FastQCFastQC Report
Mon 21 Jul 2025
SCP03_MKRN250024229-1A_22VNWKLT4_L6_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSCP03_MKRN250024229-1A_22VNWKLT4_L6_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17987560
Sequences flagged as poor quality0
Sequence length150
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGACT32000.025.6619261
CGCGCTG3052.1957021E-823.605788
GCGACTT31900.022.1293432
TTCTCGC24850.018.542656
TCTCGCA24300.017.4809077
GTCCGTA70250.017.329051
TCGCATC23700.017.012059
CCGTACC73250.016.3208143
TCCGTAC72250.016.1514052
TTCCCCT85350.015.5214597
TCCGATC22900.015.4099763
GTCGGAA104150.015.354231
CCTAGCT20300.014.9034771
GCCACAC121400.014.8932481
GCGGGGT4953.5321227E-614.5522171
CTCGCAT28850.014.4743948
GTCGGAT45050.014.3907051
GTGCAAT87750.013.8731141
CCCGAGT37500.013.8304271
GTTCTCG33400.013.5803175
Produced by FastQC (version 0.11.5)