sp|Q8RWE5|PIRL8_ARATH ----> Plant intracellular Ras-group-related LRR protein 8

Intron 1 GTAGATGCAG ... TTTCATGTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	54.84	7	9	8
A2	48.15	7	4	9
A3	36.84	6	5	2
A4	53.85	3	4	3
A5	80	1	3	5
A6	31.82	15	3	11
A7	20.81	59	13	18
A8	30.36	39	10	24
A9	26.42	39	8	20
A10	15	34	8	4

>TRINITY_DN43864_c1_g4_i10 from sample A7 / Example spliced isoform

CAATAATAGCAACAGAGGAAAAAATAACGTAACTGCAACTTCTCTCTTATATACTCGTCGTTATTAATGGATGGAATTTTTCTACATGATGTTGCAACATCGATGCTGATCGGCCATTTCTGTTTTATACCCTTGTTGTTATGAATGGATGGAATAATTCCAAGGATAAACTGCAGCTCAGCCTTCAATTTCAAGAACAC
GTATCCTTTCTCTGACCAGATGATGTTGCATCATCGATGATGGTCGGCTTCTTGCCGTTTATGGTTCTGGACTTGGCACATTGCGTCTATTAATTCTCCGATTAATTTAGTATGGAAGGTGTAGGTAGGAAGAGTCTGAAAAGACTCTGCACCCTCATCTAACTAATAATTTCAATTGCTTCACGACGAGGCGGCCATGA
AAATAGACCTTCAACATTTCTTTTGGAATCTTTGAATGGAAGCCGACGCAACTGTGAAAGATTAATTAATGCAATATCCAAGGATCGAACCTCGGCTCTCTGACCCTGACACCGCTACAATGGACGAAAGCCTGGGAGCTCGGCCGAGTCAGGGTCTTGTAAAAGAATCGCGGAAGGCCATAGCGCGCATTGAGTCGGAA
TTGGCAGGGAAGATCGAAGCCATCGCAGCCCTTCCCAATCCCCCCGGCATGGATATCTTAGTCTGCCAGGCTGCCAAGGCTAAGATGGAGGCCGGGGAGAAGAGAATTGCAGAAGCAAAAAAGGTTCCGCACAGATCCGTTATACAGCTTGAGGAGCTCTATCAAACGTACGACGCCATTGTTATCAAGTCCGCATCAGC
CATCGTGGACGACTATGAGAATATTCCTGCAATCTCAAGAGCGGAAACTGATCTTGATCGAAATGTGAAGCTAAATCCTGCAATCTCAAGAGCGGAAACTGATCTTGATCGAAATATGAAGCTAAATCCTGAACTGTTTCTGCAAGAACTGAAAGGGCTTATACTCTTTAACGTGCCAGACTCCTCACAGAAGCTCACGG
CCATCGCTAAACTCGACCTTTCTAATAACAACATCAAGTGTCTGCCTGAATCCGTGACAAGTCTGGTGAATTTGAGAATTCTCGACGTTCATTCGAATCAGCTCACATCTTTACCCAAATCCATGGGACGGCTTCCTAACCTCAAAATCCTCAATATTTCTGGAAATCTCTTCACCGATTTGCCACAAAGCGTTCAAAAT
TGCGGTTCGCTGGAAGAGTTGATTGCGGATTTCAACAAGCTACAGCGGCTGCCTGAATCTCTGGGATTCAAACTCCTTAACCTGCAAAAGCTTTCCGTTCATTCCAACGAACTTTGTGATCTTCCTCTCTCCATCTGCCACATGATGTGTCTGCGTGTTCTGGACGTCCATATGAACAAACTTAGATATCTACCCGAAGA
TATTGAAAACCTTGTGAGCCTTCAAATTCTTGACGTTAGTTGCAATTTCGACAACTTCAAAGCATTGCCGGATTCGATCTGCGGGCTGACTACACTCGTGGCATTAGATGTGAGTTATAACCAGATTAAAACCCTACCCGATTTCATTGGAGGTCTCATGGAGCTGAAACGCCTAAATCTGGAGGGGAATCCGCTAATTC
TACCACCGCCACAGATCGTTAGAGAAGGCCTCGAAGCGGTTATGAATTACATGAGAAATCGAGCCACACAATGTCTCCTGCCGCCTAGTTTGTCATGCCTGTTAACCAAACAGCCACGTAAGTTGGTAGCTTGCGCAGGTGGAAACCGTGGGAGTAGAAACTTCAGTAGATACTTCCAAAAGCGATGACGTGGAACGAAT
GCAGATCTGAAGAGACGGCTTCTCGGCACCACCAGTTGTTGCACTTCTAGTGGCTACCATCAACACCGAGATGCACTCCCCGCCATAAGAAGAACTAATGCCAACATTTTGCCAGCCTTATGCTTCTCAATTTCTCAAGCCTGCTACCATATAAAATGTTGAATTATTCTTTAAAGTTTGATCCATGAATGCACCTAGGA
ATTTAACTATTTATCCTCTCCAAATATATTATGTTTCTGTGTTATAAAGTTTTGAAGTCGCAAGTTTCTGAATGATTAAATGAATAAAAAGGCTCTGTATTTTAGAAAAACGTGTTAAGTTTTCAGTTGTTCTTACATCACATTTTTCTGTATTATTTTTTACAAATTATATTGTATTATTTTTTACAAATTAT

>TRINITY_DN43864_c1_g4_i2 from sample A7 / Example unspliced isoform:

CAATAATAGCAACAGAGGAAAAAATAACGTAACTGCAACTTCTCTCTTATATACTCGTCGTTATTAATGGATGGAATTTTTCTACATGATGTTGCAACATCGATGCTGATCGGCCATTTCTGTTTTATACCCTTGTTGTTATGAATGGATGGAATAATTCCAAGGATAAACTGCAGCTCAGCCTTCAATTTCAAGAACAC
GTATCCTTTCTCTGACCAGATGATGTTGCATCATCGATGATGGTCGGCTTCTTGCCGTTTATGGTTCTGGACTTGGCACATTGCGTCTATTAATTCTCCGATTAATTTAGTATGGAAGGTGTAGGTAGGAAGAGTCTGAAAAGACTCTGCACCCTCATCTAACTAATAATTTCAATTGCTTCACGACGAGGCGGCCATGA
AAATAGACCTTCAACATTTCTTTTGGAATCTTTGAATGGAAGCCGACGCAACTGTGAAAGATTAATTAATGCAATATCCAAGGATCGAACCTCGGCTCTCTGACCCTGACACCGCTACAATGGACGAAAGCCTGGGAGCTCGGCCGAGTCAGGGTCTTGTAAAAGAATCGCGGAAGGCCATAGCGCGCATTGAGTCGGAA
TTGGCAGGGAAGATCGAAGCCATCGCAGCCCTTCCCAATCCCCCCGGCATGGATATCTTAGTCTGCCAGGCTGCCAAGGCTAAGATGGAGGCCGGGGAGAAGAGAATTGCAGAAGCAAAAAAGGTTCCGCACAGATCCGTTATACAGCTTGAGGAGCTCTATCAAACGTACGACGCCATTGTTATCAAGTCCGCATCAGC
CATCGTGGACGACTATGAGAATATTCCTGCAATCTCAAGAGCGGAAACTGATCTTGATCGAAATGTGAAGCTAAATCCTGCAATCTCAAGAGCGGAAACTGATCTTGATCGAAATATGAAGCTAAATCCTGAACTGTTTCTGCAAGAACTGAAAGGGCTTATACTCTTTAACGTGCCAGACTCCTCACAGAAGCTCACGG
CCATCGCTAAACTCGACCTTTCTAATAACAACATCAAGgtagatgcagtattacatatactttagtgcaggaaagaaaatcttttgtgattttgctaataacttatgaatttcttgtagtgtctgcccgaacactatagtgcaggaaataatataaatcttttgtgattttgctaataacttgatgaatttcatgtagTG
TCTGCCTGAATCCGTGACAAGTCTGGTGAATTTGAGAATTCTCGACGTTCATTCGAATCAGCTCACATCTTTACCCAAATCCATGGGACGGCTTCCTAACCTCAAAATCCTCAATATTTCTGGAAATCTCTTCACCGATTTGCCACAAAGCGTTCAAAATTGCGGTTCGCTGGAAGAGTTGATTGCGGATTTCAACAAGC
TACAGCGGCTGCCTGAATCTCTGGGATTCAAACTCCTTAACCTGCAAAAGCTTTCCGTTCATTCCAACGAACTTTGTGATCTTCCTCTCTCCATCTGCCACATGATGTGTCTGCGTGTTCTGGACGTCCATATGAACAAACTTAGATATCTACCCGAAGATATTGAAAACCTTGTGAGCCTTCAAATTCTTGACGTTAGT
TGCAATTTCGACAACTTCAAAGCATTGCCGGATTCGATCTGTGGGCTGACTACTCTCGTGGCATTAGATGTGAGTTATAACCAGATTAAAACCCTACCCGATTTCATTGGAGGTCTCATGGAGCTGAAACGCCTAAATCTGGAGGGGAATCCGCTAATTCTACCACCGCCACAGATCGTTAGAGAAGGCCTCGAAGCGGT
TATGAATTACATGAGAAATCGAGCCACACAATGTCTCCTGCCGCCTAGTTTGTCATGCCTGTTAACCAAACAGCCACGTAAGTTGGTAGCTTGCGCAGGTGGAAACCGTGGGAGTAGAAACTTCAGTAGATACTTCCAAAAGCGATGACGTGGAACGAATGCAGATCTGAAGAGACGGCTTCTCGGCACCACCAGTTGTT
GCACTTCTAGTGGCTACCATCAACACCGAGATGCACTCCCCGCCATAAGAAGAACTAATGTCAACATTTTAGCAGCCTTATGCTTCTCAATTTCTCAAGCCTGCTACCATATAAAATGTTGAATTATTCTTTAAAGTTTGATCCATGAATGCACCTAGGAATTTAACTATTTATCCTCTCCAAATATATTATGTTTCTGT
GTTATAAAGTTTTGAAGTCGCAAGTTTCTGAATGATTAAATGAATAAAAAGGCTCTGTATTTTAGAAAAACGTGTTAAGTTTTCAGTTGTTCTTACATCACATTTTTCTGTATTATTTTTTACAAATTATATTGTATTATTTTTTACAAATTAT

Intron 2 GTAAAACTCT ... AATTTATCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	45.45	9	6	9
A2	56.52	5	11	2
A3	46.67	4	3	4
A4	30.77	9	1	7
A5	33.33	5	2	3
A6	43.75	9	6	8
A7	45.1	28	21	25
A8	29.82	20	10	7
A9	24.32	14	6	3
A10	34.88	28	16	14

>TRINITY_DN43864_c1_g4_i1 from sample A7 / Example spliced isoform

CAATAATAGCAACAGAGGAAAAAATAACGTAACTGCAACTTCTCTCTTATATACTCGTCGTTATTAATGGATGGAATTTTTCTACATGATGTTGCAACATCGATGCTGATCGGCCATTTCTGTTTTATACCCTTGTTGTTATGAATGGATGGAATAATTCCAAGGATAAACTGCAGCTCAGCCTTCAATTTCAAGAACAC
GTATCCTTTCTCTGACCAGATGATGTTGCATCATCGATGATGGTCGGCTTCTTGCCGTTTATGGTTCTGGACTTGGCACATTGCGTCTATTAATTCTCCGATTAATTTAGTATGGAAGGTGTAGGTAGGAAGAGTCTGAAAAGACTCTGCACCCTCATCTAACTAATAATTTCAATTGCTTCACGACGAGGCGGCCATGA
AAATAGACCTTCAACATTTCTTTTGGAATCTTTGAATGGAAGCCGACGCAACTGTGAAAGATTAATTAATGCAATATCCAAGGATCGAACCTCGGCTCTCTGACCCTGACACCGCTACAATGGACGAAAGCCTGGGAGCTCGGCCGAGTCAGGGTCTTGTAAAAGAATCGCGGAAGGCCATAGCGCGCATTGAGTCGGAA
TTGGCAGGGAAGATCGAAGCCATCGCAGCCCTTCCCAATCCCCCCGGCATGGATATCTTAGTCTGCCAGGCTGCCAAGGCTAAGATGGAGGCCGGGGAGAAGAGAATTGCAGAAGCAAAAAAGGTTCCGCACAGATCCGTTATACAGCTTGAGGAGCTCTATCAAACGTACGACGCCATTGTTATCAAGTCCGCATCAGC
CATCGTGGACGACTATGAGAATATTCCTGCAATCTCAAGAGCGGAAACTGATCTTGATCGAAATGTGAAGCTAAATCCTGCAATCTCAAGAGCGGAAACTGATCTTGATCGAAATATGAAGCTAAATCCTGAACTGTTTCTGCAAGAACTGAAAGGGCTTATACTCTTTAACGTGCCAGACTCCTCACAGAAGCTCACGG
CCATCGCTAAACTCGACCTTTCTAATAACAACATCAAGGTAGATGCAGTATTACATATACTTTAGTGCAGGAAAGAAAATCTTTTGTGATTTTGCTAATAACTTATGAATTTCTTGTAGTGTCTGCCCGAACACTATAGTGCAGGAAATAATATAAATCTTTTGTGATTTTGCTAATAACTTGATGAATTTCATGTAGTG
TCTGCCTGAATCCGTGACAAGTCTGGTGAATTTGAGAATTCTCGACGTTCATTCGAATCAGCTCACATCTTTACCCAAATCCATGGGACGGCTTCCTAACCTCAAAATCCTCAATATTTCTGGAAATCTCTTCACCGATTTGCCACAAAGCGTTCAAAATTGCGGTTCGCTGGAAGAGTTGATTGCGGATTTCAACAAGC
TACAGCGGCTGCCTGAATCTCTGGGATTCAAACTCCTTAACCTGCAAAAGCTTTCCGTTCATTCCAACGAACTTTGTGATCTTCCTCTCTCCATCTGCCACATGATGTGTCTGCGTGTTCTGGACGTCCATATGAACAAACTTAGATATCTACCCGAAGATATTGAAAACCTTGTGAGCCTTCAAATTCTTGACGTTAGT
TGCAATTTCGACAACTTCAAAGCATTGCCGGATTCGATCTGTGGGCTGACTACTCTCGTGGCATTAGATGTGAGTTATAACCAGATTAAAACCCTACCCGATTTCATTGGAGGTCTCATGGAGCTGAAACGCCTAAATCTGGAGGGGAATCCGCTAATTCTACCACCGCCACAGATCGTTAGAGAAGGCCTCGAAGCGGT
TATGAATTACATGAGAAATCGAGCCACACAATGTCTCCTGCCGCCTAGTTTGTCATGCCTGTTAACCAAACAGCCACGTAAGTTGGTAGCTTGCGCAGGTGGAAACCGTGGGAGTAGAAACTTCAGTAGATACTTCCAAAAGCGATGACGTGGAACGAATGCAGATCTGAAGAGACGGCTTCTCGGCACCACCAGTTGTT
GCACTTCTAGTGGCTACCATCAACACCGAGATGCACTCCCCGCCATAAGAAGAACTAATGCCAACATTTTGCCAGCCTTATGCTTCTCAATTTCTCAAGCCTGCTACCATATAAAATGTTGAATTATTCTTTAAAGTTTGATCCATGAATGCACCTAGGAATTTAACTATTTATCCTCTCCAAATATATTATGTTTCTGT
GTTATAAAGTTTTGAAGTCGCAAGTTTCTGAATGATTAAATGAATAAAAAGGCTCTGTATTTTAGAAAAACGTGTTAAGTTTTCAGTTGTTCTTACATCACATTTTTCTGTATTATTTTTTACAAATTATATTGTATTATTTTTTACAAATTAT

>TRINITY_DN43864_c1_g4_i6 from sample A7 / Example unspliced isoform:

CAATAATAGCAACAGAGGAAAAAATAACGTAACTGCAACTTCTCTCTTATATACTCGTCGTTATTAATGGATGGAATTTTTCTACATGATGTTGCAACATCGATGCTGATCGGCCATTTCTGTTTTATACCCTTGTTGTTATGAATGGATGGAATAATTCCAAGGATAAACTGCAGCTCAGCCTTCAATTTCAAGAACAC
GTATCCTTTCTCTGACCAGATGATGTTGCATCATCGATGATGGTCGGCTTCTTGCCGTTTATGGTTCTGGACTTGGCACATTGCGTCTATTAATTCTCCGATTAATTTAGTATGGAAGGTGTAGGTAGGAAGAGTCTGAAAAGACTCTGCACCCTCATCTAACTAATAATTTCAATTGCTTCACGACGAGGCGGCCATGA
AAATAGACCTTCAACATTTCTTTTGGAATCTTTGAATGGAAGCCGACGCAACTGTGAAAGATTAATTAATGCAATATCCAAGGATCGAACCTCGGCTCTCTGACCCTGACACCGCTACAATGGACGAAAGCCTGGGAGCTCGGCCGAGTCAGGGTCTTGTAAAAGAATCGCGGAAGGCCATAGCGCGCATTGAGTCGGAA
TTGGCAGGGAAGATCGAAGCCATCGCAGCCCTTCCCAATCCCCCCGGCATGGATATCTTAGTCTGCCAGGCTGCCAAGGCTAAGATGGAGGCCGGGGAGAAGAGAATTGCAGAAGCAAAAAAGGTTCCGCACAGATCCGTTATACAGCTTGAGGAGCTCTATCAAACGTACGACGCCATTGTTATCAAGTCCGCATCAGC
CATCGTGGACGACTATGAGAATATTCCTGCAATCTCAAGAGCGGAAACTGATCTTGATCGAAATGTGAAGCTAAATCCTGCAATCTCAAGAGCGGAAACTGATCTTGATCGAAATATGAAGCTAAATCCTGAACTGTTTCTGCAAGAACTGAAAGGGCTTATACTCTTTAACGTGCCAGACTCCTCACAGAAGCTCACGG
CCATCGCTAAACTCGACCTTTCTAATAACAACATCAAGTGTCTGCCTGAATCCGTGACAAGTCTGGTGAATTTGAGAATTCTCGACGTTCATTCGAATCAGCTCACATCTTTACCCAAATCCATGGGACGGCTTCCTAACCTCAAAATCCTCAATATTTCTGGAAATCTCTTCACCGATTTGCCACAAAGCGTTCAAAAT
TGCGGgtaaaactctatttcgaatcattaatttcttccgcaaacatttaattctgcacggtttgaactttgctcaattgtatgattttgccgctaatagaaatttatcagTTCGCTGGAAGAGTTGATTGCGGATTTCAACAAGCTACAGCGGCTGCCTGAATCTCTGGGATTCAAACTCCTTAACCTGCAAAAGCTTTC
CGTTCATTCCAACGAACTTTGTGATCTTCCTCTCTCCATCTGCCACATGATGTGTCTGCGTGTTCTGGACGTCCATATGAACAAACTTAGATATCTACCCGAAGATATTGAAAACCTTGTGAGCCTTCAAATTCTTGACGTTAGTTGCAATTTCGACAACTTCAAAGCATTGCCGGATTCGATCTGCGGGCTGACTACAC
TCGTGGCATTAGATGTGAGTTATAACCAGATTAAAACCCTACCCGATTTCATTGGAGGTCTCATGGAGCTGAAACGCCTAAATCTGGAGGGGAATCCGCTAATTCTACCACCGCCACAGATCGTTAGAGAAGGCCTCGAAGCGGTTATGAATTACATGAGAAATCGAGCCACACAATGTCTCCTGCCGCCTAGTTTGTCA
TGCCTGTTAACCAAACAGCCACGTAAGTTGGTAGCTTGCGCAGGTGGAAACCGTGGGAGTAGAAACTTCAGTAGATACTTCCAAAAGCGATGACGTGGAACGAATGCAGATCTGAAGAGACGGCTTCTCGGCACCACCAGTTGTTGCACTTCTAGTGGCTACCATCAACACCGAGATGCACTCCCCGCCATAAGAAGAAC
TAATGTCAACATTTTAGCAGCCTTATGCTTCTCAATTTCTCAAGCCTGCTACCATATAAAATGTTGAATTATTCTTTAAAGTTTGATCCATGAATGCACCTAGGAATTTAACTATTTATCCTCTCCAAATATATTATGTTTCTGTGTTATAAAGTTTTGAAGTCGCAAGTTTCTGAATGATTAAATGAATAAAAAGGCTC
TGTATTTTAGAAAAACGTGTTAAGTTTTCAGTTGTTCTTACATCACATTTTTCTGTATTATTTTTTACAAATTATATTGTATTATTTTTTACAAATTAT