NA ----> NA

Intron 1 GTAAACAATT ... GTTTTTCTAG

Found issues: Low number of reads

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	100	0	2	2
A2	100	0	6	2
A3	-1	0	0	0
A4	100	0	1	2
A5	-1	0	0	0
A6	-1	0	0	0
A7	50	1	0	2
A8	20	2	0	1
A9	33.33	2	0	2
A10	25	6	0	4

>TRINITY_DN37853_c0_g1_i2 from sample A8 / Example spliced isoform

AACGATTCCAATGATGTTGTCCACTACACCAGGCGATCCCCCACTACAACGGCCTCCACACCATGAGTACCAGACCAGGAGTGCTTTGTGTGAGAGGTTGAAAGCATAAAGATTTTCTGAAGAGAACCCAGAAAATGAAGATGACATGGATGAAGATGAATTACTAAAGAACTTTAGGAAGAGGGCTAAAAATAAATGAT
GATGTATAAACTATTTTTTTTTTTACATATGCCTAACTTCGAATGAGTTCTTACATACTTTTTGGTGCATTTCCTCTAAATGAGTTATTGCATATTTTTTTGTACTCACGATAGTAAAAT

>TRINITY_DN37853_c0_g1_i1 from sample A8 / Example unspliced isoform:

TGATGTTGTCCACTACACCAGgtaaacaattacattgcagaggtttttttttaacattaccaaactacttcaataatatttaaaaaatggccatataaatttaatattgtttgtttttctagGCGATCCCCCACTACAACGGCCTCCACACCATGAGTACCAGACCAGGAGTGCTTTGTGTGAGAGGTTGAAAGCATAAA
GATTTTCTGAAGAGAACCCAGAAAATGAAGATGACATGGATGAAGATGAATTACTAAAGAACTTTAGGAAGAGGGCTAAAAATAAATGATGATGTATAAACTATTTTTTTTTTTACATATGCCTAACTTCGAATGAGTTCTTACATACTTTTTGGTGCATTTCCTCTAAATGAGTTATTGCATATTTTTTTGTACTCACG
ATAGTAAAAT

Intron 2 GTAATATGGC ... TCAATTGCAG

Found issues: Low number of reads

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	100	0	1	0
A2	100	0	2	0
A3	-1	0	0	0
A4	-1	0	0	0
A5	-1	0	0	0
A6	-1	0	0	0
A7	51.92	6.5	7	2
A8	47.22	4.5	4	0
A9	0	1.5	0	0
A10	-1	0	0	0

>TRINITY_DN38970_c0_g1_i3 from sample A7 / Example spliced isoform

TTGAAGTTATAGTCATGAATTTTAATTACCCAAATATGTTAACAATATTTTTTGTACTGTGTTACAACTTTTAGGTAATTGCAGCAAATAACATAAAAAAACCTACTCTTATGCAATGCGGTGATGACTTCGAAATTGGAATCATGGAGGAAGACCTCAAGCTTGGTTACAGTTTTGACTATCAAGCTGTCGTTGAATGA
TGGAAAAGCTTGGGGCATATCCCAAGATCCAGGGTGAAGGATAAGGGGAGCAACACTGTCAACGAGTCCATAAGTACTGGAGAGCATTACGCTAATAGTGGAGTTCCTCCAAAAGATGGTGCTCCCGTGCAGCCCCACGTCGATGGTGCTAGGAATGAGGTCCACGGCGAGAATGTTGTACTTGAGGGTCCTACGCCTAA
AGAGGGGCATAGCGCAACGATTCCAACAATGTCGTCCACTACACCAGGTAAACAATTACATTACAGAGGTTTTTTTTTAACATTACCAAACTACTTTAATAATATTTAAAAACTGGCCATATAAATTTAATATTGTTTGTTTTTCTAGGCGATCCCCCACTACAACGGCCTCCACACCATGAGTACCAGACCAGGAGTGC
TTTGTGTGAGAGGTTGAAAGCATAAAGATTTTCTGAAGAGAACCCAGAAAATGAAGATGACATGGATGAAGATGAATTACTAAAGAACTTTAGGAAGAGGGCTAAAAATAAATGATGATGTATAAACTATTTTTTTTTTACATATGCCTAACTTCGAATGAGTTCTTACATACTTTTTGGTGCATTTCCTCTAAATGAGT
TATTGCATATTTTTTTGTACTCACGATAGTAAAATATGAAATATAGATAGTTTAGTACTTAATTTATCTCAACTTTGTACTTATTTTATCTCAACTTTCTTTCTTAG

>TRINITY_DN38970_c0_g1_i1 from sample A7 / Example unspliced isoform:

TTGAAGTTATAGTCATGAATTTTAATTACCCAAATATGTTAACAATATTTTTTGTACTGTGTTACAACTTTTAGGTAATTGCAGCAAATAACATAAAAAAAAACCTACTCTTATGCAATGCGGTGATGACTTCGAAATTGGAATCATGGAGGAAGACCTCAAGCTTGGTTACAGTTTTGACTATCAAGCTGTCGTTGAAT
GATGGAAAAGCTTGGGGCATATCCCAAGATCCAGTGTGAAGGATATGGTAAATAAAAATCAATCCACATTAAACTTTCATCACTTATAAAAATTCATTCGTGTTTGATAATGTTCATAAATTTAATCTATTACATCATTGTTTTAATCAGGGGAGCAACACTATCAACGATTCCATAAGTACTGGAGAGCATTACGCTAA
TAGTGGAGTTCCTCCAAAAGATGGTGCTCCCGTGCAGCCCCACGTCGATGGTGCTAGGAATGAGGTCCACGGCGgtaatatggctagatattaatatatcatatacttaataattccgtagttatagattttgcacttctattttttttacttatatatacatctaggattgtgtccttataaagttagtggttcaattg
cagAGAATGTTGTACTTGAGGGC

Intron 3 GTAAATAAAA ... TTTTAATCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	50	1	1	1
A2	-1	0	0	0
A3	33.33	1	1	0
A4	33.33	1	1	0
A5	-1	0	0	0
A6	-1	0	0	0
A7	31.43	12	5	6
A8	42.86	8	7	5
A9	20	2	0	1
A10	20	2	1	0

>TRINITY_DN37853_c0_g1_i3 from sample A8 / Example spliced isoform

AAAACAACACCTGGTCCCGCAAGGTAAATTACATGTTGAATTCAATAGCTTTAATTTTCTTTAGATATAAATAAAAGTGTGGTCCATAACATACTTGAAAATTTTGATATTAGGTTGCATGTCACTTTGAAGGAAACAAAAAAAAGGATTTCCAAACATAGTTAGCACAAGCTAGGCCAAGGGGGTTACTTGAATTTATA
GTCACGAATTTTAATTACCCAAATACGTTAACAATATTTTTTGTACTGCGTTACAACTTTTAGGTAATTGCAGCAAATAACATAAAAAAACCTACTCTTATGCAATGTGGTGATGACTTCGAAATTGGAATCATGGAGGAAGACCTCAAGCTTGGTTACAGTTTTGACTATCAAGCTGTCGTTGAATGATGGAAAAGCTT
GGGGCATATCCCAAGATCCAGGGTGAAGGATAAGGGGAGCAACACTGTCAACGAGTCCATAAGTACTGGAGAGCATTACGCTAATAGTGGAGTTCCTCCAAAAGATGGTGCTCCCGTGCAGCCCCACGTCGATGGTGCTAGGAATGAGGTCCACGGCGAGAATGTTGTACTTGAGGGTCCTACGCCTAAAGAGGGGCATA
GCGCAACGATTCCAACAATGTCGTCCACTACACCAGGCGATTCCCCACCACAACAGCCTCCACACCATGAGTACCAGACCAGGAGTGCTTTGTGTGAGAGGTTGAAAGCATAAAGATTTTCTGAAGAGAACCCAGAAAATGAAGATGACATGGATGAAGATGAATTACTAAAGAACTTTAGGAAGAGGGCTAAAAATAAA
TGATGATGTATAAACTATTTTTTTTTTTACATATGCCTAACTTCGAATGAGTTCTTACATACTTTTTGGTGCATTTCCTCTAAATGAGTTATTGCATATTTTTTTGTACTCACGATAGTAAAAT

>TRINITY_DN37853_c0_g1_i4 from sample A8 / Example unspliced isoform:

AAAACAACACCTGGTCCCGCAAGGTAAATTACATGTTGAATTCAATAGCTTTAATTTTCTTTAGATATAAATAAAAGTGTGGTCCATAACATACTTGAAAATTTTGATATTAGGTTGCATGTCACTTTGAAGGAAACAAAAAAAAGGATTTCCAAACATAGTTAGCACAAGCTAGGCCAAGGGGGTTACTTGAATTTATA
GTCACGAATTTTAATTACCCAAATACGTTAACAATATTTTTTGTACTGCGTTACAACTTTTAGGTAATTGCAGCAAATAACATAAAAAAACCTACTCTTATGCAATGTGGTGATGACTTCGAAATTGGAATCATGGAGGAAGACCTCAAGCTTGGTTACAGTTTTGACTATCAAGCTGTCGTTGAATGATGGAAAAGCTT
GGGGCATATCCCAAGATCCAGGGTGAAGGATAAGgtaaataaaaatcaatccacattaaactttcatcacttataaaaattcattcgtgtttgataatgttcataaatttaatctattacatcattgttttaatcagGGGAGCAACACTGTCAACGAGTCCATAAGTACTGGAGAGCATTACGCTAATAGTGGAGTTCCT
CCAAAAGATGGTGCTCCCGTGCAGCCCCACGTCGATGGTGCTAGGAATGAGGTCCACGGCGAGAATGTTGTACTTGAGGGTCCTACGCCTAAAGAGGGGCATAGCGCAACGATTCCAACAATGTCGTCCACTACACCAGGCGATTCCCCACCACAACAGCCTCCACACCATGAGTACCAGACCAGGAGTGCTTTGTGTGA
GAGGTTGAAAGCATAAAGATTTTCTGAAGAGAACCCAGAAAATGAAGATGACATGGATGAAGATGAATTACTAAAGAACTTTAGGAAGAGGGCTAAAAATAAATGATGATGTATAAACTATTTTTTTTTTTACATATGCCTAACTTCGAATGAGTTCTTACATACTTTTTGGTGCATTTCCTCTAAATGAGTTATTGCAT
ATTTTTTTGTACTCACGATAGTAAAAT