sp|Q6PS57|CKS1_ORYSJ ----> Cyclin-dependent kinases regulatory subunit 1

Intron 1 GTATGATACA ... TGTTTAACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	65.85	7	18	9
A2	84.62	1	9	2
A3	62.96	5	7	10
A4	88.89	1	9	7
A5	81.82	1	5	4
A6	46.67	4	5	2
A7	62.5	3	5	5
A8	50	3	5	1
A9	55.56	2	2	3
A10	50	4	4	4

>TRINITY_DN44176_c4_g2_i6 from sample A6 / Example spliced isoform

AGCAATATTCATACACATATTGATATTCAGACGGAGGAGATTCTGCGTAGTCCTATCTTCTTGTTTTGATCACGGACGAAGATCCTCAAATTCTTGATTAGTGCACATTGATGATCTTCGACGGTAACCACAATGCTCTGATTGAAGAGTGCGTCATCGTGGCGGTGATCAGATCGATTCCTCGGCTGCTTGTGTTAAAT
ATATCAGTGGAGATTAATTGCCCTCTTCATGATCAGAGTGATTTCTCCACAATGCTCAGAGCCATTCCTCCACAATGCTCTGCTGCGTGGAGACATTGAACAACTGCCAACTTCTCGGCCCCTGCCGTGACTCTGGTGATAAATGGCTATGCGTTTTTTGCCTGTACCATCTTGGTTGTTATACTAGATCTATTTTCTCC
AGCGTTGCAAACCACGGTTCTAGAGGATTCGAACCCGAGACGAGGACAAGGACGAGGTTCTTGAGGGTTCGAACGCGGGACGAGGACGAGGACGAGGTTCCCAAATGCTGCCAGGCTTTTCACATCGACTGTATTGAGATCTCATGTCAATTGCCCTCGCTGCCGCTCCAATTATAAATATCGTTTCCGTTCTTCAATGT
AGCAATAACTCCGATAGCATATCCTACCATTCTCGACGATTTTCAATCACGTATCTGAAAATTTGATGTGTGAAAGATTTTTTCCCTTGCAGTTCTTTTTGTTTTACAATTTTTTTCTTACAGTTCTTGTATGTTTACTTAGTTTCTTTCATCAAAACTGCAATCCATTCTTAACGCTGAGAAACCCTAGGGTTTGCTTT
CTTGTTTACTTTCTTGTTTAACAGGCCTGGAAAATGTCTCAAATATGGTATTCTGAAAAATACTTCGATGATATCTATGAGTATATGGGACAGTAAAATGTCTCTAATACAATATTCTGAAAAATACTTTGATGATATCTATGAGTACAGGTATGATACAGGTATGTGACGCTCGGTCGTGATGTTGCGCGGCTTTTCTC
CAGGACCCCATATTGAATGAGGTTGGATGATGGTACATATGAGGAGGAAGATGGGATTCTCACTCGATAACAAATCAACACTTTTCATGTTAAATGATAAACAAGTAAAGTGGCTATGAGGGAAGATACAAGTGCAAAATAAAAACTGAAAGAAAGCATGATGTGGTGCTGGATTGAAGATTGATGCAAAGCAGTTCAAG
ACCAGAGATTCTAAAGTTCTCAAGCGCTCTCTCCCTTAGAGCACTTGTCCCAGCTAGAGCTAGATGTTATCACTATTGAATGAGTTGATGATGATTACCGGCAGTGACGCCCCAGAGCCGGATGTTCCAGACTCGACAGGGGGG

>TRINITY_DN44176_c4_g2_i7 from sample A6 / Example unspliced isoform:

AGCAATATTCATACACATATTGATATTCAGACGGAGGAGATTCTGCGTAGTCCTATCTTCTTGTTTTGATCACGGACGAAGATCCTCAAATTCTTGATTAGTGCACATTGATGATCTTCGACGGTAACCACAATGCTCTGATTGAAGAGTGCGTCATCGTGGCGGTGATCAGATCGATTCCTCGGCTGCTTGTGTTAAAT
ATATCAGTGGAGATTAATTGCCCTCTTCATGATCAGAGTGATTTCTCCACAATGCTCAGAGCCATTCCTCCACAATGCTCTGCTGCGTGGAGACATTGAACAACTGCCAACTTCTCGGCCCCTGCCGTGACTCTGGTGATAAATGGCTATGCGTTTTTTGCCTGTACCATCTTGGTTGTTATACTAGATCTATTTTCTCC
AGCGTTGCAAACCACGGTTCTAGAGGATTCGAACCCGAGACGAGGACAAGGACGAGGTTCTTGAGGGTTCGAACGCGGGACGAGGACGAGGACGAGGTTCCCAAATGCTGCCAGGCTTTTCACATCGACTGTATTGAGATCTCATGTCAATTGCCCTCGCTGCCGCTCCAATTATAAATATCGTTTCCGTTCTTCAATGT
AGCAATAACTCCGATAGCATATCCTACCATTCTCGACGATTTTCAATCACGTATCTGAAAATTTGATGTGTGAAAGATTTTTTCCCTTGCAGTTCTTTTTGTTTTACAATTTTTTTCTTACAGTTCTTGTATGTTTACTTAGTTTCTTTCATCAAAACTGCAATCCATTCTTAACGCTGAGAAACCCTAGGGTTTGCTTT
CTTGTTTACTTTCTTGTTTAACAGGCCTGGAAAATGTCTCAAATATGGTATTCTGAAAAATACTTCGATGATATCTATGAGTATATGGgtatgatacatttcttgtttgaaggcatttgattatatagtctttgaatttctcctttcaaggtataaaaattagtgtataaagacaataataatttcctttcggattcagc
tttaaaattagggtttgaaaaccgaagtcatttttctcttacagatgtgagaaaccgtagggttttcttcgttgtttaacagGACAGTAAAATGTCTCTAATACAATATTCTGAAAAATACTTTGATGATATCTATGAGTATAATATTCTGAAAAATACTTTGATGATATCTATGAGTATAGGTATGATACATTTCTTGT
TTCCAGGCATTTGAATTATTGATTCTTTGAAATTCTCCATTCACGGTTTAAAAATCAGGGCTTAATAATAAAATGCTTTGAATAGTCGTCGTGTTTGTTTATATGTTCAGGTATGTGACGCTCGGTCGTGATGTTGCGCGGCTTTTCTCCAGGACCCCATATTGAATGAGGTATGTGACGCTCGGTCGTGATATTGAATG
AGTTGATGATGATTACCGGCAGTGACGCCCCAGAGCCGGATGTTCCAGACTCGACAGGGGGG

Intron 2 GTATGTGACG ... ATTGAATGAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	45	11	5	13
A2	33.33	7	2	5
A3	58.82	7	7	13
A4	47.37	5	3	6
A5	33.33	2	1	1
A6	33.33	3	2	1
A7	36.36	7	2	6
A8	50	3	3	3
A9	33.33	1	1	0
A10	77.78	1	3	4

>TRINITY_DN44243_c0_g1_i1 from sample A9 / Example spliced isoform

AGACATTGAACAACTGCCAACTTCTCGGCCCCTGCCGTGACTCTGGGTTGTCATACTACATCTATTTTCTCCAGCGTTGCAAACCACGGTTCTAGAGGATTCGAACCCGAGACGAGGACAAGGACGAGGTTCTTGAGGGTTCGAACGCGGGACGAGGACGAGGACGAGGTTCCCAAATGCTGCCAGGCTTTTCACATCGA
CTGTATTGAGATCTCATGTCAATTGCCCTCGCTGCCGCTCCAATTATAAATATCGTTTCCGTTCTTCAATGTAGCAATAACTCCGATAGCATATCCTACCATTCTCGACGATTTTCAATCACGTATCTGAAAATTTGATGCCTGGAAAATGTCTCAAATATGGTATTCTGAAAAATACTTCGATGATATCTATGAGTATA
TGGGTATGATACATTTCTTGTTTGAAGGCATTTGATTATATAGTCTTTGAATTTCTCCTTTCAAGGTATAAAAATTAGTGTATAAAGACAATAATAATTTCCTTTCGGATTCAGCTTTAAAATTAGGGTTTAAAGACCGAAGTCATTTTTCTCTTACAGATGTGAGAAACCGTAGGGTTTTCTTCGTTGTTTAACAGGAC
AGTAAAATGTCTCTAATACAATATTCTGAAAAATACTTTGATGATATCTATGAGTACAGGTATGATACATTTCTTGTTTCCAGGTATTTGAATTATTGATTCTTTGAAATTCTCCATTCACGGTTTAAAAATCAGGGCTTAATAATAAAATGCTTTGAATAGTCGTCGTGTTTGTTTATATGTTCAGGTATGTGACGCTC
GGTCGTGATATTGAATGAGGTAAGCCATGCTTACTTATTGGTTTGTTTTGCTGTATAAATTCTTTCTTAACTGAGATGATCTCTGCTGTTCTTCACCTAACATAGATAACATAGATCTGCTAGAGGAGATATGTTCTGTTACCAATATTACTCCTGAAATCATATCACTAGGCGAAACTTTAGACCAACTTTGGTCGAAG
TGATTACAATTATTTGAGTTTCTTTCACTGTTTGTGCGGGTCGTATAGAGGGAGGGTCATCCTGATTAGTGCATACAGCCAGCGTCATTTTGTTTTAAAGGCATTTTCATGGCTTAAATAGGTACATTCATACCCTTGAAGCGTGCGTTTAATGAAGTATACGACCTTGTGTTCCAAGTTTGTCAATCCTCGTAAGCCAT
GCTTACTTATTGGTTTGTTTTGCTGTATAAATTCTTTCTTAACTGAGATGATCTCTGCTGTTCTTCACCTAACATAGATAACATAGATCTGCTAGAGGAGATATGTTCTGTTACCAATATTACTCCTGAAATCATATCACTAGGCGAAACTTTAGACCAACTTTGGTCGAAGTGATTACAATTATTTGAGTTTCTTTCAC
TGTTTGTGCGGGTCGTATAGAGGGAGGGTCATCCTGATTAGTGCATACAGCCAGCGTCATTTTGTTTTAAAGGCATTTTCATGGCTTAAATAGGTACATTCATACCCTTGAAGCGTGCGTTTAATGAAGTATACGACCTTGTGTTCCAAGTTTGTCAATCCTC

>TRINITY_DN44243_c0_g1_i13 from sample A9 / Example unspliced isoform:

ATTCTCCATTCACGGTTTAAAAATCAGGGCTTAATAATAAAATGCTTTGAATAGTCGTCGTGTTTGTTTATATGTTCAGGTATGTGACGCTCGGTCGTGATGTTGCGCGGCTTTTCTCCAGGACCCCATATTGAATGAGATTGAATGAGGTAAGCCATGCTTACTTATTGGTTTGTTTTGCTGTATAAATTCTTTCTTAA
CTGAGATGATCTCTGCTGTTCTTCACCTAACATAGATAACATAGATCTGCTAGAGGAGATATGTTCTGTTACCAATATTACTCCTGAAATCATATCACTAGGCGAAACTTTAGACCAACTTTGGTCGAAGTGATTACAATTATTTGAGTTTCTTTCACTGTTTGTGCGGGTCGTATAGAGGGAGGGTCATCCTGATTAGT
GCATACAGCCAGCGTCATTTTGTTTTAAAGGCATTTTCATGGCTTAAATAGGTACATTCATACCCTTGAAGCGTGCGTTTAATGAAGTATACGACCTTGTGTTCCAAGTTTGTCAATCCTCgtatgtgacgctcggtcgtgatattgaatgagGTAAGCCATGCTTACTTATTGGTTTGTTTTGCTGTATAAATTCTTTC
TTAACTGAGATGATCTCTGCTGTTCTTCACCTAACATAGATAACATAGATCTGCTAGAGGAGATATGTTCTGTTACCAATATTACTCCTGAAATCATATCACTAGGCGAAACTTTAGACCAACTTTGGTCGAAGTGATTACAATTATTTGAGTTTCTTTCACTGTTTGTGCGGGTCGTATAGAGGGAGGGTCATCCTGAT
TAGTGCATACAGCCAGCGTCATTTTGTTTTAAAGGCATTTTCATGGCTTAAATAGGTACATTCATACCCTTGAAGCGTGCGTTTAATGAAGTATACGACCTTGTGTTCCAAGTTTGTCAATCCTC

Intron 3 GTATGATACA ... ATGAGTACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	88.28	2.4	18	1.4
A2	85.09	1.6	9	0.4
A3	65	4.6	7	5.4
A4	100	0	9	4
A5	75	1.8	5	2
A6	90	0.6	5	0.4
A7	47.27	5.6	5	3.8
A8	50	5	5	3.2
A9	33.33	4	2	4.2
A10	66.28	2.2	4	0.8

>TRINITY_DN44243_c0_g1_i17 from sample A9 / Example spliced isoform

AGACATTGAACAACTGCCAACTTCTCGGCCCCTGCCGTGACTCTGGGTTGTCATACTACATCTATTTTCTCCAGCGTTGCAAACCACGGTTCTAGAGGATTCGAACCCGAGACGAGGACAAGGACGAGGTTCTTGAGGGTTCGAACGCGGGACGAGGACGAGGACGAGGTTCCCAAATGCTGCCAGGCTTTTCACATCGA
CTGTATTGAGATCTCATGTCAATTGCCCTCGCTGCCGCTCCAATTATAAATATCGTTTCCGTTCTTCAATGTAGCAATAACTCCGATAGCATATCCTACCATTCTCGACGATTTTCAATCACGTATCTGAAAATTTGATGCCTGGAAAATGTCTCAAATATGGTATTCTGAAAAATACTTCGATGATATCTATGAGTATA
TGGGTATGTGACGCTCGGTCGTGATGTTGCGCGGCTTTTCTCCAGGACCCCATATTGAATGAGATTGAATGAGGTAAGCCATGCTTACTTATTGGTTTGTTTTGCTGTATAAATTCTTTCTTAACTGAGATGATCTCTGCTGTTCTTCACCTAACATAGATAACATAGATCTGCTAGAGGAGATATGTTCTGTTACCAAT
ATTACTCCTGAAATCATATCACTAGGCGAAACTTTAGACCAACTTTGGTCGAAGTGATTACAATTATTTGAGTTTCTTTCACTGTTTGTGCGGGTCGTATAGAGGGAGGGTCATCCTGATTAGTGCATACAGCCAGCGTCATTTTGTTTTAAAGGCATTTTCATGGCTTAAATAGGTACATTCATACCCTTGAAGCGTGC
GTTTAATGAAGTATACGACCTTGTGTTCCAAGTTTGTCAATCCTCGTATGTGACGCTCGGTCGTGATATTGAATGAGTTGCGGCTATGGATGCCAATGCCGAAATATTGATTGAGCTTGCC

>TRINITY_DN44243_c0_g1_i4 from sample A9 / Example unspliced isoform:

GCGTTTTATATATATGTATATATTCATGTGTTTTCTTTGCCTTCTAAATGCTAAGAACTGTCAGGTTCTGTCAATGCAGGGTTGTCATACTACATCTATTTTCTCCAGCGTTGCAAACCACGGTTCTAGAGGATTCGAACCCGAGACGAGGACAAGGACGAGGTTCTTGAGGGTTCGAACGCGGGACGAGGACGAGGACG
AGGTTCCCAAATGCTGCCAGGCTTTTCACATCGACTGTATTGAGATCTCATGTCAATTGCCCTCGCTGCCGCTCCAATTATAAATATCGTTTCCGTTCTTCAATGTAGCAATAACTCCGATAGCATATCCTACCATTCTCGACGATTTTCAATCACGTATCTGAAAATTTGATGTGTGAAAGATTTTTTCCCTTGCAGTT
CTTTTTGTTTTACAATTTTTTTCTTACAGTTCTTGTATGTTTACTTAGTTTCTTTCATCAAAACTGCAATCCATTCTTAACGCTGAGAAACCCTAGGGTTTGCTTTCTTGTTTACTTTCTTGTTTAACAGGCCTGGAAAATGTCTCAAATATGGTATTCTGAAAAATACTTCGATGATATCTATGAGTATATGGgtatga
tacatttcttgtttgaaggcatttgattatatagtctttgaatttctcctttcaaggtataaaaattagtgtataaagacaataataatttcctttcggattcagctttaaaattagggtttaaagaccgaagtcatttttctcttacagatgtgagaaaccgtagggttttcttcgttgtttaacaggacagtaaaatg
tctctaatacaatattctgaaaaatactttgatgatatctatgagtacagGTATGTGACGCTCGGTCGTGATGTTGCGCGGCTTTTCTCCAGGACCCCATATTGAATGAGGTTGGATGATGGTACATATAACGAGGAAGATGGGACTCTCACTCAATAACAAATCAACACTTTTCATGTTAAATGATAAACAGGTAAAGG
TATGTATATCCATTGATTGATACCAATAATCAAGGAAATACCAATAATCAAGGAAAGGAAAGAAATATGTAGATCATATTAGATTTTGATAGCTTTCATCTTAGAAATGTACTAATGTATGCACAATAAAAAAG

Intron 4 GTGTGAAAGA ... TGTTTAACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	96.15	1	21	28.9
A2	58.12	7	8	11.5
A3	58.38	9	15	10.3
A4	86.96	3	18	22
A5	70.63	2	6	3.6
A6	60	5	8	7
A7	64.85	5.9	11	10
A8	51.64	13.2	16	12
A9	46.34	16	21	6.6
A10	54.05	17	26	14

>TRINITY_DN44243_c0_g1_i15 from sample A9 / Example spliced isoform

AGACATTGAACAACTGCCAACTTCTCGGCCCCTGCCGTGACTCTGGGTTGTCATACTACATCTATTTTCTCCAGCGTTGCAAACCACGGTTCTAGAGGATTCGAACCCGAGACGAGGACAAGGACGAGGTTCTTGAGGGTTCGAACGCGGGACGAGGACGAGGACGAGGTTCCCAAATGCTGCCAGGCTTTTCACATCGA
CTGTATTGAGATCTCATGTCAATTGCCCTCGCTGCCGCTCCAATTATAAATATCGTTTCCGTTCTTCAATGTAGCAATAACTCCGATAGCATATCCTACCATTCTCGACGATTTTCAATCACGTATCTGAAAATTTGATGCCTGGAAAATGTCTCAAATATGGTATTCTGAAAAATACTTCGATGATATCTATGAGTATA
TGGATGTGAGAAACCGTAGGGTTTTCTTCGTTGTTTAACAGGACAGTAAAATGTCTCTAATACAATATTCTGAAAAATACTTTGATGATATCTATGAGTACAGGTATGTGACGCTCGGTCGTGATGTTGCGCGGCTTTTCTCCAGGACCCCATATTGAATGAGGTTGGATGATGGTACATATGAGGAGGAAGATGGGATT
CTCACTGGATAACAAATCAACACTTTTCATGTTAAATGATAAACAAGTAAAGTGGCTATGAGGGAAGATACAAGTGCAAAATAAAAACTGAAAGAAAGCATGATGTGGTGCTGGATTGAAGATTGATGCAAAGCAGTTCAAGACCAGAGATTCTAAAGTTCTCAAGCGCTCTCTCCCTTAGAGCACTTGTCCCAGCTAGA
GCTAGATGTTATCACACCATAGTAGCAGGGCTATTATTCTTGCTTAGCAGATATCTACACATTTCAGAGGCTG

>TRINITY_DN44243_c0_g1_i4 from sample A9 / Example unspliced isoform:

GCGTTTTATATATATGTATATATTCATGTGTTTTCTTTGCCTTCTAAATGCTAAGAACTGTCAGGTTCTGTCAATGCAGGGTTGTCATACTACATCTATTTTCTCCAGCGTTGCAAACCACGGTTCTAGAGGATTCGAACCCGAGACGAGGACAAGGACGAGGTTCTTGAGGGTTCGAACGCGGGACGAGGACGAGGACG
AGGTTCCCAAATGCTGCCAGGCTTTTCACATCGACTGTATTGAGATCTCATGTCAATTGCCCTCGCTGCCGCTCCAATTATAAATATCGTTTCCGTTCTTCAATGTAGCAATAACTCCGATAGCATATCCTACCATTCTCGACGATTTTCAATCACGTATCTGAAAATTTGATgtgtgaaagattttttcccttgcagtt
ctttttgttttacaatttttttcttacagttcttgtatgtttacttagtttctttcatcaaaactgcaatccattcttaacgctgagaaaccctagggtttgctttcttgtttactttcttgtttaacagGCCTGGAAAATGTCTCAAATATGGTATTCTGAAAAATACTTCGATGATATCTATGAGTATATGGGTATGA
TACATTTCTTGTTTGAAGGCATTTGATTATATAGTCTTTGAATTTCTCCTTTCAAGGTATAAAAATTAGTGTATAAAGACAATAATAATTTCCTTTCGGATTCAGCTTTAAAATTAGGGTTTAAAGACCGAAGTCATTTTTCTCTTACAGATGTGAGAAACCGTAGGGTTTTCTTCGTTGTTTAACAGGACAGTAAAATG
TCTCTAATACAATATTCTGAAAAATACTTTGATGATATCTATGAGTACAGGTATGTGACGCTCGGTCGTGATGTTGCGCGGCTTTTCTCCAGGACCCCATATTGAATGAGGTTGGATGATGGTACATATAACGAGGAAGATGGGACTCTCACTCAATAACAAATCAACACTTTTCATGTTAAATGATAAACAGGTAAAGG
TATGTATATCCATTGATTGATACCAATAATCAAGGAAATACCAATAATCAAGGAAAGGAAAGAAATATGTAGATCATATTAGATTTTGATAGCTTTCATCTTAGAAATGTACTAATGTATGCACAATAAAAAAG

Intron 5 GTTGCGCGGC ... ATTGAATGAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	47.07	13	7.8	16
A2	71.03	3.5	8	8.5
A3	43	13	7.8	12
A4	34.47	8.5	7	6
A5	76.78	1.5	4.8	3.5
A6	79.17	0.5	1.8	1.5
A7	61.31	5	6	10
A8	40.92	4	2.3	3.5
A9	75	1	2.3	2
A10	53.75	2	2.8	2.5

>TRINITY_DN44243_c0_g1_i13 from sample A9 / Example spliced isoform

ATTCTCCATTCACGGTTTAAAAATCAGGGCTTAATAATAAAATGCTTTGAATAGTCGTCGTGTTTGTTTATATGTTCAGGTATGTGACGCTCGGTCGTGATGTTGCGCGGCTTTTCTCCAGGACCCCATATTGAATGAGATTGAATGAGGTAAGCCATGCTTACTTATTGGTTTGTTTTGCTGTATAAATTCTTTCTTAA
CTGAGATGATCTCTGCTGTTCTTCACCTAACATAGATAACATAGATCTGCTAGAGGAGATATGTTCTGTTACCAATATTACTCCTGAAATCATATCACTAGGCGAAACTTTAGACCAACTTTGGTCGAAGTGATTACAATTATTTGAGTTTCTTTCACTGTTTGTGCGGGTCGTATAGAGGGAGGGTCATCCTGATTAGT
GCATACAGCCAGCGTCATTTTGTTTTAAAGGCATTTTCATGGCTTAAATAGGTACATTCATACCCTTGAAGCGTGCGTTTAATGAAGTATACGACCTTGTGTTCCAAGTTTGTCAATCCTCGTATGTGACGCTCGGTCGTGATATTGAATGAGGTAAGCCATGCTTACTTATTGGTTTGTTTTGCTGTATAAATTCTTTC
TTAACTGAGATGATCTCTGCTGTTCTTCACCTAACATAGATAACATAGATCTGCTAGAGGAGATATGTTCTGTTACCAATATTACTCCTGAAATCATATCACTAGGCGAAACTTTAGACCAACTTTGGTCGAAGTGATTACAATTATTTGAGTTTCTTTCACTGTTTGTGCGGGTCGTATAGAGGGAGGGTCATCCTGAT
TAGTGCATACAGCCAGCGTCATTTTGTTTTAAAGGCATTTTCATGGCTTAAATAGGTACATTCATACCCTTGAAGCGTGCGTTTAATGAAGTATACGACCTTGTGTTCCAAGTTTGTCAATCCTC

>TRINITY_DN44243_c0_g1_i17 from sample A9 / Example unspliced isoform:

AGACATTGAACAACTGCCAACTTCTCGGCCCCTGCCGTGACTCTGGGTTGTCATACTACATCTATTTTCTCCAGCGTTGCAAACCACGGTTCTAGAGGATTCGAACCCGAGACGAGGACAAGGACGAGGTTCTTGAGGGTTCGAACGCGGGACGAGGACGAGGACGAGGTTCCCAAATGCTGCCAGGCTTTTCACATCGA
CTGTATTGAGATCTCATGTCAATTGCCCTCGCTGCCGCTCCAATTATAAATATCGTTTCCGTTCTTCAATGTAGCAATAACTCCGATAGCATATCCTACCATTCTCGACGATTTTCAATCACGTATCTGAAAATTTGATGCCTGGAAAATGTCTCAAATATGGTATTCTGAAAAATACTTCGATGATATCTATGAGTATA
TGGGTATGTGACGCTCGGTCGTGATgttgcgcggcttttctccaggaccccatattgaatgagATTGAATGAGGTAAGCCATGCTTACTTATTGGTTTGTTTTGCTGTATAAATTCTTTCTTAACTGAGATGATCTCTGCTGTTCTTCACCTAACATAGATAACATAGATCTGCTAGAGGAGATATGTTCTGTTACCAAT
ATTACTCCTGAAATCATATCACTAGGCGAAACTTTAGACCAACTTTGGTCGAAGTGATTACAATTATTTGAGTTTCTTTCACTGTTTGTGCGGGTCGTATAGAGGGAGGGTCATCCTGATTAGTGCATACAGCCAGCGTCATTTTGTTTTAAAGGCATTTTCATGGCTTAAATAGGTACATTCATACCCTTGAAGCGTGC
GTTTAATGAAGTATACGACCTTGTGTTCCAAGTTTGTCAATCCTCGTATGTGACGCTCGGTCGTGATATTGAATGAGTTGCGGCTATGGATGCCAATGCCGAAATATTGATTGAGCTTGCC

Intron 6 GTATGATACA ... TCTCTTACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	88.86	2	15.3	17
A2	100	0	8.3	6
A3	89.5	1	5	12.7
A4	100	0	8.7	16
A5	100	0	4.7	5.7
A6	100	0	4.3	2
A7	100	0	4.7	5
A8	49.58	4	4.7	3.3
A9	66.67	2	2	6
A10	100	0	3	7

>TRINITY_DN44243_c0_g1_i15 from sample A9 / Example spliced isoform

AGACATTGAACAACTGCCAACTTCTCGGCCCCTGCCGTGACTCTGGGTTGTCATACTACATCTATTTTCTCCAGCGTTGCAAACCACGGTTCTAGAGGATTCGAACCCGAGACGAGGACAAGGACGAGGTTCTTGAGGGTTCGAACGCGGGACGAGGACGAGGACGAGGTTCCCAAATGCTGCCAGGCTTTTCACATCGA
CTGTATTGAGATCTCATGTCAATTGCCCTCGCTGCCGCTCCAATTATAAATATCGTTTCCGTTCTTCAATGTAGCAATAACTCCGATAGCATATCCTACCATTCTCGACGATTTTCAATCACGTATCTGAAAATTTGATGCCTGGAAAATGTCTCAAATATGGTATTCTGAAAAATACTTCGATGATATCTATGAGTATA
TGGATGTGAGAAACCGTAGGGTTTTCTTCGTTGTTTAACAGGACAGTAAAATGTCTCTAATACAATATTCTGAAAAATACTTTGATGATATCTATGAGTACAGGTATGTGACGCTCGGTCGTGATGTTGCGCGGCTTTTCTCCAGGACCCCATATTGAATGAGGTTGGATGATGGTACATATGAGGAGGAAGATGGGATT
CTCACTGGATAACAAATCAACACTTTTCATGTTAAATGATAAACAAGTAAAGTGGCTATGAGGGAAGATACAAGTGCAAAATAAAAACTGAAAGAAAGCATGATGTGGTGCTGGATTGAAGATTGATGCAAAGCAGTTCAAGACCAGAGATTCTAAAGTTCTCAAGCGCTCTCTCCCTTAGAGCACTTGTCCCAGCTAGA
GCTAGATGTTATCACACCATAGTAGCAGGGCTATTATTCTTGCTTAGCAGATATCTACACATTTCAGAGGCTG

>TRINITY_DN44243_c0_g1_i11 from sample A9 / Example unspliced isoform:

GCGTTTTATATATATGTATATATTCATGTGTTTTCTTTGCCTTCTAAATGCTAAGAACTGTCAGGTTCTGTCAATGCAGGGTTGTCATACTACATCTATTTTCTCCAGCGTTGCAAACCACGGTTCTAGAGGATTCGAACCCGAGACGAGGACAAGGACGAGGTTCTTGAGGGTTCGAACGCGGGACGAGGACGAGGACG
AGGTTCCCAAATGCTGCCAGGCTTTTCACATCGACTGTATTGAGATCTCATGTCAATTGCCCTCGCTGCCGCTCCAATTATAAATATCGTTTCCGTTCTTCAATGTAGCAATAACTCCGATAGCATATCCTACCATTCTCGACGATTTTCAATCACGTATCTGAAAATTTGATGTGTGAAAGATTTTTTCCCTTGCAGTT
CTTTTTGTTTTACAATTTTTTTCTTACAGTTCTTGTATGTTTACTTAGTTTCTTTCATCAAAACTGCAATCCATTCTTAACGCTGAGAAACCCTAGGGTTTGCTTTCTTGTTTACTTTCTTGTTTAACAGGCCTGGAAAATGTCTCAAATATGGTATTCTGAAAAATACTTCGATGATATCTATGAGTATATGGgtatga
tacatttcttgtttgaaggcatttgattatatagtctttgaatttctcctttcaaggtataaaaattagtgtataaagacaataataatttcctttcggattcagctttaaaattagggtttaaagaccgaagtcatttttctcttacagATGTGAGAAACCGTAGGGTTTTCTTCGTTGTTTAACAGGACAGTAAAATG
TCTCTAATACAATATTCTGAAAAATACTTTGATGATATCTATGAGTACAGGTATGTGACGCTCGGTCGGTCGTGATGTTGCGCGGCTTTTCTCCAGGACCCCATATTGAATGAGCTTCTTTGTGGTCAGCTTCTTAACTTAAGGTTGGATGATGGTACATATGAGGAGGAAGATGGGATTCTCACT