NA ----> NA

Intron 1 GTCATTTTAT ... TGAATCGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	68.42	3	2	11
A2	80	1	1	7
A3	63.64	10	18	17
A4	37.14	11	11	2
A5	100	0	0	4
A6	67.35	8	14	19
A7	56.52	20	22	30
A8	67.09	13	26	27
A9	62.03	15	28	21
A10	66.2	12	19	28

>TRINITY_DN37192_c1_g2_i2 from sample A6 / Example spliced isoform

TCTCACCTGGTTGTTGCGAAGATTTCATCGAAAAATCCTGATCAAAGAGAGTCCCCGATGCAACACGTATTACAGGAAGATTCGTTCCAAAAAATAATTAGAAACCATCAAACTGTGGCAGCTATCAAGATTACTCTCCTGTTCAGATTGCAAAGATATTGGAAGGCAGAGATTATGAACGCATAGTCGATGGAACAAGA
TGAGATATCCCACTTATCTGTGAAAGATGGTTCATAGTTTTAAAGAATATTTGTAGGATATGTCTGGTCTAGATTTCATGCACCAGTTGGAAGAGGCGAATTTCGAGCTCCATTTGTGAACTAACCATGACGAATCCTCTCAAACTGTCAAGAACCGACTTCCCAAATTAAGTCCTTTACCCCTACCCGCGGGCCGTTCG
TTAAATATCTCAACCGAAGTGGAGAAATTGGTGCCCGAACAGAGGTGTACTTTCAACATTTGGTTGCGGCCGGTTTGGGATATGGAGGAACGAACCAAAGTTTTTGGAAACGGATTCTATCATTCTTGCTTTTCAATTGTATAAATCTGACCTCAGAGGTCTGAGACCAAGGTCTCTGGAGATTGGAGAATGATTGTGTT
CTTCTCTGAAAAATCTCTTATTATTTTTTAAATGTTCTTCAGAAGAACTGAGGTGGACTCATTGTTGCTGTCAGATCGAGACTTGAATGGTTCGATAGGATAGGAGTATGATTGAAAAGACTGTACATTGGAGAAAATTCATAAACGTGATGCAAGAATCAGTTGGAATTTCGTTCCTGTGATTTTGTTAAGCACTTGTT
CTGGAACTGGTATCATTTTGAGTTTAGTTTTATGAATACATAAATATATTATCTATATTAACTTTCTTCTCC

>TRINITY_DN37192_c1_g2_i1 from sample A6 / Example unspliced isoform:

TCTCACCTGGTTGTTGCGAAGATTTCATCGAAAAATCCTGATCAAAGAGAGTCCCCGATGCAACACGTATTACAGGAAGATTCGTTCCAAAAAATAATTAGAAACCATCAAACTGTGGCAGCTATCAAGATTACTCTCCTgtcattttatctcaattttttttctttaagtttagttattgcttttgattttatttttag
aaattctaaattaataatgaatttgttgaatcgcagGTTCAGATTGCAAAGATATTGGAAGGCAGAGATTATGAACGCATAGTCGATGGAACAAGATGAGATATCCCACTTATCTGTGAAAGATGGTTCATAGTTTTAAAGAATATTTGTAGGATATGTCTGGTCTAGATTTCATGCACCAGTTGGAAGAGGCGAATTTC
GAGCTCCATTTGTGAACTAACCATGACGAATCCTCTCAAACTGTCAAGAACCGACTTCCCAAATTAAGTCCTTTACCCCTACCCGCGGGCCGTTCGTTAAATATCTCAACCGAAGTGGAGAAATTGGTGCCCGAACAGAGGTGTACTTTCAACATTTGGTTGCGGCCGGTTTGGGATATGGAGGAACGAACCAAAGTTTT
TGGAAACGGATTCTATCATTCTTGCTTTTCAATTGTATAAATCTGACCTCAGAGGTCTGAGACCAAGGTCTCTGGAGATTGGAGAATGATTGTGTTCTTCTCTGAAAAATCTCTTATTATTTTTTAAATGTTCTTCAGAAGAACTGAGGTGGACTCATTGTTGCTGTCAGATCGAGACTTGAATGGTTCGATAGGATAGG
AGTATGATTGAAAAGACTGTACATTGGAGAAAATTCATAAACGTGATGCAAGAATCAGTTGGAATTTCGTTCCTGTGATTTTGTTAAGCACTTGTTCTGGAACTGGTATCATTTTGAGTTTAGTTTTATGAATACATAAATATATTATCTATATTAACTTTCTTCTCC

Intron 2 GTAAATTGAA ... TGAATCGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	84.62	2	0	11
A2	87.5	1	1	7
A3	73.91	6	1	17
A4	66.67	1	0	2
A5	100	0	0	4
A6	86.36	3	0	19
A7	73.17	11	1	30
A8	75	9	12	27
A9	72.41	8	4	21
A10	73.68	10	0	28

>TRINITY_DN39792_c0_g1_i2 from sample A9 / Example spliced isoform

GAAAGAACAAAGCATTAAAGAAGAGATCGCTAGATTTTGAGCACTGATCCGACACCTGTGAATTGCTTGAAGTAAAAGTCATCTCACCAATCTGACAAGCAAGAATGATTATGCCACGATTTGTCGAAGAGATAAGCTCCAACAATCCTGGTTCAGATTGCAAAGATATTGGAAGGCAGAGATTATGAACGCATAGTCGA
TGGAACAAGATGAGATATCCCACTTATCTGTGAAAGATGGTTCATAGTTTTAAAGAATATTTGTAGGATATGTCTGGTCTAGATTTCATGCACCAGTTGGAAGAGGCGAATTTCGAGCTCCATTTGTGAACTAACCATGACGAATCCTCTCAAACTGTCAAGAACCGACTTCCCAAATTAAGTCCTTTACCCCTACCCGC
GGGCCGTTCGTTAAATATCTCAACCGAAGTGGAGAAATTGGTGCCCGAACAGAGGTGTACTTTCAACATTTGGTTGCGGCCGGTTTGGGATATGGAGGAACGAACCAAAGTTTTTGGAAACGGATTCTATCATTCTTGCTTTTCAATTGTATAAATCTGACCTCAGAGGTCTGAGACCAAGGTCTCTGGAGATTGGAGAA
TGATTGTGTTCTTCTCTGAAAAATCTCTTATTATTTTTTAAATGTTCTTCAGAAGAACTGAGGTGGACTCATTGTTGCTGTCAGATCGAGACTTGAATGGTTCGATAGGATAGGAGTATGATTGAAAAGACTGTACATTGGAGAAAATTCATAAACGTGATGCAAGAATCAGTTGGAATTTCGTTCCTGTGATTTTGTTA
AGCACTTGTTCTGGAACTGGTATCATTTTGAGTTTAGTTTTATGAATACATAAATATATTTTCTATATTAACTTTATTCTCC

>TRINITY_DN39792_c0_g1_i4 from sample A9 / Example unspliced isoform:

GAAAGAACAAAGCATTAAAGAAGAGATCGCTAGATTTTGAGCACTGATCCGACACCTGTGAATTGCTTGAAGTAAAAGTCATCTCACCAATCTGACAAGCAAGAATGATTATGCCACGATTTGTCGAAGAGATAAGCTCCAACAATCCTGgtaaattgaaaaaaatctaattgacgaagatgccacgtgaagcaagtttt
tgcagaagcagtttagttccataattacatcaaaaagatcagttaacaagtttgagatcatttgggatcgtattatctcacctggttgttgcgaagatttcatcgaaaaatcctgatcaaagagagtccccgatgcaacacgtattacaggaagattcgttccaaaaaataattagaaaccatcaaactgtggcagctat
caagattactctcctgtcattttatctcaattttttttctttaagtttagttattgcttttgattttatttttagaaattctaaattaataatgaatttgttgaatcgcagGTTCAGATTGCAAAGATATTGGAAGGCAGAGATTATGAACGCATAGTCGATGGAACAAGATGAGATATCCCACTTATCTGTGAAAGATG
GTTCATAGTTTTAAAGAATATTTGTAGGATATGTCTGGTCTAGATTTCATGCACCAGTTGGAAGAGGCGAATTTCGAGCTCCATTTGTGAACTAACCATGACGAATCCTCTCAAACTGTCAAGAACCGACTTCCCAAATTAAGTCCTTTACCCCTACCCGCGGGCCGTTCGTTAAATATCTCAACCGAAGTGGAGAAATT
GGTGCCCGAACAGAGGTGTACTTTCAACATTTGGTTGCGGCCGGTTTGGGATATGGAGGAACGAACCAAAGTTTTTGGAAACGGATTCTATCATTCTTGCTTTTCAATTGTATAAATCTGACCTCAGAGGTCTGAGACCAAGGTCTCTGGAGATTGGAGAATGATTGTGTTCTTCTCTGAAAAATCTCTTATTATTTTTT
AAATGTTCTTCAGAAGAACTGAGGTGGACTCATTGTTGCTGTCAGATCGAGACTTGAATGGTTCGATAGGATAGGAGTATGATTGAAAAGACTGTACATTGGAGAAAATTCATAAACGTGATGCAAGAATCAGTTGGAATTTCGTTCCTGTGATTTTGTTAAGCACTTGTTCTGGAACTGGTATCATTTTGAGTTTAGTT
TTATGAATACATAAATATATTTTCTATATTAACTTTATTCTCC