FastQCFastQC Report
Mon 21 Jul 2025
SCP03_MKRN250024229-1A_22VNWKLT4_L6_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSCP03_MKRN250024229-1A_22VNWKLT4_L6_2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17987560
Sequences flagged as poor quality0
Sequence length150
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCGGA4350.024.8276651
TCCGCGC1751.3183108E-424.683052
CCTAGCT20750.023.5952571
GGCGACT35350.021.5898861
GCGGACT2701.1393315E-418.6667271
CTCCGCG1950.00712368318.4615971
GTCGGAT48950.018.3861681
GCGACTT35550.018.023372
GTCCGTA88500.017.5729391
CGCGCTG2901.9718603E-417.3760838
TCCGTAC91250.017.1203422
CGCGTAG3403.4494748E-516.9412331
CCGTACC92650.016.938033
CTATGCG22100.016.9380288
GCCGGCT13250.016.8453371
TTCCCCT103300.015.6098797
TCTCGCA20350.015.2109337
TTCTCGC21700.013.6011596
CCCGAGT44650.013.5453981
GCGCACT4804.2771913E-513.5000441
Produced by FastQC (version 0.11.5)