FastQCFastQC Report
Mon 21 Jul 2025
SCP03_MKRN250024229-1A_22VTJNLT4_L5_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSCP03_MKRN250024229-1A_22VTJNLT4_L5_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6249680
Sequences flagged as poor quality0
Sequence length150
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCACT1352.2417635E-532.0127371
GGCGACT12450.025.4559141
GCGACTT12950.023.3604582
TTCTCGC8600.017.5798116
GTCCGTA24300.017.4884411
TCCGTAC24350.017.1565882
CCGTACC24100.017.0281933
TCTCGCA8050.016.9922587
GCCGGAT7753.637979E-1215.7998361
CGCACTA3656.310467E-515.7869782
TCGCATC8350.015.5195599
CGACTTA20550.015.0649383
GTCGGAA33550.015.0283361
TTGTCCG26950.014.9668752
TCCTCGC3508.23863E-414.3987027
TTCCCCT27800.014.243327
GTGCAAT29300.013.7665691
TCCGATC7951.3551471E-913.5842243
GCGAATC9054.7293724E-1113.5301372
GACTTAA21850.013.509624
Produced by FastQC (version 0.11.5)