG
AATGCACCTAGGAATTTAACTATTTATCCTCTCCAAATATATTATGTTTCTGTGTTATAAAGTTTTGAAGTCGCAAGTTTCTGAATGATTAAATGAATAAAAAGGCTCTGTATTTTAGAAAAACGTGTTAAGTTTTCA

Intron 5 GTTGACTCAC ... ATTAATGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	27.78	13	2	5
A2	100	0	2	3
A3	0	10	0	0
A4	63.64	4	0	7
A5	16.67	5	0	1
A6	9.09	20	2	2
A7	14.93	57	2	10
A8	8.33	33	1	3
A9	26.42	39	1	14
A10	22.06	53	1	15

>TRINITY_DN40625_c0_g2_i8 from sample A9 / Example spliced isoform

TATGGTGTTATCCCGTCTATCAATAATAGCAACAGAGGAAAAAATAACGTAACTGCAACTTCTCTCTTATATACTCGTCGTTATTAATGGATGGAATTTTTCTACATGATGTTGCAACATCGATGCTGATCGGCCATTTCTGTTTTATACCCTTGTTGTTATGAATGGATGGAATAATTCCAAGGATAAACTGCAGCTCA
GCCTTCAATTTCAAGAACACGTATCCTTTTTGACCAGATGATGTTGCATCATCGATGATGGTCGGCTTCTTGCCGTTTATGGTTCTGGACTTGGCACATTGCGTCTATTAATTGTCCGATGAATTTAGTATGGAAGGTGTTGGTAGGAAGAGTCTGAAAAGACTCTGCACCCTCATCTAACTAATAATTTCAATTGCTTC
ACGGCGAGGCGGCCATGAAAATAGACCTTCAACATTTCTTTTGGAATCTTTGAATGGAAGCCGACGCAACTGTGAAAGATTAATTAATGCAATATCCAAGGATCGAACCTGGCTCTCTGACCCTGACACCGCTACAATGGACGAAAGCCTGGGAGCTCGGCCGAGTCAGGGTCTTGTAAAAGAATCGCGGAAGGCCATAG
CGCGCATTGAGTCAGAATTGGCAGGGAAGATCGAAGCCATCGCAGCCCTTCCCAATCCCCCCGGCATGGATATCTTAGTCTGCCAGGCTGCCAAGGCTAAGATGGAGGCCGGGGAGAAGAGAATTGCAGAAGCAAAAAAGGTTCCGCACAGATCCGTTATACAGCTTGAGGAGCTCTATCAAACGTACGACGCCATTGTT
ATCAAGTCCGCATCAGCCATCGTGGACGACTATGAGAATATTCCTGCAATCTCAAGAGCGGAAACTGATCTTGATCGAAATATGAAGCTAAATCCTGCAATCTCAAGAGCGGAAACTGATCTTGATCGAAATGTGAAGCTAAATCCTGCAATCTCAAGAGCGGAAACTGATCTTGATCGAAATGTGAAGCTAAATCCTGC
AATCTCAAGAGCGGAAACTGATCTTGATCGAAATATGAAGCTAAATCCTGAACTGTTTCTGCAAGAACTGAAAGGGCTTATACTCTTTAACGTGCCAGACTCCTCACAGAAGCTCACGGCCATCGCTAAACTCGACCTTTCTAATAACAACAT

>TRINITY_DN40625_c0_g2_i4 from sample A9 / Example unspliced isoform:

CTTAGTCTGCCAGGCTGCCAAGgttgactcacctcagactcaattctttcttttgtgatttctattttcacttatttgcagtattagcaattgcattcattaattaatgcagGCTAAGATGGAGGCCGGGGAGAAGAGAATTGCAGAAGCAAAAAAGGTTCCGCACAGATCCGTTATACAGCTTGAGGAGCTCTATCAAA
CGTACGACGCCATTGTTATCAAGTCCGCATCAGCCATCGTGGACGACTATGAGAATATTCCTGCAATCTCAAGAGCGGAAACTGATCTTGATCGAAATATGAAGCTAAATCCTGCAATCTCAAGAGCGGAAACTGATCTTGATCGAAATGTGAAGCTAAATCCTGCAATCTCAAGAGCGGAAACTGATCTTGATCGAAAT
GTGAAGCTAAATCCTGCAATCTCAAGAGCGGAAACTGATCTTGATCGAAATATGAAGCTAAATCCTGAACTGTTTCTGCAAGAACTGAAAGGGCTTATACTCTTTAACGTGCCAGACTCCTCACAGAAGCTCACGGCCATCGCTAAACTCGACCTTTCTAATAACAACAT

Intron 6 GTAAGTCTTC ... TTTTAACCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	59.46	15	24	20
A2	56.36	12	22	9
A3	52	12	7	19
A4	52.63	9	13	7
A5	75	2	6	6
A6	57.51	41	50	61
A7	63.49	69	121	119
A8	61.37	90	120	166
A9	61.05	89	127	152
A10	62.5	57	103	87

>TRINITY_DN102513_c0_g2_i1 from sample A3 / Example spliced isoform

CTCCGCCTAATCACAACAGGGCTATCCCTGTTGCAATCTTTCCAGACACAGCCAAGGTTCGAAACTCCGCAACAATGGCGTCGGCAAGGGCTGCAGCAGAGGAGGGAAGCCTCGGAACACGGCTGAGTCACGATCTTGTCAAAGGATCGCAGAAGGCCGTATCCCGCATTGAGTCAGAATTCGCATCGAAGATCAAAGCC
ATCGCAGCCCTCCCCAACCGCCCCGACATGGATATCTCGCTCTGCAACACTGCCAAGGTTTCAATGGAGGCCGAGGCGAAGAGAATTGCAGAAGAAGAAAAGGCTCCGCACTTATCCGTTATACAGGTCAAGAAGTCCGAGAATCCACCAATCTCAAGAAGTACTGAAGCTGATATTCAGGGAAATATGAATCTAGACCT
TCAACAGCTTCTCCACGAACTTAGAAGGCTTCTACTCTTTAACTTGCCAGACTCTGCACAAAAGCTATCGACCATCGCTAAACTCGACCTTTCTAATAACAACATGAAGTGTCTTACCGAATCCGTGACAAGTCTGGTGAATTTAAGAACTCTCGACGTCCATTCGAACCAGCTCACATCGCTGCCGCATTCCATGGGAC
GATTGCAGAAGCTTAAAATCCTCAATATTTCTGGAAACTTATTTGCCGAATTGCCAGAAAGTGTTCAAAATTGCCGTTCGCTGGAAGAGCTGATTGCTGATTTCAACAAGCTACAGCAGCTTCCTGAAACGCTGGGCATCAAACTGTTTAACCTGCAGAAGCTCTCTGTTCATTCCAACAAACTTTCTTATCTCCCTTCT
TCCATCTCATGCCTGATGTCTCTGCGTGTTTTGGACGTACATATGAACAAACTCGGATATCTGCCTGAAGACATTGACAACCTGATTAAAACCCTTCCGGATTTCATTGGAGGGCTGGTGAAATTGAAACGACTAAATCTGGAGGGTAACCCGCTAATTCGACCACCGCCAGATATAGTTAGAGATGGTGTGGAAGCAAT
TAAGCAATACATGAGCAAAAGCGCCACCAAGAGACTCGTACCACGTAGCGTTTCAAGTCTGGGAGGCAAACTGGCACGTAAGTGGATATCTTGCATCAGTGAAGCTGCGGGAATATGACATCTGGCAAAGTGATGACGGGGCAGAAATACATCTGTGAAGAAGAGGTTTTTAAGTCACGACTCTACTATGCAATCCTCGC
CACGTGGATTCAACTAGCACTGATGACATACACAAGCTGCGTTGGGTTAATTCTCTTTTTCGATGGATTAGTCTATATTAGGATAATTGTTGATTTTAAAATTTGATCAAATGCAAATACTCTTATTCTTAACCATCTATTATTATCCATAGTTGACCG

>TRINITY_DN102513_c0_g2_i2 from sample A3 / Example unspliced isoform:

CTCCGCCTAATCACAACAGGGCTATCCCTGTTGCAATCTTTCCAGACACAGCCAAGGTTCGAAACTCCGCAACAATGGCGTCGGCAAGGGCTGCAGCAGAGGAGGGAAGCCTCGGAACACGGCTGAGTCACGATCTTGTCAAAGGATCGCAGAAGGCCGTATCCCGCATTGAGTCAGAATTCGCATCGAAGATCAAAGCC
ATCGCAGCCCTCCCCAACCGCCCCGACATGGATATCTCGCTCTGCAACACTGCCAAGGTTTCAATGGAGGCCGAGGCGAAGAGAATTGCAGAAGAAGAAAAGGCTCCGCACTTATCCGTTATACAGGTCAAGAAGTCCGAGAATCCACCAATCTCAAGAAGTACTGAAGCTGATATTCAGGGAAATATGAATCTAGACCT
TCAACAGCTTCTCCACGAACTTAGAAGGCTTCTACTCTTTAACTTGCCAGACTCTGCACAAAAGCTATCGACCATCGCTAAACTCGACCTTTCTAATAACAACATGAAGTGTCTTACCGAATCCGTGACAAGTCTGGTGAATTTAAGAACTCTCGACGTCCATTCGAACCAGCTCACATCGCTGCCGCATTCCATGGGAC
GATTGCAGAAGCTTAAAATCCTCAATATTTCTGGAAACTTATTTGCCGAATTGCCAGAAAGTGTTCAAAATTGCCGTTCGCTGGAAGAGCTGATTGCTGATTTCAACAAGCTACAGCAGCTTCCTGAAACGCTGGGCATCAAACTGTTTAACCTGCAGAAGCTCTCTGTTCATTCCAACAAACTTTCTTATCTCCCTTCT
TCCATCTCATGCCTGATGTCTCTGCGTGTTTTGGACGTACATATGAACAAACTCGGATATCTGCCTGAAGACATTGACAACCTGgtaagtcttcaaattcttaatgttagttgcaattttggctacttgaacgccttgccggattcgatctgtgggctgtgttcactcgttgaattagacgcgagttttaaccagATTAA
AACCCTTCCGGATTTCATTGGAGGGCTGGTGAAATTGAAACGACTAAATCTGGAGGGTAACCCGCTAATTCGACCACCGCCAGATATAGTTAGAGATGGTGTGGAAGCAATTAAGCAATACATGAGCAAAAGCGCCACCAAGAGACTCGTACCACGTAGCGTTTCAAGTCTGGGAGGCAAACTGGCACGTAAGTGGATAT
CTTGCATCAGTGAAGCTGCGGGAATATGACATCTGGCAAAGTGATGACGGGGCAGAAATACATCTGTGAAGAAGAGGTTTTTAAGTCACGACTCTACTATGCAATCCTCGCCACGTGGATTCAACTAGCACTGATGACATACACAAGCTGCGTTGGGTTAATTCTCTTTTTCGATGGATTAGTCTATATTAGGATAATTG
TTGATTTTAAAATTTGATCAAATGCAAATACTCTTATTCTTAACCATCTATTATTATCCATAGTTGACCG