FastQCFastQC Report
Mon 21 Jul 2025
SCP03_MKRN250024229-1A_22VTJNLT4_L5_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSCP03_MKRN250024229-1A_22VTJNLT4_L5_2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6249680
Sequences flagged as poor quality0
Sequence length150
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGACT12650.019.3540211
CCTAGCT7150.019.1350841
GTTCGGG6251.8189894E-1218.4341351
GCCGGCT4304.0543455E-818.420741
GTCCGTA29650.017.4860061
TTCCCCT36600.016.7205737
GCGACTT12500.016.7037982
CCGTACC31150.016.6414553
TCCGTAC30750.016.624192
CTATGCG8300.016.48128
GTCGGAT16300.016.3454511
GGCGGCT4752.3092816E-615.1596531
GCGGTAT9050.015.1177751
TATGCGA9600.014.9993389
GTGCAAT31950.014.1988281
GGCGGCG3050.005493776414.1655761
TTGTCCG32750.014.0700592
GTCCGAT5709.980831E-713.8963471
CCAACGC38800.013.7313548
ATTCCCC45200.013.6985116
Produced by FastQC (version 0.11.5)