FastQCFastQC Report
Mon 21 Jul 2025
SCP01_MKRN250024227-1A_22VTJNLT4_L5_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSCP01_MKRN250024227-1A_22VTJNLT4_L5_2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25638563
Sequences flagged as poor quality0
Sequence length150
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGA4450.027.508441
TCCGTAC113750.018.9249972
GCCGGCT18300.018.8871821
GTCCGTA117900.018.3835641
GGCGACT45100.018.041761
CCGTACC119350.018.0369153
GCGGGGC5552.391971E-916.8665941
GCGACTT47450.015.1732912
GTCGGAT83950.015.0104731
GCCCGGA6251.1530574E-814.9775361
TTCCCCT135150.014.8628357
GTGCAAT132000.014.8378851
CGCGGAT3406.605489E-414.8250481
CTATGCG33950.014.6326448
GCGGGGG9355.456968E-1213.8623841
GCCACAC156000.013.8475731
GTCGGAA128700.013.8195971
AGCGCTC4753.8778584E-513.6415882
GCCCGAT12750.012.9895671
CGGCCTA4503.750705E-412.7995142
Produced by FastQC (version 0.11.5)