FastQCFastQC Report
Mon 21 Jul 2025
SCP02_MKRN250024228-1A_22VTJNLT4_L5_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSCP02_MKRN250024228-1A_22VTJNLT4_L5_2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19190562
Sequences flagged as poor quality0
Sequence length150
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCGGA6400.032.6294981
GTCGCGC2353.9131333E-521.4497661
GCCGGCT12850.020.7343751
TCGCGCA2506.309776E-520.1596262
CGCGCTG1800.004487226319.9990568
GGCGACT34450.019.6485711
CGCGTGA3302.6666978E-517.4569511
GTCGGAT63450.016.9101311
TCCGTAC89400.016.6708322
GCGACTT36450.016.5922852
CGCGTGT3504.4172597E-516.4594121
GTCCGTA90850.016.4073241
CCGTACC92500.016.0338783
CCCGGCC4651.8542723E-615.483183
TTCCCCT107500.015.2699767
GGCGCAC5706.049777E-815.1599851
TTCTCGC22500.015.039296
TCTCGCA22300.014.8513167
GTGCAAT97300.014.8016281
TCGCATC23600.013.1180249
Produced by FastQC (version 0.11.5)