FastQCFastQC Report
Mon 21 Jul 2025
SCP02_MKRN250024228-1A_22VTJNLT4_L5_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSCP02_MKRN250024228-1A_22VTJNLT4_L5_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19190562
Sequences flagged as poor quality0
Sequence length150
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGACT35000.026.1373771
GCGACTT35150.024.1814252
TTCGCGC1500.001557613123.9977027
GTGCAAT96950.016.6428071
GTCGGAT54200.016.3467771
GTCGGAA107650.016.0591871
GTCCGTA68900.015.4727971
CCGTACC67100.015.3435933
TCCGTAC68800.015.0764562
CGACTTA57300.014.7009293
TTCCCCT86700.014.5314827
GCCACAC121650.012.9083491
CGTATGC77900.012.846683
TCTCGCA24950.012.6961797
GACTTAA70250.012.1959094
CCCGCTT9059.273208E-911.939031
TTCTCGC24750.011.9261316
GTCCTAG31850.011.5341891
GGCGCAC5009.061198E-411.5251431
ACTTAAG84150.011.2930365
Produced by FastQC (version 0.11.5)