NA ----> NA

Intron 1 GTGAGAGAAA ... TCAATCTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	78.26	5	14	22
A2	71.43	14	25	45
A3	71.43	8	14	26
A4	51.43	17	13	23
A5	88.89	1	7	9
A6	45.45	21	10	25
A7	75	5	15	15
A8	57.89	4	6	5
A9	60	10	14	16
A10	68.42	9	17	22

>TRINITY_DN48836_c0_g3_i2 from sample A7 / Example spliced isoform

TGCCCATCAAATTCCATCACTGCTTGGCACCACCGTCCACCTGTCGCCCTGACTCACCTTCCTTCGTCGAAGCAGCTGTATTCGTACTGTATCTATCTCGTCGAGTGTACTTTTGTCTCTAGCAGTTTCTGGAGGAAGAGTTAACAACATTCTCGTCTCACTTTGTTGTTTGATTGCTATTAACACGAGTTCTGGAGTCT
CGGCATGAACAAACCCTATGGAGATTTCATAAGCAAAATACATTATGTTTACTCTTCGAATCATTCATTACAGGAAAGCAAGCTTAAGTTGAATTAACAGCTTGAATGTATTTCTGGAGCTTGTTTAAGGTTCACCGTTGTCCTGTTTGGGCTTACCCATCGGCGATTCTTATGCAGGTATACACCATTGTTCTGAGTTA
TGCATTTGATCTATGATGATTTCCTATTGGCAAATACACCAACTTACTCTTCCGTTTGGAAATGCACGGCGCGCAACACGTTACCAGGGTCTTTCTTCAATCCGCCTTTGGTTGACATTTCTCTATAGAGGTGACGACGAAGAACTCGGATGGCTGCAACGAACAGTCTCTAAAAGACCTGGTTATTTCTATAATCATAT
AGGTTTAAATGTATGTAAATTTTGAAAACAAGTTTTAAAAAAATTATGGCTAGTCTTTCCTTTACTAGTACTGGTCCCGAACCCTGAAGGGTTTCTGCCCTCCTTTCTCCTTTTTGTTTGAAGAACAAGA

>TRINITY_DN48836_c0_g3_i1 from sample A7 / Example unspliced isoform:

TGCCCATCAAATTCCATCACTGCTTGGCACCACCGTCCACCTGTCGCCCTGACTCACCTTCCTTCGTCGAAGCAGCTGTATTCGTACTGTATCTATCTCGTCGAGTGTACTTTTGTCTCTAGCAGTTTCTGGAGGAAGAGTTAACAACATTCTCGTCTCACTTTGTTGTTTGATTGCTATTAACACGAGTTCTGGAGTCT
CGGCATGAACAAACCCTATGGAGATTTCATAAGCAAAATACATTATGTTTACTCTTCGAATCATTCATTACAGGAAAGCAAGCTTAAGTTGAATTAACAGCTTGAATGTATTTCTGGAGCTTGTTTAAGGTTCACCGTTGTCCTGTTTGGGCTTACCCATCGGCGATTCTTATGCAGgtgagagaaaataatgaccagac
tcgagaatcctttatctgcttggcatgtttcagttaatgtagtctaagtattcaatctcagGTATACACCATTGTTCTGAGTTATGCATTTGATCTATGATGATTTCCTATTGGCAAATACACCAACTTACTCTTCCGTTTGGAAATGCACGGCGCGCAACACGTTACCAGGGTCTTTCTTCAATCCGCCTTTGGTTGAC
ATTTCTCTATAGGTATGTTGTAAACATTTCAAGCCGAGGCTTGGGAAAGAGGCATTGAGAATCGATTAAGGGTTTAACCATTTTCTGAACTTTAAAGAATTTGCATGTAGAGGTGACGACGAAGAACTCGGATGGCTGCAACGAACAGTCTCTAAAAGACCTGGTTATTTCTATAATCATATAGGTTTAAATGTATGTAA
ATTTTGAAAACAAGTTTTAAAAAAATTATGGCTAGTCTTTCCTTTACTAGTACTGGTCCCGAACCCTGAAGGGTTTCTGCCCTCCTTTCTCCTTTTTGTTTGAAGAACAAGA

Intron 2 GTATGTTGTA ... TTGCATGTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	100	0	28	10
A2	100	0	19	13
A3	100	0	10	3.7
A4	90.31	1	10	9
A5	100	0	9	5.3
A6	86.81	2	14	12.3
A7	82.09	3	14	13.7
A8	90.75	2	24	15.3
A9	100	0	12	12.3
A10	90.51	1	8	11.7

>TRINITY_DN41450_c2_g2_i15 from sample A9 / Example spliced isoform

GCAAAACGCCCCAGCATTTTGCCCATCAAATTCCATCACTGCTTGGCACCACCGTCCACCTGTCGCCCTGACTCACCTTCCTTCGTCGAAGCAGCTGTATTCGTACTGTATCTATCTCGTCGAGTGTACTTTTGTCTCTAGCAGTTTCTGGAGGAAGAGTTAACAACATTCTCGTCTCACTTTGTTGTTTGATTGCTATT
AACACGAGTTCTGGAGTCTCGGCATGAACAAACCCTATGGAGATTTCATAAGCAAAATACATTATGTTTACTCTTCGAATCATTCATTACAGGTGAGAGAAAATAATGACCAGACTCGAGAATCCTTTATCTGCTTGGCATGTTTCAGTTAATGTAGTCTAAGTATTCAATCTCAGGGTCTTTCTTCAATCCGCCTTTGG
TTGACATTTCTCTATAGAGGTGACGACGAAGAACTCG

>TRINITY_DN41450_c2_g2_i2 from sample A9 / Example unspliced isoform:

GCAAAACGCCCCAGCATTTTGCCCATCAAATTCCATCACTGCTTGGCACCACCGTCCACCTGTCGCCCTGACTCACCTTCCTTCGTCGAAGCAGCTGTATTCGTACTGTATCTATCTCGTCGAGTGTACTTTTGTCTCTAGCAGTTTCTGGAGGAAGAGTTAACAACATTCTCGTCTCACTTTGTTGTTTGATTGCTATT
AACACGAGTTCTGGAGTCTCGGCATGAACAAACCCTATGGAGATTTCATAAGCAAAATACATTATGTTTACTCTTCGAATCATTCATTACAGGAAAGCAAGCTTAAGTTGAATTAACAGCTTGAATGTATTTCTGGAGCTTGTTTAAGGTACACCGTTGTCCTGTTTGGGCTTACCCATCGGCGATTCTTATGCAGGTGA
GAGAAAATAATGACCAGACTCGAGAATCCTTTATCTGCTTGGCATGTTTCAGTTAATGTAGTCTAAGTATTCAATCTCAGGTATACACCATTGTTCTGAGTTATGCATTTGATCTATGATGATTTCCTATTGGCAAATACACCAACTTACTCTTCCGTTTGGAAATGCACGGCGCGCAACACGTTACCAGGGTCTTTCTT
CAATCCGCCTTTGGTTGACATTTCTCTATAGgtatgttgtaaacatttcaagccgaggcttgggaaagaggcattgagaatcgattaagggtttaaccattttctgaactttaaagaattgcatgtagAGGTGACGACGAAGAACTCGGATGGCTGCAACGAACAGTCTCTAAAAGACCTGGTTATTTCTATAATCATAT
AGGTTTAAATGTTTGTGGATAATTTGGGTTTATGTGGTCAACAATTGGACACACTCTGCAAGAATTCATTGGGATTCCCTGATGTCAT

Intron 3 GTTATTTCTA ... TTGGAAGGAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	55	5	4	8
A2	66.66	0.5	0	1
A3	100	0	3	5
A4	85.91	1.5	7	11
A5	71.43	1	2	3
A6	60	1	2	1
A7	63.64	2	4	3
A8	37.56	7.5	6	3
A9	58.44	4	5	5
A10	81.82	1	6	3

>TRINITY_DN41450_c2_g2_i11 from sample A9 / Example spliced isoform

GCAAAACGCCCCAGCATTTTGCCCATCAAATTCCATCACTGCTTGGCACCACCGTCCACCTGTCGCCCTGACTCACCTTCCTTCGTCGAAGCAGCTGTATTCGTACTGTATCTATCTCGTCGAGTGTACTTTTGTCTCTAGCAGTTTCTGGAGGAAGAGTTAACAACATTCTCGTCTCACTTTGTTGTTTGATTGCTATT
AACACGAGTTCTGGAGTCTCGGCATGAACAAACCCTATGGAGATTTCATAAGCAAAATACATTATGTTTACTCTTCGAATCATTCATTACAGGAAAGCAAGCTTAAGTTGAATTAACAGCTTGAATGTATTTCTGGAGCTTGTTTAAGGTACACCGTTGTCCTGTTTGGGCTTACCCATCGGCGATTCTTATGCAGGTGA
GAGAAAATAATGACCAGACTCGAGAATCCTTTATCTGCTTGGCATGTTTCAGTTAATGTAGTCTAAGTATTCAATCTCAGGTATACACCATTGTTCTGAGTTATGCATTTGATCTATGATGATTTCCTATTGGCAAATACACCAACTTACTCTTCCGTTTGGAAATGCACGGCGCGCAACACGTTACCAGGGTCTTTCTT
CAATCCGCCTTTGGTTGACATTTCTCTATAGGTATGTTGTAAACATTTCAAGCCGAGGCTTGGGAAAGAGGCATTGAGAATCGATTAAGGGTTTAACCATTTTCTGAACTTTAAAGAATTGCATGTAGAGGTGACGACGAAGAACTCGGATGGCTGCAACGAACAGTCTCTAAAAGACCTGACTGAACGGTGGCGAGAGA
TTGAATATGAGGAGAGCAGTCCAAATGAATCCGTTGCCAAGGGCTTGGAATTCCGAAATCTCAAGGAGCAATGCTTCAACTAAACCATCAGGGTGAATTTCAAATGGAATCCTTATTGGGGCAATGGTGATAA

>TRINITY_DN41450_c2_g2_i5 from sample A9 / Example unspliced isoform:

GCAAAACGCCCCAGCATTTTGCCCATCAAATTCCATCACTGCTTGGCACCACCGTCCACCTGTCGCCCTGACTCACCTTCCTTCGTCGAAGCAGCTGTATTCGTACTGTATCTATCTCGTCGAGTGTACTTTTGTCTCTAGCAGTTTCTGGAGGAAGAGTTAACAACATTCTCGTCTCACTTTGTTGTTTGATTGCTATT
AACACGAGTTCTGGAGTCTCGGCATGAACAAACCCTATGGAGATTTCATAAGCAAAATACATTATGTTTACTCTTCGAATCATTCATTACAGGTGAGAGAAAATAATGACCAGACTCGAGAATCCTTTATCTGCTTGGCATGTTTCAGTTAATGTAGTCTAAGTATTCAATCTCAGGTATACACCATTGTTCTGAGTTAT
GCATTTGATCTATGATGATTTCCTATTGGCAAATACACCAACTTACTCTTCCGTTTGGAAATGCACGGCGCGCAACACGTTACCAGGGTCTTTCTTCAATCCGCCTTTGGTTGACATTTCTCTATAGGTATGTTGTAAACATTTCAAGCCGAGGCTTGGGAAAGAGGCATTGAGAATCGATTAAGGGTTTAACCATTTTC
TGAACTTTAAAGAATTTGCATGTAGAGGTGACGACGAAGAACTCGGATGGCTGCAACGAACAGTCTCTAAAAGACCTGgttatttctataatcatataggtttaaatcctacctgagaaagacttgatagtacgaaatgcaatccaacaagcatattaagtgatctaatctgtttggaaggagACTGAACGGTGGCGAGA
GATTGAATATGAGGAGAGCAGTCCAAATGAATCCGTTGCCAAGGGCTTGGAATTCCGAAATCTCAAGGAGCAATGGTTTGTGGATAATTTGGGTTTATGTGGTCAACAATTGGACACACTCTGCAAGAATTCATTGGGATTCCCTGATGTCAT

Intron 4 GTTATTTCTA ... TTGGAAGGAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	66.97	5	8	12
A2	92.31	0.5	10	1
A3	100	0	20	7
A4	92.74	1.5	25	13
A5	86.67	1	10	3
A6	84.62	1	9	2
A7	84	2	18	3
A8	62.54	7.5	22	3
A9	65.81	4	10	4
A10	90.91	1	17	3

>TRINITY_DN41450_c2_g2_i11 from sample A9 / Example spliced isoform

GCAAAACGCCCCAGCATTTTGCCCATCAAATTCCATCACTGCTTGGCACCACCGTCCACCTGTCGCCCTGACTCACCTTCCTTCGTCGAAGCAGCTGTATTCGTACTGTATCTATCTCGTCGAGTGTACTTTTGTCTCTAGCAGTTTCTGGAGGAAGAGTTAACAACATTCTCGTCTCACTTTGTTGTTTGATTGCTATT
AACACGAGTTCTGGAGTCTCGGCATGAACAAACCCTATGGAGATTTCATAAGCAAAATACATTATGTTTACTCTTCGAATCATTCATTACAGGAAAGCAAGCTTAAGTTGAATTAACAGCTTGAATGTATTTCTGGAGCTTGTTTAAGGTACACCGTTGTCCTGTTTGGGCTTACCCATCGGCGATTCTTATGCAGGTGA
GAGAAAATAATGACCAGACTCGAGAATCCTTTATCTGCTTGGCATGTTTCAGTTAATGTAGTCTAAGTATTCAATCTCAGGTATACACCATTGTTCTGAGTTATGCATTTGATCTATGATGATTTCCTATTGGCAAATACACCAACTTACTCTTCCGTTTGGAAATGCACGGCGCGCAACACGTTACCAGGGTCTTTCTT
CAATCCGCCTTTGGTTGACATTTCTCTATAGGTATGTTGTAAACATTTCAAGCCGAGGCTTGGGAAAGAGGCATTGAGAATCGATTAAGGGTTTAACCATTTTCTGAACTTTAAAGAATTGCATGTAGAGGTGACGACGAAGAACTCGGATGGCTGCAACGAACAGTCTCTAAAAGACCTGACTGAACGGTGGCGAGAGA
TTGAATATGAGGAGAGCAGTCCAAATGAATCCGTTGCCAAGGGCTTGGAATTCCGAAATCTCAAGGAGCAATGCTTCAACTAAACCATCAGGGTGAATTTCAAATGGAATCCTTATTGGGGCAATGGTGATAA

>TRINITY_DN41450_c2_g2_i3 from sample A9 / Example unspliced isoform:

GCAAAACGCCCCAGCATTTTGCCCATCAAATTCCATCACTGCTTGGCACCACCGTCCACCTGTCGCCCTGACTCACCTTCCTTCGTCGAAGCAGCTGTATTCGTACTGTATCTATCTCGTCGAGTGTACTTTTGTCTCTAGCAGTTTCTGGAGGAAGAGTTAACAACATTCTCGTCTCACTTTGTTGTTTGATTGCTATT
AACACGAGTTCTGGAGTCTCGGCATGAACAAACCCTATGGAGATTTCATAAGCAAAATACATTATGTTTACTCTTCGAATCATTCATTACAGGAAAGCAAGCTTAAGTTGAATTAACAGCTTGAATGTATTTCTGGAGCTTGTTTAAGGTACACCGTTGTCCTGTTTGGGCTTACCCATCGGCGATTCTTATGCAGGTAT
ACACCATTGTTCTGAGTTATGCATTTGATCTATGATGATTTCCTATTGGCAAATACACCAACTTACTCTTCCGTTTGGAAATGCACGGCGCGCAACACGTTACCAGGGTCTTTCTTCAATCCGCCTTTGGTTGACATTTCTCTATAGGTATGTTGTAAACATTTCAAGCCGAGGCTTGGGAAAGAGGCATTGAGAATCGA
TTAAGGGTTTAACCATTTTCTGAACTTTAAAGAATTTGCATGTAGAGGTGACGACGAAGAACTCGGATGGCTGCAACGAACAGTCTCTAAAAGACCTGgttatttctataatcatataggtttaaatgtatgtaaattttgaaaacaagttttaaaaaaattatggctagtctttcctttactagtactggtcccgaacc
ctgaagggtttctgccctcctttctcctttttgtttgaagaacaagagtatatatatatatatatttcagcatttaaatttttgcaagtatttcttactgatattttgaacgcgtaggtaatttatgatatctctgtataggtatgaataagggttctcaattcattatcccaagttgaaggctttataagtcaagacat
atcaatcgatcttcatttacaggatttcctttttttaatatgactacaattgtttatctaatttgaatacccgcttaagggctactcttcctagagccgacttggcttctctgactcgttttttttaatctttaaactagactgtcctatatttttcttttatttacaaccctctttctttttttgttgtcaaaaatcaa
ctacttggtcttaagcctacctgagaaagacttgatagtacgaaatgcaatccaacaagcatattaagtgatctaatctgtttggaaggagACTGAACGGTGGCGAGAGATTGAATATGAGGAGAGCAGTCCAAATGAATCCGTTGCCAAGGGCTTGGAATTCCGAAATCTCAAGGAGCAATGGTTTGTGGATAATTTGG
GTTTATGTGGTCAACAATTGGACACACTCTGCAAGAATTCATTGGGATTCCCTGATGTCAT

Intron 5 GTATACACCA ... ACGTTACCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	88.05	3.3	22.2	25
A2	90.43	3.7	44.2	22
A3	96	1	25.8	18
A4	90.1	2.3	22.5	17
A5	71.06	4.2	9.5	10
A6	82.67	4.8	25.3	20
A7	69.42	4.3	13.5	5
A8	72.05	4.3	5.2	16
A9	80.32	3.2	16.5	9
A10	70.39	6	21.8	6

>TRINITY_DN48836_c0_g3_i3 from sample A7 / Example spliced isoform

GAGAGAAAATAATGACCAGACTCGAGAATCCTTTATCTGCTTGGCATGTTTCAGTTAATGTAGTCTAAGTATTCAATCTCAGGGTCTTTCTTCAATCCGCCTTTGGTTGACATTTCTCTATAGGTATGTTGTAAACATTTCAAGCCGAGGCTTGGGAAAGAGGCATTGAGAATCGATTAAGGGTTTAACCATTTTCTGAA
CTTTAAAGAATTTGCATGTAGAGGTGACGACGAAGAACTCGGATGGCTGCAACGAACAGTCTCTAAAAGACCTGGTTATTTCTATAATCATATAGGTTTAAATGTATGTAAATTTTGAAAACAAGTTTTAAAAAAATTATGGCTAGTCTTTCCTTTACTAGTACTGGTCCCGAACCCTGAAGGGTTTCTGCCCTCCTTTC
TCCTTTTTGTTTGAAGAACAAGA

>TRINITY_DN48836_c0_g3_i1 from sample A7 / Example unspliced isoform:

TGCCCATCAAATTCCATCACTGCTTGGCACCACCGTCCACCTGTCGCCCTGACTCACCTTCCTTCGTCGAAGCAGCTGTATTCGTACTGTATCTATCTCGTCGAGTGTACTTTTGTCTCTAGCAGTTTCTGGAGGAAGAGTTAACAACATTCTCGTCTCACTTTGTTGTTTGATTGCTATTAACACGAGTTCTGGAGTCT
CGGCATGAACAAACCCTATGGAGATTTCATAAGCAAAATACATTATGTTTACTCTTCGAATCATTCATTACAGGAAAGCAAGCTTAAGTTGAATTAACAGCTTGAATGTATTTCTGGAGCTTGTTTAAGGTTCACCGTTGTCCTGTTTGGGCTTACCCATCGGCGATTCTTATGCAGGTGAGAGAAAATAATGACCAGAC
TCGAGAATCCTTTATCTGCTTGGCATGTTTCAGTTAATGTAGTCTAAGTATTCAATCTCAGgtatacaccattgttctgagttatgcatttgatctatgatgatttcctattggcaaatacaccaacttactcttccgtttggaaatgcacggcgcgcaacacgttaccagGGTCTTTCTTCAATCCGCCTTTGGTTGAC
ATTTCTCTATAGGTATGTTGTAAACATTTCAAGCCGAGGCTTGGGAAAGAGGCATTGAGAATCGATTAAGGGTTTAACCATTTTCTGAACTTTAAAGAATTTGCATGTAGAGGTGACGACGAAGAACTCGGATGGCTGCAACGAACAGTCTCTAAAAGACCTGGTTATTTCTATAATCATATAGGTTTAAATGTATGTAA
ATTTTGAAAACAAGTTTTAAAAAAATTATGGCTAGTCTTTCCTTTACTAGTACTGGTCCCGAACCCTGAAGGGTTTCTGCCCTCCTTTCTCCTTTTTGTTTGAAGAACAAGA

Intron 6 GTATGTAAAT ... TGGTCTTAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	80.65	3	7	18
A2	87.5	1	10	4
A3	80.65	3	20	5
A4	64.52	11	24	16
A5	71.43	3	10	5
A6	86.67	1	12	1
A7	73.91	3	16	1
A8	81.25	3	25	1
A9	56.52	5	12	1
A10	65.52	5	17	2

>TRINITY_DN41450_c2_g2_i5 from sample A9 / Example spliced isoform

GCAAAACGCCCCAGCATTTTGCCCATCAAATTCCATCACTGCTTGGCACCACCGTCCACCTGTCGCCCTGACTCACCTTCCTTCGTCGAAGCAGCTGTATTCGTACTGTATCTATCTCGTCGAGTGTACTTTTGTCTCTAGCAGTTTCTGGAGGAAGAGTTAACAACATTCTCGTCTCACTTTGTTGTTTGATTGCTATT
AACACGAGTTCTGGAGTCTCGGCATGAACAAACCCTATGGAGATTTCATAAGCAAAATACATTATGTTTACTCTTCGAATCATTCATTACAGGTGAGAGAAAATAATGACCAGACTCGAGAATCCTTTATCTGCTTGGCATGTTTCAGTTAATGTAGTCTAAGTATTCAATCTCAGGTATACACCATTGTTCTGAGTTAT
GCATTTGATCTATGATGATTTCCTATTGGCAAATACACCAACTTACTCTTCCGTTTGGAAATGCACGGCGCGCAACACGTTACCAGGGTCTTTCTTCAATCCGCCTTTGGTTGACATTTCTCTATAGGTATGTTGTAAACATTTCAAGCCGAGGCTTGGGAAAGAGGCATTGAGAATCGATTAAGGGTTTAACCATTTTC
TGAACTTTAAAGAATTTGCATGTAGAGGTGACGACGAAGAACTCGGATGGCTGCAACGAACAGTCTCTAAAAGACCTGGTTATTTCTATAATCATATAGGTTTAAATCCTACCTGAGAAAGACTTGATAGTACGAAATGCAATCCAACAAGCATATTAAGTGATCTAATCTGTTTGGAAGGAGACTGAACGGTGGCGAGA
GATTGAATATGAGGAGAGCAGTCCAAATGAATCCGTTGCCAAGGGCTTGGAATTCCGAAATCTCAAGGAGCAATGGTTTGTGGATAATTTGGGTTTATGTGGTCAACAATTGGACACACTCTGCAAGAATTCATTGGGATTCCCTGATGTCAT

>TRINITY_DN41450_c2_g2_i3 from sample A9 / Example unspliced isoform:

GCAAAACGCCCCAGCATTTTGCCCATCAAATTCCATCACTGCTTGGCACCACCGTCCACCTGTCGCCCTGACTCACCTTCCTTCGTCGAAGCAGCTGTATTCGTACTGTATCTATCTCGTCGAGTGTACTTTTGTCTCTAGCAGTTTCTGGAGGAAGAGTTAACAACATTCTCGTCTCACTTTGTTGTTTGATTGCTATT
AACACGAGTTCTGGAGTCTCGGCATGAACAAACCCTATGGAGATTTCATAAGCAAAATACATTATGTTTACTCTTCGAATCATTCATTACAGGAAAGCAAGCTTAAGTTGAATTAACAGCTTGAATGTATTTCTGGAGCTTGTTTAAGGTACACCGTTGTCCTGTTTGGGCTTACCCATCGGCGATTCTTATGCAGGTAT
ACACCATTGTTCTGAGTTATGCATTTGATCTATGATGATTTCCTATTGGCAAATACACCAACTTACTCTTCCGTTTGGAAATGCACGGCGCGCAACACGTTACCAGGGTCTTTCTTCAATCCGCCTTTGGTTGACATTTCTCTATAGGTATGTTGTAAACATTTCAAGCCGAGGCTTGGGAAAGAGGCATTGAGAATCGA
TTAAGGGTTTAACCATTTTCTGAACTTTAAAGAATTTGCATGTAGAGGTGACGACGAAGAACTCGGATGGCTGCAACGAACAGTCTCTAAAAGACCTGGTTATTTCTATAATCATATAGGTTTAAATgtatgtaaattttgaaaacaagttttaaaaaaattatggctagtctttcctttactagtactggtcccgaacc
ctgaagggtttctgccctcctttctcctttttgtttgaagaacaagagtatatatatatatatatttcagcatttaaatttttgcaagtatttcttactgatattttgaacgcgtaggtaatttatgatatctctgtataggtatgaataagggttctcaattcattatcccaagttgaaggctttataagtcaagacat
atcaatcgatcttcatttacaggatttcctttttttaatatgactacaattgtttatctaatttgaatacccgcttaagggctactcttcctagagccgacttggcttctctgactcgttttttttaatctttaaactagactgtcctatatttttcttttatttacaaccctctttctttttttgttgtcaaaaatcaa
ctacttggtcttaagCCTACCTGAGAAAGACTTGATAGTACGAAATGCAATCCAACAAGCATATTAAGTGATCTAATCTGTTTGGAAGGAGACTGAACGGTGGCGAGAGATTGAATATGAGGAGAGCAGTCCAAATGAATCCGTTGCCAAGGGCTTGGAATTCCGAAATCTCAAGGAGCAATGGTTTGTGGATAATTTGG
GTTTATGTGGTCAACAATTGGACACACTCTGCAAGAATTCATTGGGATTCCCTGATGTCAT

Intron 7 GTATGTTGTA ... TTGCATGTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	79.62	5	23.8	10.8
A2	96.72	0.5	17	13.8
A3	84.66	1.5	8.5	6.3
A4	83.29	2.5	9.8	13.5
A5	84.96	1.5	8.5	6
A6	77.39	4.5	12.8	16
A7	76.89	5	14.8	17
A8	84.73	4	21.8	21.5
A9	92.67	1	10.8	14
A10	79.15	4	8.8	16.8

>TRINITY_DN48836_c0_g3_i2 from sample A7 / Example spliced isoform

TGCCCATCAAATTCCATCACTGCTTGGCACCACCGTCCACCTGTCGCCCTGACTCACCTTCCTTCGTCGAAGCAGCTGTATTCGTACTGTATCTATCTCGTCGAGTGTACTTTTGTCTCTAGCAGTTTCTGGAGGAAGAGTTAACAACATTCTCGTCTCACTTTGTTGTTTGATTGCTATTAACACGAGTTCTGGAGTCT
CGGCATGAACAAACCCTATGGAGATTTCATAAGCAAAATACATTATGTTTACTCTTCGAATCATTCATTACAGGAAAGCAAGCTTAAGTTGAATTAACAGCTTGAATGTATTTCTGGAGCTTGTTTAAGGTTCACCGTTGTCCTGTTTGGGCTTACCCATCGGCGATTCTTATGCAGGTATACACCATTGTTCTGAGTTA
TGCATTTGATCTATGATGATTTCCTATTGGCAAATACACCAACTTACTCTTCCGTTTGGAAATGCACGGCGCGCAACACGTTACCAGGGTCTTTCTTCAATCCGCCTTTGGTTGACATTTCTCTATAGAGGTGACGACGAAGAACTCGGATGGCTGCAACGAACAGTCTCTAAAAGACCTGGTTATTTCTATAATCATAT
AGGTTTAAATGTATGTAAATTTTGAAAACAAGTTTTAAAAAAATTATGGCTAGTCTTTCCTTTACTAGTACTGGTCCCGAACCCTGAAGGGTTTCTGCCCTCCTTTCTCCTTTTTGTTTGAAGAACAAGA

>TRINITY_DN48836_c0_g3_i3 from sample A7 / Example unspliced isoform:

GAGAGAAAATAATGACCAGACTCGAGAATCCTTTATCTGCTTGGCATGTTTCAGTTAATGTAGTCTAAGTATTCAATCTCAGGGTCTTTCTTCAATCCGCCTTTGGTTGACATTTCTCTATAGgtatgttgtaaacatttcaagccgaggcttgggaaagaggcattgagaatcgattaagggtttaaccattttctgaa
ctttaaagaatttgcatgtagAGGTGACGACGAAGAACTCGGATGGCTGCAACGAACAGTCTCTAAAAGACCTGGTTATTTCTATAATCATATAGGTTTAAATGTATGTAAATTTTGAAAACAAGTTTTAAAAAAATTATGGCTAGTCTTTCCTTTACTAGTACTGGTCCCGAACCCTGAAGGGTTTCTGCCCTCCTTTC
TCCTTTTTGTTTGAAGAACAAGA