NA ----> NA

Intron 1 GTAAATACTT ... TGAATTATAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	75	16	52	44
A2	75.28	11	36	31
A3	82.22	8	42	32
A4	84.85	10	59	53
A5	75	6	14	22
A6	76.92	15	53	47
A7	78.03	29	103	103
A8	75.82	33	109	98
A9	70.67	22	53	53
A10	81.46	14	72	51

>TRINITY_DN44678_c0_g2_i11 from sample A8 / Example spliced isoform

ATCTAAAGCTTCCTATGTGTTTAGATTTATTAAAACATCTTTCTCACCCTAGCTGCAATGAAGTGGTGCGGGTGGGGTTCTTACATTAAACCATGTATTCGTTTTTTTGGAGCTGAGGCAGATTGACTCCCAGGTGGTAATCAACTGGCTTAATCATATTGGGAGTATCAATGTTCTTGTGCTCTCTCCTTGGCTTGACA
AGATCAGATCTTTACAGTCTCACTTTCTGGGCATCCAATTTCTGCATGTGCCTTGGCTTAATCATATTGGGAGTATCAATGTTCTTGTGCTCTCTCCTTGGCTTGACAAGAACAGATCTTTACAGTCTCACTTTCCGGGCATCCAATTTATGCATGTGCCTCGGGAGGAAAATGGTCTAGCCGTCACTCTCTGGAAGCGT
GCTCTTTTATCGTTGGAAGGGATGCTGTTT

>TRINITY_DN44678_c0_g2_i10 from sample A8 / Example unspliced isoform:

TTCTCTGGATATATTCTTGGAAACTTCAGAGTGGCTTTCTGGTCTTCCTTCAGGTGAGAGTGAGAAGATGATTCATGATACCAGAGCTGCATTACTATCGGATTGCGGAGCATTTTATCTAAAGCTTCCTATGTGTTTAGATTTATTAAAACATCTTTCTCACCCTAGCTGCAATGAAGTGGTGCGGGTGGGGTTCTTAC
ATTAAACCATGgtaaatactttaaactatggatgaatttgggctcggggactaatactaaaagtgaactctgtgcattttgaattatagTATTCGTTTTTTTGGAGCTGAGGCAGATTGACTCCCAGGTGGTAATCAACTGGCTTAATCATATTGGGAGTATCAATGTTCTTGTGCTCTCTCCTTGGCTTGACAAGATCA
GATCTTTACAGTCTCACTTTCTGGGCATCCAATTTCTGCATGTGCCTTGGCTTAATCATATTGGGAGTATCAATGTTCTTGTGCTCTCTCCTTGGCTTGACAAGAACAGATCTTTACAGTCTCACTTTCCGGGCATCCAATTTATGCATGTGCCTCGGGAGGAAAATGGTCTAGCCGTCACTCTCTGGAAGCGTGCTCTT
TTATCGTTGGAAGGGATGCTGTTT

Intron 2 GTGGGGTTCT ... ATTTTGCTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	69.05	13	23	35
A2	70.49	9	12	31
A3	76.67	7	16	30
A4	79.75	8	23	40
A5	68.42	3	1	12
A6	73.33	16	27	61
A7	78.61	20	44	103
A8	74.78	29	45	127
A9	70.86	22	20	87
A10	78	11	24	54

>TRINITY_DN44678_c0_g2_i3 from sample A8 / Example spliced isoform

CTAGCTGCAATGAAGTGGTGCAGTATTCGTCTTTTGGAGCTGAGGCAGATTACGGGTGACTCCCAGGTGGTAATCAACTGGCTTAATCATATTGGGAGTATCAATGTTCTTGTGCTCTCTCCTTGGCTTGACAAGATCAGATCTTTACAGTCTCACTTTCTGGGCATCCAATTTCTGCATGTGCCTTGGCTTAATCATAT
TGGGAGTATCAATGTTCTTGTGCTCTCTCCTTGGCTTGACAAGATCAGATCTTTACAGTCTCACTTTCTGGGCATCCAATTTCTGCATGTGCCTCGGGAGGAAAATGGTCTAGCCGTCACTCTCTGGAAGCGTGCTCTTTTATCGTTGGAAGGGATGCTGTTT

>TRINITY_DN44678_c0_g2_i4 from sample A8 / Example unspliced isoform:

ATCTAAAGCTTCCTATGTGTTTAGATTTATTAAAACATCTTTCTCACCCTAGCTGCAATGAAGTGGTGCAGgtggggttcttacattagaccatagaccatggtaaatactttaaactatggataaatttgggctcggggactaataccaaaagtgaattctgtgtattttggtttatcctttattttgctagTATTCGT
CTTTTGGAGCTGAGGCAGATTACGGGTGACTCCCAGGTGGTAATCAACTGGCTTAATCATATTGGGAGTATCAATGTTCTTGTGCTCTCTCCTTGGCTTGACAAGATCAGATCTTTACAGTCTCACTTTCTGGGCATCCAATTTCTGCATGTGCCTTGGCTTAATCATATTGGGAGTATCAATGTTCTTGTGCTCTCTCC
TTGGCTTGACAAGATCAGATCTTTACAGTCTCACTTTCTGGGCATCCAATTTCTGCATGTGCCTCGGGAGGAAAATGGTCTAGCCGTCACTCTCTGGAAGCGTGCTCTTTTATCGTTGGAAGGGATGCTGTTT

Intron 3 GTAAATACTT ... TGACTCCCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	43.35	66	29	72
A2	41.44	53	20	55
A3	39.46	79	22	81
A4	43.97	72	29	84
A5	37.5	35	6	36
A6	40.34	139	37	151
A7	44.28	168	79	188
A8	47.04	143	65	189
A9	42.73	126	51	137
A10	39.77	106	39	101

>TRINITY_DN44678_c0_g2_i13 from sample A8 / Example spliced isoform

ATCTAAAGCTTCCTATGTGTTTAGATTTATTAAAACATCTTTCTCACCCTAGCTGCAATGAAGTGGTGCAGGTGGGGTTCTTACATTAGACCATAGACCATGGTGGTAATCAACTGGCTTAATCATATTGGGAGTATCAATGTTCTTGTGCTCTCTCCTTGGCTTGACAAGATCAGATCTTTACAGTCTCACTTTCTGGG
CATCCAATTTCTGCATGTGCCTTGGCTTAATCATATTGGGAGTATCAATGTTCTTGTGCTCTCTCCTTGGCTTGACAAGATCAGATCTTTACAGTCTCACTTTCTGGGCATCCAATTTCTGCATGTGCCTCGGGAGGAAAATGGTCTAGCCGTCACTCTCTGGAAGCGTGCTCTTTTATCGTTGGAAGGGATGCTGTTT

>TRINITY_DN44678_c0_g2_i4 from sample A8 / Example unspliced isoform:

ATCTAAAGCTTCCTATGTGTTTAGATTTATTAAAACATCTTTCTCACCCTAGCTGCAATGAAGTGGTGCAGGTGGGGTTCTTACATTAGACCATAGACCATGgtaaatactttaaactatggataaatttgggctcggggactaataccaaaagtgaattctgtgtattttggtttatcctttattttgctagtattcgt
cttttggagctgaggcagattacgggtgactcccagGTGGTAATCAACTGGCTTAATCATATTGGGAGTATCAATGTTCTTGTGCTCTCTCCTTGGCTTGACAAGATCAGATCTTTACAGTCTCACTTTCTGGGCATCCAATTTCTGCATGTGCCTTGGCTTAATCATATTGGGAGTATCAATGTTCTTGTGCTCTCTCC
TTGGCTTGACAAGATCAGATCTTTACAGTCTCACTTTCTGGGCATCCAATTTCTGCATGTGCCTCGGGAGGAAAATGGTCTAGCCGTCACTCTCTGGAAGCGTGCTCTTTTATCGTTGGAAGGGATGCTGTTT

Intron 4 GTAAATACTT ... TGACTCCCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	44.48	83	52	81
A2	37.66	72	36	51
A3	39.3	95	42	81
A4	46.83	88	59	96
A5	42.86	40	14	46
A6	37.07	163	53	139
A7	43.68	196	103	201
A8	43.82	184	109	178
A9	36.97	156	53	130
A10	39.32	142	72	112

>TRINITY_DN44678_c0_g2_i14 from sample A8 / Example spliced isoform

TTCTCTGGATATATTCTTGGAAACTTCAGAGTGGCTTTCTGGTCTTCCTTCAGGTGAGAGTGAGAAGATGATTCATGATACCAGAGCTGCATTACTATCGGATTGCGGAGCATTTTATCTAAAGCTTCCTATGTGTTTAGATTTATTAAAACATCTTTCTCACCCTAGCTGCAATGAAGTGGTGCGGGTGGGGTTCTTAC
ATTAAACCATGGTGGTAATCAACTGGCTTAATCATATTGGGAGTATCAATGTTCTTGTGCTCTCTCCTTGGCTTGACAAGATCAGATCTTTACAGTCTCACTTTCTGGGCATCCAATTTCTGCATGTGCCTTGGCTTAATCATATTGGGAGTATCAATGTTCTTGTGCTCTCTCCTTGGCTTGACAAGAACAGATCTTTA
CAGTCTCACTTTCCGGGCATCCAATTTATGCATGTGCCTCGGGAGGAAAATGGTCTAGCCGTCACTCTCTGGAAGCGTGCTCTTTTATCGTTGGAAGGGATGCTGTTT

>TRINITY_DN44678_c0_g2_i10 from sample A8 / Example unspliced isoform:

TTCTCTGGATATATTCTTGGAAACTTCAGAGTGGCTTTCTGGTCTTCCTTCAGGTGAGAGTGAGAAGATGATTCATGATACCAGAGCTGCATTACTATCGGATTGCGGAGCATTTTATCTAAAGCTTCCTATGTGTTTAGATTTATTAAAACATCTTTCTCACCCTAGCTGCAATGAAGTGGTGCGGGTGGGGTTCTTAC
ATTAAACCATGgtaaatactttaaactatggatgaatttgggctcggggactaatactaaaagtgaactctgtgcattttgaattatagtattcgtttttttggagctgaggcagattgactcccagGTGGTAATCAACTGGCTTAATCATATTGGGAGTATCAATGTTCTTGTGCTCTCTCCTTGGCTTGACAAGATCA
GATCTTTACAGTCTCACTTTCTGGGCATCCAATTTCTGCATGTGCCTTGGCTTAATCATATTGGGAGTATCAATGTTCTTGTGCTCTCTCCTTGGCTTGACAAGAACAGATCTTTACAGTCTCACTTTCCGGGCATCCAATTTATGCATGTGCCTCGGGAGGAAAATGGTCTAGCCGTCACTCTCTGGAAGCGTGCTCTT
TTATCGTTGGAAGGGATGCTGTTT