sp|P53780|METC_ARATH ----> Cystathionine beta-lyase, chloroplastic

Intron 1 GTAGTGCATT ... CAAACTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	19.81	85	25	17
A2	25.63	74	24	27
A3	19.77	71	14	21
A4	17.2	65	18	9
A5	20.83	38	10	10
A6	19.88	135	40	27
A7	16.09	232	47	42
A8	16.78	119	23	25
A9	16.3	190	33	41
A10	12.02	183	27	23

>TRINITY_DN112554_c0_g1_i7 from sample A2 / Example spliced isoform

TACATGTTTAAAAGCTTTGTCTTGGAGGGGTCAATTTTGGTCACCGAGACTGGCTTCAAGAAAGAGAGAGGAGTTACGTATTCCTTGCAACTTGCAAGTACCTAGTTGATGTCACGGTGGCCGTGAACGTGACCGTGCTGAATGTCTCACCATTGATGATCACGGCGGTTCGACAATAATGTAAACATTTAAACCCTAAT
TTAAATTTAGCATCCTATATTTCAGTCGACTGCACTGCATGGGCTGGGTTCTCTGGATACCCAAGCTGGAGAGTTTCGTTTCAGGCTTTCAATTGCTTAGCCATCCATGGCCATGGCCGCAGCGCAGAACCCGGCAATGCATGGATTGAAACAACACAATTTACAAATCGGCCAGGGCTTTATCCACAACCCGCCTTCGA
GTGGATTCCGTTGGTCTCAGGAGTCGTGTAGAAATCGCAGCTTTACAACATTATCGGTAAGGTGCAGCAGTGACAAGGAGCTTCAGCTTACAATGGCTGCCATTCCTAACAATGACACAGATTGCGCTGCAGAGGATTCGTCCGCTTCTTTGTGTGAAAATGGTGTGCCAGAAAAAGAGTTGAGCATTTCTACAATTCTT
GTGAACTTCAGCAATTCTTTTGACCCATATGGCGCTCTAAGCACACCTCTCTACCAGACTGCAACTTTCAAGCAGCCATCGGCCACAGAGAATGGTCCATATGATTATACTAGAAGTGGAAATCCCACTCGGGATTCATTGGAAGATCTGTTAGCAAAGTTAGAGGGTGCCGATCGAGCATTTTGTTTCACAAGTGGGAT
GGCTGCTTTGTCTGCTGTTACACATCTTGTAGAAGCAGGACAGGAAATTGTTGCGGGAGATGACATATATGGAGGGTCTGATAGATTGCTTTCTAGAGTAGTCCCAAGAGATGGGATCATAGTAAAACGGGTTGACACAACTAATGTCGAAGAAGTGGCCGCGGCTGTTGGTCCATTGACAAAAGTTGTGTGGCTAGAGA
GTCCAACTAATCCACGTCAGAGAATATCTGATATACGGAAAATTTCGGAAATAGCACATGCACATGGTGCACTGGTACTTGTTGACAACAGTATCATGTCTCCTGTACTATCAAGACCGTTGGAGCTTGGAGCAGATATTGTGATGCATTCAGCAACGAAATTCATTGCTGGGCATAGTGATGTCATGGCAGGCGTTCTT
GCTGTAAAGGGGGAAAGTTTGGCCAAAGATATCTATTTCCTACAGAATGCAGAAGGTTCAGGTCTAGCTCCATTTGACTGTTGGCTATGTTTGCGAGGGATCAAGACAATGGCTTTGCGTATTGAGAAACAACAGGTGCCACACCTTTTAAAATTAGGCTTGAATATTCTTGTTTCTGAAAAGTCACATGGTCCTGATAT
CAATTTCTAGA

>TRINITY_DN112554_c0_g1_i1 from sample A2 / Example unspliced isoform:

TACATGTTTAAAAGCTTTGTCTTGGAGGGGTCAATTTTGGTCACCGAGACTGGCTTCAAGAAAGAGAGAGGAGTTACGTATTCCTTGCAACTTGCAAGTACCTAGTTGATGTCACGGTGGCCGTGAACGTGACCGTGCTGAATGTCTCACCATTGATGATCACGGCGGTTCGACAATAATGTAAACATTTAAACCCTAAT
TTAAATTTAGCATCCTATATTTCAGTCGACTGCACTGCATGGGCTGGGTTCTCTGGATACCCAAGCTGGAGAGTTTCGTTTCAGGCTTTCAATTGCTTAGCCATCCATGGCCATGGCCGCAGCGCAGAACCCGGCAATGCATGGATTGAAACAACACAATTTACAAATCGGCCAGGGCTTTATCCACAACCCGCCTTCGA
GTGGATTCCGTTGGTCTCAGGAGTCGTGTAGAAATCGCAGCTTTACAACATTATCGGTAAGGTGCAGCAGTGACAAGGAGCTTCAGCTTACAATGGCTGCCATTCCTAACAATGACACAGATTGCGCTGCAGAGGATTCGTCCGCTTCTTTGTGTGAAAATGGTGTGCCAGAAAAAGAGTTGAGCATTTCTACAATTCTT
GTGAACTTCAGCAATTCTTTTGACCCATATGGCGCTCTAAGCACACCTCTCTACCAGACTGCAACTTTCAAGCAGCCATCGGCCACAGAGAATGGTCCATATGATTATACTAGAAGTGGAAATCCCACTCGGGATTCATTGGAAGATCTGTTAGCAAAGTTAGAGGGTGCCGATCGAGCATTTTGTTTCACAAGTGGGAT
GGCTGCTTTGTCTGCTGTTACACATCTTGTAGAAGCAGgtagtgcattgctggctcatgtagattaaaaattgtccttttcttctgtagtttgaaaaatttgtatttacttttaaagtagtgttttgcaaactgcagGACAGGAAATTGTTGCGGGAGATGACATATATGGAGGGTCTGATAGATTGCTTTCTAGAGTAG
TCCCAAGAGATGGGATCATAGTAAAACGGGTTGACACAACTAATGTCGAAGAAGTGGCCGCGGCTGTTGGTCCATTGACAAAAGTTGTGTGGCTAGAGAGTCCAACTAATCCACGTCAGAGAATATCTGATATACGGAAAATTTCGGAAATAGCACATGCACATGGTGCACTGGTACTTGTTGACAACAGTATCATGTCT
CCTGTACTATCAAGACCGTTGGAGCTTGGAGCAGATATTGTGATGCATTCAGCAACGAAATTCATTGCTGGGCATAGTGATGTCATGGCAGGCGTTCTTGCTGTAAAGGGGGAAAGTTTGGCCAAAGATATCTATTTCCTACAGAATGCAGAAGGTTCAGGTCTAGCTCCATTTGACTGTTGGCTATGTTTGCGAGGGAT
CAAGACAATGGCTTTGCGTATTGAGAAACAACAGGAGAATGCGAGGAAAATTGCAGAATTTTTGTCAACTCATCCTCTAATTAAGGAAGTATATTATGCTGGCCTTCCTGGTCACCAAGGCCACAGTTTACATTTTTCACAGGCAAAGGGAGGAGGTTCAGTTCTTAGCTTTCAAACTGGTTCTTTGGCTCTTTCAAAGC
ACATCGTCGAAAACACAAGACTTTTCAGCATAACTGTTAGTTTTGGCAGTGTGAAGTCACTCATCAGTCTTCCATGTTTCATGTCACATGCAAGTATACCCCCTGAAGTGCGAAAGTCGCGGGGATTAACTGAAGACCTAATTCGCCTTTCTGTAGGGATTGAGGATGCAGTAGACCTGATTGCCGATATAGACCAAGCA
ATCAAATCTGCATCTGTCTAATACCAGCTAATTGATACCTTGGAAACAGGTTATGTAAGAATTTGGGCCATTTGCTTCCCTGTAGACCAAGTAAAATCTGAAATGGACATTTGTCGAGTTCATGAGGTTGTTTGTGCACAATATAAGCCATTGGCATCTAAGAAACTGGAGACCTTGCTTGTATGATCAACCTAGTTCCA
ACAATTTGCATATGGTGAATTATAGAGCTACTAGTCTTTTTGTGGATGATATATAATAGGTGGTGAAAAGTAGATCAGTTTTCTTGAAATAATTGACATATCCATAAATTAATCAGAAATTGATCATTAGGGCAGTTTCTTCTCCTGCGTAGAAAAAAATTCTGGTTTGGCAAATATAAGTCTGCCATCTCCAGAAGGAT
GAATGTGTAGATCCTTGTAGAGATGACGATATTTCCATTAGGCTGTAGCAATGCCCACATTTAATTTGACAGAAAAATTCAAACATGGTATTAAATTGTTTAGAAACTAGAATATCAGAAAGTCTAAATGCTAAAATTTAAAGAGAATTTGTTTTCGCCATATTTTAAGAACTTCTGTTCAGAGAGATTCCTTTATC

Intron 2 GTAAGTATCT ... TTTTATACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	27.29	56.3	6	21
A2	12.95	50.7	1	7
A3	25.28	60.3	3	20
A4	12.04	52.3	0	7
A5	23.71	29	1	8
A6	21.62	88.7	0	24
A7	21.71	156	0	42
A8	19.53	76.7	0	18
A9	22.34	119.3	0	33
A10	26.62	130	0	46

>TRINITY_DN115604_c4_g2_i6 from sample A1 / Example spliced isoform

GTCAAGACCAGTTACTAATTGATGGGCATGAGAGAGATTTTAATTTCCTATTAGTTCTCATCATTTCCCCGTACAATAAGGAGGAGATCTACAACTCACTTTTCAAAACATTTCAAACCAAAGGTTCTGTGCTAAAGTACATAATAGCTTTTGCTTTGATAACCTATATCAGAGATGTAGCATCTTTAGATGCCATAGAC
CTTGCAATTGTTTAATAGAAAATGTCACCTGCAAATCATAAGTGAAATGAGGAAATTTATATTATCTCCCATATGCAGCCATCTAATTTCTAATTGAATGTGGCATGTGATATTTGGAAACCAAGGCCTCTATTTCCTAGCTCATCATACAGTGGCACAATTTTGTTTGCATCTTTTCACAACTGTGGAATTCTTATTTG
CTTGCAGCCATCGGCCACAGAGAATGGTCCATATGATTATACTAGAAGTGGAAATCCCACTCGGGATTCATTGGAAGATCTGTTAGCAAAGTTAGAGGGTGCCGATCGAGCATTTTGTTTCACAAGTGGGATGGCTGCTTTGTCTGCTGTTACACATCTTGTAGAAGCAGGACAGGAAATTGTTGCGGGAGATGACATAT
ATGGAGGGTCTGATAGATTGCTTTCTAGAGTAGTCCCAAGAGATGGGATCATAGTAAAACGGGTTGACACAACTAATGTCGAAGAAGTGGCCGCGGCTGTTGGTCCATTGACAAAAGTTGTGTGGCTAGAGAGTCCAACTAATCCACGTCAGAGAATATCTGATATACGGAAAATTTCGGAAATAGCACATGCACATGGT
GCACTGGTACTTGTTGACAACAGTATCATGTCTCCTGTACTATCAAGACCGTTGGAGCTTGGAGCAGATATTGTGATGCATTCAGCAACGAAATTCATTGCTGGGCATAGTGATGTCATGGCAGGCGTTCTTGCTGTAAAGGGGGAAAGTTTGGCCAAAGATATCTATTTCCTACAGAATGCAGAAGGTTCAGGTCTAGC
TCCATTTGACTGTTGGCTATGTTTGCGAGGGATCAAGACAATGGCTTTGCGTATTGAGAAACAACAGGAGAATGCGAGGAAAATTGCAGAATTTTTGTCAACTCATCCTCTAATTAAGGAAGTATATTATGCTGGCCTTCCTGGTCACCAAGGCCACAGTTTACATTTTTCACAGGCAAAGGGAGGAGGTTCAGTTCTTA
GCTTTCAAACTGGTTCTTTGGCTCTTTCAAAGCACATCGTCGAAAACACAAGACTTTTCAGCATAACTGTTAGTTTTGGCAGTGTGAAGTCACTCATCAGTCTTCCATGTTTCATGTCACATGCAAGTATACCCCCTGAAGTGCGAAAGTCGCGGGGATTAACTGAAGACCTAATTCGCCTTTCTGTAGGGATTGAGGAT
GCAGTAGACCTGATTGCCGATATAGACCAAGCAATCAAATCTGCATCTGTCTAATACCAGCTAATTGATACCTTGGAAACAGGTTATGTAAGAATTTGGGCCATTTGCTTCCCTGTAGACCAAGTAAAATCTGAAATGGACATTTGTCGAGTTCATGAGGTTGTTTGTGCACAATATAAGCCATTGGCATCTAAGAAACT
GGAGACCTTGCTTGTATGATCAACCTAGTTCCAACAATTTGCATATGGTGAATTATAGAGCTACTAGTCTTTTTGTGGATGATATATAATAGGTGGTGAAAAGTAGATCAGTTTTCTTGAAATAATTGACATATCCATAAATTAATCAGAAATTGATCATTAGGGCAGTTTCTTCTCCTGCGTAGAAAAAAATTCTGGTT
TGGCAAATATAAGTCTGCCATCTCCAGAAGGATGAATGTGTAGATCCTTGTAGAGATGACGATATTTCCATTAGGCTGTAGCAATGCCCACATTTAATTTGACAGAAAAATTCAAACATGGTATTAAATTGTTTAGAAACTAGAATATCAGAAAGTCTAAATGCTAAAATTTAAAGAGAATTTGTTTTCGC

>TRINITY_DN115604_c4_g2_i2 from sample A1 / Example unspliced isoform:

GTCAAGACCAGTTACTAATTGATGGGCATGAGAGAGATTTTAATTTCCTATTAGTTCTCATCATTTCCCCGTACAATAAGGAGGAGATCTACAACTCACTTTTCAAAACATTTCAAACCAAAGGTTCTGTGCTAAAGTACATAATAGCTTTTGCTTTGATAACCTATATCAGAGATGTAGCATCTTTAGATGCCATAGAC
CTTGCAATTGTTTAATAGAAAATGTCACCTGCAAATCATAAGTGAAATGAGGAAATTTATATTATCTCCCATATGCAGCCATCTAATTTCTAATTGAATGTGGCATGTGATATTTGGAAACCAAGGCCTCTATTTCCTAGCTCATCATACAGTGGCACAATTTTGTTTGCATCTTTTCACAACTGTGGAATTCTTATTTG
CTTGCAGCCATCGGCCACAGAGAATGGTCCATATGATTATACTAGAAGTGGAAATCCCACTCGGGATTCATTGGAAGAgtaagtatctttggattattatgactcatatttgttagctccgagaaagcgcatgtatacattatatgttagttgtctggttgggttctttagcttaagaaggtaacatttggctttataat
attctgtgcaaatttgtgttccataagcaattgagaatattttttatacagTCTGTTAGCAAAGTTAGAGGGTGCCGATCGAGCATTTTGTTTCACAAGTGGGATGGCTGCTTTGTCTGCTGTTACACATCTTGTAGAAGCAGGACAGGAAATTGTTGCGGGAGATGACATATATGGAGGGTCTGATAGATTGCTTTCTA
GAGTAGTCCCAAGAGATGGGATCATAGTAAAACGGGTTGACACAACTAATGTCGAAGAAGTGGCCGCGGCTGTTGGTCCATTGACAAAAGTTGTGTGGCTAGAGAGTCCAACTAATCCACGTCAGAGAATATCTGATATACGGAAAATTTCGGAAATAGCACATGCACATGGTGCACTGGTACTTGTTGACAACAGTATC
ATGTCTCCTGTACTATCAAGACCGTTGGAGCTTGGAGCAGATATTGTGATGCATTCAGCAACGAAATTCATTGCTGGGCATAGTGATGTCATGGCAGGCGTTCTTGCTGTAAAGGGGGAAAGTTTGGCCAAAGATATCTATTTCCTACAGAATGCAGAAGGTTCAGGTCTAGCTCCATTTGACTGTTGGCTATGTTTGCG
AGGGATCAAGACAATGGCTTTGCGTATTGAGAAACAACAGGAGAATGCGAGGAAAATTGCAGAATTTTTGTCAACTCATCCTCTAATTAAGGAAGTATATTATGCTGGCCTTCCTGGTCACCAAGGCCACAGTTTACATTTTTCACAGGCAAAGGGAGGAGGTTCAGTTCTTAGCTTTCAAACTGGTTCTTTGGCTCTTT
CAAAGCACATCGTCGAAAACACAAGACTTTTCAGCATAACTGTTAGTTTTGGCAGTGTGAAGTCACTCATCAGTCTTCCATGTTTCATGTCACATGCAAGTATACCCCCTGAAGTGCGAAAGTCGCGGGGATTAACTGAAGACCTAATTCGCCTTTCTGTAGGGATTGAGGATGCAGTAGACCTGATTGCCGATATAGAC
CAAGCAATCAAATCTGCATCTGTCTAATACCAGCTAATTGATACCTTGGAAACAGGTTATGTAAGAATTTGGGCCATTTGCTTCCCTGTAGACCAAGTAAAATCTGAAATGGACATTTGTCGAGTTCATGAGGTTGTTTGTGCACAATATAAGCCATTGGCATCTAAGAAACTGGAGACCTTGCTTGTATGATCAACCTA
GTTCCAACAATTTGCATATGGTGAATTATAGAGCTACTAGTCTTTTTGTGGATGATATATAATAGGTGGTGAAAAGTAGATCAGTTTTCTTGAAATAATTGACATATCCATAAATTAATCAGAAATTGATCATTAGGGCAGTTTCTTCTCCTGCGTAGAAAAAAATTCTGGTTTGGCAAATATAAGTCTGCCATCTCCAG
AAGGATGAATGTGTAGATCCTTGTAGAGATGACGATATTTCCATTAGGCTGTAGCAATGCCCACATTTAATTTGACAGAAAAATTCAAACATGGTATTAAATTGTTTAGAAACTAGAATATCAGAAAGTCTAAATGCTAAAATTTAAAGAGAATTTGTTTTCGC

Intron 3 GTAAACATTT ... TTCGTTTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	53.27	93	83	129
A2	63.78	46	62	100
A3	58.84	64	79	104
A4	64.52	44	63	97
A5	72.8	17	28	63
A6	62.6	46	59	95
A7	57.72	141	147	238
A8	61.03	38	42	77
A9	55.51	101	91	161
A10	58.19	97	102	168

>TRINITY_DN112554_c0_g1_i6 from sample A2 / Example spliced isoform

TACATGTTTAAAAGCTTTGTCTTGGAGGGGTCAATTTTGGTCACCGAGACTGGCTTCAAGAAAGAGAGAGGAGTTACGTATTCCTTGCAACTTGCAAGTACCTAGTTGATGTCACGGTGGCCGTGAACGTGACCGTGCTGAATGTCTCACCATTGATGATCACGGCGGTTCGACAATAATGCTTTCAATTGCTTAGCCAT
CCATGGCCATGGCCGCAGCGCAGAACCCGGCAATGCATGGATTGAAACAACACAATTTACAAATCGGCCAGGGCTTTATCCACAACCCGCCTTCGAGTGGATTCCGTTGGTCTCAGGAGTCGTGTAGAAATCGCAGCTTTACAACATTATCGGTAAGGTGCAGCAGTGACAAGGAGCTTCAGCTTACAATGGCTGCCATT
CCTAACAATGACACAGATTGCGCTGCAGAGGATTCGTCCGCTTCTTTGTGTGAAAATG

>TRINITY_DN112554_c0_g1_i2 from sample A2 / Example unspliced isoform:

TACATGTTTAAAAGCTTTGTCTTGGAGGGGTCAATTTTGGTCACCGAGACTGGCTTCAAGAAAGAGAGAGGAGTTACGTATTCCTTGCAACTTGCAAGTACCTAGTTGATGTCACGGTGGCCGTGAACGTGACCGTGCTGAATGTCTCACCATTGATGATCACGGCGGTTCGACAATAATgtaaacatttaaaccctaat
ttaaatttagcatcctatatttcagtcgactgcactgcatgggctgggttctctggatacccaagctggagagtttcgtttcagGCTTTCAATTGCTTAGCCATCCATGGCCATGGCCGCAGCGCAGAACCCGGCAATGCATGGATTGAAACAACACAATTTACAAATCGGCCAGGGCTTTATCCACAACCCGCCTTCGA
GTGGATTCCGTTGGTCTCAGGAGTCGTGTAGAAATCGCAGCTTTACAACATTATCGGTAAGGTGCAGCAGTGACAAGGAGCTTCAGCTTACAATGGCTGCCATTCCTAACAATGACACAGATTGCGCTGCAGAGGATTCGTCCGCTTCTTTGTGTGAAAATGGTGTGCCAGAAAAAGAGTTGAGCATTTCTACAATTCTT
GTGAACTTCAGCAATTCTTTTGACCCATATGGCGCTCTAAGCACACCTCTCTACCAGACTGCAACTTTCAAGCAGCCATCGGCCACAGAGAATGGTCCATATGATTATACTAGAAGTGGAAATCCCACTCGGGATTCATTGGAAGAGACAGGAAATTGTTGCGGGAGATGACATATATGGAGGGTCTGATAGATTGCTTT
CTAGAGTAGTCCCAAGAGATGGGATCATAGTAAAACGGGTTGACACAACTAATGTCGAAGAAGTGGCCGCGGCTGTTGGTCCATTGACAAAAGTTGTGTGGCTAGAGAGTCCAACTAATCCACGTCAGAGAATATCTGATATACGGAAAATTTCGGAAATAGCACATGCACATGGTGCACTGGTACTTGTTGACAACAGT
ATCATGTCTCCTGTACTATCAAGACCGTTGGAGCTTGGAGCAGATATTGTGATGCATTCAGCAACGAAATTCATTGCTGGGCATAGTGATGTCATGGCAGGCGTTCTTGCTGTAAAGGGGGAAAGTTTGGCCAAAGATATCTATTTCCTACAGAATGCAGAAGGTTCAGGTCTAGCTCCATTTGACTGTTGGCTATGTTT
GCGAGGGATCAAGACAATGGCTTTGCGTATTGAGAAACAACAGGAGAATGCGAGGAAAATTGCAGAATTTTTGTCAACTCATCCTCTAATTAAGGAAGTATATTATGCTGGCCTTCCTGGTCACCAAGGCCACAGTTTACATTTTTCACAGGCAAAGGGAGGAGGTTCAGTTCTTAGCTTTCAAACTGGTTCTTTGGCTC
TTTCAAAGCACATCGTCGAAAACACAAGACTTTTCAGCATAACTGTTAGTTTTGGCAGTGTGAAGTCACTCATCAGTCTTCCATGTTTCATGTCACATGCAAGTATACCCCCTGAAGTGCGAAAGTCGCGGGGATTAACTGAAGACCTAATTCGCCTTTCTGTAGGGATTGAGGATGCAGTAGACCTGATTGCCGATATA
GACCAAGCAATCAAATCTGCATCTGTCTAATACCAGCTAATTGATACCTTGGAAACAGGTTATGTAAGAATTTGGGCCATTTGCTTCCCTGTAGACCAAGTAAAATCTGAAATGGACATTTGTCGAGTTCATGAGGTTGTTTGTGCACAATATAAGCCATTGGCATCTAAGAAACTGGAGACCTTGCTTGTATGATCAAC
CTAGTTCCAACAATTTGCATATGGTGAATTATAGAGCTACTAGTCTTTTTGTGGATGATATATAATAGGTGGTGAAAAGTAGATCAGTTTTCTTGAAATAATTGACATATCCATAAATTAATCAGAAATTGATCATTAGGGCAGTTTCTTCTCCTGCGTAGAAAAAAATTCTGGTTTGGCAAATATAAGTCTGCCATCTC
CAGAAGGATGAATGTGTAGATCCTTGTAGAGATGACGATATTTCCATTAGGCTGTAGCAATGCCCACATTTAATTTGACAGAAAAATTCAAACATGGTATTAAATTGTTTAGAAACTAGAATATCAGAAAGTCTAAATGCTAAAATTTAAAGAGAATTTGTTTTCGCCATATTTTAAGAACTTCTGTTCAGAGAGATTCC
TTTATC