FastQCFastQC Report
Mon 21 Jul 2025
SCP01_MKRN250024227-1A_22VTJNLT4_L5_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSCP01_MKRN250024227-1A_22VTJNLT4_L5_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25638563
Sequences flagged as poor quality0
Sequence length150
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGACT45750.021.0978261
TCCGATC37850.019.4020393
GCGACTT49200.019.1791112
TTCTCGC33100.017.6177226
TCCGTAC85700.017.4825522
TCTCGCA34200.017.0510717
CCGTACC88100.016.6712173
GTCCGTA92000.016.5203271
GTCGGAT76900.016.2984731
CTATGCG39250.015.2240848
TCGCATC35750.015.1035129
GTGCAAT127250.014.88750651
ACCGCGG12750.014.6810099
TTCCCCT110850.014.0284867
CCTAGCT28300.013.99910451
CGATCTG21000.013.3702045
TATGCGA47150.012.9786639
TTGTCCG104950.012.7659412
GCCACAC148950.012.428441
CTCGCAT44800.012.3738678
Produced by FastQC (version 0.11.5)