FastQCFastQC Report
Mon 21 Jul 2025
SCP02_MKRN250024228-1A_22VNWKLT4_L6_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSCP02_MKRN250024228-1A_22VNWKLT4_L6_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19087678
Sequences flagged as poor quality0
Sequence length150
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGACT33350.024.6226581
GCGACTT36600.020.468132
GTCGGAA108800.017.9418131
GTGCAAT96450.015.907551
GTCCGTA67800.015.8300571
CCGTACC64600.015.7187113
TCCGTAC67150.015.6614792
GTCGGAT54250.015.1366941
GCGCACT3859.945872E-514.9677031
TTCCCCT84050.014.9047497
TCTCGCA24050.014.6687857
TTCTCGC23950.014.42941956
GCCACAC121150.014.1507491
TCGCATC23650.014.0035669
CGACTTA54900.013.2488853
AATCCGC140100.012.7446146
TAGCGTG45950.012.691498
CCTAGCT21250.012.5420551
GTCCTAG30550.012.4965631
ATCCGCT144900.012.0739977
Produced by FastQC (version 0.11.5)