FastQCFastQC Report
Mon 21 Jul 2025
SCP02_MKRN250024228-1A_22VNWKLT4_L6_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSCP02_MKRN250024228-1A_22VNWKLT4_L6_2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19087678
Sequences flagged as poor quality0
Sequence length150
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCGGA4700.030.638931
CTCGCGC1400.001041891325.7119182
CCTCGCG1750.003810429520.5718541
GGCGACT34450.019.8552741
GCGACTT37750.018.8803832
GCCGGCT11500.018.7829971
CCCGGCC4654.9276423E-918.5770853
GTCCGTA90900.018.297411
TCCGTAC92300.017.8618452
CCGTACC93800.017.574463
GCCTAGC15700.016.5098951
TCGCGCA2750.00276550715.7060823
TTCCCCT103750.015.333937
CGCGTGT3355.9069146E-415.0450871
CTATGCG21050.014.7050238
GTGCAAT95850.014.4979521
GCGGGCC3650.001130708813.8085041
GTCGGAT61250.013.7537551
CCCCGGC7801.5123078E-812.9218872
TTCTCGC23650.012.7840216
Produced by FastQC (version 0.11.5)