sp|Q9SR22|PAE5_ARATH ----> Pectin acetylesterase 5

Intron 1 GCTATTCTTA ... GGTTGCTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	74.25	77	232	212
A2	77.85	73	264	249
A3	72.99	94	268	240
A4	78.1	53	202	176
A5	73.51	71	203	191
A6	70.63	42	105	97
A7	77.39	59	213	191
A8	80.56	62	266	248
A9	74.63	52	162	144
A10	77.78	47	172	157

>TRINITY_DN130778_c2_g1_i7 from sample A1 / Example spliced isoform

AGTTCGATATTGTGACGGGTCGTCATTCACTGGAGATTTCTATAGCTGGATCCGTGGTTCAATATTGTGACGGGTTGTCATTCACTGGAGATGGTGCATTTCAGGGTAGCAAATTCCATTTGAGAGAACAAAGGATATGGAAGATATTTTGACACAGGGGATGAACAAAGCCGAACTGCAGGTGGTCTGGCCACATTTAT
TCACTGCGATTATTTCCGTCATCTTCTGCCCAAGAGTGCGGTCTGGCCACATTTATTCACTGCGATGATTTTCGTCATCTTCTGCCCAAGAGTGCTAA

>TRINITY_DN130778_c2_g1_i5 from sample A1 / Example unspliced isoform:

CATTGGGTTCTTCTCTGTACATGGAGAAATCAATAGCATTTACAGGCGTTTTATGTCATGTTCAATCACAAAATCCAGATTTCAATCACTGGGACCGTTGATATTGTGACGGGTCGTCATTCACTGGAGATTTCTATAGCTGGATCCGTGGTTCAATATTGTGACGGGTTGTCATTCACTGGAGATGGTGGGTAGCAAAT
TCCATTTGAGAGAACAAAGGATATGGAAGATATTTTGACACAGGGGATGAACAAAGCCGAACTGgctattcttaccggttgctcagCAGGTGGTCTGGCCACATTTATTCACTGCGATTATTTCCGTCATCTTCTGCCCAAGAGTGCGGTCTGGCCACATTTATTCACTGCGATGATTTTCGTCATCTTCTGCCCAAGAG
TGCTAA

Intron 2 GTAGGTTGAC ... TTTGTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	12.58	410	40	78
A2	12.9	378	43	69
A3	12.42	388	36	74
A4	9.87	370	21	60
A5	13.44	293	29	62
A6	6.27	157	3	18
A7	6.5	439	8	53
A8	5.24	452	3	47
A9	6.38	279	2	36
A10	7.69	240	4	36

>TRINITY_DN130778_c2_g1_i9 from sample A1 / Example spliced isoform

ACGCAAATAGTTGGCTCGTTCACTTGGAGGGTGGAGGGTGGTGTGGCGATATAGCCTCGTGTTTGCAGCACGTGCGCTCTTCTGCATTGGGTTCTTCTCTGTACATGGAGAAATCAATAGCATTTACAGGCGTTTTATGTCATGTTCAATCACAAAATCCAGATTTCAATAACTGGAACCGTGTTATAGTTCGATATTGT
GACGGGTCGTCATTCACTGGAGATTTCTATAGCTGGAACCGTGGTTCGATATTGTGACGGGTCATCATTCACTGGAGATGTTGGTAGCAAATTCCATTTGAGAGGACAAAAGATATGGCAGGCTGTAATGGAAGATCTTTTGGCACAGGGGATGAACAAAGCCAAACAAGCTATTCTTACTGGCTGCTCAGCATGTGTTC
TAGCCACAGCCAAATCATCATCTTCTGCCCAAGAGTGCTAATGATCTTCTGCCCACAGTCACAGTGGACTCCACATTACAG

>TRINITY_DN130778_c2_g1_i8 from sample A1 / Example unspliced isoform:

ATCATTCACTGGAGATGTTGGTAGCAAATTCCATTTGAGAGGACAAAAGATATGGCAAGCTGTAATGGAAGATCTTTTGGCACAGGGGATGAACAAAGCCAAACAAgtaggttgacctaattatctatgatgcatacatatgtatcataaaagtattggaagctatgaaaatggaatgtgatctatcttttgatttgttg
cagGCTATTCTTACTGGCTGCTCAGCATGTGTTCTAGCCACAGCCAAATCATCATCTTCTGCCCAAGAGTGCTAATGATCTTCTGCCCACAGTCACAGTGGACTCCACATTACAGGTACCCACCCCTAAACTTTACATCAATATTGAACCGACCTTAGCTTCTTCAACCGGAATGGCAGCATAGGTTTTTTTGTGACAGA
ACAGTTATGTAATCTATATGTTGAATGGTTC

Intron 3 GCTATTCTTA ... TGCTCAGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	43.4	386	320	272
A2	47.56	333	335	269
A3	47.48	344	343	279
A4	42.67	348	282	236
A5	42.29	292	242	186
A6	37.87	219	144	123
A7	39.91	490	352	299
A8	39.43	570	405	337
A9	38.39	341	232	193
A10	37.64	381	254	206

>TRINITY_DN130778_c2_g1_i20 from sample A1 / Example spliced isoform

CATTGGGTTCTTCTCTGTACATGGAGAAATCAATAGCATTTACAGGCGTTTTATGTCATGTTCAATCACAAAATCCAGATTTCAATAACTGGAACCGTGTTATGGTTTGATATTGTGACGGGTCGTCATTCACTGGAGATTTCTATAGCTGGAACCGTGGTTCGATATTGTGACGGGTTGTCATTCACTGGAGATGTTGG
GTAGCAAACTCCATTTGAGAGGACAAAAGATATGGCAAGTTGTAATGGAATATCTTTTGGAACAAGGGATGAACAAAGCCGAACAGGTGGTCTGGCCACATTTATTCACTGCGATGATTTTCGTCATCTTCTGCCCAAGAGTGCTAATGATCTTCTGCCCACAGTCACAGTGGACTCCACATTACAGGTACCCACCCCTA
AACTTTACATCAATATTGAACAGACCTTACCCTCTTCAACGGGAATGGCGGCATAGGTTTTTTTTGTGACAGAACAATTAAGTAATCTATATGTTGAATGGTTCTCTTCCAAACAGCCTTCATAACATTTGACCGTGTTTGCGGGTTTTTGTCAAACTATTTTGAAAAGTGATACTTCAGAAATGGATTTCTTTTCTATT
CATCATTGACCTTATCAGCTCGCCAACTGTCTCCATAAATGCTAAAGCAGCAAATTCTTAATCCATTTAATTTTGTAC

>TRINITY_DN130778_c2_g1_i11 from sample A1 / Example unspliced isoform:

CATTGGGTTCTTCTCTGTACATGGAGAAATCAATAGCATTTACAGGCGTTTTATGTCATGTTCAATCACAAAATCCAGATTTCAATAACTGGAACCGTGTTATGGTTTGATATTGTGACGGGTCGTCATTCACTGGAGATTTCTATAGCTGGAACCGTGGTTCGATATTGTGACGGGTTGTCATTCACTGGAGATGTTGG
GTAGCAAACTCCATTTGAGAGGACAAAAGATATGGCAAGTTGTAATGGAATATCTTTTGGAACAAGGGATGAACAAAGCCGAACAGgctattcttactggctgctcagcagGTGGTCTGGCCACATTTATTCACTGCGATGATTTTCGTCATCTTCTGCCCAAGAGTGCTAA