sp|Q9C8Y2|CAR2_ARATH ----> Protein C2-DOMAIN ABA-RELATED 2

Intron 1 GCAAGTTGGC ... AAAATTTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	81.82	28	3	126
A2	84.87	23	0	129
A3	79.67	50	4	196
A4	80.73	21	0	88
A5	84.21	9	1	48
A6	82.3	111	3	516
A7	82.32	148	9	689
A8	84.95	76	2	429
A9	82.41	127	17	595
A10	81.86	127	2	573

>TRINITY_DN37007_c0_g1_i3 from sample A7 / Example spliced isoform

ATGTGCCAAGCCGGGCTTTGCGGATGAGCTTTCCCTGTCCCTGTTCCTGTTCCCTCTCCTTTTTGGAAATGAAAATGTAAAAGTGATCGTGGTATTTGGAAAATTGGAGGTAGGCTGTCCCGTCAAATCGGAGAATAAAAAGAACGTCAATTCATTGTCACAAGGCTTCATTGATGATTGGGAGAGAGTGAGAGATGGAG
ACGATATTGGGTCTTCTGAAAGTCCGCGTGGTAAAGGGGAGTAACTTGGCTGTTCGGGACCGGACGAGCAGTGATCCCTATGTTGTAGTCAAGCTGGGTAATCAAACAGTTAAGACACGAGTAATTAAGAGGGACCTTAATCCAGTTTGGGATGAAGAATTGACGCTATCTATACCAAATCCAACTCCACCACTTAAACT
GCAAGTATTTGACAAAGATACATTAAGCAGAGATGACAAGATGGGAGATGCCGTAATCGATCTGCAACCTCTAGTCATGGCTGTAAGCATGAGAAATGCGTTGCCATTAACATTGACAAGCAAATCTGAGACTGAATTGTACAGATTGGTGGCATGCAAAGACAACTATCTTGTGAAAGACAGTTGCATTCGGCATGTTG
ATGGAAACACAGTTCAGGAAATATACTTGAGGCTTCAGAACGTTGAGTGTGGTGAACTGGAATTGCAGCTCAAGTGGGTTGATCTTCCCCGGGATTAGGAATAGGAATATCAGATCAGCTAGCCAGGCCGACAGTGTCTCAGCATGTACACTTCATATTACCTTTGCAAGGCTTTGGCTTCCATATCTTTTCAAGGGTCT
AAACTTAGTGCTAAATAAAATATGATCATTGGTTCAGGCTTCTGCTTTGTTTTAGTCAAAGGATAAGAAGAGGCATAGACTGATTCAGACATCATAAATACATAAATATATTTGCATTAGCACCATGTACATGAATTTATCGTACTGCGTTGCACAGGGTATATCTAAACAACCTTCATAAATGTTGAGTATAACTAAGT
TTTCTAATTTTTTCTCTGAAATGCTATCATTACTTTCTCTTAGAGCAGGAGATATGTAAACAACCTTCATAGTTGTGTAGCATAATTTAGTTTTAGTGCATCTTCATTGTAGTTACACACATTCATATGACTCATGGTAAAGTTGGCATCCAAGAGACAGAAACTCCTTTCTAGATATTATCTTTATAATAACAACTTAT
TGCACCAAGAATAGTGAGGTTTTCTACAGCCCAATATGATATAGATGATGTCATGCATATAATACTTATATGGTGGCTTAAAAAACACAATACTTATAATAAAACATCTCGAC

>TRINITY_DN37007_c0_g1_i1 from sample A7 / Example unspliced isoform:

ATGTGCCAAGCCGGGCTTTGCGGATGAGCTTTCCCTGTCCCTGTTCCTGTTCCCTCTCCTTTTTGGAAATGAAAATGTAAAAGTGATCGTGGTATTTGGAAAATTGGAGGTAGGCTGTCCCGTCAAATCGGAGAATAAAAAGAACGTCAATTCATTGTCACAAGGCTTCATTGATGATTGGGAGgcaagttggcaaccga
actacaacaaacccgtggtgtcaactgtaaccaacttttcacttattcaacggcagagtaattttgttaagactaatttattaaacctgattcaatagagtccctcccagtcgcgatcgtcattcattctcatcattctgcttaagtagagctagagctagagatctgcagagccagtccctgtctgtttgttagagaat
ttcaggaaagccagcccctctctgtttgttgttagaaaatttcagAGAGTGAGAGATGGAGACGATATTGGGTCTTCTGAAAGTCCGCGTGGTAAAGGGGAGTAACTTGGCTGTTCGGGACCGGACGAGCAGTGATCCCTATGTTGTAGTCAAGCTGGGTAATCAAACAGTTAAGACACGAGTAATTAAGAGGGACCTTA
ATCCAGTTTGGGATGAAGAATTGACGCTATCTATACCAAATCCAACTCCACCACTTAAACTGCAAGTATTTGACAAAGATACATTAAGCAGAGATGACAAGATGGGAGATGCCGTAATCGATCTGCAACCTCTAGTCATGGCTGTAAGCATGAGAAATGCGTTGCCATTAACATTGACAAGCAAATCTGAGACTGAATTG
TACAGATTGGTGGCATGCAAAGACAACTATCTTGTGAAAGACAGTTGCATTCGGCATGTTGATGGAAACACAGTTCAGGAAATATACTTGAGGCTTCAGAACGTTGAGTGTGGTGAACTGGAATTGCAGCTCAAGTGGGTTGATCTTCCCCGGGATTAGGAATAGGAATATCAGATCAGCTAGCCAGGCCGACAGTGTCT
CAGCATGTACACTTCATATTACCTTTGCAAGGCTTTGGCTTCCATATCTTTTCAAGGGTCTAAACTTAGTGCTAAATAAAATATGATCATTGGTTCAGGCTTCTGCTTTGTTTTAGTCAAAGGATAAGAAGAGGCATAGACTGATTCAGACATCATAAATACATAAATATATTTGCATTAGCACCATGTACATGAATTTA
TCGTACTGCGTTGCACAGGGTATATCTAAACAACCTTCATAAATGTTGAGTATAACTAAGTTTTCTAATTTTTTCTCTGAAATGCTATCATTACTTTCTCTTAGAGCAGGAGATATGTAAACAACCTTCATAGTTGTGTAGCATAATTTAGTTTTAGTGCATCTTCATTGTAGTTACACACATTCATATGACTCATGGTA
AAGTTGGCATCCAAGAGACAGAAACTCCTTTCTAGATATTATCTTTATAATAACAACTTATTGCACCAAGAATAGTGAGGTTTTCTACAGCCCAATATGATATAGATGATGTCATGCATATAATACTTATATGGTGGCTTAAAAAACACAATACTTATAATAAAACATCTCGAC

Intron 2 GTAACCAACT ... AAAATTTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	82.35	27	1	126
A2	84.87	23	0	129
A3	80	49	1	196
A4	80.73	21	3	88
A5	84.21	9	3	48
A6	82.69	108	5	516
A7	83.31	138	0	689
A8	85.46	73	5	429
A9	82.75	124	10	595
A10	81.86	127	1	573

>TRINITY_DN37007_c0_g1_i2 from sample A7 / Example spliced isoform

ATGTGCCAAGCCGGGCTTTGCGGATGAGCTTTCCCTGTCCCTGTTCCTGTTCCCTCTCCTTTTTGGAAATGAAAATGTAAAAGTGATCGTGGTATTTGGAAAATTGGAGGTAGGCTGTCCCGTCAAATCGGAGAATAAAAAGAACGTCAATTCATTGTCACAAGGCTTCATTGATGATTGGGAGGCAAGTTGGCAACCGA
ACTACAACAAACCCGTGGTGTCAACTAGAGTGAGAGATGGAGACGATATTGGGTCTTCTGAAAGTCCGCGTGGTAAAGGGGAGTAACTTGGCTGTTCGGGACCGGACGAGCAGTGATCCCTATGTTGTAGTCAAGCTGGGTAATCAAACAGTTAAGACACGAGTAATTAAGAGGGACCTTAATCCAGTTTGGGATGAAGA
ATTGACGCTATCTATACCAAATCCAACTCCACCACTTAAACTGCAAGTATTTGACAAAGATACATTAAGCAGAGATGACAAGATGGGAGATGCCGTAATCGATCTGCAACCTCTAGTCATGGCTGTAAGCATGAGAAATGCGTTGCCATTAACATTGACAAGCAAATCTGAGACTGAATTGTACAGATTGGTGGCATGCA
AAGACAACTATCTTGTGAAAGACAGTTGCATTCGGCATGTTGATGGAAACACAGTTCAGGAAATATACTTGAGGCTTCAGAACGTTGAGTGTGGTGAACTGGAATTGCAGCTCAAGTGGGTTGATCTTCCCCGGGATTAGGAATAGGAATATCAGATCAGCTAGCCAGGCCGACAGTGTCTCAGCATGTACACTTCATAT
TACCTTTGCAAGGCTTTGGCTTCCATATCTTTTCAAGGGTCTAAACTTAGTGCTAAATAAAATATGATCATTGGTTCAGGCTTCTGCTTTGTTTTAGTCAAAGGATAAGAAGAGGCATAGACTGATTCAGACATCATAAATACATAAATATATTTGCATTAGCACCATGTACATGAATTTATCGTACTGCGTTGCACAGG
GTATATCTAAACAACCTTCATAAATGTTGAGTATAACTAAGTTTTCTAATTTTTTCTCTGAAATGCTATCATTACTTTCTCTTAGAGCAGGAGATATGTAAACAACCTTCATAGTTGTGTAGCATAATTTAGTTTTAGTGCATCTTCATTGTAGTTACACACATTCATATGACTCATGGTAAAGTTGGCATCCAAGAGAC
AGAAACTCCTTTCTAGATATTATCTTTATAATAACAACTTATTGCACCAAGAATAGTGAGGTTTTCTACAGCCCAATATGATATAGATGATGTCATGCATATAATACTTATATGGTGGCTTAAAAAACACAATACTTATAATAAAACATCTCGAC

>TRINITY_DN37007_c0_g1_i1 from sample A7 / Example unspliced isoform:

ATGTGCCAAGCCGGGCTTTGCGGATGAGCTTTCCCTGTCCCTGTTCCTGTTCCCTCTCCTTTTTGGAAATGAAAATGTAAAAGTGATCGTGGTATTTGGAAAATTGGAGGTAGGCTGTCCCGTCAAATCGGAGAATAAAAAGAACGTCAATTCATTGTCACAAGGCTTCATTGATGATTGGGAGGCAAGTTGGCAACCGA
ACTACAACAAACCCGTGGTGTCAACTgtaaccaacttttcacttattcaacggcagagtaattttgttaagactaatttattaaacctgattcaatagagtccctcccagtcgcgatcgtcattcattctcatcattctgcttaagtagagctagagctagagatctgcagagccagtccctgtctgtttgttagagaat
ttcaggaaagccagcccctctctgtttgttgttagaaaatttcagAGAGTGAGAGATGGAGACGATATTGGGTCTTCTGAAAGTCCGCGTGGTAAAGGGGAGTAACTTGGCTGTTCGGGACCGGACGAGCAGTGATCCCTATGTTGTAGTCAAGCTGGGTAATCAAACAGTTAAGACACGAGTAATTAAGAGGGACCTTA
ATCCAGTTTGGGATGAAGAATTGACGCTATCTATACCAAATCCAACTCCACCACTTAAACTGCAAGTATTTGACAAAGATACATTAAGCAGAGATGACAAGATGGGAGATGCCGTAATCGATCTGCAACCTCTAGTCATGGCTGTAAGCATGAGAAATGCGTTGCCATTAACATTGACAAGCAAATCTGAGACTGAATTG
TACAGATTGGTGGCATGCAAAGACAACTATCTTGTGAAAGACAGTTGCATTCGGCATGTTGATGGAAACACAGTTCAGGAAATATACTTGAGGCTTCAGAACGTTGAGTGTGGTGAACTGGAATTGCAGCTCAAGTGGGTTGATCTTCCCCGGGATTAGGAATAGGAATATCAGATCAGCTAGCCAGGCCGACAGTGTCT
CAGCATGTACACTTCATATTACCTTTGCAAGGCTTTGGCTTCCATATCTTTTCAAGGGTCTAAACTTAGTGCTAAATAAAATATGATCATTGGTTCAGGCTTCTGCTTTGTTTTAGTCAAAGGATAAGAAGAGGCATAGACTGATTCAGACATCATAAATACATAAATATATTTGCATTAGCACCATGTACATGAATTTA
TCGTACTGCGTTGCACAGGGTATATCTAAACAACCTTCATAAATGTTGAGTATAACTAAGTTTTCTAATTTTTTCTCTGAAATGCTATCATTACTTTCTCTTAGAGCAGGAGATATGTAAACAACCTTCATAGTTGTGTAGCATAATTTAGTTTTAGTGCATCTTCATTGTAGTTACACACATTCATATGACTCATGGTA
AAGTTGGCATCCAAGAGACAGAAACTCCTTTCTAGATATTATCTTTATAATAACAACTTATTGCACCAAGAATAGTGAGGTTTTCTACAGCCCAATATGATATAGATGATGTCATGCATATAATACTTATATGGTGGCTTAAAAAACACAATACTTATAATAAAACATCTCGAC

Intron 3 GTAGGCTGTC ... AAAATTTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	78.75	34	2	126
A2	79.14	34	1	129
A3	79.35	51	4	196
A4	75.86	28	3	88
A5	80	12	3	48
A6	79.02	137	6	516
A7	79.2	181	20	689
A8	79.89	108	0	429
A9	79.12	157	21	595
A10	78.07	161	6	573

>TRINITY_DN35812_c0_g1_i2 from sample A9 / Example spliced isoform

CGAGAATAGAATATGATTTCTGTAAGAAAATGCAAGGTGGTTGCTCGTTTGTATCATGTGCCAAGCCGGGCTTTGCGGATGAGCTTTCCCTGTCCCTGTTCCTGTTCCCTCTCCTTTTTTGGAAATGAAAATGTAAAAGTGATCGTGGTATTTGGAAAATTGGAGAGAGTGAGAGATGGAGACGATATTGGGTCTTCTGA
AAGTCCGCGTGGTAAAGGGGAGTAACTTGGCTGTTCGGGACCGGACGAGCAGTGATCCCTATGTTGTAGTCAAGCTGGGTAATCAAACAGTTAAGACACGAGTAATTAAGAGGGACCTTAATCCAGTTTGGGATGAAGAATTGACGCTATCTATACCAAATCCAACTCCACCACTTAAACTGCAAGTATTTGACAAAGAT
ACATTAAGCAGAGATGACAAGATGGGAGATGCCGTAATCGATCTGCAACCTCTAGTCATGGCTGTAAGCATGAGAAATGCGTTGCCATTAACATTGACAAGCAAATCTGAGACTGAATTGTACAGATTGGTGGCATGCAAAGACAACTATCTTGTGAAAGACAGTTGCATTCGGCATGTTGATGGAAACACAGTTCAGGA
AATATACTTGAGGCTTCAGAACGTTGAGTGTGGTGAACTGGAATTGCAGCTCAAGTGGGTTGATCTTCCCCGGGATTAGGAATAGGAATATCAGATCAGCTAGCCAGGCCGACAGTGTCTCAGCATGTACACTTCATATTACCTTTGCAAGGCTTTGGCTTCCATATCTTTTCAAGGGTCTAAACTTAGTGCTAAATAAA
ATATGATCATTGGTTCAGGCTTCTGCTTTGTTTTAGTCAAAGGATAAGAAGAGGCATAGACTGATTCAGACATCATAAATACATAAATATATTTGCATTAGCACCATGTACATGAATTTATCGTACTGCGTTGCACAGGGTATATCTAAACAACCTTCATAAATGTTGAGTATAACTAAGTTTTCTAATTTTTTCTCTGA
AATGCTATCATTACTTTCTCTTAGAGCAGGAGATATGTAAACAACCTTCATAGTTGTGTAGCATAATTTAGTTTTAGTGCATCTTCATTGTAGTTACACACATTCATATGACTCATGGTAAAGTTGGCATCCAAGAGACAGAAACTCCTTTCTAGATATTATCTTTATAATAACAACTTATTGCACCAAGAATAGTGAG

>TRINITY_DN35812_c0_g1_i3 from sample A9 / Example unspliced isoform:

CGAGAATAGAATATGATTTCTGTAAGAAAATGCAAGGTGGTTGCTCGTTTGTATCATGTGCCAAGCCGGGCTTTGCGGATGAGCTTTCCCTGTCCCTGTTCCTGTTCCCTCTCCTTTTTGGAAATGAAAATGTAAAAGTGATCGTGGTATTTGGAAAATTGGAGgtaggctgtcccgtcaaatcggagaataaaaagaac
gtcaattcattgtcacaaggcttcattgatgattgggaggcaagttggcaaccgaactacaacaaacccgtggtgtcaactgtaaccaacttttcacttattcaacggcagagtaattttgttaagactaatttattaaacctgattcaatagagtccctcccagtcgcgatcgtcattcattctcatcattctgcttaa
gtagagctagagctagagatctgcagagccagtccctgtctgtttgttagagaatttcaggaaagccagcccctctctgtttgttgttagaaaatttcagAGAGTGAGAGATGGAGACGATATTGGGTCTTCTGAAAGTCCGCGTGGTAAAGGGGAGTAACTTGGCTGTTCGGGACCGGACGAGCAGTGATCCCTATGTT
GTAGTCAAGCTGGGTAATCAAACAGTTAAGACACGAGTAATTAAGAGGGACCTTAATCCAGTTTGGGATGAAGAATTGACGCTATCTATACCAAATCCAACTCCACCACTTAAACTGCAAGTATTTGACAAAGATACATTAAGCAGAGATGACAAGATGGGAGATGCCGTAATCGATCTGCAACCTCTAGTCATGGCTGT
AAGCATGAGAAATGCGTTGCCATTAACATTGACAAGCAAATCTGAGACTGAATTGTACAGATTGGTGGCATGCAAAGACAACTATCTTGTGAAAGACAGTTGCATTCGGCATGTTGATGGAAACACAGTTCAGGAAATATACTTGAGGCTTCAGAACGTTGAGTGTGGTGAACTGGAATTGCAGCTCAAGTGGGTTGATC
TTCCCCGGGATTAGGAATAGGAATATCAGATCAGCTAGCCAGGCCGACAGTGTCTCAGCATGTACACTTCATATTACCTTTGCAAGGCTTTGGCTTCCATATCTTTTCAAGGGTCTAAACTTAGTGCTAAATAAAATATGATCATTGGTTCAGGCTTCTGCTTTGTTTTAGTCAAAGGATAAGAAGAGGCATAGACTGAT
TCAGACATCATAAATACATAAATATATTTGCATTAGCACCATGTACATGAATTTATCGTACTGCGTTGCACAGGGTATATCTAAACAACCTTCATAAATGTTGAGTATAACTAAGTTTTCTAATTTTTTCTCTGAAATGCTATCATTACTTTCTCTTAGAGCAGGAGATATGTAAACAACCTTCATAGTTGTGTAGCATA
ATTTAGTTTTAGTGCATCTTCATTGTAGTTACACACATTCATATGACTCATGGTAAAGTTGGCATCCAAGAGACAGAAACTCCTTTCTAGATATTATCTTTATAATAACAACTTATTGCACCAAGAATAGTGAG