NA ----> NA

Intron 1 GTGAGTGTGT ... ATGTGAACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	70	3	0	7
A2	-1	0	0	0
A3	-1	0	0	0
A4	0	1	0	0
A5	-1	0	0	0
A6	28.57	5	0	2
A7	40.63	19	2	13
A8	33.33	12	1	6
A9	11.11	16	0	2
A10	50	2	0	2

>TRINITY_DN35178_c0_g1_i1 from sample A8 / Example spliced isoform

GGCAAATCAATGAAGCTCTTACATTTCGGCTCGCAGGTTTTCGCACCGAGGAGAAATACTGACTGAACAGTGCTGTTCAAGCGTCTTTATTCACATCATAAGCACAGGGAAGGCAACAACAAACAACAACAGGAAGATGAATTTCTCAGGCTCTCGTTCTTGTTTTTCCGTATCTATATTTGGGTGTGCATTTCCTATTA
TGGCTAAGCAACTTGTTGGCACTTTGAGTCGATGGAATCATGCAGAGAGATGGCTGAGGCGAAAGAGGCCGGCGGCCCGAAGCGTGCTGGGAAGTAGCAATGTGAGATAAAGATTTGGAGGTGTGGGCATCAAGGCTCTCTATGTTTCTGTGTAGAGAGCAAAAGGGGAATGAAGCATTTGGTTTGCAATGCTGTAGATT
TCGGTTCTGTAAATTCAGAGCTGCTTCTACAGCGACCTCTCCGATATATATTTATTTATTTTTCAAGTTCTCTGTTTGTTATGCGCCCGTAGGTGCAGACGAGGTTTGCACATGATGAACAGGGCTAAACGATGTTACAGCATTCTCGTTTATGACCTCCTGTTTTTTGTTCTGTAGCCGCTTGGCTTCATTATTTTCTA
CATATAACTTTGTGCAAACTTCTTCTGTACTCTTTTTAAAATTTTGATTATTACTGTGTCAAGAATCAAGTAACTATTATTATTATGATGATGATGGTCTACCGTCG

>TRINITY_DN35178_c0_g1_i2 from sample A8 / Example unspliced isoform:

GGCAAATCAATGAAGCTCTTACATTTCGGCTCGCAGGTTTTCGCACCGAGGAGAAATACTGACTGAACAGTGCTGTTCAAGCGTCTTTATTCACATCATAAGCACAGGgtgagtgtgtgttgtcttcctattttcatccttaaatccccactgggggttgggggttgcgttttttttgttgctcaacttaatgtcatgtc
atgtgaacagGAAGGCAACAACAAACAACAACAGGAAGATGAATTTCTCAGGCTCTCGTTCTTGTTTTTCCGTATCTATATTTGGGTGTGCATTTCCTATTATGGCTAAGCAACTTGTTGGCACTTTGAGTCGATGGAATCATGCAGAGAGATGGCTGAGGCGAAAGAGGCCGGCGGCCCGAAGCGTGCTGGGAAGTAGC
AATGTGAGATAAAGATTTGGAGGTGTGGGCATCAAGGCTCTCTATGTTTCTGTGTAGAGAGCAAAAGGGGAATGAAGCATTTGGTTTGCAATGCTGTAGATTTCGGTTCTGTAAATTCAGAGCTGCTTCTACAGCGACCTCTCCGATATATATTTATTTATTTTTCAAGTTCTCTGTTTGTTATGCGCCCGTAGGTGCAG
ACGAGGTTTGCACATGATGAACAGGGCTAAACGATGTTACAGCATTCTCGTTTATGACCTCCTGTTTTTTGTTCTGTAGCCGCTTGGCTTCATTATTTTCTACATATAACTTTGTGCAAACTTCTTCTGTACTCTTTTTAAAATTTTGATTATTACTGTGTCAAGAATCAAGTAACTATTATTATTATGATGATGATGGT
CTACCGTCG

Intron 2 GTGAGTGTGT ... ATGTGAACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	58.33	5	0	7
A2	-1	0	0	0
A3	-1	0	0	0
A4	0	1	0	0
A5	-1	0	0	0
A6	28.57	5	0	2
A7	40.63	19	2	13
A8	33.33	12	1	6
A9	11.11	16	0	2
A10	50	2	0	2

>TRINITY_DN32914_c0_g1_i1 from sample A7 / Example spliced isoform

TTGGCAAATCAATGAAGCTCTTACATTTCGGCTCGCAGGTTTTCGCACCGAGGAGAAATACTGACTGAACAGTGCTGTTCAAGCGTCTTTATTCACATCATAAGCACAGGGAAGGCAACAACAAACAACAACAGGAAGATGAATTTCTCTGGCTCTCGTTCTTGTTTTTCCGTATCTATATTTGGGTGTGCATTTCCTAT
TATGGCTAAGCAACTTGTTGGCACTTTGAGTCGATGGAATCATGCAGAGAGATGGCTGAGGCGAAAGAGGCCGGCGGCCCGAAGCGTGCTGGGAAGTAGCAATGTGAGATAAAGATTTGGAGGTGTGGGCATCAAGGCTCTCTATGTTTCTGTGTAGAGAGCAAAAGGGGAATGAAGCATTTGGTTTGCAATGCTGTAGA
TTTCGGTTCTGTAAATTCAGAGCTGCTTCTACAGCGACCTCTCCGATATATATTTATTTATTTTTCAAGTTCTCTGTTTGTTATGCGCCCGTAGGTGCAGACGAGGTTTGCACATGATGAACAGGGCTAAACGATGTTACAGCATTCTCGTTTATGACCTCCTGTTTTTTGTTCTGTAGCCGCTTGGCTTCATTATTTTC
TACATATAACTTTGTGCAAACTTCTTCTGTACTCTTTTTAAAATTTTGATTATTACTGTGTCAAGAATCAAGA

>TRINITY_DN32914_c0_g1_i2 from sample A7 / Example unspliced isoform:

TTGGCAAATCAATGAAGCTCTTACATTTCGGCTCGCAGGTTTTCGCACCGAGGAGAAATACTGACTGAACAGTGCTGTTCAAGCGTCTTTATTCACATCATAAGCACAGGgtgagtgtgtgttgtcttcctattttcatccttaaatctccactgggggttgggggttgcgtttttttgttgctcaacttaatgtcatgt
catgtgaacagGAAGGCAACAACAAACAACAACAGGAAGATGAATTTCTCTGGCTCTCGTTCTTGTTTTTCCGTATCTATATTTGGGTGTGCATTTCCTATTATGGCTAAGCAACTTGTTGGCACTTTGAGTCGATGGAATCATGCAGAGAGATGGCTGAGGCGAAAGAGGCCGGCGGCCCGAAGCGTGCTGGGAAGTAG
CAATGTGAGATAAAGATTTGGAGGTGTGGGCATCAAGGCTCTCTATGTTTCTGTGTAGAGAGCAAAAGGGGAATGAAGCATTTGGTTTGCAATGCTGTAGATTTCGGTTCTGTAAATTCAGAGCTGCTTCTACAGCGACCTCTCCGATATATATTTATTTATTTTTCAAGTTCTCTGTTTGTTATGCGCCCGTAGGTGCA
GACGAGGTTTGCACATGATGAACAGGGCTAAACGATGTTACAGCATTCTCGTTTATGACCTCCTGTTTTTTGTTCTGTAGCCGCTTGGCTTCATTATTTTCTACATATAACTTTGTGCAAACTTCTTCTGTACTCTTTTTAAAATTTTGATTATTACTGTGTCAAGAATCAAGA